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Q9D8C3

- ALG13_MOUSE

UniProt

Q9D8C3 - ALG13_MOUSE

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Protein
Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13
Gene
Alg13, Glt28d1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Isoform 1: Possible multifunctional enzyme with both glycosyltransferase and deubiquitinase activities.
Isoform 2: May be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway (By. similarity).

Catalytic activityi

UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei233 – 2331For deubiquitinase activity By similarity
Active sitei236 – 2361Nucleophile; for deubiquitinase activity By similarity
Active sitei339 – 3391For deubiquitinase activity By similarity

GO - Molecular functioni

  1. N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. lipid glycosylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Protease, Thiol protease, Transferase

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 (EC:2.4.1.141, EC:3.4.19.12)
Alternative name(s):
Asparagine-linked glycosylation 13 homolog
Glycosyltransferase 28 domain-containing protein 1
UDP-N-acetylglucosamine transferase subunit ALG13 homolog
Gene namesi
Name:Alg13
Synonyms:Glt28d1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1914824. Alg13.

Subcellular locationi

Endoplasmic reticulum By similarity
Note: Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14 By similarity.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11661166Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13
PRO_0000254574Add
BLAST

Proteomic databases

MaxQBiQ9D8C3.
PaxDbiQ9D8C3.
PRIDEiQ9D8C3.

PTM databases

PhosphoSiteiQ9D8C3.

Expressioni

Gene expression databases

BgeeiQ9D8C3.
GenevestigatoriQ9D8C3.

Interactioni

Subunit structurei

Isoform 2 may interact with ALG14 By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042810.

Structurei

3D structure databases

ProteinModelPortaliQ9D8C3.
SMRiQ9D8C3. Positions 2-176, 215-343.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 346122OTU
Add
BLAST
Domaini486 – 54661Tudor
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 125125Glycosyltransferase activity By similarity
Add
BLAST
Regioni126 – 394269Deubiquitinase activity By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi875 – 1033159Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 1 OTU domain.
Contains 1 Tudor domain.

Phylogenomic databases

eggNOGiCOG5017.
GeneTreeiENSGT00510000047493.
HOGENOMiHOG000192807.
HOVERGENiHBG056051.
InParanoidiA3KGC8.
KOiK07432.
OMAiNERLMDN.
OrthoDBiEOG7NPFVV.
PhylomeDBiQ9D8C3.
TreeFamiTF313788.

Family and domain databases

InterProiIPR007235. Glyco_trans_28_C.
IPR003323. OTU.
IPR002999. Tudor.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9D8C3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKRAFVTVGT TSFDELVARV VANDCVQILE SLGYNHLVLQ VGRGTVVPKP     50
FRTESFTLDV YRYKDSLKED LQQADLVISH AGAGSCLESL EKGKPLVVVV 100
NEKLMNNHQF ELAKQLHKEG HLFYCTCRVL SCPAPVSLLL VLLGSAKILQ 150
QLPSATLSCF GYLPTQAPVL VATAYSYLHS VFSSFPPLST FLIIPCTMQK 200
GWKKYCGQKS LNEASMDEYL GSLGLFRKVV AKDASCLFRA ISEQLFHSQI 250
HHLQIRRACV SYMKENQQAF ESYVEGSFEK YLERLGDPKE SAGQLELKAL 300
SLIYNRDFII YRYPGKPPTQ VTDNGFEDKI ILCYSNNGHY DSVYSKEFQS 350
TAGICQAILY ELLYKDVFVV DEETLKTAVD LFRSGSRRNK HHALTASVEG 400
SSDQKSSTED RTEEAAACSS AASTPEGNKQ GTERQKVPES PSKMLFPYKV 450
LKALDPEIYR NVEFDAWLDS RKELQKSECV EYGGRYYFLG DKCQVCMESG 500
GKYYNAHIQE IDNDKSSVVV FIEEFAERHS IPLAHVRPVN QVALLPSWNA 550
IPIRNGRGYP TITGGYFPEI VMTDMNMKQR KKMFKKFRGK EIYMTMAYSR 600
GDPLVPSRIQ HSMHYGHDPL LYYSQTAGHI LSSQHFYPQH SSQRQGRGYG 650
MPRDSSHLIS KQNLPNPKVG FCSGSGRKCC QSYDNVSYRS RSFRRSHRQM 700
HCMNKGCQYG FAPENGVEET VTFYALEEGN ETAYSTLPNN GGPTTMVPAT 750
SGYCVARQGY NSCKPPLNSG DSNDHCDNGG YHGDYLYSSE QGYETSSVYT 800
TTVSTANLSL QDSGPSSVPQ DTVTSYNYPQ KVLENSAAIA VSWASHVPVP 850
VIPNCAGDNE ALRTSDISSQ NAIQPGFVPP PAQGSPAYLE PSAAGAAGAA 900
AAAAAAAAPV ATPVAAPLPL PPPLPPPPPA TLEAGDASGF PLPPPPPPPP 950
PPPPPYSYDP SGSDLPQDTK VLQYYFNLGL QCYHHNYWHP MVYMPHVQQQ 1000
LQPQPQPQPQ PQPQPQPQPQ PQPQQPQQQQ PPPQQQQQQQ EQVHGESYPD 1050
CTEQPLVDQS APQVYSDVVR EDGTQADVST NDTFPIADAV PLPHGAVYYP 1100
VMTDPYGSPL LGFDSYVPVA SDYSSIAMWH PVNAACGASA QIHGAMNPGP 1150
IGYMLLPNSP HYTPQN 1166
Length:1,166
Mass (Da):128,664
Last modified:June 15, 2010 - v2
Checksum:i5F045ACF188BBE8A
GO
Isoform 2 (identifier: Q9D8C3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-165: RVLSCPAPVS...TLSCFGYLPT → STLPGLLQSM...FLDKVVGLQK
     166-1166: Missing.

