Q9D8M7 (PHF10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PHD finger protein 10

Alternative name(s):
BRG1-associated factor 45a
Short name=BAF45a

Gene names

Name:	Phf10
Synonyms:	Baf45a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Ref.4
Subunit structure	Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Interacts with ACTL6A/BAF53A, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A and PBRM1/BAF180. Ref.4
Subcellular location	Nucleus By similarity.
Tissue specificity	Widely expressed. Expressed selectively in neural stem and progenitor cells (at protein level). Ref.4
Developmental stage	Expressed in neural cells at 10.5-11.5 dpc. At 10.5 to 16.5 dpc, in the developing spinal cord, specifically expressed in proliferating neural progenitors of the ventricular zone. In the developing forebrain and cerebellar primordium, expression is restricted to proliferating neuroepithelial progenitors and cerebellar granule precursors. Ref.4
Sequence similarities	Belongs to the SAYP family. Contains 2 PHD-type zinc fingers.
Caution	It is uncertain whether Met-1 or Met-88 is the initiator.
Sequence caution	The sequence AAH02206.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB25323.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nervous system development Inferred from mutant phenotype Ref.4. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	npBAF complex Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D8M7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9D8M7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     108-109: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	PHD finger protein 10		PRO_0000059298
Regions
	Zinc finger	378 – 435	58	PHD-type 1; degenerate
	Zinc finger	437 – 480	44	PHD-type 2; degenerate
	Region	88 – 294	207	SAY
	Region	88 – 184	97	Essential to induce neural progenitor proliferation
	Region	291 – 333	43	Essential to induce neural progenitor proliferation
Amino acid modifications
	Modified residue	11	1	Phosphoserine By similarity
	Modified residue	35	1	Phosphoserine By similarity
	Modified residue	49	1	Phosphoserine By similarity
	Modified residue	269	1	Phosphoserine By similarity
	Modified residue	296	1	Phosphoserine By similarity
	Modified residue	300	1	Phosphoserine By similarity
	Modified residue	326	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	108 – 109	2	Missing in isoform 2.		VSP_013441
Experimental info
	Sequence conflict	2	1	T → A in BAB25323. Ref.1
	Sequence conflict	10	1	P → L in BAB25323. Ref.1
	Sequence conflict	115	1	S → Y in BAB25323. Ref.1
	Sequence conflict	349	1	M → V in BAB25323. Ref.1
	Sequence conflict	416	1	M → V in BAB25323. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: A1F27CB9F616A271	FASTA	497	55,840
	10 20 30 40 50 60 MTAAGPGAAP SPGRCDSDPA SPGAQSPKDD NEDNSNDGTH PCKRRRMGSG DSSRSCETSS 70 80 90 100 110 120 QDLSFSYYPA ENLIEYKWPP DETGEYYMLQ EQVSEYLGVT SFKRKYPDLE RRDLSHKEKL 130 140 150 160 170 180 YLRELNVITE TQCTLGLTAL RSDEVIDLMI KEYPAKHAEY SVILQEKERQ RITDHYKEYS 190 200 210 220 230 240 QMQQQSTQKV EASKVPEYIK KAAKKAAEFN SNLNRERMEE RRAYFDLQTH VIQVPQGKYK 250 260 270 280 290 300 VLPTDRTKVS SYPVALIPGQ FQEYYKRYSP DELRYLPLNT ALYEPPLDPE LPALDSDGDS 310 320 330 340 350 360 DDGEDGGGDE KRKNKGTSDS SSGNVSEGDS PPDSQEDTFH GRQKSKDKMA TPRKDGSKRS 370 380 390 400 410 420 VLSKSAPGYK PKVIPNALCG ICLKGKESNK KGKAESLIHC SQCDNSGHPS CLDMTMELVS 430 440 450 460 470 480 MIKTYPWQCM ECKTCIICGQ PHHEEEMMFC DVCDRGYHTF CVGLGAIPSG RWICDCCQRA 490 PPTPRKVGRR GKNSKEG
