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Q9D8C3 (ALG13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13

EC=2.4.1.141
EC=3.4.19.12
Alternative name(s):
Asparagine-linked glycosylation 13 homolog
Glycosyltransferase 28 domain-containing protein 1
UDP-N-acetylglucosamine transferase subunit ALG13 homolog
Gene names
Name:Alg13
Synonyms:Glt28d1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1166 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Isoform 1:Possible multifunctional enzyme with both glycosyltransferase and deubiquitinase activities.

Isoform 2:May be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway (By. similarity).

Catalytic activity

UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Isoform 2 may interact with ALG14 By similarity.

Subcellular location

Endoplasmic reticulum By similarity. Note: Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14 By similarity.

Sequence similarities

Belongs to the glycosyltransferase 28 family.

Contains 1 OTU domain.

Contains 1 Tudor domain.

Sequence caution

The sequence CAM45938.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAM45939.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9D8C3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9D8C3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     128-165: RVLSCPAPVS...TLSCFGYLPT → STLPGLLQSM...FLDKVVGLQK
     166-1166: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11661166Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13
PRO_0000254574

Regions

Domain225 – 346122OTU
Domain486 – 54661Tudor
Region1 – 125125Glycosyltransferase activity By similarity
Region126 – 394269Deubiquitinase activity By similarity
Compositional bias875 – 1033159Pro-rich

Sites

Active site2331For deubiquitinase activity By similarity
Active site2361Nucleophile; for deubiquitinase activity By similarity
Active site3391For deubiquitinase activity By similarity

Natural variations

Alternative sequence128 – 16538RVLSC…GYLPT → STLPGLLQSMDLSTLKCYPP GQPEKFSAFLDKVVGLQK in isoform 2.
VSP_039304
Alternative sequence166 – 11661001Missing in isoform 2.
VSP_039305

Experimental info

Isoform 2:
Sequence conflict1491Q → H in BAB29554. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: 5F045ACF188BBE8A

FASTA1,166128,664
        10         20         30         40         50         60 
MKRAFVTVGT TSFDELVARV VANDCVQILE SLGYNHLVLQ VGRGTVVPKP FRTESFTLDV 

        70         80         90        100        110        120 
YRYKDSLKED LQQADLVISH AGAGSCLESL EKGKPLVVVV NEKLMNNHQF ELAKQLHKEG 

       130        140        150        160        170        180 
HLFYCTCRVL SCPAPVSLLL VLLGSAKILQ QLPSATLSCF GYLPTQAPVL VATAYSYLHS 

       190        200        210        220        230        240 
VFSSFPPLST FLIIPCTMQK GWKKYCGQKS LNEASMDEYL GSLGLFRKVV AKDASCLFRA 

       250        260        270        280        290        300 
ISEQLFHSQI HHLQIRRACV SYMKENQQAF ESYVEGSFEK YLERLGDPKE SAGQLELKAL 

       310        320        330        340        350        360 
SLIYNRDFII YRYPGKPPTQ VTDNGFEDKI ILCYSNNGHY DSVYSKEFQS TAGICQAILY 

       370        380        390        400        410        420 
ELLYKDVFVV DEETLKTAVD LFRSGSRRNK HHALTASVEG SSDQKSSTED RTEEAAACSS 

       430        440        450        460        470        480 
AASTPEGNKQ GTERQKVPES PSKMLFPYKV LKALDPEIYR NVEFDAWLDS RKELQKSECV 

       490        500        510        520        530        540 
EYGGRYYFLG DKCQVCMESG GKYYNAHIQE IDNDKSSVVV FIEEFAERHS IPLAHVRPVN 

       550        560        570        580        590        600 
QVALLPSWNA IPIRNGRGYP TITGGYFPEI VMTDMNMKQR KKMFKKFRGK EIYMTMAYSR 

       610        620        630        640        650        660 
GDPLVPSRIQ HSMHYGHDPL LYYSQTAGHI LSSQHFYPQH SSQRQGRGYG MPRDSSHLIS 

       670        680        690        700        710        720 
KQNLPNPKVG FCSGSGRKCC QSYDNVSYRS RSFRRSHRQM HCMNKGCQYG FAPENGVEET 

       730        740        750        760        770        780 
VTFYALEEGN ETAYSTLPNN GGPTTMVPAT SGYCVARQGY NSCKPPLNSG DSNDHCDNGG 

       790        800        810        820        830        840 
YHGDYLYSSE QGYETSSVYT TTVSTANLSL QDSGPSSVPQ DTVTSYNYPQ KVLENSAAIA 

       850        860        870        880        890        900 
VSWASHVPVP VIPNCAGDNE ALRTSDISSQ NAIQPGFVPP PAQGSPAYLE PSAAGAAGAA 

       910        920        930        940        950        960 
AAAAAAAAPV ATPVAAPLPL PPPLPPPPPA TLEAGDASGF PLPPPPPPPP PPPPPYSYDP 

       970        980        990       1000       1010       1020 
SGSDLPQDTK VLQYYFNLGL QCYHHNYWHP MVYMPHVQQQ LQPQPQPQPQ PQPQPQPQPQ 

      1030       1040       1050       1060       1070       1080 
PQPQQPQQQQ PPPQQQQQQQ EQVHGESYPD CTEQPLVDQS APQVYSDVVR EDGTQADVST 

      1090       1100       1110       1120       1130       1140 
NDTFPIADAV PLPHGAVYYP VMTDPYGSPL LGFDSYVPVA SDYSSIAMWH PVNAACGASA 

      1150       1160 
QIHGAMNPGP IGYMLLPNSP HYTPQN 

« Hide

Isoform 2 [UniParc].

Checksum: 947DFE404D54FA8C
Show »

FASTA16518,346

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J and NOD.
Tissue: Head, Small intestine and Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Fetal brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK008162 mRNA. Translation: BAB25504.1.
AK014790 mRNA. Translation: BAB29554.1.
AK086546 mRNA. Translation: BAC39689.1.
AK089102 mRNA. Translation: BAC40750.1.
AL713978 Genomic DNA. Translation: CAM45936.1.
AL713978 Genomic DNA. Translation: CAM45938.1. Sequence problems.
AL713978 Genomic DNA. Translation: CAM45939.1. Sequence problems.
CH466610 Genomic DNA. Translation: EDL14725.1.
BC053004 mRNA. No translation available.
BC137692 mRNA. Translation: AAI37693.1.
RefSeqNP_080523.2. NM_026247.3.
UniGeneMm.249084.
Mm.484110.

3D structure databases

ProteinModelPortalQ9D8C3.
SMRQ9D8C3. Positions 2-176, 215-343, 485-541.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000042810.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteQ9D8C3.

Proteomic databases

PaxDbQ9D8C3.
PRIDEQ9D8C3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070801; ENSMUSP00000068403; ENSMUSG00000041718. [Q9D8C3-2]
GeneID67574.
KEGGmmu:67574.
UCSCuc009ums.2. mouse. [Q9D8C3-2]
uc009umu.2. mouse. [Q9D8C3-1]

Organism-specific databases

CTD79868.
MGIMGI:1914824. Alg13.

Phylogenomic databases

eggNOGCOG5017.
GeneTreeENSGT00510000047493.
HOGENOMHOG000192807.
HOVERGENHBG056051.
InParanoidA3KGC8.
KOK07432.
OMALVIQMGR.
OrthoDBEOG7NPFVV.
PhylomeDBQ9D8C3.
TreeFamTF313788.

Gene expression databases

BgeeQ9D8C3.
GenevestigatorQ9D8C3.

Family and domain databases

InterProIPR007235. Glyco_trans_28_C.
IPR003323. OTU.
IPR002999. Tudor.
[Graphical view]
PfamPF04101. Glyco_tran_28_C. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio324945.
PROQ9D8C3.
SOURCESearch...

Entry information

Entry nameALG13_MOUSE
AccessionPrimary (citable) accession number: Q9D8C3
Secondary accession number(s): A3KGC8 expand/collapse secondary AC list , A3KGD0, A3KGD1, Q8BUA4, Q9D5Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 15, 2010
Last modified: April 16, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot