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Protein

Inosine triphosphate pyrophosphatase

Gene

Itpa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.UniRule annotation3 Publications

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.UniRule annotation

Kineticsi

Vmax values are similar for dITP, dHAPTP and dGTP.

  1. KM=24.9 µM for dITP1 Publication
  2. KM=38.4 µM for dHAPTP1 Publication
  3. KM=667 µM for dGTP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Magnesium or manganeseUniRule annotation
    Binding sitei56 – 561SubstrateUniRule annotation
    Metal bindingi72 – 721Magnesium or manganeseUniRule annotation
    Binding sitei172 – 1721SubstrateUniRule annotation

    GO - Molecular functioni

    • dITP diphosphatase activity Source: MGI
    • ITP diphosphatase activity Source: MGI
    • metal ion binding Source: UniProtKB-KW
    • nucleoside-triphosphate diphosphatase activity Source: MGI
    • nucleotide binding Source: UniProtKB-KW
    • XTP diphosphatase activity Source: MGI

    GO - Biological processi

    • chromosome organization Source: MGI
    • deoxyribonucleoside triphosphate catabolic process Source: UniProtKB-HAMAP
    • ITP catabolic process Source: MGI
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-MMU-15869. Metabolism of nucleotides.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine triphosphate pyrophosphataseUniRule annotation (EC:3.6.1.19UniRule annotation)
    Short name:
    ITPaseUniRule annotation
    Short name:
    Inosine triphosphataseUniRule annotation
    Alternative name(s):
    Non-canonical purine NTP pyrophosphataseUniRule annotation
    Non-standard purine NTP pyrophosphataseUniRule annotation
    Nucleoside-triphosphate diphosphataseUniRule annotation
    Nucleoside-triphosphate pyrophosphataseUniRule annotation
    Short name:
    NTPaseUniRule annotation
    Gene namesi
    Name:Itpa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 2

    Organism-specific databases

    MGIiMGI:96622. Itpa.

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Pups die about 2 weeks after birth with growth retardation and heart failure. Accumulates ITP in erythrocytes. Accumulates inosine in RNA and deoxyinosine in DNA.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemovedUniRule annotation
    Chaini2 – 198197Inosine triphosphate pyrophosphatasePRO_0000178281Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineUniRule annotationBy similarity
    Modified residuei146 – 1461PhosphoserineCombined sources

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiQ9D892.
    MaxQBiQ9D892.
    PaxDbiQ9D892.
    PRIDEiQ9D892.

    2D gel databases

    REPRODUCTION-2DPAGEQ9D892.

    PTM databases

    iPTMnetiQ9D892.
    PhosphoSiteiQ9D892.

    Expressioni

    Gene expression databases

    BgeeiQ9D892.
    ExpressionAtlasiQ9D892. baseline and differential.
    GenevisibleiQ9D892. MM.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    IntActiQ9D892. 4 interactions.
    MINTiMINT-1770686.
    STRINGi10090.ENSMUSP00000099482.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D892.
    SMRiQ9D892. Positions 1-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 196Substrate bindingUniRule annotation
    Regioni72 – 732Substrate bindingUniRule annotation
    Regioni149 – 1524Substrate bindingUniRule annotation
    Regioni177 – 1782Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the HAM1 NTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiKOG3222. Eukaryota.
    COG0127. LUCA.
    GeneTreeiENSGT00390000015399.
    HOGENOMiHOG000293320.
    HOVERGENiHBG039521.
    InParanoidiQ9D892.
    KOiK01519.
    OMAiPNFPRTI.
    OrthoDBiEOG7X0VJG.
    PhylomeDBiQ9D892.
    TreeFamiTF105614.

