Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9D7Z6

- CLCA1_MOUSE

UniProt

Q9D7Z6 - CLCA1_MOUSE

Protein

Calcium-activated chloride channel regulator 1

Gene

Clca1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (01 Dec 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.3 Publications

    GO - Molecular functioni

    1. chloride channel activity Source: MGI

    GO - Biological processi

    1. calcium ion transport Source: UniProtKB-KW
    2. cellular response to hypoxia Source: Ensembl
    3. chloride transmembrane transport Source: GOC
    4. chloride transport Source: MGI

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Chloride

    Protein family/group databases

    MEROPSiM87.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-activated chloride channel regulator 1
    Alternative name(s):
    Calcium-activated chloride channel family member 3
    Short name:
    mCLCA3
    Protein gob-5
    Gene namesi
    Name:Clca1
    Synonyms:Clca3, Gob5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1346342. Clca3.

    Subcellular locationi

    Secretedextracellular space 3 Publications
    Note: The 75 kDa N-terminal and a 35 kDa C-terminal products are fully secreted into extracellular environment as a soluble complex of two glycoproteins.

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    2. integral component of membrane Source: MGI
    3. microvillus Source: MGI
    4. secretory granule Source: MGI
    5. zymogen granule membrane Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Mice exhibit significantly increased bronchoalveolar lavage (BAL) inflammation consisted predominantly of neutrophils; have decreased goblet cell hyperplasia as well as decreased mucus production; have decreased airway hypersensitivity after cholinergic provocation with methacholine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 913892Calcium-activated chloride channel regulator 1PRO_0000333692Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi504 – 5041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi770 – 7701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi804 – 8041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi810 – 8101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi836 – 8361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi887 – 8871N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The 110 kDa translation product is cleaved in the endoplasmic reticulum into a 75 kDa N-terminal and a 35 kDa C-terminal products that remain physically associated with each other.1 Publication
    Glycosylated.2 Publications

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9D7Z6.
    PaxDbiQ9D7Z6.
    PRIDEiQ9D7Z6.

    PTM databases

    PhosphoSiteiQ9D7Z6.

    Expressioni

    Tissue specificityi

    Exclusively expressed in the digestive and respiratory tracts and in the uterus (at protein level). Expressed in small intestine, colon, stomach, and uterus and slightly expressed in trachea tissue. Exclusively expressed in the mucin granule membranes of gastrointestinal, respiratory, and uterine goblet cells and other mucin-producing cells. In the colon, expressed in the surface mucous cells. In the stomach highly expressed in the surface epithelium in the pylorus. Strongly expressed in the airway epithelium of lung tissues associated with airway hyperresponsiveness (AHR).3 Publications

    Inductioni

    By IL3, IL4 and IL9 in the lung. Increases in the bronchiolar epithelium of asbestos-induced fibrogenesis. Decreases in cystic fibrosis knockout mice.3 Publications

    Gene expression databases

    BgeeiQ9D7Z6.
    GenevestigatoriQ9D7Z6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D7Z6.
    SMRiQ9D7Z6. Positions 305-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini307 – 476170VWFAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CLCR family.Curated
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG42884.
    GeneTreeiENSGT00390000004810.
    HOGENOMiHOG000015107.
    HOVERGENiHBG005560.
    InParanoidiQ9D7Z6.
    KOiK05027.
    OMAiEPYTEQM.
    OrthoDBiEOG78WKR1.
    PhylomeDBiQ9D7Z6.
    TreeFamiTF328396.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR004727. CaCC_prot.
    IPR013642. Cl_channel_Ca.
    IPR015394. DUF1973.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF08434. CLCA_N. 1 hit.
    PF09315. DUF1973. 1 hit.
    [Graphical view]
    SMARTiSM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    TIGRFAMsiTIGR00868. hCaCC. 1 hit.
    PROSITEiPS50234. VWFA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D7Z6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLKSPVFL LILHLLEGVL SESLIQLNNN GYEGIVIAID HDVPEDEALI    50
    QHIKDMVTQA SPYLFEATGK RFYFKNVAIL IPESWKAKPE YTRPKLETFK 100
    NADVLVSTTS PLGNDEPYTE HIGACGEKGI RIHLTPDFLA GKKLTQYGPQ 150
    DRTFVHEWAH FRWGVFNEYN NDEKFYLSKG KPQAVRCSAA ITGKNQVRRC 200
    QGGSCITNGK CVIDRVTGLY KDNCVFVPDP HQNEKASIMF NQNINSVVEF 250
    CTEKNHNQEA PNDQNQRCNL RSTWEVIQES EDFKQTTPMT AQPPAPTFSL 300
    LQIGQRIVCL VLDKSGSMLN DDRLNRMNQA SRLFLLQTVE QGSWVGMVTF 350
    DSAAYVQSEL KQLNSGADRD LLIKHLPTVS AGGTSICSGL RTAFTVIKKK 400
    YPTDGSEIVL LTDGEDNTIS SCFDLVKQSG AIIHTVALGP AAAKELEQLS 450
    KMTGGLQTYS SDQVQNNGLV DAFAALSSGN AAIAQHSIQL ESRGVNLQNN 500
    QWMNGSVIVD SSVGKDTLFL ITWTTHPPTI FIWDPSGVEQ NGFILDTTTK 550
    VAYLQVPGTA KVGFWKYSIQ ASSQTLTLTV TSRAASATLP PITVTPVVNK 600
    NTGKFPSPVT VYASIRQGAS PILRASVTAL IESVNGKTVT LELLDNGAGA 650
    DATKNDGVYS RFFTAFDANG RYSVKIWALG GVTSDRQRAA PPKNRAMYID 700
    GWIEDGEVRM NPPRPETSYV QDKQLCFSRT SSGGSFVATN VPAAAPIPDL 750
    FPPCQITDLK ASIQGQNLVN LTWTAPGDDY DHGRASNYII RMSTSIVDLR 800
    DHFNTSLQVN TTGLIPKEAS SEEIFEFELG GNTFGNGTDI FIAIQAVDKS 850
    NLKSEISNIA RVSVFIPAQE PPIPEDSTPP CPDISINSTI PGIHVLKIMW 900
    KWLGEMQVTL GLH 913
    Length:913
    Mass (Da):100,071
    Last modified:December 1, 2001 - v2
    Checksum:iA7FA2F9E1089806D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti469 – 4691L → F in AAH28343. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017156 mRNA. Translation: BAA33743.1.
    AK008659 mRNA. Translation: BAB25815.2.
    AK136476 mRNA. Translation: BAE22995.1.
    BC028343 mRNA. Translation: AAH28343.1.
    BC116318 mRNA. Translation: AAI16319.1.
    BC116319 mRNA. Translation: AAI16320.1.
    CCDSiCCDS17889.1.
    PIRiJG0168.
    RefSeqiNP_059502.1. NM_017474.2.
    UniGeneiMm.33483.

    Genome annotation databases

    EnsembliENSMUST00000029919; ENSMUSP00000029919; ENSMUSG00000028255.
    GeneIDi23844.
    KEGGimmu:23844.
    UCSCiuc008rqf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017156 mRNA. Translation: BAA33743.1 .
    AK008659 mRNA. Translation: BAB25815.2 .
    AK136476 mRNA. Translation: BAE22995.1 .
    BC028343 mRNA. Translation: AAH28343.1 .
    BC116318 mRNA. Translation: AAI16319.1 .
    BC116319 mRNA. Translation: AAI16320.1 .
    CCDSi CCDS17889.1.
    PIRi JG0168.
    RefSeqi NP_059502.1. NM_017474.2.
    UniGenei Mm.33483.

    3D structure databases

    ProteinModelPortali Q9D7Z6.
    SMRi Q9D7Z6. Positions 305-477.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M87.001.

    PTM databases

    PhosphoSitei Q9D7Z6.

