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Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

Timm50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:Timm50
Synonyms:Tim50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913775. Timm50.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 65Mitochondrial matrixSequence analysisAdd BLAST44
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Topological domaini87 – 353Mitochondrial intermembraneSequence analysisAdd BLAST267

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 21MitochondrionSequence analysisAdd BLAST21
ChainiPRO_000004311622 – 353Mitochondrial import inner membrane translocase subunit TIM50Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D880.
MaxQBiQ9D880.
PaxDbiQ9D880.
PeptideAtlasiQ9D880.
PRIDEiQ9D880.

PTM databases

iPTMnetiQ9D880.
PhosphoSitePlusiQ9D880.
SwissPalmiQ9D880.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003438.
GenevisibleiQ9D880. MM.

Interactioni

Subunit structurei

Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM23. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211535. 2 interactors.
IntActiQ9D880. 1 interactor.
STRINGi10090.ENSMUSP00000080614.

Structurei

3D structure databases

ProteinModelPortaliQ9D880.
SMRiQ9D880.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 286FCP1 homologyPROSITE-ProRule annotationAdd BLAST144

Sequence similaritiesi

Belongs to the TIM50 family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2832. Eukaryota.
COG5190. LUCA.
GeneTreeiENSGT00550000075036.
HOGENOMiHOG000044824.
HOVERGENiHBG084157.
InParanoidiQ9D880.
KOiK17496.
OMAiMEGHHVK.
OrthoDBiEOG091G0H62.
PhylomeDBiQ9D880.
TreeFamiTF106198.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASAALFSR LRSGLRVGAR GLCTRLAPPP PRTPEQVTEI ANRGGSKAQG
60 70 80 90 100
PQHQPGSEGP SYAKKIALWI AGLLGAGGTV SIVYIFGNNP VDENGTKIPD
110 120 130 140 150
EFDSDPILVQ QLRRTYKYFK DYRQMIIEPT SPCLLPDPLR EPYYQPPYTL
160 170 180 190 200
VLELTGVLLH PEWSLATGWR FKKRPGIETL FQQLAPLYEI VIFTSETGMT
210 220 230 240 250
AFPLIDSVDP HGFISYRLFR DATRYMEGHH VKDISCLNRD PARVVVVDCK
260 270 280 290 300
KEAFRLQPFN GVALRPWDGN SDDRVLLDLS AFLKTIALNQ VEDVRTVLEH
310 320 330 340 350
YALEDDPLEA FKQRQSRLEQ EEQQRLAELS KSNRQGLSFG SLASRLWPRS

KQP
Length:353
Mass (Da):39,776
Last modified:June 1, 2001 - v1
Checksum:i1BAC1FD183C532F4
GO

Sequence cautioni

The sequence AAH10303 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY551342 mRNA. Translation: AAT01209.1.
AK008340 mRNA. Translation: BAB25615.1.
AK167473 mRNA. Translation: BAE39556.1.
BC010303 mRNA. Translation: AAH10303.1. Different initiation.
BC025844 mRNA. Translation: AAH25844.1.
CCDSiCCDS21039.1.
RefSeqiNP_079892.1. NM_025616.3.
UniGeneiMm.167913.

Genome annotation databases

EnsembliENSMUST00000081946; ENSMUSP00000080614; ENSMUSG00000003438.
GeneIDi66525.
KEGGimmu:66525.
UCSCiuc009fyg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY551342 mRNA. Translation: AAT01209.1.
AK008340 mRNA. Translation: BAB25615.1.
AK167473 mRNA. Translation: BAE39556.1.
BC010303 mRNA. Translation: AAH10303.1. Different initiation.
BC025844 mRNA. Translation: AAH25844.1.
CCDSiCCDS21039.1.
RefSeqiNP_079892.1. NM_025616.3.
UniGeneiMm.167913.

3D structure databases

ProteinModelPortaliQ9D880.
SMRiQ9D880.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211535. 2 interactors.
IntActiQ9D880. 1 interactor.
STRINGi10090.ENSMUSP00000080614.

PTM databases

iPTMnetiQ9D880.
PhosphoSitePlusiQ9D880.
SwissPalmiQ9D880.

Proteomic databases

EPDiQ9D880.
MaxQBiQ9D880.
PaxDbiQ9D880.
PeptideAtlasiQ9D880.
PRIDEiQ9D880.

Protocols and materials databases

DNASUi66525.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081946; ENSMUSP00000080614; ENSMUSG00000003438.
GeneIDi66525.
KEGGimmu:66525.
UCSCiuc009fyg.1. mouse.

Organism-specific databases

CTDi92609.
MGIiMGI:1913775. Timm50.

Phylogenomic databases

eggNOGiKOG2832. Eukaryota.
COG5190. LUCA.
GeneTreeiENSGT00550000075036.
HOGENOMiHOG000044824.
HOVERGENiHBG084157.
InParanoidiQ9D880.
KOiK17496.
OMAiMEGHHVK.
OrthoDBiEOG091G0H62.
PhylomeDBiQ9D880.
TreeFamiTF106198.

Miscellaneous databases

ChiTaRSiTimm50. mouse.
PROiQ9D880.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003438.
GenevisibleiQ9D880. MM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIM50_MOUSE
AccessioniPrimary (citable) accession number: Q9D880
Secondary accession number(s): Q8R3A7, Q91Z17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.