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Protein

Junctional adhesion molecule C

Gene

Jam3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in cell-cell adhesion. It is a counter-receptor for ITGAM mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (By similarity). The soluble form is a mediator of angiogenesis.By similarity3 Publications

GO - Molecular functioni

  • integrin binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • adaptive immune response Source: MGI
  • angiogenesis Source: UniProtKB
  • axon regeneration Source: Ensembl
  • cell adhesion Source: MGI
  • cell-matrix adhesion Source: MGI
  • cell migration Source: MGI
  • establishment of cell polarity Source: MGI
  • leukocyte migration involved in inflammatory response Source: MGI
  • myelination Source: MGI
  • myeloid progenitor cell differentiation Source: MGI
  • neutrophil homeostasis Source: MGI
  • regulation of actin cytoskeleton organization by cell-cell adhesion Source: MGI
  • regulation of neutrophil chemotaxis Source: UniProtKB
  • spermatid development Source: MGI
  • spermatogenesis Source: MGI
  • transmission of nerve impulse Source: MGI
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule C
Short name:
JAM-C
Alternative name(s):
JAM-2
Junctional adhesion molecule 3
Short name:
JAM-3
Gene namesi
Name:Jam3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1933825. Jam3.

Subcellular locationi

  • Cell membrane Curated; Single-pass type I membrane protein Curated
  • Cell junctiondesmosome By similarity
  • Secretedextracellular space By similarity

  • Note: In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells. Treatment of endothelial cells with vascular endothelial growth factor stimulates recruitment of JAM3 to cell-cell contacts.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 241212ExtracellularSequence analysisAdd
BLAST
Transmembranei242 – 26221HelicalSequence analysisAdd
BLAST
Topological domaini263 – 31048CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • bicellular tight junction Source: MGI
  • cell-cell contact zone Source: MGI
  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • desmosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • paranodal junction Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • Schmidt-Lanterman incisure Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 310281Junctional adhesion molecule CPRO_0000015072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 115PROSITE-ProRule annotation
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence analysis
Disulfide bondi160 ↔ 219PROSITE-ProRule annotation
Glycosylationi192 – 1921N-linked (GlcNAc...)1 Publication
Glycosylationi198 – 1981N-linked (GlcNAc...); atypical1 Publication

Post-translational modificationi

Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9D8B7.
PaxDbiQ9D8B7.
PRIDEiQ9D8B7.

PTM databases

iPTMnetiQ9D8B7.
PhosphoSiteiQ9D8B7.
SwissPalmiQ9D8B7.

Expressioni

Tissue specificityi

Endothelial cells.1 Publication

Gene expression databases

BgeeiQ9D8B7.
CleanExiMM_JAM3.
ExpressionAtlasiQ9D8B7. baseline and differential.
GenevisibleiQ9D8B7. MM.

Interactioni

Subunit structurei

Interacts with JAM2. Interacts with ITGAM.By similarity

GO - Molecular functioni

  • integrin binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

DIPiDIP-42154N.
MINTiMINT-1344650.
STRINGi10090.ENSMUSP00000034472.

Structurei

3D structure databases

ProteinModelPortaliQ9D8B7.
SMRiQ9D8B7. Positions 43-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 12793Ig-like V-typeAdd
BLAST
Domaini139 – 23698Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGQI. Eukaryota.
ENOG41100KX. LUCA.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiQ9D8B7.
KOiK06785.
OMAiSYKNPGK.
PhylomeDBiQ9D8B7.
TreeFamiTF331459.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D8B7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSRRLRLR LYARLPDFFL LLLFRGCMIE AVNLKSSNRN PVVHEFESVE
60 70 80 90 100
LSCIITDSQT SDPRIEWKKI QDGQTTYVYF DNKIQGDLAG RTDVFGKTSL
110 120 130 140 150
RIWNVTRSDS AIYRCEVVAL NDRKEVDEIT IELIVQVKPV TPVCRIPAAV
160 170 180 190 200
PVGKTATLQC QESEGYPRPH YSWYRNDVPL PTDSRANPRF QNSSFHVNSE
210 220 230 240 250
TGTLVFNAVH KDDSGQYYCI ASNDAGAARC EGQDMEVYDL NIAGIIGGVL
260 270 280 290 300
VVLIVLAVIT MGICCAYRRG CFISSKQDGE SYKSPGKHDG VNYIRTSEEG
310
DFRHKSSFVI
Length:310
Mass (Da):34,838
Last modified:May 10, 2005 - v2
Checksum:i4B92BCB51D0A4B0A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441H → Q in BAB25519 (PubMed:16141072).Curated
Sequence conflicti172 – 1721S → N in BAB25519 (PubMed:16141072).Curated
Sequence conflicti303 – 3031R → K in BAB22715 (PubMed:16141072).Curated
Sequence conflicti306 – 3072SS → IA in BAB22715 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ300304 mRNA. Translation: CAC20704.1.
AK008187 mRNA. Translation: BAB25519.1.
AK003326 mRNA. Translation: BAB22715.1.
AK013156 mRNA. Translation: BAB28683.1.
AK017692 mRNA. Translation: BAC25526.1.
AK032833 mRNA. Translation: BAC28049.1.
BC024357 mRNA. Translation: AAH24357.1.
CCDSiCCDS22939.1.
RefSeqiNP_075766.1. NM_023277.4.
UniGeneiMm.28770.

