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Protein

Izumo sperm-egg fusion protein 1

Gene

Izumo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.3 Publications

GO - Molecular functioni

  • protein binding involved in heterotypic cell-cell adhesion Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: HGNC
  • fusion of sperm to egg plasma membrane Source: UniProtKB
  • heterotypic cell-cell adhesion Source: UniProtKB
  • single fertilization Source: UniProtKB
  • sperm-egg recognition Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Fertilization

Enzyme and pathway databases

ReactomeiR-MMU-1300652. Sperm:Oocyte Membrane Binding.

Names & Taxonomyi

Protein namesi
Recommended name:
Izumo sperm-egg fusion protein 1
Alternative name(s):
Oocyte binding/fusion factor
Short name:
OBF
Sperm-specific protein izumo
Gene namesi
Name:Izumo1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1920706. Izumo1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 319ExtracellularSequence analysisAdd BLAST298
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 397CytoplasmicSequence analysisAdd BLAST57

GO - Cellular componenti

  • acrosomal membrane Source: MGI
  • acrosomal vesicle Source: MGI
  • integral component of membrane Source: HGNC
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are healthy but the males are sterile. They produce morphologically normal sperm that can bind to and penetrate the zona pellucida but that are incapable of fusing with eggs.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148W → A: Abolishes adhesion to oocytes. 1 Publication1
Mutagenesisi154K → A: Decreases adhesion to oocytes. 1 Publication1
Mutagenesisi157H → A: Abolishes adhesion to oocytes. 1 Publication1
Mutagenesisi158I → R: Strongly decreases adhesion to oocytes. 1 Publication1
Mutagenesisi160R → A: Abolishes adhesion to oocytes. 1 Publication1
Mutagenesisi163L → A: Decreases adhesion to oocytes. 1 Publication1
Mutagenesisi204N → Q: Almost no change in fusion-facilitating activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004548322 – 397Izumo sperm-egg fusion protein 1Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 149By similarity
Disulfide bondi25 ↔ 152By similarity
Disulfide bondi135 ↔ 159By similarity
Disulfide bondi139 ↔ 165By similarity
Disulfide bondi182 ↔ 233By similarity
Glycosylationi204N-linked (GlcNAc...)1 Publication1
Modified residuei379PhosphoserineBy similarity1

Post-translational modificationi

N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.1 Publication
Phosphorylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9D9J7.
PaxDbiQ9D9J7.
PRIDEiQ9D9J7.

PTM databases

iPTMnetiQ9D9J7.
PhosphoSitePlusiQ9D9J7.

Expressioni

Tissue specificityi

Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.2 Publications

Gene expression databases

BgeeiENSMUSG00000064158.
CleanExiMM_IZUMO1.
GenevisibleiQ9D9J7. MM.

Interactioni

Subunit structurei

Monomer, homodimer and homooligomer (PubMed:19658160). Interacts with IZUMO1R/JUNO (PubMed:24739963, PubMed:27309808). IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (By similarity).By similarity3 Publications

GO - Molecular functioni

  • protein binding involved in heterotypic cell-cell adhesion Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033100.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 25Combined sources3
Helixi27 – 38Combined sources12
Helixi40 – 44Combined sources5
Helixi47 – 49Combined sources3
Helixi50 – 62Combined sources13
Turni63 – 65Combined sources3
Helixi70 – 75Combined sources6
Beta strandi76 – 79Combined sources4
Helixi80 – 99Combined sources20
Helixi104 – 131Combined sources28
Beta strandi138 – 148Combined sources11
Turni149 – 152Combined sources4
Beta strandi153 – 160Combined sources8
Beta strandi163 – 173Combined sources11
Beta strandi178 – 181Combined sources4
Helixi185 – 188Combined sources4
Beta strandi191 – 201Combined sources11
Beta strandi207 – 214Combined sources8
Beta strandi216 – 220Combined sources5
Helixi225 – 227Combined sources3
Beta strandi229 – 240Combined sources12
Beta strandi242 – 253Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B5KX-ray2.50A22-257[»]
ProteinModelPortaliQ9D9J7.
SMRiQ9D9J7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 251Ig-like C2-typeAdd BLAST85

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 160Important for interaction with IZUMO1R1 PublicationAdd BLAST13

Domaini

The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO. Interaction with IZUMO1R/JUNO triggers a conformation change, so that the IZUMO1 extracellular domain assumes an upright conformation.By similarity

