Q9D8C3 (ALG13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine transferase subunit ALG13 homolog
EC=2.4.1.141

Alternative name(s):
Asparagine-linked glycosylation 13 homolog
Glycosyltransferase 28 domain-containing protein 1

Gene names

Name:	Alg13
Synonyms:	Glt28d1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1166 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Isoform 2 may be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway (By. similarity).
Catalytic activity	UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.
Subunit structure	Isoform 2 may interact with ALG14 By similarity.
Subcellular location	Endoplasmic reticulum By similarity. Note: Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14 By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 1 OTU domain. Contains 1 Tudor domain.
Sequence caution	The sequence CAM45938.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM45939.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9D8C3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9D8C3-2) The sequence of this isoform differs from the canonical sequence as follows:      128-165: RVLSCPAPVS...TLSCFGYLPT → STLPGLLQSM...FLDKVVGLQK      166-1166: Missing.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Sequence conflict	149	1	Q → H in BAB29554. Ref.1

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1166	1166	UDP-N-acetylglucosamine transferase subunit ALG13 homolog		PRO_0000254574
Regions
	Domain	225 – 346	122	OTU
	Domain	486 – 546	61	Tudor
	Compositional bias	875 – 1033	159	Pro-rich
Natural variations
	Alternative sequence	128 – 165	38	RVLSC…GYLPT → STLPGLLQSMDLSTLKCYPP GQPEKFSAFLDKVVGLQK in isoform 2.		VSP_039304
	Alternative sequence	166 – 1166	1001	Missing in isoform 2.		VSP_039305

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 5F045ACF188BBE8A	FASTA	1,166	128,664
	10 20 30 40 50 60 MKRAFVTVGT TSFDELVARV VANDCVQILE SLGYNHLVLQ VGRGTVVPKP FRTESFTLDV 70 80 90 100 110 120 YRYKDSLKED LQQADLVISH AGAGSCLESL EKGKPLVVVV NEKLMNNHQF ELAKQLHKEG 130 140 150 160 170 180 HLFYCTCRVL SCPAPVSLLL VLLGSAKILQ QLPSATLSCF GYLPTQAPVL VATAYSYLHS 190 200 210 220 230 240 VFSSFPPLST FLIIPCTMQK GWKKYCGQKS LNEASMDEYL GSLGLFRKVV AKDASCLFRA 250 260 270 280 290 300 ISEQLFHSQI HHLQIRRACV SYMKENQQAF ESYVEGSFEK YLERLGDPKE SAGQLELKAL 310 320 330 340 350 360 SLIYNRDFII YRYPGKPPTQ VTDNGFEDKI ILCYSNNGHY DSVYSKEFQS TAGICQAILY 370 380 390 400 410 420 ELLYKDVFVV DEETLKTAVD LFRSGSRRNK HHALTASVEG SSDQKSSTED RTEEAAACSS 430 440 450 460 470 480 AASTPEGNKQ GTERQKVPES PSKMLFPYKV LKALDPEIYR NVEFDAWLDS RKELQKSECV 490 500 510 520 530 540 EYGGRYYFLG DKCQVCMESG GKYYNAHIQE IDNDKSSVVV FIEEFAERHS IPLAHVRPVN 550 560 570 580 590 600 QVALLPSWNA IPIRNGRGYP TITGGYFPEI VMTDMNMKQR KKMFKKFRGK EIYMTMAYSR 610 620 630 640 650 660 GDPLVPSRIQ HSMHYGHDPL LYYSQTAGHI LSSQHFYPQH SSQRQGRGYG MPRDSSHLIS 670 680 690 700 710 720 KQNLPNPKVG FCSGSGRKCC QSYDNVSYRS RSFRRSHRQM HCMNKGCQYG FAPENGVEET 730 740 750 760 770 780 VTFYALEEGN ETAYSTLPNN GGPTTMVPAT SGYCVARQGY NSCKPPLNSG DSNDHCDNGG 790 800 810 820 830 840 YHGDYLYSSE QGYETSSVYT TTVSTANLSL QDSGPSSVPQ DTVTSYNYPQ KVLENSAAIA 850 860 870 880 890 900 VSWASHVPVP VIPNCAGDNE ALRTSDISSQ NAIQPGFVPP PAQGSPAYLE PSAAGAAGAA 910 920 930 940 950 960 AAAAAAAAPV ATPVAAPLPL PPPLPPPPPA TLEAGDASGF PLPPPPPPPP PPPPPYSYDP 970 980 990 1000 1010 1020 SGSDLPQDTK VLQYYFNLGL QCYHHNYWHP MVYMPHVQQQ LQPQPQPQPQ PQPQPQPQPQ 1030 1040 1050 1060 1070 1080 PQPQQPQQQQ PPPQQQQQQQ EQVHGESYPD CTEQPLVDQS APQVYSDVVR EDGTQADVST 1090 1100 1110 1120 1130 1140 NDTFPIADAV PLPHGAVYYP VMTDPYGSPL LGFDSYVPVA SDYSSIAMWH PVNAACGASA 1150 1160 QIHGAMNPGP IGYMLLPNSP HYTPQN
	Isoform 2 [UniParc]. Checksum: 947DFE404D54FA8C	FASTA	165	18,346
	10 20 30 40 50 60 MKRAFVTVGT TSFDELVARV VANDCVQILE SLGYNHLVLQ VGRGTVVPKP FRTESFTLDV 70 80 90 100 110 120 YRYKDSLKED LQQADLVISH AGAGSCLESL EKGKPLVVVV NEKLMNNHQF ELAKQLHKEG 130 140 150 160 HLFYCTCSTL PGLLQSMDLS TLKCYPPGQP EKFSAFLDKV VGLQK

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J and NOD. Tissue: Head, Small intestine and Thymus.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Fetal brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK008162 mRNA. Translation: BAB25504.1. AK014790 mRNA. Translation: BAB29554.1. AK086546 mRNA. Translation: BAC39689.1. AK089102 mRNA. Translation: BAC40750.1. AL713978 Genomic DNA. Translation: CAM45936.1. AL713978 Genomic DNA. Translation: CAM45938.1. Sequence problems. AL713978 Genomic DNA. Translation: CAM45939.1. Sequence problems. CH466610 Genomic DNA. Translation: EDL14725.1. BC053004 mRNA. No translation available. BC137692 mRNA. Translation: AAI37693.1.
IPI	IPI00108727. IPI00968337.
RefSeq	NP_080523.2. NM_026247.3.
UniGene	Mm.249084. Mm.484110.
3D structure databases
ProteinModelPortal	Q9D8C3.
SMR	Q9D8C3. Positions 2-127, 213-344, 486-541.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9D8C3.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PRIDE	Q9D8C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000070801; ENSMUSP00000068403; ENSMUSG00000041718.
GeneID	67574.
KEGG	mmu:67574.
UCSC	uc008pqx.2. mouse.
Organism-specific databases
CTD	79868.
MGI	MGI:1914824. Alg13.
Phylogenomic databases
GeneTree	ENSGT00530000063508.
HOGENOM	HBG629711.
HOVERGEN	HBG056051.
InParanoid	Q9D8C3.
OMA	KRAFVTV.
Gene expression databases
ArrayExpress	Q9D8C3.
Bgee	Q9D8C3.
Genevestigator	Q9D8C3.
GermOnline	ENSMUSG00000041718. Mus musculus.
Family and domain databases
InterPro	IPR007235. Glyco_trans_28_C. IPR003323. OTU. IPR018351. Tudor_subgr. [Graphical view]
KO	K07432.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS50304. TUDOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	324945.
SOURCE	Search...

Entry information

Entry name

ALG13_MOUSE

Accession

Primary (citable) accession number: Q9D8C3
Secondary accession number(s): A3KGC8 , A3KGD0, A3KGD1, Q8BUA4, Q9D5Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 15, 2010
Last modified:	December 14, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program