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Q9D8I1

- MZB1_MOUSE

UniProt

Q9D8I1 - MZB1_MOUSE

Protein

Marginal zone B- and B1-cell-specific protein

Gene

Mzb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Helps to diversify peripheral B-cell functions by regulating Ca2+ stores, antibody secretion and integrin activation.
    Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. integrin activation Source: UniProtKB
    2. negative regulation of glucose import in response to insulin stimulus Source: UniProtKB
    3. positive regulation of cell proliferation Source: UniProtKB
    4. positive regulation of immunoglobulin biosynthetic process Source: UniProtKB
    5. regulation of B cell proliferation Source: UniProtKB
    6. regulation of cell proliferation Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Marginal zone B- and B1-cell-specific protein
    Alternative name(s):
    Plasma cell-induced resident endoplasmic reticulum protein
    Short name:
    Plasma cell-induced resident ER protein
    Short name:
    pERp1
    Proapoptotic caspase adapter protein
    Gene namesi
    Name:Mzb1
    Synonyms:Pacap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1917066. Mzb1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum chaperone complex Source: UniProtKB
    2. endoplasmic reticulum lumen Source: UniProtKB
    3. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi49 – 491C → A: Reduced electrophoretic mobility; when associated with A-52. 1 Publication
    Mutagenesisi52 – 521C → A: Reduced electrophoretic mobility; when associated with A-49. 1 Publication
    Mutagenesisi94 – 941C → A: Small loss of electrophoretic mobility. 1 Publication
    Mutagenesisi142 – 1421C → A: Small loss of electrophoretic mobility. 1 Publication
    Mutagenesisi170 – 1701C → A: Reduced electrophoretic mobility; when associated with A-177. 1 Publication
    Mutagenesisi177 – 1771C → A: Reduced electrophoretic mobility; when associated with A-170. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 188168Marginal zone B- and B1-cell-specific proteinPRO_0000318741Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 1771 Publication
    Disulfide bondi52 ↔ 1701 Publication
    Disulfide bondi94 ↔ 1421 Publication

    Post-translational modificationi

    Forms an interchain disulfide bond with IgM monomers.

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ9D8I1.
    PaxDbiQ9D8I1.
    PRIDEiQ9D8I1.

    PTM databases

    PhosphoSiteiQ9D8I1.

    Expressioni

    Tissue specificityi

    Expressed predominantly in the spleen and lymph nodes. Abundantly expressed in marginal zone B and B1 cells. High expression in mesenteric adipose tissue (MAT). Expressed also in pancreas, perigonadal adipose tissue (PAT), uterus, subcutaneous adipose tissue, heart, muscle, ovary and liver. Very low expression is detected in brown adipose tissue. In PAT, significantly higher expression in stromal-vascular cell than in adipocytes. Expressed in macrophage RAW 264.7 cell line. Down-regulated in For-knockout female MAT at 5 months (obese state) followed by steep up-regulation at 9 months (prediabetic condition) when mutants progress towards the metabolic syndrome.3 Publications

    Developmental stagei

    Up-regulated during plasma cell differentiation.2 Publications

    Gene expression databases

    BgeeiQ9D8I1.
    CleanExiMM_2010001M09RIK.
    GenevestigatoriQ9D8I1.

    Interactioni

    Subunit structurei

    Part of the ER chaperone complex, a multi-protein complex in the endoplasmic reticulum containing a large number of molecular chaperones which associates with unassembled incompletely folded immunoglobulin heavy chains. Interacts with HSP90B1 and PDIA3 in a calcium-dependent manner.2 Publications

    Protein-protein interaction databases

    DIPiDIP-48984N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D8I1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi185 – 1884Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the MZB1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG74024.
    GeneTreeiENSGT00390000002716.
    HOGENOMiHOG000068171.
    HOVERGENiHBG108230.
    InParanoidiQ9D8I1.
    OMAiGPEPRIS.
    OrthoDBiEOG7D2FFZ.
    PhylomeDBiQ9D8I1.
    TreeFamiTF329450.