Sequence similaritiesi

Belongs to the Izumo family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHEV. Eukaryota.
ENOG410Z8SJ. LUCA.
GeneTreeiENSGT00390000015014.
HOGENOMiHOG000113123.
HOVERGENiHBG081813.
InParanoidiQ9D9J7.
OMAiKEVHACR.
OrthoDBiEOG091G0AHL.
PhylomeDBiQ9D9J7.
TreeFamiTF338356.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR029389. IZUMO.
IPR032699. Izumo-Ig.
IPR032700. IZUMO1.
[Graphical view]
PANTHERiPTHR35540:SF1. PTHR35540:SF1. 1 hit.
PfamiPF15005. IZUMO. 1 hit.
PF16706. Izumo-Ig. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D9J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPHFTLLLA ALANCLCPGR PCIKCDQFVT DALKTFENTY LNDHLPHDIH
60 70 80 90 100
KNVMRMVNHE VSSFGVVTSA EDSYLGAVDE NTLEQATWSF LKDLKRITDS
110 120 130 140 150
DLKGELFIKE LLWMLRHQKD IFNNLARQFQ KEVLCPNKCG VMSQTLIWCL
160 170 180 190 200
KCEKQLHICR KSLDCGERHI EVHRSEDLVL DCLLSWHRAS KGLTDYSFYR
210 220 230 240 250
VWENSSETLI AKGKEPYLTK SMVGPEDAGN YRCVLDTINQ GHATVIRYDV
260 270 280 290 300
TVLPPKHSEE NQPPNIITQE EHETPVHVTP QTPPGQEPES ELYPELHPEL
310 320 330 340 350
YPELIPTVAQ NPEKKMKTRL LILLTLGFVV LVASIIISVL HFRKVSAKLK
360 370 380 390
NASDEVKPTA SGSKSDQSLS QQMGLKKASQ ADFNSDYSGD KSEATEN
Length:397
Mass (Da):44,885
Last modified:June 1, 2001 - v1
Checksum:i70D10D480B78F5C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195681 mRNA. Translation: BAD91011.1.
AK006830 mRNA. Translation: BAB24761.1.
CCDSiCCDS21255.1.
RefSeqiNP_001018013.1. NM_001018013.1.
UniGeneiMm.380445.

Genome annotation databases

EnsembliENSMUST00000033100; ENSMUSP00000033100; ENSMUSG00000064158.
GeneIDi73456.
KEGGimmu:73456.
UCSCiuc009gwh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195681 mRNA. Translation: BAD91011.1.
AK006830 mRNA. Translation: BAB24761.1.
CCDSiCCDS21255.1.
RefSeqiNP_001018013.1. NM_001018013.1.
UniGeneiMm.380445.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B5KX-ray2.50A22-257[»]
ProteinModelPortaliQ9D9J7.
SMRiQ9D9J7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033100.

PTM databases

iPTMnetiQ9D9J7.
PhosphoSitePlusiQ9D9J7.

Proteomic databases

MaxQBiQ9D9J7.
PaxDbiQ9D9J7.
PRIDEiQ9D9J7.

Protocols and materials databases

DNASUi73456.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033100; ENSMUSP00000033100; ENSMUSG00000064158.
GeneIDi73456.
KEGGimmu:73456.
UCSCiuc009gwh.1. mouse.

Organism-specific databases

CTDi284359.
MGIiMGI:1920706. Izumo1.

Phylogenomic databases

eggNOGiENOG410IHEV. Eukaryota.
ENOG410Z8SJ. LUCA.
GeneTreeiENSGT00390000015014.
HOGENOMiHOG000113123.
HOVERGENiHBG081813.
InParanoidiQ9D9J7.
OMAiKEVHACR.
OrthoDBiEOG091G0AHL.
PhylomeDBiQ9D9J7.
TreeFamiTF338356.

Enzyme and pathway databases

ReactomeiR-MMU-1300652. Sperm:Oocyte Membrane Binding.

Miscellaneous databases

PROiQ9D9J7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064158.
CleanExiMM_IZUMO1.
GenevisibleiQ9D9J7. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR029389. IZUMO.
IPR032699. Izumo-Ig.
IPR032700. IZUMO1.
[Graphical view]
PANTHERiPTHR35540:SF1. PTHR35540:SF1. 1 hit.
PfamiPF15005. IZUMO. 1 hit.
PF16706. Izumo-Ig. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIZUM1_MOUSE
AccessioniPrimary (citable) accession number: Q9D9J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Izumo is the name of a Japanese shrine to marriage.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.