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Protein

Pre-mRNA 3'-end-processing factor FIP1

Gene

Fip1l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-77595 Processing of Intronless Pre-mRNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA 3'-end-processing factor FIP1
Alternative name(s):
FIP1-like 1 protein
Gene namesi
Name:Fip1l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1914149 Fip1l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002150381 – 581Pre-mRNA 3'-end-processing factor FIP1Add BLAST581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84PhosphoserineCombined sources1
Modified residuei86PhosphoserineCombined sources1
Modified residuei88PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei411PhosphotyrosineBy similarity1
Modified residuei479PhosphoserineCombined sources1
Modified residuei481PhosphothreonineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei487PhosphoserineCombined sources1
Modified residuei541PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D824
PeptideAtlasiQ9D824
PRIDEiQ9D824

PTM databases

iPTMnetiQ9D824
PhosphoSitePlusiQ9D824

Expressioni

Gene expression databases

BgeeiENSMUSG00000029227
CleanExiMM_FIP1L1
ExpressionAtlasiQ9D824 baseline and differential
GenevisibleiQ9D824 MM

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with CPSF1, CPSF4, CSTF2 and CSTF3 (By similarity). Interacts with AHCYL1 (when phosphorylated); the interaction is direct and associates AHCYL1 with the CPSF complex and RNA (PubMed:19224921). Interacts with PAPOLA; the interaction seems to be increased by the interaction with AHCYL1 (PubMed:19224921). Interacts with NUDT21/CPSF5; this interaction occurs in a RNA sequence-specific manner. Interacts (preferentially via unphosphorylated form and Arg/Glu/Asp-rich domain) with CPSF6 (via Arg/Ser-rich domain); this interaction mediates, at least in part, the interaction between the CFIm and CPSF complexes and may be inhibited by CPSF6 hyper-phosphorylation. Interacts (preferentially via unphosphorylated form and Arg/Asp/Glu-rich domain) with CPSF7 (via Arg/Ser-rich domain); this interaction mediates, at least in part, the interaction between the CFIm and CPSF complexes and may be inhibited by CPSF7 hyper-phosphorylation (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi211797, 1 interactor
IntActiQ9D824, 3 interactors
MINTiQ9D824
STRINGi10090.ENSMUSP00000109164

Structurei

3D structure databases

ProteinModelPortaliQ9D824
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 332Necessary for stimulating PAPOLA activityBy similarityAdd BLAST332
Regioni1 – 110Sufficient for interaction with PAPOLABy similarityAdd BLAST110
Regioni136 – 219Sufficient for interaction with CPSF4By similarityAdd BLAST84
Regioni428 – 581Sufficient for interaction with CPSF1 and CSTF3By similarityAdd BLAST154
Regioni442 – 477Arg/Asp/Glu-rich domainBy similarityAdd BLAST36
Regioni478 – 535Sufficient for interaction with AHCYL11 PublicationAdd BLAST58

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi332 – 391Pro-richAdd BLAST60
Compositional biasi441 – 549Arg-richAdd BLAST109
Compositional biasi457 – 581Glu-richAdd BLAST125

Sequence similaritiesi

Belongs to the FIP1 family.Curated

Phylogenomic databases

eggNOGiKOG1049 Eukaryota
COG5213 LUCA
GeneTreeiENSGT00730000111028
HOGENOMiHOG000004854
HOVERGENiHBG059889
InParanoidiQ9D824
KOiK14405
OMAiGNNIQVI
PhylomeDBiQ9D824
TreeFamiTF318610

Family and domain databases

InterProiView protein in InterPro
IPR007854 Fip1
PfamiView protein in Pfam
PF05182 Fip1, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D824-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAGEVERLV ELSGGTGGDE EEEWLYGGPW DVHVHSDLAK DLDENEVERP
60 70 80 90 100
EEENASANPP SGIEEEAAEN GVAKPKVTET EDDSDSDSDD DEDDVHVTIG
110 120 130 140 150
DIKTGAPQYG SYGTAPVNLN IKAGGRVYGN TGTKVKGVDL DAPGSINGVP
160 170 180 190 200
LLEVDLDSFE DKPWRKPGAD LSDYFNYGFN EDTWKAYCEK QKRIRMGLEV
210 220 230 240 250
IPVTSTTNKI TVQQGRTGNS EKEAALPSTK AEFTSPPSLF KTGLPPSRNS
260 270 280 290 300
TSSQSQTSTA SRKASSSVGK WQDRYGRAES PDLRRLPGAI DVIGQTITIS
310 320 330 340 350
RVEGRRRANE NSNIQVLSDR SATEVDNNFS KPPPFFPPGA PPTHLPPPPF
360 370 380 390 400
LPPPPTVSTA PPLIPPPGIP ITVPPPGFPP PPGAPPPSLI PTIESGHSSG
410 420 430 440 450
YDSRSARAFP YGNVAFPHLT SSAPSWPSLV DTTKQWDYYA RREKDRDRDR
460 470 480 490 500
ERDRDRERER DRDRERERTR ERERERDHSP TPSVFNSDEE RYRYREYAER
510 520 530 540 550
GYERHRASRE KEERHRERRH REKEETRHKS SRSNSRRRHE SEEGDSHRRH
560 570 580
KHKKSKRSKE GKEAGSEPVP EQESTEAAPA E
Length:581
Mass (Da):64,959
Last modified:June 1, 2001 - v1
Checksum:i2E8D6DF558168035
GO
Isoform 2 (identifier: Q9D824-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-283: Missing.

Show »
Length:545
Mass (Da):61,060
Checksum:iD8C65A7E20CA0985
GO
Isoform 3 (identifier: Q9D824-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-283: Missing.
     365-373: Missing.

Show »
Length:536
Mass (Da):60,188
Checksum:iB608BE48974164C0
GO
Isoform 4 (identifier: Q9D824-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-211: T → TAEDCTMEVTPGAEIQDGRFNLFK
     365-373: Missing.
     487-581: SDEERYRYRE...QESTEAAPAE → RFVGCAGP

Show »
Length:508
Mass (Da):55,796
Checksum:i01BF436596282ED4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016733211T → TAEDCTMEVTPGAEIQDGRF NLFK in isoform 4. 1 Publication1
Alternative sequenceiVSP_016734248 – 283Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_016735365 – 373Missing in isoform 3 and isoform 4. 2 Publications9
Alternative sequenceiVSP_016736487 – 581SDEER…AAPAE → RFVGCAGP in isoform 4. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005061 mRNA Translation: BAB23785.1
AK008561 mRNA Translation: BAB25745.1
AK049672 mRNA Translation: BAC33867.1
AK164105 mRNA Translation: BAE37629.1
BC003263 mRNA Translation: AAH03263.1
CCDSiCCDS19346.1 [Q9D824-2]
CCDS51520.1 [Q9D824-1]
CCDS51521.1 [Q9D824-3]
RefSeqiNP_001153045.1, NM_001159573.1 [Q9D824-1]
NP_001153046.1, NM_001159574.1 [Q9D824-3]
NP_077145.2, NM_024183.5 [Q9D824-2]
UniGeneiMm.272468

Genome annotation databases

EnsembliENSMUST00000080164; ENSMUSP00000079059; ENSMUSG00000029227 [Q9D824-2]
ENSMUST00000113534; ENSMUSP00000109162; ENSMUSG00000029227 [Q9D824-4]
ENSMUST00000113535; ENSMUSP00000109163; ENSMUSG00000029227 [Q9D824-3]
ENSMUST00000113536; ENSMUSP00000109164; ENSMUSG00000029227 [Q9D824-1]
GeneIDi66899
KEGGimmu:66899
UCSCiuc008xtm.2 mouse [Q9D824-4]
uc008xtn.2 mouse [Q9D824-2]
uc008xto.2 mouse [Q9D824-1]
uc008xtp.2 mouse [Q9D824-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFIP1_MOUSE
AccessioniPrimary (citable) accession number: Q9D824
Secondary accession number(s): Q8BWX7, Q99LH0, Q9DBB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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