Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SRA stem-loop-interacting RNA-binding protein, mitochondrial

Gene

Slirp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • mitochondrion morphogenesis Source: MGI
  • negative regulation of mitochondrial RNA catabolic process Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • single fertilization Source: MGI
  • spermatid development Source: MGI
  • sperm motility Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SRA stem-loop-interacting RNA-binding protein, mitochondrial
Gene namesi
Name:Slirp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1916394. Slirp.

Subcellular locationi

  • Mitochondrion By similarity
  • Nucleus By similarity

  • Note: Predominantly mitochondrial. Some fraction is nuclear. In the nucleus, it is recruited to nuclear receptor target promoters (By similarity).By similarity

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • cytoplasm Source: MGI
  • intracellular ribonucleoprotein complex Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: MGI
  • sperm flagellum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 112SRA stem-loop-interacting RNA-binding protein, mitochondrialPRO_0000247052
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei104 – 1041PhosphothreonineCombined sources
Modified residuei105 – 1051PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D8T7.
MaxQBiQ9D8T7.
PaxDbiQ9D8T7.
PRIDEiQ9D8T7.

PTM databases

iPTMnetiQ9D8T7.
PhosphoSiteiQ9D8T7.
SwissPalmiQ9D8T7.

Expressioni

Gene expression databases

BgeeiQ9D8T7.
CleanExiMM_1810035L17RIK.
ExpressionAtlasiQ9D8T7. baseline and differential.
GenevisibleiQ9D8T7. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9D8T7. 3 interactions.
MINTiMINT-4127671.
STRINGi10090.ENSMUSP00000125341.

Structurei

3D structure databases

ProteinModelPortaliQ9D8T7.
SMRiQ9D8T7. Positions 22-112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9880RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00390000008624.
HOGENOMiHOG000276232.
HOVERGENiHBG079935.
InParanoidiQ9D8T7.
OMAiKETGFHK.
OrthoDBiEOG7WT44N.
PhylomeDBiQ9D8T7.
TreeFamiTF319527.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D8T7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASAIKGLS ALRSSTGRPI AFVRKIPWTA AASELREHFA QFGHVRRCTV
60 70 80 90 100
PFDKETGFHR GMGWVQFSSQ EELQNALQQE HHIIDGVKIH VQAQRAKALH
110
GAQTSDEERF LR
Length:112
Mass (Da):12,605
Last modified:July 25, 2006 - v2
Checksum:i3A03B372E1DA05DB
GO
Isoform 2 (identifier: Q9D8T7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-112: IHVQAQRAKALHGAQTSDEERFLR → VSISDVCSTSL

Note: No experimental confirmation available.
Show »
Length:99
Mass (Da):10,953
Checksum:i0A520C6F6F04BA1B
GO

Sequence cautioni

The sequence CAC34586.1 differs from that shown. Reason: Frameshift at position 4. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511P → H in BAB25197 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei89 – 11224IHVQA…ERFLR → VSISDVCSTSL in isoform 2. 1 PublicationVSP_019907Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293625 mRNA. Translation: CAC34586.1. Frameshift.
AK007699 mRNA. Translation: BAB25197.1.
BC100567 mRNA. Translation: AAI00568.1.
CCDSiCCDS36503.1. [Q9D8T7-1]
RefSeqiNP_081234.2. NM_026958.3. [Q9D8T7-1]
UniGeneiMm.355701.

Genome annotation databases

EnsembliENSMUST00000160488; ENSMUSP00000124174; ENSMUSG00000021040. [Q9D8T7-2]
ENSMUST00000161023; ENSMUSP00000125341; ENSMUSG00000021040. [Q9D8T7-1]
GeneIDi380773.
KEGGimmu:380773.
UCSCiuc007ojd.2. mouse. [Q9D8T7-1]
uc007oje.2. mouse. [Q9D8T7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293625 mRNA. Translation: CAC34586.1. Frameshift.
AK007699 mRNA. Translation: BAB25197.1.
BC100567 mRNA. Translation: AAI00568.1.
CCDSiCCDS36503.1. [Q9D8T7-1]
RefSeqiNP_081234.2. NM_026958.3. [Q9D8T7-1]
UniGeneiMm.355701.

3D structure databases

ProteinModelPortaliQ9D8T7.
SMRiQ9D8T7. Positions 22-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D8T7. 3 interactions.
MINTiMINT-4127671.
STRINGi10090.ENSMUSP00000125341.

PTM databases

iPTMnetiQ9D8T7.
PhosphoSiteiQ9D8T7.
SwissPalmiQ9D8T7.

Proteomic databases

EPDiQ9D8T7.
MaxQBiQ9D8T7.
PaxDbiQ9D8T7.
PRIDEiQ9D8T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160488; ENSMUSP00000124174; ENSMUSG00000021040. [Q9D8T7-2]
ENSMUST00000161023; ENSMUSP00000125341; ENSMUSG00000021040. [Q9D8T7-1]
GeneIDi380773.
KEGGimmu:380773.
UCSCiuc007ojd.2. mouse. [Q9D8T7-1]
uc007oje.2. mouse. [Q9D8T7-2]

Organism-specific databases

CTDi81892.
MGIiMGI:1916394. Slirp.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00390000008624.
HOGENOMiHOG000276232.
HOVERGENiHBG079935.
InParanoidiQ9D8T7.
OMAiKETGFHK.
OrthoDBiEOG7WT44N.
PhylomeDBiQ9D8T7.
TreeFamiTF319527.

Miscellaneous databases

ChiTaRSiSlirp. mouse.
PROiQ9D8T7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D8T7.
CleanExiMM_1810035L17RIK.
ExpressionAtlasiQ9D8T7. baseline and differential.
GenevisibleiQ9D8T7. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Full length sequencing of some human and murine muscular transcripts (Telethon Italy project B41)."
    Ievolella C., Zara I., Millino C., Faulkner G., Lanfranchi G.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Skeletal muscle.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-112 (ISOFORM 1).
    Tissue: Pancreas.
  4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-104 AND SER-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Pancreas and Testis.

Entry informationi

Entry nameiSLIRP_MOUSE
AccessioniPrimary (citable) accession number: Q9D8T7
Secondary accession number(s): Q497G4, Q99JH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: June 8, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.