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Q9D880

- TIM50_MOUSE

UniProt

Q9D880 - TIM50_MOUSE

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Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

Timm50

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.By similarity

GO - Molecular functioni

  1. interleukin-2 receptor binding Source: MGI
  2. phosphoprotein phosphatase activity Source: MGI
  3. protein serine/threonine phosphatase activity Source: UniProtKB
  4. protein tyrosine phosphatase activity Source: UniProtKB
  5. ribonucleoprotein complex binding Source: Ensembl

GO - Biological processi

  1. mitochondrial membrane organization Source: UniProtKB
  2. protein dephosphorylation Source: UniProtKB
  3. protein transport Source: UniProtKB-KW
  4. release of cytochrome c from mitochondria Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiREACT_199101. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:Timm50
Synonyms:Tim50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1913775. Timm50.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB
  3. mitochondrial inner membrane presequence translocase complex Source: UniProtKB
  4. mitochondrion Source: MGI
  5. nuclear speck Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2121MitochondrionSequence AnalysisAdd
BLAST
Chaini22 – 353332Mitochondrial import inner membrane translocase subunit TIM50PRO_0000043116Add
BLAST

Proteomic databases

MaxQBiQ9D880.
PaxDbiQ9D880.
PRIDEiQ9D880.

PTM databases

PhosphoSiteiQ9D880.

Expressioni

Gene expression databases

BgeeiQ9D880.
GenevestigatoriQ9D880.

Interactioni

Subunit structurei

Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM23. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15 (By similarity).By similarity

Protein-protein interaction databases

BioGridi211535. 2 interactions.
IntActiQ9D880. 1 interaction.
STRINGi10090.ENSMUSP00000080614.

Structurei

3D structure databases

ProteinModelPortaliQ9D880.
SMRiQ9D880. Positions 123-314.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 6544Mitochondrial matrixSequence AnalysisAdd
BLAST
Topological domaini87 – 353267Mitochondrial intermembraneSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei66 – 8621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 286144FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TIM50 family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00550000075036.
HOGENOMiHOG000044824.
HOVERGENiHBG084157.
InParanoidiQ9D880.
KOiK17496.
OMAiMEGHHVK.
OrthoDBiEOG773XH7.
PhylomeDBiQ9D880.
TreeFamiTF106198.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR004274. NIF.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D880-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAASAALFSR LRSGLRVGAR GLCTRLAPPP PRTPEQVTEI ANRGGSKAQG
60 70 80 90 100
PQHQPGSEGP SYAKKIALWI AGLLGAGGTV SIVYIFGNNP VDENGTKIPD
110 120 130 140 150
EFDSDPILVQ QLRRTYKYFK DYRQMIIEPT SPCLLPDPLR EPYYQPPYTL
160 170 180 190 200
VLELTGVLLH PEWSLATGWR FKKRPGIETL FQQLAPLYEI VIFTSETGMT
210 220 230 240 250
AFPLIDSVDP HGFISYRLFR DATRYMEGHH VKDISCLNRD PARVVVVDCK
260 270 280 290 300
KEAFRLQPFN GVALRPWDGN SDDRVLLDLS AFLKTIALNQ VEDVRTVLEH
310 320 330 340 350
YALEDDPLEA FKQRQSRLEQ EEQQRLAELS KSNRQGLSFG SLASRLWPRS

KQP
Length:353
Mass (Da):39,776
Last modified:June 1, 2001 - v1
Checksum:i1BAC1FD183C532F4
GO

Sequence cautioni

The sequence AAH10303.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY551342 mRNA. Translation: AAT01209.1.
AK008340 mRNA. Translation: BAB25615.1.
AK167473 mRNA. Translation: BAE39556.1.
BC010303 mRNA. Translation: AAH10303.1. Different initiation.
BC025844 mRNA. Translation: AAH25844.1.
CCDSiCCDS21039.1.
RefSeqiNP_079892.1. NM_025616.3.
UniGeneiMm.167913.

Genome annotation databases

EnsembliENSMUST00000081946; ENSMUSP00000080614; ENSMUSG00000003438.
GeneIDi66525.
KEGGimmu:66525.
UCSCiuc009fyg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY551342 mRNA. Translation: AAT01209.1 .
AK008340 mRNA. Translation: BAB25615.1 .
AK167473 mRNA. Translation: BAE39556.1 .
BC010303 mRNA. Translation: AAH10303.1 . Different initiation.
BC025844 mRNA. Translation: AAH25844.1 .
CCDSi CCDS21039.1.
RefSeqi NP_079892.1. NM_025616.3.
UniGenei Mm.167913.

3D structure databases

ProteinModelPortali Q9D880.
SMRi Q9D880. Positions 123-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211535. 2 interactions.
IntActi Q9D880. 1 interaction.
STRINGi 10090.ENSMUSP00000080614.

PTM databases

PhosphoSitei Q9D880.

Proteomic databases

MaxQBi Q9D880.
PaxDbi Q9D880.
PRIDEi Q9D880.

Protocols and materials databases

DNASUi 66525.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081946 ; ENSMUSP00000080614 ; ENSMUSG00000003438 .
GeneIDi 66525.
KEGGi mmu:66525.
UCSCi uc009fyg.1. mouse.

Organism-specific databases

CTDi 92609.
MGIi MGI:1913775. Timm50.

Phylogenomic databases

eggNOGi COG5190.
GeneTreei ENSGT00550000075036.
HOGENOMi HOG000044824.
HOVERGENi HBG084157.
InParanoidi Q9D880.
KOi K17496.
OMAi MEGHHVK.
OrthoDBi EOG773XH7.
PhylomeDBi Q9D880.
TreeFami TF106198.

Enzyme and pathway databases

Reactomei REACT_199101. Mitochondrial protein import.

Miscellaneous databases

ChiTaRSi TIMM50. mouse.
NextBioi 321936.
PROi Q9D880.
SOURCEi Search...

Gene expression databases

Bgeei Q9D880.
Genevestigatori Q9D880.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR023214. HAD-like_dom.
IPR004274. NIF.
IPR027111. Tim50.
[Graphical view ]
PANTHERi PTHR12210:SF3. PTHR12210:SF3. 1 hit.
Pfami PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00577. CPDc. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
PROSITEi PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death."
    Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A., Fernandes-Alnemri T., Alnemri E.S.
    J. Biol. Chem. 279:24813-24825(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Placenta and Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-353.
    Strain: FVB/N and FVB/N-3.
    Tissue: Mammary gland.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 48-64 AND 275-284, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiTIM50_MOUSE
AccessioniPrimary (citable) accession number: Q9D880
Secondary accession number(s): Q8R3A7, Q91Z17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2001
Last modified: October 1, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3