Q9D8B7 (JAM3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Junctional adhesion molecule C
Short name=JAM-C

Alternative name(s):
JAM-2
Junctional adhesion molecule 3
Short name=JAM-3

Gene names

Name:

Jam3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in cell-cell adhesion. It is a counterreceptor for ITGAM mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) By similarity. The soluble form is a mediator of angiogenesis. Ref.6 Ref.8 Ref.10
Subunit structure	Interacts with JAM2 By similarity. Interacts with ITGAM By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein Potential. Cell junction › desmosome By similarity. Secreted › extracellular space By similarity. Note: In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells. Treatment of endothelial cells with vascular endothelial growth factor stimulates recruitement of JAM3 to cell-cell contacts By similarity.
Tissue specificity	Endothelial cells. Ref.6
Post-translational modification	Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors By similarity.
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Angiogenesis Cell adhesion
Cellular component	Cell junction Cell membrane Membrane Secreted
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	adaptive immune response Inferred from mutant phenotype. Source: MGI angiogenesis Inferred from direct assay Ref.8. Source: UniProtKB cell-matrix adhesion Inferred from direct assay. Source: MGI establishment of cell polarity Inferred from direct assay. Source: MGI leukocyte migration involved in inflammatory response Inferred from mutant phenotype. Source: MGI myelination Inferred from mutant phenotype. Source: MGI myeloid progenitor cell differentiation Inferred from mutant phenotype. Source: MGI neutrophil homeostasis Inferred from mutant phenotype. Source: MGI regulation of actin cytoskeleton organization by cell-cell adhesion Inferred from mutant phenotype. Source: MGI regulation of neutrophil chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB spermatid development Inferred from mutant phenotype. Source: MGI
Cellular component	desmosome Inferred from sequence or structural similarity. Source: UniProtKB extracellular space Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW paranodal junction Inferred from direct assay. Source: MGI tight junction Inferred from direct assay. Source: MGI
Molecular function	integrin binding Inferred from physical interaction. Source: MGI protein heterodimerization activity Inferred from physical interaction. Source: MGI protein homodimerization activity Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 310	281	Junctional adhesion molecule C		PRO_0000015072
Regions
	Topological domain	30 – 241	212	Extracellular Potential
	Transmembrane	242 – 262	21	Helical; Potential
	Topological domain	263 – 310	48	Cytoplasmic Potential
	Domain	35 – 127	93	Ig-like V-type
	Domain	139 – 236	98	Ig-like C2-type
Amino acid modifications
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	198	1	N-linked (GlcNAc...) Ref.9
	Disulfide bond	53 ↔ 115		Potential
	Disulfide bond	160 ↔ 219		Potential
Experimental info
	Sequence conflict	44	1	H → Q in BAB25519. Ref.3
	Sequence conflict	172	1	S → N in BAB25519. Ref.3
	Sequence conflict	303	1	R → K in BAB22715. Ref.3
	Sequence conflict	306 – 307	2	SS → IA in BAB22715. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9D8B7 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 4B92BCB51D0A4B0A	FASTA	310	34,838
	10 20 30 40 50 60 MALSRRLRLR LYARLPDFFL LLLFRGCMIE AVNLKSSNRN PVVHEFESVE LSCIITDSQT 70 80 90 100 110 120 SDPRIEWKKI QDGQTTYVYF DNKIQGDLAG RTDVFGKTSL RIWNVTRSDS AIYRCEVVAL 130 140 150 160 170 180 NDRKEVDEIT IELIVQVKPV TPVCRIPAAV PVGKTATLQC QESEGYPRPH YSWYRNDVPL 190 200 210 220 230 240 PTDSRANPRF QNSSFHVNSE TGTLVFNAVH KDDSGQYYCI ASNDAGAARC EGQDMEVYDL 250 260 270 280 290 300 NIAGIIGGVL VVLIVLAVIT MGICCAYRRG CFISSKQDGE SYKSPGKHDG VNYIRTSEEG 310 DFRHKSSFVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of JAM-2 and JAM-3: an emerging junctional adhesion molecular family?" Aurrand-Lions M.A., Duncan L., Du Pasquier L., Imhof B.A. Curr. Top. Microbiol. Immunol. 251:91-98(2000) [PubMed: 11036763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells." Aurrand-Lions M.A., Duncan L., Ballestrem C., Imhof B.A. J. Biol. Chem. 276:2733-2741(2001) [PubMed: 11053409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo, Small intestine and Wolffian duct.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[5]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 84-91 AND 146-154, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[6]	"Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members." Aurrand-Lions M.A., Johnson-Leger C., Wong C., Du Pasquier L., Imhof B.A. Blood 98:3699-3707(2001) [PubMed: 11739175] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response." Muller W.A. Trends Immunol. 24:327-334(2003) [PubMed: 12810109] [Abstract] Cited for: REVIEW, NOMENCLATURE.
[8]	"Antibody against junctional adhesion molecule-C inhibits angiogenesis and tumor growth." Lamagna C., Hodivala-Dilke K.M., Imhof B.A., Aurrand-Lions M. Cancer Res. 65:5703-5710(2005) [PubMed: 15994945] [Abstract] Cited for: FUNCTION IN ANGIOGENESIS.
[9]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed: 19656770] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-192 AND ASN-198, MASS SPECTROMETRY. Tissue: Myoblast.
[10]	"Junctional adhesion molecule-C is a soluble mediator of angiogenesis." Rabquer B.J., Amin M.A., Teegala N., Shaheen M.K., Tsou P.S., Ruth J.H., Lesch C.A., Imhof B.A., Koch A.E. J. Immunol. 185:1777-1785(2010) [PubMed: 20592283] [Abstract] Cited for: FUNCTION IN ANGIOGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ300304 mRNA. Translation: CAC20704.1. AK008187 mRNA. Translation: BAB25519.1. AK003326 mRNA. Translation: BAB22715.1. AK013156 mRNA. Translation: BAB28683.1. AK017692 mRNA. Translation: BAC25526.1. AK032833 mRNA. Translation: BAC28049.1. BC024357 mRNA. Translation: AAH24357.1.
IPI	IPI00453847.
RefSeq	NP_075766.1. NM_023277.4.
UniGene	Mm.28770.
3D structure databases
ProteinModelPortal	Q9D8B7.
SMR	Q9D8B7. Positions 29-238.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1344650.
STRING	Q9D8B7.
Proteomic databases
PRIDE	Q9D8B7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034472; ENSMUSP00000034472; ENSMUSG00000031990.
GeneID	83964.
KEGG	mmu:83964.
UCSC	uc009oqj.1. mouse.
Organism-specific databases
CTD	83700.
MGI	MGI:1933825. Jam3.
Phylogenomic databases
eggNOG	roNOG09320.
GeneTree	ENSGT00600000084033.
HOGENOM	HBG446408.
HOVERGEN	HBG000518.
InParanoid	Q9D8B7.
OMA	YSWYRND.
OrthoDB	EOG4D26QH.
PhylomeDB	Q9D8B7.
Gene expression databases
ArrayExpress	Q9D8B7.
Bgee	Q9D8B7.
CleanEx	MM_JAM3.
Genevestigator	Q9D8B7.
GermOnline	ENSMUSG00000031990. Mus musculus.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013106. Ig_V-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K06785.
Pfam	PF07679. I-set. 1 hit. PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	350838.
SOURCE	Search...

Entry information

Entry name

JAM3_MOUSE

Accession

Primary (citable) accession number: Q9D8B7
Secondary accession number(s): Q8BT59, Q9D1M9, Q9EPK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program