Show »
Length:165
Mass (Da):18,346
Checksum:i947DFE404D54FA8C
GO

Sequence cautioni

The sequence CAM45938.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAM45939.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei128 – 16538RVLSC…GYLPT → STLPGLLQSMDLSTLKCYPP GQPEKFSAFLDKVVGLQK in isoform 2.
VSP_039304Add
BLAST
Alternative sequencei166 – 11661001Missing in isoform 2.
VSP_039305Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q9D8C3-2)
Sequence conflicti149 – 1491Q → H in BAB29554. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK008162 mRNA. Translation: BAB25504.1.
AK014790 mRNA. Translation: BAB29554.1.
AK086546 mRNA. Translation: BAC39689.1.
AK089102 mRNA. Translation: BAC40750.1.
AL713978 Genomic DNA. Translation: CAM45936.1.
AL713978 Genomic DNA. Translation: CAM45938.1. Sequence problems.
AL713978 Genomic DNA. Translation: CAM45939.1. Sequence problems.
CH466610 Genomic DNA. Translation: EDL14725.1.
BC053004 mRNA. No translation available.
BC137692 mRNA. Translation: AAI37693.1.
CCDSiCCDS30456.1. [Q9D8C3-2]
RefSeqiNP_080523.2. NM_026247.3. [Q9D8C3-2]
UniGeneiMm.249084.
Mm.484110.

Genome annotation databases

EnsembliENSMUST00000070801; ENSMUSP00000068403; ENSMUSG00000041718. [Q9D8C3-2]
GeneIDi67574.
KEGGimmu:67574.
UCSCiuc009ums.2. mouse. [Q9D8C3-2]
uc009umu.2. mouse. [Q9D8C3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK008162 mRNA. Translation: BAB25504.1 .
AK014790 mRNA. Translation: BAB29554.1 .
AK086546 mRNA. Translation: BAC39689.1 .
AK089102 mRNA. Translation: BAC40750.1 .
AL713978 Genomic DNA. Translation: CAM45936.1 .
AL713978 Genomic DNA. Translation: CAM45938.1 . Sequence problems.
AL713978 Genomic DNA. Translation: CAM45939.1 . Sequence problems.
CH466610 Genomic DNA. Translation: EDL14725.1 .
BC053004 mRNA. No translation available.
BC137692 mRNA. Translation: AAI37693.1 .
CCDSi CCDS30456.1. [Q9D8C3-2 ]
RefSeqi NP_080523.2. NM_026247.3. [Q9D8C3-2 ]
UniGenei Mm.249084.
Mm.484110.

3D structure databases

ProteinModelPortali Q9D8C3.
SMRi Q9D8C3. Positions 2-176, 215-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000042810.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSitei Q9D8C3.

Proteomic databases

MaxQBi Q9D8C3.
PaxDbi Q9D8C3.
PRIDEi Q9D8C3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000070801 ; ENSMUSP00000068403 ; ENSMUSG00000041718 . [Q9D8C3-2 ]
GeneIDi 67574.
KEGGi mmu:67574.
UCSCi uc009ums.2. mouse. [Q9D8C3-2 ]
uc009umu.2. mouse. [Q9D8C3-1 ]

Organism-specific databases

CTDi 79868.
MGIi MGI:1914824. Alg13.

Phylogenomic databases

eggNOGi COG5017.
GeneTreei ENSGT00510000047493.
HOGENOMi HOG000192807.
HOVERGENi HBG056051.
InParanoidi A3KGC8.
KOi K07432.
OMAi NERLMDN.
OrthoDBi EOG7NPFVV.
PhylomeDBi Q9D8C3.
TreeFami TF313788.

Miscellaneous databases

NextBioi 324945.
PROi Q9D8C3.
SOURCEi Search...

Gene expression databases

Bgeei Q9D8C3.
Genevestigatori Q9D8C3.

Family and domain databases

InterProi IPR007235. Glyco_trans_28_C.
IPR003323. OTU.
IPR002999. Tudor.
[Graphical view ]
Pfami PF04101. Glyco_tran_28_C. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J and NOD.
    Tissue: Head, Small intestine and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Fetal brain.

Entry informationi

Entry nameiALG13_MOUSE
AccessioniPrimary (citable) accession number: Q9D8C3
Secondary accession number(s): A3KGC8
, A3KGD0, A3KGD1, Q8BUA4, Q9D5Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 15, 2010
Last modified: July 9, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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