	Isoform 2 [UniParc]. Checksum: C4D10352EBBE5FD6	FASTA	495	55,611
	10 20 30 40 50 60 MTAAGPGAAP SPGRCDSDPA SPGAQSPKDD NEDNSNDGTH PCKRRRMGSG DSSRSCETSS 70 80 90 100 110 120 QDLSFSYYPA ENLIEYKWPP DETGEYYMLQ EQVSEYLGVT SFKRKYPERR DLSHKEKLYL 130 140 150 160 170 180 RELNVITETQ CTLGLTALRS DEVIDLMIKE YPAKHAEYSV ILQEKERQRI TDHYKEYSQM 190 200 210 220 230 240 QQQSTQKVEA SKVPEYIKKA AKKAAEFNSN LNRERMEERR AYFDLQTHVI QVPQGKYKVL 250 260 270 280 290 300 PTDRTKVSSY PVALIPGQFQ EYYKRYSPDE LRYLPLNTAL YEPPLDPELP ALDSDGDSDD 310 320 330 340 350 360 GEDGGGDEKR KNKGTSDSSS GNVSEGDSPP DSQEDTFHGR QKSKDKMATP RKDGSKRSVL 370 380 390 400 410 420 SKSAPGYKPK VIPNALCGIC LKGKESNKKG KAESLIHCSQ CDNSGHPSCL DMTMELVSMI 430 440 450 460 470 480 KTYPWQCMEC KTCIICGQPH HEEEMMFCDV CDRGYHTFCV GLGAIPSGRW ICDCCQRAPP 490 TPRKVGRRGK NSKEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Pancreas.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 66-497 (ISOFORM 2). Strain: FVB/N. Tissue: Mammary tumor.
[4]	"An essential switch in subunit composition of a chromatin remodeling complex during neural development." Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R. Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, FUNCTION OF THE NBAF AND NPBAF COMPLEXES, IDENTIFICATION IN THE NPBAF COMPLEX, INTERACTION WITH ACTL6A; SMARCA2; SMARCA4 AND PBRM1, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK007873 mRNA. Translation: BAB25323.1. Different initiation. AC154411 Genomic DNA. No translation available. AC182749 Genomic DNA. No translation available. BC002206 mRNA. Translation: AAH02206.1. Different initiation.
IPI	IPI00318829. IPI00556703.
RefSeq	NP_077212.3. NM_024250.4.
UniGene	Mm.440085.
3D structure databases
ProteinModelPortal	Q9D8M7.
SMR	Q9D8M7. Positions 375-478.
ModBase	Search...
PTM databases
PhosphoSite	Q9D8M7.
Proteomic databases
PRIDE	Q9D8M7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	72057.
KEGG	mmu:72057.
UCSC	uc008anj.2. mouse. uc008ank.2. mouse.
Organism-specific databases
CTD	55274.
MGI	MGI:1919307. Phf10.
Phylogenomic databases
eggNOG	NOG279558.
HOGENOM	HOG000286026.
HOVERGEN	HBG053581.
InParanoid	Q9D8M7.
OrthoDB	EOG4BCDNG.
Gene expression databases
ArrayExpress	Q9D8M7.
Bgee	Q9D8M7.
CleanEx	MM_PHF10.
Genevestigator	Q9D8M7.
GermOnline	ENSMUSG00000023883. Mus musculus.
Family and domain databases
Gene3D	3.30.40.10. 2 hits.
InterPro	IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF00628. PHD. 1 hit. [Graphical view]
SMART	SM00249. PHD. 2 hits. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 2 hits.
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	335336.
SOURCE	Search...

Entry information

Entry name

PHF10_MOUSE

Accession

Primary (citable) accession number: Q9D8M7
Secondary accession number(s): E9QLI2, Q99LV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 92 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program