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Q9D9J7

- IZUM1_MOUSE

UniProt

Q9D9J7 - IZUM1_MOUSE

Protein

Izumo sperm-egg fusion protein 1

Gene

Izumo1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Essential sperm cell-surface protein required for fertilization by acting as a ligand for FOLR4/JUNO receptor on egg. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.2 Publications

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. protein homodimerization activity Source: UniProtKB
    3. receptor binding Source: UniProtKB

    GO - Biological processi

    1. cell adhesion Source: HGNC
    2. fusion of sperm to egg plasma membrane Source: UniProtKB
    3. single fertilization Source: UniProtKB
    4. sperm-egg recognition Source: UniProtKB

    Keywords - Biological processi

    Fertilization

    Enzyme and pathway databases

    ReactomeiREACT_199081. Sperm:Oocyte Membrane Binding.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Izumo sperm-egg fusion protein 1
    Alternative name(s):
    Oocyte binding/fusion factor
    Short name:
    OBF
    Sperm-specific protein izumo
    Gene namesi
    Name:Izumo1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1920706. Izumo1.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal membrane Source: MGI
    2. acrosomal vesicle Source: MGI
    3. integral component of membrane Source: HGNC

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are healthy but the males are sterile. They produce morphologically normal sperm that can bind to and penetrate the zona pellucida but that are incapable of fusing with eggs.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi204 – 2041N → Q: Almost no change in fusion-facilitating activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 397376Izumo sperm-egg fusion protein 1PRO_0000045483Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi182 ↔ 233By similarity
    Glycosylationi204 – 2041N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.1 Publication
    Phosphorylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9D9J7.
    PRIDEiQ9D9J7.

    PTM databases

    PhosphoSiteiQ9D9J7.

    Expressioni

    Tissue specificityi

    Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.2 Publications

    Gene expression databases

    BgeeiQ9D9J7.
    CleanExiMM_IZUMO1.
    GenevestigatoriQ9D9J7.

    Interactioni

    Subunit structurei

    Monomer, homodimer and homooligomer. Interacts with FOLR4/JUNO.2 Publications

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000033100.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D9J7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 319298ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini341 – 39757CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei320 – 34021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini167 – 25185Ig-like C2-typeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Izumo family.Curated

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG41344.
    GeneTreeiENSGT00390000015014.
    HOGENOMiHOG000113123.
    HOVERGENiHBG081813.
    InParanoidiQ9D9J7.
    OMAiYRVWGNN.
    OrthoDBiEOG72ZCG1.
    PhylomeDBiQ9D9J7.
    TreeFamiTF338356.

    Family and domain databases

    InterProiIPR003599. Ig_sub.
    IPR029389. IZUMO.
    [Graphical view]
    PfamiPF15005. IZUMO. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D9J7-1 [UniParc]FASTAAdd to Basket

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    MGPHFTLLLA ALANCLCPGR PCIKCDQFVT DALKTFENTY LNDHLPHDIH    50
    KNVMRMVNHE VSSFGVVTSA EDSYLGAVDE NTLEQATWSF LKDLKRITDS 100
    DLKGELFIKE LLWMLRHQKD IFNNLARQFQ KEVLCPNKCG VMSQTLIWCL 150
    KCEKQLHICR KSLDCGERHI EVHRSEDLVL DCLLSWHRAS KGLTDYSFYR 200
    VWENSSETLI AKGKEPYLTK SMVGPEDAGN YRCVLDTINQ GHATVIRYDV 250
    TVLPPKHSEE NQPPNIITQE EHETPVHVTP QTPPGQEPES ELYPELHPEL 300
    YPELIPTVAQ NPEKKMKTRL LILLTLGFVV LVASIIISVL HFRKVSAKLK 350
    NASDEVKPTA SGSKSDQSLS QQMGLKKASQ ADFNSDYSGD KSEATEN 397
    Length:397
    Mass (Da):44,885
    Last modified:June 1, 2001 - v1
    Checksum:i70D10D480B78F5C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB195681 mRNA. Translation: BAD91011.1.
    AK006830 mRNA. Translation: BAB24761.1.
    CCDSiCCDS21255.1.
    RefSeqiNP_001018013.1. NM_001018013.1.
    UniGeneiMm.380445.

    Genome annotation databases

    EnsembliENSMUST00000033100; ENSMUSP00000033100; ENSMUSG00000064158.
    GeneIDi73456.
    KEGGimmu:73456.
    UCSCiuc009gwh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB195681 mRNA. Translation: BAD91011.1 .
    AK006830 mRNA. Translation: BAB24761.1 .
    CCDSi CCDS21255.1.
    RefSeqi NP_001018013.1. NM_001018013.1.
    UniGenei Mm.380445.

    3D structure databases

    ProteinModelPortali Q9D9J7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000033100.

    PTM databases

    PhosphoSitei Q9D9J7.

    Proteomic databases

    PaxDbi Q9D9J7.
    PRIDEi Q9D9J7.

    Protocols and materials databases

    DNASUi 73456.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033100 ; ENSMUSP00000033100 ; ENSMUSG00000064158 .
    GeneIDi 73456.
    KEGGi mmu:73456.
    UCSCi uc009gwh.1. mouse.

    Organism-specific databases

    CTDi 284359.
    MGIi MGI:1920706. Izumo1.

    Phylogenomic databases

    eggNOGi NOG41344.
    GeneTreei ENSGT00390000015014.
    HOGENOMi HOG000113123.
    HOVERGENi HBG081813.
    InParanoidi Q9D9J7.
    OMAi YRVWGNN.
    OrthoDBi EOG72ZCG1.
    PhylomeDBi Q9D9J7.
    TreeFami TF338356.

    Enzyme and pathway databases

    Reactomei REACT_199081. Sperm:Oocyte Membrane Binding.

    Miscellaneous databases

    NextBioi 338309.
    PROi Q9D9J7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D9J7.
    CleanExi MM_IZUMO1.
    Genevestigatori Q9D9J7.

    Family and domain databases

    InterProi IPR003599. Ig_sub.
    IPR029389. IZUMO.
    [Graphical view ]
    Pfami PF15005. IZUMO. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs."
      Inoue N., Ikawa M., Isotani A., Okabe M.
      Nature 434:234-238(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "Putative sperm fusion protein IZUMO and the role of N-glycosylation."
      Inoue N., Ikawa M., Okabe M.
      Biochem. Biophys. Res. Commun. 377:910-914(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-204, MUTAGENESIS OF ASN-204.
    4. "Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm."
      Ellerman D.A., Pei J., Gupta S., Snell W.J., Myles D., Primakoff P.
      Mol. Reprod. Dev. 76:1188-1199(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, TISSUE SPECIFICITY, PHOSPHORYLATION, GENE FAMILY, NOMENCLATURE.
    5. "Juno is the egg Izumo receptor and is essential for mammalian fertilization."
      Bianchi E., Doe B., Goulding D., Wright G.J.
      Nature 508:483-487(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH FOLR4.

    Entry informationi

    Entry nameiIZUM1_MOUSE
    AccessioniPrimary (citable) accession number: Q9D9J7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Izumo is the name of a Japanese shrine to marriage.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3