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Protein

CCR4-NOT transcription complex subunit 8

Gene

Cnot8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.1 Publication

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Divalent metal cation 1; catalyticBy similarity1
Metal bindingi40Divalent metal cation 2; catalyticBy similarity1
Metal bindingi42Divalent metal cation 2; catalyticBy similarity1
Metal bindingi161Divalent metal cation 1; catalyticBy similarity1
Metal bindingi230Divalent metal cation 2; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, Repressor, RNA-binding
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 8 (EC:3.1.13.4)
Alternative name(s):
CCR4-associated factor 8
Gene namesi
Name:Cnot8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1916375 Cnot8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128471 – 292CCR4-NOT transcription complex subunit 8Add BLAST292

Proteomic databases

EPDiQ9D8X5
MaxQBiQ9D8X5
PaxDbiQ9D8X5
PeptideAtlasiQ9D8X5
PRIDEiQ9D8X5

PTM databases

iPTMnetiQ9D8X5
PhosphoSitePlusiQ9D8X5

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000020515
CleanExiMM_CNOT8
ExpressionAtlasiQ9D8X5 baseline and differential
GenevisibleiQ9D8X5 MM

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8. In the complex interacts directly with CNOT1. Interacts with BTG1, BTG2 and TOB1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi213244, 4 interactors
DIPiDIP-61988N
IntActiQ9D8X5, 1 interactor
STRINGi10090.ENSMUSP00000020822

Structurei

3D structure databases

ProteinModelPortaliQ9D8X5
SMRiQ9D8X5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CAF1 family.Curated

Phylogenomic databases

eggNOGiKOG0304 Eukaryota
COG5228 LUCA
GeneTreeiENSGT00390000000080
HOGENOMiHOG000173077
HOVERGENiHBG051044
InParanoidiQ9D8X5
KOiK12581
OMAiHDFFQIL
OrthoDBiEOG091G0M48
PhylomeDBiQ9D8X5
TreeFamiTF314185

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR027212 Cnot8
IPR006941 RNase_CAF1
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10797:SF1 PTHR10797:SF1, 1 hit
PfamiView protein in Pfam
PF04857 CAF1, 2 hits
SUPFAMiSSF53098 SSF53098, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D8X5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAALVENSQ VICEVWASNL EEEMRKIREI VLSYSYIAMD TEFPGVVVRP
60 70 80 90 100
IGEFRSSIDY QYQLLRCNVD LLKIIQLGLT FTNEKGEYPS GINTWQFNFK
110 120 130 140 150
FNLTEDMYSQ DSIDLLANSG LQFQKHEEEG IDTLHFAELL MTSGVVLCDN
160 170 180 190 200
VKWLSFHSGY DFGYMVKLLT DSRLPEEEHE FFHILNLFFP SIYDVKYLMK
210 220 230 240 250
SCKNLKGGLQ EVADQLDLQR IGRQHQAGSD SLLTGMAFFR MKELFFEDSI
260 270 280 290
DDAKYCGRLY GLGTGVAQKQ NEDVDCAQEK MSILAMINNM QQ
Length:292
Mass (Da):33,574
Last modified:June 1, 2001 - v1
Checksum:i9CB57A966E51A91B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007581 mRNA Translation: BAB25119.1
AK075727 mRNA Translation: BAC35913.1
AK150757 mRNA Translation: BAE29826.1
AK153907 mRNA Translation: BAE32248.1
BC004040 mRNA Translation: AAH04040.1
CCDSiCCDS24722.1
RefSeqiNP_081225.1, NM_026949.3
XP_006534165.1, XM_006534102.1
XP_006534166.1, XM_006534103.3
XP_006534167.1, XM_006534104.1
XP_006534168.1, XM_006534105.1
XP_006534169.1, XM_006534106.3
UniGeneiMm.28910

Genome annotation databases

EnsembliENSMUST00000020822; ENSMUSP00000020822; ENSMUSG00000020515
ENSMUST00000108843; ENSMUSP00000104471; ENSMUSG00000020515
GeneIDi69125
KEGGimmu:69125
UCSCiuc007jaj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCNOT8_MOUSE
AccessioniPrimary (citable) accession number: Q9D8X5
Secondary accession number(s): Q3U532
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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