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Protein

CCR4-NOT transcription complex subunit 8

Gene

Cnot8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.1 Publication

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Divalent metal cation 1; catalyticBy similarity1
Metal bindingi40Divalent metal cation 2; catalyticBy similarity1
Metal bindingi42Divalent metal cation 2; catalyticBy similarity1
Metal bindingi161Divalent metal cation 1; catalyticBy similarity1
Metal bindingi230Divalent metal cation 2; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease, Repressor

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-429947. Deadenylation of mRNA.
R-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 8 (EC:3.1.13.4)
Alternative name(s):
CCR4-associated factor 8
Gene namesi
Name:Cnot8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1916375. Cnot8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128471 – 292CCR4-NOT transcription complex subunit 8Add BLAST292

Proteomic databases

EPDiQ9D8X5.
MaxQBiQ9D8X5.
PaxDbiQ9D8X5.
PeptideAtlasiQ9D8X5.
PRIDEiQ9D8X5.

PTM databases

iPTMnetiQ9D8X5.
PhosphoSitePlusiQ9D8X5.

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000020515.
CleanExiMM_CNOT8.
ExpressionAtlasiQ9D8X5. baseline and differential.
GenevisibleiQ9D8X5. MM.

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8. In the complex interacts directly with CNOT1. Interacts with BTG1, BTG2 and TOB1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi213244. 4 interactors.
DIPiDIP-61988N.
STRINGi10090.ENSMUSP00000020822.

Structurei

3D structure databases

ProteinModelPortaliQ9D8X5.
SMRiQ9D8X5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CAF1 family.Curated

Phylogenomic databases

eggNOGiKOG0304. Eukaryota.
COG5228. LUCA.
GeneTreeiENSGT00390000000080.
HOGENOMiHOG000173077.
HOVERGENiHBG051044.
InParanoidiQ9D8X5.
KOiK12581.
OMAiKSGIDFQ.
OrthoDBiEOG091G0M48.
PhylomeDBiQ9D8X5.
TreeFamiTF314185.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR027212. Cnot8.
IPR006941. RNase_CAF1.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10797:SF1. PTHR10797:SF1. 1 hit.
PfamiPF04857. CAF1. 2 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D8X5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAALVENSQ VICEVWASNL EEEMRKIREI VLSYSYIAMD TEFPGVVVRP
60 70 80 90 100
IGEFRSSIDY QYQLLRCNVD LLKIIQLGLT FTNEKGEYPS GINTWQFNFK
110 120 130 140 150
FNLTEDMYSQ DSIDLLANSG LQFQKHEEEG IDTLHFAELL MTSGVVLCDN
160 170 180 190 200
VKWLSFHSGY DFGYMVKLLT DSRLPEEEHE FFHILNLFFP SIYDVKYLMK
210 220 230 240 250
SCKNLKGGLQ EVADQLDLQR IGRQHQAGSD SLLTGMAFFR MKELFFEDSI
260 270 280 290
DDAKYCGRLY GLGTGVAQKQ NEDVDCAQEK MSILAMINNM QQ
Length:292
Mass (Da):33,574
Last modified:June 1, 2001 - v1
Checksum:i9CB57A966E51A91B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007581 mRNA. Translation: BAB25119.1.
AK075727 mRNA. Translation: BAC35913.1.
AK150757 mRNA. Translation: BAE29826.1.
AK153907 mRNA. Translation: BAE32248.1.
BC004040 mRNA. Translation: AAH04040.1.
CCDSiCCDS24722.1.
RefSeqiNP_081225.1. NM_026949.3.
XP_006534165.1. XM_006534102.1.
XP_006534166.1. XM_006534103.3.
XP_006534167.1. XM_006534104.1.
XP_006534168.1. XM_006534105.1.
XP_006534169.1. XM_006534106.3.
UniGeneiMm.28910.

Genome annotation databases

EnsembliENSMUST00000020822; ENSMUSP00000020822; ENSMUSG00000020515.
ENSMUST00000108843; ENSMUSP00000104471; ENSMUSG00000020515.
GeneIDi69125.
KEGGimmu:69125.
UCSCiuc007jaj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007581 mRNA. Translation: BAB25119.1.
AK075727 mRNA. Translation: BAC35913.1.
AK150757 mRNA. Translation: BAE29826.1.
AK153907 mRNA. Translation: BAE32248.1.
BC004040 mRNA. Translation: AAH04040.1.
CCDSiCCDS24722.1.
RefSeqiNP_081225.1. NM_026949.3.
XP_006534165.1. XM_006534102.1.
XP_006534166.1. XM_006534103.3.
XP_006534167.1. XM_006534104.1.
XP_006534168.1. XM_006534105.1.
XP_006534169.1. XM_006534106.3.
UniGeneiMm.28910.

3D structure databases

ProteinModelPortaliQ9D8X5.
SMRiQ9D8X5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213244. 4 interactors.
DIPiDIP-61988N.
STRINGi10090.ENSMUSP00000020822.

PTM databases

iPTMnetiQ9D8X5.
PhosphoSitePlusiQ9D8X5.

Proteomic databases

EPDiQ9D8X5.
MaxQBiQ9D8X5.
PaxDbiQ9D8X5.
PeptideAtlasiQ9D8X5.
PRIDEiQ9D8X5.

Protocols and materials databases

DNASUi69125.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020822; ENSMUSP00000020822; ENSMUSG00000020515.
ENSMUST00000108843; ENSMUSP00000104471; ENSMUSG00000020515.
GeneIDi69125.
KEGGimmu:69125.
UCSCiuc007jaj.1. mouse.

Organism-specific databases

CTDi9337.
MGIiMGI:1916375. Cnot8.

Phylogenomic databases

eggNOGiKOG0304. Eukaryota.
COG5228. LUCA.
GeneTreeiENSGT00390000000080.
HOGENOMiHOG000173077.
HOVERGENiHBG051044.
InParanoidiQ9D8X5.
KOiK12581.
OMAiKSGIDFQ.
OrthoDBiEOG091G0M48.
PhylomeDBiQ9D8X5.
TreeFamiTF314185.

Enzyme and pathway databases

ReactomeiR-MMU-429947. Deadenylation of mRNA.
R-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Miscellaneous databases

ChiTaRSiCnot8. mouse.
PROiQ9D8X5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020515.
CleanExiMM_CNOT8.
ExpressionAtlasiQ9D8X5. baseline and differential.
GenevisibleiQ9D8X5. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR027212. Cnot8.
IPR006941. RNase_CAF1.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10797:SF1. PTHR10797:SF1. 1 hit.
PfamiPF04857. CAF1. 2 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT8_MOUSE
AccessioniPrimary (citable) accession number: Q9D8X5
Secondary accession number(s): Q3U532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.