Genome annotation databases

EnsembliENSMUST00000034472; ENSMUSP00000034472; ENSMUSG00000031990.
GeneIDi83964.
KEGGimmu:83964.
UCSCiuc009oqj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ300304 mRNA. Translation: CAC20704.1.
AK008187 mRNA. Translation: BAB25519.1.
AK003326 mRNA. Translation: BAB22715.1.
AK013156 mRNA. Translation: BAB28683.1.
AK017692 mRNA. Translation: BAC25526.1.
AK032833 mRNA. Translation: BAC28049.1.
BC024357 mRNA. Translation: AAH24357.1.
CCDSiCCDS22939.1.
RefSeqiNP_075766.1. NM_023277.4.
UniGeneiMm.28770.

3D structure databases

ProteinModelPortaliQ9D8B7.
SMRiQ9D8B7. Positions 43-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-42154N.
MINTiMINT-1344650.
STRINGi10090.ENSMUSP00000034472.

PTM databases

iPTMnetiQ9D8B7.
PhosphoSiteiQ9D8B7.
SwissPalmiQ9D8B7.

Proteomic databases

MaxQBiQ9D8B7.
PaxDbiQ9D8B7.
PRIDEiQ9D8B7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034472; ENSMUSP00000034472; ENSMUSG00000031990.
GeneIDi83964.
KEGGimmu:83964.
UCSCiuc009oqj.2. mouse.

Organism-specific databases

CTDi83700.
MGIiMGI:1933825. Jam3.

Phylogenomic databases

eggNOGiENOG410IGQI. Eukaryota.
ENOG41100KX. LUCA.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiQ9D8B7.
KOiK06785.
OMAiSYKNPGK.
PhylomeDBiQ9D8B7.
TreeFamiTF331459.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.

Miscellaneous databases

ChiTaRSiJam3. mouse.
NextBioi350838.
PROiQ9D8B7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D8B7.
CleanExiMM_JAM3.
ExpressionAtlasiQ9D8B7. baseline and differential.
GenevisibleiQ9D8B7. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of JAM-2 and JAM-3: an emerging junctional adhesion molecular family?"
    Aurrand-Lions M.A., Duncan L., Du Pasquier L., Imhof B.A.
    Curr. Top. Microbiol. Immunol. 251:91-98(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells."
    Aurrand-Lions M.A., Duncan L., Ballestrem C., Imhof B.A.
    J. Biol. Chem. 276:2733-2741(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Small intestine and Wolffian duct.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  5. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 84-91 AND 146-154, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  6. "Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members."
    Aurrand-Lions M.A., Johnson-Leger C., Wong C., Du Pasquier L., Imhof B.A.
    Blood 98:3699-3707(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  7. "Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response."
    Muller W.A.
    Trends Immunol. 24:327-334(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, NOMENCLATURE.
  8. "Antibody against junctional adhesion molecule-C inhibits angiogenesis and tumor growth."
    Lamagna C., Hodivala-Dilke K.M., Imhof B.A., Aurrand-Lions M.
    Cancer Res. 65:5703-5710(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ANGIOGENESIS.
  9. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-192 AND ASN-198.
    Tissue: Myoblast.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Testis.
  11. Cited for: FUNCTION IN ANGIOGENESIS.

Entry informationi

Entry nameiJAM3_MOUSE
AccessioniPrimary (citable) accession number: Q9D8B7
Secondary accession number(s): Q8BT59, Q9D1M9, Q9EPK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: May 11, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.