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Protein

Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13

Gene

Alg13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: Possible multifunctional enzyme with both glycosyltransferase and deubiquitinase activities.
Isoform 2: May be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway (By. similarity).

Catalytic activityi

UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei233For deubiquitinase activityBy similarity1
Active sitei236Nucleophile; for deubiquitinase activityBy similarity1
Active sitei339For deubiquitinase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Protease, Thiol protease, Transferase

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 (EC:2.4.1.141, EC:3.4.19.12)
Alternative name(s):
Asparagine-linked glycosylation 13 homolog
Glycosyltransferase 28 domain-containing protein 1
UDP-N-acetylglucosamine transferase subunit ALG13 homolog
Gene namesi
Name:Alg13
Synonyms:Glt28d1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1914824. Alg13.

Subcellular locationi

  • Endoplasmic reticulum By similarity

  • Note: Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002545741 – 1166Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13Add BLAST1166

Proteomic databases

PeptideAtlasiQ9D8C3.
PRIDEiQ9D8C3.

PTM databases

PhosphoSitePlusiQ9D8C3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041718.
ExpressionAtlasiQ9D8C3. baseline and differential.
GenevisibleiQ9D8C3. MM.

Interactioni

Subunit structurei

Isoform 2 may interact with ALG14.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9D8C3.
SMRiQ9D8C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini225 – 346OTUPROSITE-ProRule annotationAdd BLAST122
Domaini486 – 546TudorPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 125Glycosyltransferase activityBy similarityAdd BLAST125
Regioni126 – 394Deubiquitinase activityBy similarityAdd BLAST269

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi875 – 1033Pro-richAdd BLAST159

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 1 Tudor domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00510000047493.
HOGENOMiHOG000192807.
HOVERGENiHBG056051.
InParanoidiQ9D8C3.
KOiK07432.
OMAiWHASLVI.
OrthoDBiEOG091G0SQ3.
PhylomeDBiQ9D8C3.
TreeFamiTF313788.

Family and domain databases

InterProiIPR007235. Glyco_trans_28_C.
IPR003323. OTU_dom.
IPR002999. Tudor.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D8C3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRAFVTVGT TSFDELVARV VANDCVQILE SLGYNHLVLQ VGRGTVVPKP
60 70 80 90 100
FRTESFTLDV YRYKDSLKED LQQADLVISH AGAGSCLESL EKGKPLVVVV
110 120 130 140 150
NEKLMNNHQF ELAKQLHKEG HLFYCTCRVL SCPAPVSLLL VLLGSAKILQ
160 170 180 190 200
QLPSATLSCF GYLPTQAPVL VATAYSYLHS VFSSFPPLST FLIIPCTMQK
210 220 230 240 250
GWKKYCGQKS LNEASMDEYL GSLGLFRKVV AKDASCLFRA ISEQLFHSQI
260 270 280 290 300
HHLQIRRACV SYMKENQQAF ESYVEGSFEK YLERLGDPKE SAGQLELKAL
310 320 330 340 350
SLIYNRDFII YRYPGKPPTQ VTDNGFEDKI ILCYSNNGHY DSVYSKEFQS
360 370 380 390 400
TAGICQAILY ELLYKDVFVV DEETLKTAVD LFRSGSRRNK HHALTASVEG
410 420 430 440 450
SSDQKSSTED RTEEAAACSS AASTPEGNKQ GTERQKVPES PSKMLFPYKV
460 470 480 490 500
LKALDPEIYR NVEFDAWLDS RKELQKSECV EYGGRYYFLG DKCQVCMESG
510 520 530 540 550
GKYYNAHIQE IDNDKSSVVV FIEEFAERHS IPLAHVRPVN QVALLPSWNA
560 570 580 590 600
IPIRNGRGYP TITGGYFPEI VMTDMNMKQR KKMFKKFRGK EIYMTMAYSR
610 620 630 640 650
GDPLVPSRIQ HSMHYGHDPL LYYSQTAGHI LSSQHFYPQH SSQRQGRGYG
660 670 680 690 700
MPRDSSHLIS KQNLPNPKVG FCSGSGRKCC QSYDNVSYRS RSFRRSHRQM
710 720 730 740 750
HCMNKGCQYG FAPENGVEET VTFYALEEGN ETAYSTLPNN GGPTTMVPAT
760 770 780 790 800
SGYCVARQGY NSCKPPLNSG DSNDHCDNGG YHGDYLYSSE QGYETSSVYT
810 820 830 840 850
TTVSTANLSL QDSGPSSVPQ DTVTSYNYPQ KVLENSAAIA VSWASHVPVP
860 870 880 890 900
VIPNCAGDNE ALRTSDISSQ NAIQPGFVPP PAQGSPAYLE PSAAGAAGAA
910 920 930 940 950
AAAAAAAAPV ATPVAAPLPL PPPLPPPPPA TLEAGDASGF PLPPPPPPPP
960 970 980 990 1000
PPPPPYSYDP SGSDLPQDTK VLQYYFNLGL QCYHHNYWHP MVYMPHVQQQ
1010 1020 1030 1040 1050
LQPQPQPQPQ PQPQPQPQPQ PQPQQPQQQQ PPPQQQQQQQ EQVHGESYPD
1060 1070 1080 1090 1100
CTEQPLVDQS APQVYSDVVR EDGTQADVST NDTFPIADAV PLPHGAVYYP
1110 1120 1130 1140 1150
VMTDPYGSPL LGFDSYVPVA SDYSSIAMWH PVNAACGASA QIHGAMNPGP
1160
IGYMLLPNSP HYTPQN
Length:1,166
Mass (Da):128,664
Last modified:June 15, 2010 - v2
Checksum:i5F045ACF188BBE8A
GO
Isoform 2 (identifier: Q9D8C3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-165: RVLSCPAPVS...TLSCFGYLPT → STLPGLLQSM...FLDKVVGLQK
     166-1166: Missing.

