Q9D9J7 (IZUM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Izumo sperm-egg fusion protein 1

Alternative name(s):
Oocyte binding/fusion factor
Short name=OBF
Sperm-specific protein izumo

Gene names

Name:

Izumo1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the fertilization process. May be involved in the fusion of the sperm with the egg. Ref.1
Subunit structure	Monomer, homodimer and homooligomer. Ref.4
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction. Ref.1 Ref.4
Post-translational modification	N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm. Ref.3 Phosphorylated. Ref.4
Disruption phenotype	Mice are healthy but the males are sterile. They produce morphologically normal sperm that can bind to and penetrate the zona pellucida but that are incapable of fusing with eggs. Ref.1
Miscellaneous	Izumo is the name of a Japanese shrine to marriage.
Sequence similarities	Belongs to the Izumo family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred by curator Ref.1. Source: HGNC fusion of sperm to egg plasma membrane Inferred from direct assay Ref.1. Source: HGNC
Cellular_component	acrosomal membrane Inferred from direct assay PubMed 19144954PubMed 20375058. Source: MGI integral to membrane Inferred from direct assay Ref.1. Source: HGNC
Molecular_function	protein homodimerization activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 397	376	Izumo sperm-egg fusion protein 1		PRO_0000045483
Regions
	Topological domain	22 – 319	298	Extracellular Potential
	Transmembrane	320 – 340	21	Helical; Potential
	Topological domain	341 – 397	57	Cytoplasmic Potential
	Domain	167 – 251	85	Ig-like C2-type
Amino acid modifications
	Glycosylation	204	1	N-linked (GlcNAc...) Ref.3
	Disulfide bond	182 ↔ 233		By similarity
Experimental info
	Mutagenesis	204	1	N → Q: Almost no change in fusion-facilitating activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9D9J7 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 70D10D480B78F5C5	FASTA	397	44,885
	10 20 30 40 50 60 MGPHFTLLLA ALANCLCPGR PCIKCDQFVT DALKTFENTY LNDHLPHDIH KNVMRMVNHE 70 80 90 100 110 120 VSSFGVVTSA EDSYLGAVDE NTLEQATWSF LKDLKRITDS DLKGELFIKE LLWMLRHQKD 130 140 150 160 170 180 IFNNLARQFQ KEVLCPNKCG VMSQTLIWCL KCEKQLHICR KSLDCGERHI EVHRSEDLVL 190 200 210 220 230 240 DCLLSWHRAS KGLTDYSFYR VWENSSETLI AKGKEPYLTK SMVGPEDAGN YRCVLDTINQ 250 260 270 280 290 300 GHATVIRYDV TVLPPKHSEE NQPPNIITQE EHETPVHVTP QTPPGQEPES ELYPELHPEL 310 320 330 340 350 360 YPELIPTVAQ NPEKKMKTRL LILLTLGFVV LVASIIISVL HFRKVSAKLK NASDEVKPTA 370 380 390 SGSKSDQSLS QQMGLKKASQ ADFNSDYSGD KSEATEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs." Inoue N., Ikawa M., Isotani A., Okabe M. Nature 434:234-238(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Testis.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[3]	"Putative sperm fusion protein IZUMO and the role of N-glycosylation." Inoue N., Ikawa M., Okabe M. Biochem. Biophys. Res. Commun. 377:910-914(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-204, MUTAGENESIS OF ASN-204.
[4]	"Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm." Ellerman D.A., Pei J., Gupta S., Snell W.J., Myles D., Primakoff P. Mol. Reprod. Dev. 76:1188-1199(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY, PHOSPHORYLATION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB195681 mRNA. Translation: BAD91011.1. AK006830 mRNA. Translation: BAB24761.1.
IPI	IPI00112774.
RefSeq	NP_001018013.1. NM_001018013.1.
UniGene	Mm.380445.
3D structure databases
ProteinModelPortal	Q9D9J7.
SMR	Q9D9J7. Positions 166-252.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000033100.
PTM databases
PhosphoSite	Q9D9J7.
Proteomic databases
PaxDb	Q9D9J7.
PRIDE	Q9D9J7.
Protocols and materials databases
DNASU	73456.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033100; ENSMUSP00000033100; ENSMUSG00000064158.
GeneID	73456.
KEGG	mmu:73456.
UCSC	uc009gwh.1. mouse.
Organism-specific databases
CTD	284359.
MGI	MGI:1920706. Izumo1.
Phylogenomic databases
eggNOG	NOG41344.
GeneTree	ENSGT00390000015014.
HOGENOM	HOG000113123.
HOVERGEN	HBG081813.
InParanoid	Q9D9J7.
OMA	YRVWGNN.
OrthoDB	EOG4DJJWS.
Gene expression databases
Bgee	Q9D9J7.
CleanEx	MM_IZUMO1.
Genevestigator	Q9D9J7.
GermOnline	ENSMUSG00000064158. Mus musculus.
Family and domain databases
InterPro	IPR003599. Ig_sub. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	338309.
SOURCE	Search...

Entry information

Entry name

IZUM1_MOUSE

Accession

Primary (citable) accession number: Q9D9J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program