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Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

Timm50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:Timm50
Synonyms:Tim50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913775 Timm50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 65Mitochondrial matrixSequence analysisAdd BLAST44
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Topological domaini87 – 353Mitochondrial intermembraneSequence analysisAdd BLAST267

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 21MitochondrionSequence analysisAdd BLAST21
ChainiPRO_000004311622 – 353Mitochondrial import inner membrane translocase subunit TIM50Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D880
MaxQBiQ9D880
PaxDbiQ9D880
PeptideAtlasiQ9D880
PRIDEiQ9D880

PTM databases

iPTMnetiQ9D880
PhosphoSitePlusiQ9D880
SwissPalmiQ9D880

Expressioni

Gene expression databases

BgeeiENSMUSG00000003438
GenevisibleiQ9D880 MM

Interactioni

Subunit structurei

Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM23. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211535, 3 interactors
IntActiQ9D880, 1 interactor
STRINGi10090.ENSMUSP00000080614

Structurei

3D structure databases

ProteinModelPortaliQ9D880
SMRiQ9D880
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 286FCP1 homologyPROSITE-ProRule annotationAdd BLAST144

Sequence similaritiesi

Belongs to the TIM50 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2832 Eukaryota
COG5190 LUCA
GeneTreeiENSGT00550000075036
HOGENOMiHOG000044824
HOVERGENiHBG084157
InParanoidiQ9D880
KOiK17496
OMAiRMKLGFA
OrthoDBiEOG091G0H62
PhylomeDBiQ9D880
TreeFamiTF106198

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR004274 FCP1_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR027111 Tim50
PANTHERiPTHR12210:SF3 PTHR12210:SF3, 1 hit
PfamiView protein in Pfam
PF03031 NIF, 1 hit
SMARTiView protein in SMART
SM00577 CPDc, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
PROSITEiView protein in PROSITE
PS50969 FCP1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASAALFSR LRSGLRVGAR GLCTRLAPPP PRTPEQVTEI ANRGGSKAQG
60 70 80 90 100
PQHQPGSEGP SYAKKIALWI AGLLGAGGTV SIVYIFGNNP VDENGTKIPD
110 120 130 140 150
EFDSDPILVQ QLRRTYKYFK DYRQMIIEPT SPCLLPDPLR EPYYQPPYTL
160 170 180 190 200
VLELTGVLLH PEWSLATGWR FKKRPGIETL FQQLAPLYEI VIFTSETGMT
210 220 230 240 250
AFPLIDSVDP HGFISYRLFR DATRYMEGHH VKDISCLNRD PARVVVVDCK
260 270 280 290 300
KEAFRLQPFN GVALRPWDGN SDDRVLLDLS AFLKTIALNQ VEDVRTVLEH
310 320 330 340 350
YALEDDPLEA FKQRQSRLEQ EEQQRLAELS KSNRQGLSFG SLASRLWPRS

KQP
Length:353
Mass (Da):39,776
Last modified:June 1, 2001 - v1
Checksum:i1BAC1FD183C532F4
GO

Sequence cautioni

The sequence AAH10303 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY551342 mRNA Translation: AAT01209.1
AK008340 mRNA Translation: BAB25615.1
AK167473 mRNA Translation: BAE39556.1
BC010303 mRNA Translation: AAH10303.1 Different initiation.
BC025844 mRNA Translation: AAH25844.1
CCDSiCCDS21039.1
RefSeqiNP_079892.1, NM_025616.3
UniGeneiMm.167913

Genome annotation databases

EnsembliENSMUST00000081946; ENSMUSP00000080614; ENSMUSG00000003438
GeneIDi66525
KEGGimmu:66525
UCSCiuc009fyg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTIM50_MOUSE
AccessioniPrimary (citable) accession number: Q9D880
Secondary accession number(s): Q8R3A7, Q91Z17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health