    Family and domain databases

    InterProiIPR021852. DUF3456.
    [Graphical view]
    PfamiPF11938. DUF3456. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D8I1-1 [UniParc]FASTAAdd to Basket

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    MRLPLPLLLL FGCRAILGSA GDRVSLSASA PTLDDEEKYS AHMPAHLRCD    50
    ACRAVAFQMG QRLAKAEAKS HTPDASGLQE LSESTYTDVL DQTCSQNWQS 100
    YGVHEVNQMK RLTGPGLSKG PEPRISVMIS GGPWPNRLSK TCFHYLGEFG 150
    EDQIYEAYRQ GQANLEALLC GGTHGPCSQE ILAQREEL 188
    Length:188
    Mass (Da):20,580
    Last modified:February 26, 2008 - v2
    Checksum:i101640BE6E9B0A93
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61P → T in BAC40141. (PubMed:16141072)Curated
    Sequence conflicti11 – 111F → L in ACY74344. (PubMed:19805157)Curated
    Sequence conflicti11 – 111F → L in BAB25410. (PubMed:16141072)Curated
    Sequence conflicti31 – 311P → H in ACY74344. (PubMed:19805157)Curated
    Sequence conflicti31 – 311P → H in BAB25410. (PubMed:16141072)Curated
    Sequence conflicti136 – 1361N → S in BAB25410. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ477351 mRNA. Translation: ACY74344.1.
    AK008016 mRNA. Translation: BAB25410.1.
    AK088094 mRNA. Translation: BAC40141.1.
    BC030674 mRNA. Translation: AAH30674.1.
    BC064044 mRNA. Translation: AAH64044.1.
    CCDSiCCDS29145.1.
    RefSeqiNP_081498.2. NM_027222.3.
    UniGeneiMm.27252.

    Genome annotation databases

    EnsembliENSMUST00000025211; ENSMUSP00000025211; ENSMUSG00000024353.
    GeneIDi69816.
    KEGGimmu:69816.
    UCSCiuc008emm.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ477351 mRNA. Translation: ACY74344.1 .
    AK008016 mRNA. Translation: BAB25410.1 .
    AK088094 mRNA. Translation: BAC40141.1 .
    BC030674 mRNA. Translation: AAH30674.1 .
    BC064044 mRNA. Translation: AAH64044.1 .
    CCDSi CCDS29145.1.
    RefSeqi NP_081498.2. NM_027222.3.
    UniGenei Mm.27252.

    3D structure databases

    ProteinModelPortali Q9D8I1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48984N.

    PTM databases

    PhosphoSitei Q9D8I1.

    Proteomic databases

    MaxQBi Q9D8I1.
    PaxDbi Q9D8I1.
    PRIDEi Q9D8I1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025211 ; ENSMUSP00000025211 ; ENSMUSG00000024353 .
    GeneIDi 69816.
    KEGGi mmu:69816.
    UCSCi uc008emm.2. mouse.

    Organism-specific databases

    CTDi 51237.
    MGIi MGI:1917066. Mzb1.

    Phylogenomic databases

    eggNOGi NOG74024.
    GeneTreei ENSGT00390000002716.
    HOGENOMi HOG000068171.
    HOVERGENi HBG108230.
    InParanoidi Q9D8I1.
    OMAi GPEPRIS.
    OrthoDBi EOG7D2FFZ.
    PhylomeDBi Q9D8I1.
    TreeFami TF329450.

    Miscellaneous databases

    NextBioi 330410.
    PROi Q9D8I1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D8I1.
    CleanExi MM_2010001M09RIK.
    Genevestigatori Q9D8I1.

    Family and domain databases

    InterProi IPR021852. DUF3456.
    [Graphical view ]
    Pfami PF11938. DUF3456. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "pERp1 is significantly up-regulated during plasma cell differentiation and contributes to the oxidative folding of immunoglobulin."
      Shimizu Y., Meunier L., Hendershot L.M.
      Proc. Natl. Acad. Sci. U.S.A. 106:17013-17018(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION IN ER CHAPERONE COMPLEX, DEVELOPMENTAL STAGE, IDENTIFICATION BY MASS SPECTROMETRY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Small intestine and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    4. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISULFIDE BONDS, MUTAGENESIS OF CYS-49; CYS-52; CYS-94; CYS-142; CYS-170 AND CYS-177, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Mzb1 protein regulates calcium homeostasis, antibody secretion, and integrin activation in innate-like B cells."
      Flach H., Rosenbaum M., Duchniewicz M., Kim S., Zhang S.L., Cahalan M.D., Mittler G., Grosschedl R.
      Immunity 33:723-735(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH HSP90B1 AND PDIA3.
    6. "Novel hormone-regulated genes in visceral adipose tissue: cloning and identification of proinflammatory cytokine-like mouse and human MEDA-7: implications for obesity, insulin resistance and the metabolic syndrome."
      Zhang H., Chen X., Sairam M.R.
      Diabetologia 54:2368-2380(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.

    Entry informationi

    Entry nameiMZB1_MOUSE
    AccessioniPrimary (citable) accession number: Q9D8I1
    Secondary accession number(s): D2IYS1
    , Q6P3D3, Q8BU13, Q8K2M5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3