    Proteomic databases

    MaxQBi Q9D7Z6.
    PaxDbi Q9D7Z6.
    PRIDEi Q9D7Z6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029919 ; ENSMUSP00000029919 ; ENSMUSG00000028255 .
    GeneIDi 23844.
    KEGGi mmu:23844.
    UCSCi uc008rqf.2. mouse.

    Organism-specific databases

    CTDi 23844.
    MGIi MGI:1346342. Clca3.

    Phylogenomic databases

    eggNOGi NOG42884.
    GeneTreei ENSGT00390000004810.
    HOGENOMi HOG000015107.
    HOVERGENi HBG005560.
    InParanoidi Q9D7Z6.
    KOi K05027.
    OMAi EPYTEQM.
    OrthoDBi EOG78WKR1.
    PhylomeDBi Q9D7Z6.
    TreeFami TF328396.

    Miscellaneous databases

    NextBioi 303523.
    PROi Q9D7Z6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D7Z6.
    Genevestigatori Q9D7Z6.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR004727. CaCC_prot.
    IPR013642. Cl_channel_Ca.
    IPR015394. DUF1973.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF08434. CLCA_N. 1 hit.
    PF09315. DUF1973. 1 hit.
    [Graphical view ]
    SMARTi SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    TIGRFAMsi TIGR00868. hCaCC. 1 hit.
    PROSITEi PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and identification of the gene gob-5, which is expressed in intestinal goblet cells in mice."
      Komiya T., Tanigawa Y., Hirohashi S.
      Biochem. Biophys. Res. Commun. 255:347-351(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Intestine.
    2. "Characterization of a calcium-activated chloride channel as a shared target of Th2 cytokine pathways and its potential involvement in asthma."
      Zhou Y., Dong Q., Louahed J., Dragwa C., Savio D., Huang M., Weiss C., Tomer Y., McLane M.P., Nicolaides N.C., Levitt R.C.
      Am. J. Respir. Cell Mol. Biol. 25:486-491(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Colon and Stomach.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    5. Cited for: FUNCTION, TISSUE SPECIFICITY.
    6. "The murine mCLCA3 (alias gob-5) protein is located in the mucin granule membranes of intestinal, respiratory, and uterine goblet cells."
      Leverkoehne I., Gruber A.D.
      J. Histochem. Cytochem. 50:829-838(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PROTEOLYTIC PROCESSING, GLYCOSYLATION.
    7. "Gene expression profiles reveal increased mClca3 (Gob5) expression and mucin production in a murine model of asbestos-induced fibrogenesis."
      Sabo-Attwood T., Ramos-Nino M., Bond J., Butnor K.J., Heintz N., Gruber A.D., Steele C., Taatjes D.J., Vacek P., Mossman B.T.
      Am. J. Pathol. 167:1243-1256(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels."
      Gibson A., Lewis A.P., Affleck K., Aitken A.J., Meldrum E., Thompson N.
      J. Biol. Chem. 280:27205-27212(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Blue native/SDS-PAGE analysis reveals reduced expression of the mClCA3 protein in cystic fibrosis knock-out mice."
      Brouillard F., Bensalem N., Hinzpeter A., Tondelier D., Trudel S., Gruber A.D., Ollero M., Edelman A.
      Mol. Cell. Proteomics 4:1762-1775(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "Gob-5 contributes to goblet cell hyperplasia and modulates pulmonary tissue inflammation."
      Long A.J., Sypek J.P., Askew R., Fish S.C., Mason L.E., Williams C.M.M., Goldman S.J.
      Am. J. Respir. Cell Mol. Biol. 35:357-365(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    11. "Both cleavage products of the mCLCA3 protein are secreted soluble proteins."
      Mundhenk L., Alfalah M., Elble R.C., Pauli B.U., Naim H.Y., Gruber A.D.
      J. Biol. Chem. 281:30072-30080(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, PROCESSING.

    Entry informationi

    Entry nameiCLCA1_MOUSE
    AccessioniPrimary (citable) accession number: Q9D7Z6
    Secondary accession number(s): O88826, Q8R049
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3