    Family and domain databases

    Gene3Di3.90.950.10. 1 hit.
    HAMAPiMF_03148. HAM1_NTPase.
    InterProiIPR002637. Ham1p-like.
    IPR027502. ITPase.
    IPR029001. ITPase-like_fam.
    [Graphical view]
    PANTHERiPTHR11067. PTHR11067. 1 hit.
    PfamiPF01725. Ham1p_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF52972. SSF52972. 1 hit.
    TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D892-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAASLVGKKI VFVTGNAKKL EEVIQILGDN FPCTLEAQKI DLPEYQGEPD
    60 70 80 90 100
    EISIQKCREA ARQVQGPVLV EDTCLCFNAL GGLPGPYIKW FLQKLKPEGL
    110 120 130 140 150
    HQLLAGFEDK SAYALCTFAL STGDPSQPVL LFRGQTSGQI VMPRGSRDFG
    160 170 180 190
    WDPCFQPDGY EQTYAEMPKS EKNTISHRFR ALHKLQEYFS VAAGAGDH
    Length:198
    Mass (Da):21,897
    Last modified:April 23, 2003 - v2
    Checksum:iD70337E3D2001A9E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti32 – 321P → Q in BAB25571 (PubMed:15489334).Curated
    Sequence conflicti54 – 541I → M in BAB25571 (PubMed:15489334).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK008279 mRNA. Translation: BAB25571.1.
    BC026508 mRNA. Translation: AAH26508.1.
    CCDSiCCDS16748.1.
    RefSeqiNP_080198.2. NM_025922.2.
    UniGeneiMm.21399.

    Genome annotation databases

    EnsembliENSMUST00000103193; ENSMUSP00000099482; ENSMUSG00000074797.
    GeneIDi16434.
    KEGGimmu:16434.
    UCSCiuc008mju.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK008279 mRNA. Translation: BAB25571.1.
    BC026508 mRNA. Translation: AAH26508.1.
    CCDSiCCDS16748.1.
    RefSeqiNP_080198.2. NM_025922.2.
    UniGeneiMm.21399.

    3D structure databases

    ProteinModelPortaliQ9D892.
    SMRiQ9D892. Positions 1-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ9D892. 4 interactions.
    MINTiMINT-1770686.
    STRINGi10090.ENSMUSP00000099482.

    PTM databases

    iPTMnetiQ9D892.
    PhosphoSiteiQ9D892.

    2D gel databases

    REPRODUCTION-2DPAGEQ9D892.

    Proteomic databases

    EPDiQ9D892.
    MaxQBiQ9D892.
    PaxDbiQ9D892.
    PRIDEiQ9D892.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000103193; ENSMUSP00000099482; ENSMUSG00000074797.
    GeneIDi16434.
    KEGGimmu:16434.
    UCSCiuc008mju.1. mouse.

    Organism-specific databases

    CTDi3704.
    MGIiMGI:96622. Itpa.

    Phylogenomic databases

    eggNOGiKOG3222. Eukaryota.
    COG0127. LUCA.
    GeneTreeiENSGT00390000015399.
    HOGENOMiHOG000293320.
    HOVERGENiHBG039521.
    InParanoidiQ9D892.
    KOiK01519.
    OMAiPNFPRTI.
    OrthoDBiEOG7X0VJG.
    PhylomeDBiQ9D892.
    TreeFamiTF105614.

    Enzyme and pathway databases

    ReactomeiR-MMU-15869. Metabolism of nucleotides.

    Miscellaneous databases

    NextBioi289677.
    PROiQ9D892.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9D892.
    ExpressionAtlasiQ9D892. baseline and differential.
    GenevisibleiQ9D892. MM.

    Family and domain databases

    Gene3Di3.90.950.10. 1 hit.
    HAMAPiMF_03148. HAM1_NTPase.
    InterProiIPR002637. Ham1p-like.
    IPR027502. ITPase.
    IPR029001. ITPase-like_fam.
    [Graphical view]
    PANTHERiPTHR11067. PTHR11067. 1 hit.
    PfamiPF01725. Ham1p_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF52972. SSF52972. 1 hit.
    TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Small intestine.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye.
    3. "Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase."
      Burgis N.E., Cunningham R.P.
      J. Biol. Chem. 282:3531-3538(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
    5. "ITPA protein, an enzyme that eliminates deaminated purine nucleoside triphosphates in cells."
      Sakumi K., Abolhassani N., Behmanesh M., Iyama T., Tsuchimoto D., Nakabeppu Y.
      Mutat. Res. 703:43-50(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "NUDT16 and ITPA play a dual protective role in maintaining chromosome stability and cell growth by eliminating dIDP/IDP and dITP/ITP from nucleotide pools in mammals."
      Abolhassani N., Iyama T., Tsuchimoto D., Sakumi K., Ohno M., Behmanesh M., Nakabeppu Y.
      Nucleic Acids Res. 38:2891-2903(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiITPA_MOUSE
    AccessioniPrimary (citable) accession number: Q9D892
    Secondary accession number(s): Q8R0Q8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: April 23, 2003
    Last modified: March 16, 2016
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.