Show »
Length:165
Mass (Da):18,346
Checksum:i947DFE404D54FA8C
GO

Sequence cautioni

The sequence CAM45938 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM45939 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q9D8C3-2)
Sequence conflicti149Q → H in BAB29554 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039304128 – 165RVLSC…GYLPT → STLPGLLQSMDLSTLKCYPP GQPEKFSAFLDKVVGLQK in isoform 2. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_039305166 – 1166Missing in isoform 2. 2 PublicationsAdd BLAST1001

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008162 mRNA. Translation: BAB25504.1.
AK014790 mRNA. Translation: BAB29554.1.
AK086546 mRNA. Translation: BAC39689.1.
AK089102 mRNA. Translation: BAC40750.1.
AL713978 Genomic DNA. Translation: CAM45936.1.
AL713978 Genomic DNA. Translation: CAM45938.1. Sequence problems.
AL713978 Genomic DNA. Translation: CAM45939.1. Sequence problems.
CH466610 Genomic DNA. Translation: EDL14725.1.
BC053004 mRNA. No translation available.
BC137692 mRNA. Translation: AAI37693.1.
CCDSiCCDS30456.1. [Q9D8C3-2]
RefSeqiNP_080523.2. NM_026247.3. [Q9D8C3-2]
UniGeneiMm.249084.
Mm.484110.

Genome annotation databases

EnsembliENSMUST00000070801; ENSMUSP00000068403; ENSMUSG00000041718. [Q9D8C3-2]
GeneIDi67574.
KEGGimmu:67574.
UCSCiuc009ums.2. mouse. [Q9D8C3-2]
uc009umu.2. mouse. [Q9D8C3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008162 mRNA. Translation: BAB25504.1.
AK014790 mRNA. Translation: BAB29554.1.
AK086546 mRNA. Translation: BAC39689.1.
AK089102 mRNA. Translation: BAC40750.1.
AL713978 Genomic DNA. Translation: CAM45936.1.
AL713978 Genomic DNA. Translation: CAM45938.1. Sequence problems.
AL713978 Genomic DNA. Translation: CAM45939.1. Sequence problems.
CH466610 Genomic DNA. Translation: EDL14725.1.
BC053004 mRNA. No translation available.
BC137692 mRNA. Translation: AAI37693.1.
CCDSiCCDS30456.1. [Q9D8C3-2]
RefSeqiNP_080523.2. NM_026247.3. [Q9D8C3-2]
UniGeneiMm.249084.
Mm.484110.

3D structure databases

ProteinModelPortaliQ9D8C3.
SMRiQ9D8C3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSitePlusiQ9D8C3.

Proteomic databases

PeptideAtlasiQ9D8C3.
PRIDEiQ9D8C3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070801; ENSMUSP00000068403; ENSMUSG00000041718. [Q9D8C3-2]
GeneIDi67574.
KEGGimmu:67574.
UCSCiuc009ums.2. mouse. [Q9D8C3-2]
uc009umu.2. mouse. [Q9D8C3-1]

Organism-specific databases

CTDi79868.
MGIiMGI:1914824. Alg13.

Phylogenomic databases

GeneTreeiENSGT00510000047493.
HOGENOMiHOG000192807.
HOVERGENiHBG056051.
InParanoidiQ9D8C3.
KOiK07432.
OMAiWHASLVI.
OrthoDBiEOG091G0SQ3.
PhylomeDBiQ9D8C3.
TreeFamiTF313788.

Miscellaneous databases

PROiQ9D8C3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041718.
ExpressionAtlasiQ9D8C3. baseline and differential.
GenevisibleiQ9D8C3. MM.

Family and domain databases

InterProiIPR007235. Glyco_trans_28_C.
IPR003323. OTU_dom.
IPR002999. Tudor.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG13_MOUSE
AccessioniPrimary (citable) accession number: Q9D8C3
Secondary accession number(s): A3KGC8
, A3KGD0, A3KGD1, Q8BUA4, Q9D5Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 15, 2010
Last modified: November 30, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.