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Q9D880 (TIM50_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene names
Name:Timm50
Synonyms:Tim50
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane By similarity.

Subunit structure

Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM23. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15 By similarity.

Subcellular location

Mitochondrion inner membrane; Single-pass membrane protein By similarity.

Sequence similarities

Belongs to the TIM50 family.

Contains 1 FCP1 homology domain.

Sequence caution

The sequence AAH10303.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein transport
Translocation
Transport
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainTransit peptide
Transmembrane
Transmembrane helix
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmitochondrial membrane organization

Inferred from sequence or structural similarity. Source: UniProtKB

protein dephosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

release of cytochrome c from mitochondria

Inferred from sequence alignment Ref.1. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mitochondrial inner membrane

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrial inner membrane presequence translocase complex

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion

Inferred from direct assay PubMed 18614015. Source: MGI

nuclear speck

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioninterleukin-2 receptor binding

Inferred from sequence alignment Ref.1. Source: MGI

phosphoprotein phosphatase activity

Inferred from sequence alignment Ref.1. Source: MGI

protein serine/threonine phosphatase activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein tyrosine phosphatase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ribonucleoprotein complex binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2121Mitochondrion Potential
Chain22 – 353332Mitochondrial import inner membrane translocase subunit TIM50
PRO_0000043116

Regions

Topological domain22 – 6544Mitochondrial matrix Potential
Transmembrane66 – 8621Helical; Potential
Topological domain87 – 353267Mitochondrial intermembrane Potential
Domain143 – 286144FCP1 homology

Sequences

Sequence LengthMass (Da)Tools
Q9D880 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 1BAC1FD183C532F4

FASTA35339,776
        10         20         30         40         50         60 
MAASAALFSR LRSGLRVGAR GLCTRLAPPP PRTPEQVTEI ANRGGSKAQG PQHQPGSEGP 

        70         80         90        100        110        120 
SYAKKIALWI AGLLGAGGTV SIVYIFGNNP VDENGTKIPD EFDSDPILVQ QLRRTYKYFK 

       130        140        150        160        170        180 
DYRQMIIEPT SPCLLPDPLR EPYYQPPYTL VLELTGVLLH PEWSLATGWR FKKRPGIETL 

       190        200        210        220        230        240 
FQQLAPLYEI VIFTSETGMT AFPLIDSVDP HGFISYRLFR DATRYMEGHH VKDISCLNRD 

       250        260        270        280        290        300 
PARVVVVDCK KEAFRLQPFN GVALRPWDGN SDDRVLLDLS AFLKTIALNQ VEDVRTVLEH 

       310        320        330        340        350 
YALEDDPLEA FKQRQSRLEQ EEQQRLAELS KSNRQGLSFG SLASRLWPRS KQP 

« Hide

References

« Hide 'large scale' references
[1]"Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death."
Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A., Fernandes-Alnemri T., Alnemri E.S.
J. Biol. Chem. 279:24813-24825(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Placenta and Small intestine.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-353.
Strain: FVB/N and FVB/N-3.
Tissue: Mammary gland.
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 48-64 AND 275-284, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY551342 mRNA. Translation: AAT01209.1.
AK008340 mRNA. Translation: BAB25615.1.
AK167473 mRNA. Translation: BAE39556.1.
BC010303 mRNA. Translation: AAH10303.1. Different initiation.
BC025844 mRNA. Translation: AAH25844.1.
CCDSCCDS21039.1.
RefSeqNP_079892.1. NM_025616.3.
UniGeneMm.167913.

3D structure databases

ProteinModelPortalQ9D880.
SMRQ9D880. Positions 123-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211535. 2 interactions.
IntActQ9D880. 1 interaction.
STRING10090.ENSMUSP00000080614.

PTM databases

PhosphoSiteQ9D880.

Proteomic databases

MaxQBQ9D880.
PaxDbQ9D880.
PRIDEQ9D880.

Protocols and materials databases

DNASU66525.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081946; ENSMUSP00000080614; ENSMUSG00000003438.
GeneID66525.
KEGGmmu:66525.
UCSCuc009fyg.1. mouse.

Organism-specific databases

CTD92609.
MGIMGI:1913775. Timm50.

Phylogenomic databases

eggNOGCOG5190.
GeneTreeENSGT00550000075036.
HOGENOMHOG000044824.
HOVERGENHBG084157.
InParanoidQ9D880.
KOK17496.
OMAMEGHHVK.
OrthoDBEOG773XH7.
PhylomeDBQ9D880.
TreeFamTF106198.

Gene expression databases

BgeeQ9D880.
GenevestigatorQ9D880.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR023214. HAD-like_dom.
IPR004274. NIF.
IPR027111. Tim50.
[Graphical view]
PANTHERPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamPF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
PROSITEPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTIMM50. mouse.
NextBio321936.
PROQ9D880.
SOURCESearch...

Entry information

Entry nameTIM50_MOUSE
AccessionPrimary (citable) accession number: Q9D880
Secondary accession number(s): Q8R3A7, Q91Z17
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot