Q9D880 (TIM50_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50

Gene names

Name:	Timm50
Synonyms:	Tim50

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane By similarity.
Subunit structure	Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50. Interacts directly with TIMM23 By similarity.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the TIM50 family. Contains 1 FCP1 homology domain.
Sequence caution	The sequence AAH10303.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial membrane organization Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW release of cytochrome c from mitochondria Inferred from sequence alignment Ref.1. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane presequence translocase complex Inferred from sequence or structural similarity. Source: UniProtKB nuclear speck Inferred from electronic annotation. Source: Compara
Molecular_function	interleukin-2 receptor binding Inferred from sequence alignment Ref.1. Source: MGI protein serine/threonine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB protein tyrosine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB ribonucleoprotein complex binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 21	21	Mitochondrion Potential
	Chain	22 – 353	332	Mitochondrial import inner membrane translocase subunit TIM50		PRO_0000043116
Regions
	Topological domain	22 – 65	44	Mitochondrial matrix Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Topological domain	87 – 353	267	Mitochondrial intermembrane Potential
	Domain	143 – 286	144	FCP1 homology

Sequences

	Sequence		Length	Mass (Da)
	Q9D880 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 1BAC1FD183C532F4	FASTA	353	39,776
	10 20 30 40 50 60 MAASAALFSR LRSGLRVGAR GLCTRLAPPP PRTPEQVTEI ANRGGSKAQG PQHQPGSEGP 70 80 90 100 110 120 SYAKKIALWI AGLLGAGGTV SIVYIFGNNP VDENGTKIPD EFDSDPILVQ QLRRTYKYFK 130 140 150 160 170 180 DYRQMIIEPT SPCLLPDPLR EPYYQPPYTL VLELTGVLLH PEWSLATGWR FKKRPGIETL 190 200 210 220 230 240 FQQLAPLYEI VIFTSETGMT AFPLIDSVDP HGFISYRLFR DATRYMEGHH VKDISCLNRD 250 260 270 280 290 300 PARVVVVDCK KEAFRLQPFN GVALRPWDGN SDDRVLLDLS AFLKTIALNQ VEDVRTVLEH 310 320 330 340 350 YALEDDPLEA FKQRQSRLEQ EEQQRLAELS KSNRQGLSFG SLASRLWPRS KQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death." Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A., Fernandes-Alnemri T., Alnemri E.S. J. Biol. Chem. 279:24813-24825(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Placenta and Small intestine.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-353. Strain: FVB/N and FVB/N-3. Tissue: Mammary gland.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 48-64 AND 275-284, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY551342 mRNA. Translation: AAT01209.1. AK008340 mRNA. Translation: BAB25615.1. AK167473 mRNA. Translation: BAE39556.1. BC010303 mRNA. Translation: AAH10303.1. Different initiation. BC025844 mRNA. Translation: AAH25844.1.
IPI	IPI00111045.
RefSeq	NP_079892.1. NM_025616.3.
UniGene	Mm.167913.
3D structure databases
ProteinModelPortal	Q9D880.
SMR	Q9D880. Positions 123-314.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9D880. 1 interaction.
STRING	10090.ENSMUSP00000080614.
PTM databases
PhosphoSite	Q9D880.
Proteomic databases
PaxDb	Q9D880.
PRIDE	Q9D880.
Protocols and materials databases
DNASU	66525.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000081946; ENSMUSP00000080614; ENSMUSG00000003438.
GeneID	66525.
KEGG	mmu:66525.
UCSC	uc009fyg.1. mouse.
Organism-specific databases
CTD	92609.
MGI	MGI:1913775. Timm50.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00550000075036.
HOGENOM	HOG000044824.
HOVERGEN	HBG084157.
InParanoid	Q9D880.
OMA	MEGHHVK.
OrthoDB	EOG42RD84.
Gene expression databases
Bgee	Q9D880.
Genevestigator	Q9D880.
GermOnline	ENSMUSG00000003438. Mus musculus.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR023214. HAD-like_dom. IPR004274. NIF. IPR027111. Tim50. [Graphical view]
PANTHER	PTHR12210:SF3. PTHR12210:SF3. 1 hit.
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TIMM50. mouse.
NextBio	321936.
SOURCE	Search...

Entry information

Entry name

TIM50_MOUSE

Accession

Primary (citable) accession number: Q9D880
Secondary accession number(s): Q8R3A7, Q91Z17

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program