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Q9D7Z6 (CLCA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-activated chloride channel regulator 1
Alternative name(s):
Calcium-activated chloride channel family member 3
Short name=mCLCA3
Protein gob-5
Gene names
Name:Clca1
Synonyms:Clca3, Gob5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length913 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. Ref.2 Ref.5 Ref.10

Subcellular location

Secretedextracellular space. Note: The 75 kDa N-terminal and a 35 kDa C-terminal products are fully secreted into extracellular environment as a soluble complex of two glycoproteins. Ref.6 Ref.8 Ref.11

Tissue specificity

Exclusively expressed in the digestive and respiratory tracts and in the uterus (at protein level). Expressed in small intestine, colon, stomach, and uterus and slightly expressed in trachea tissue. Exclusively expressed in the mucin granule membranes of gastrointestinal, respiratory, and uterine goblet cells and other mucin-producing cells. In the colon, expressed in the surface mucous cells. In the stomach highly expressed in the surface epithelium in the pylorus. Strongly expressed in the airway epithelium of lung tissues associated with airway hyperresponsiveness (AHR). Ref.1 Ref.5 Ref.6

Induction

By IL3, IL4 and IL9 in the lung. Increases in the bronchiolar epithelium of asbestos-induced fibrogenesis. Decreases in cystic fibrosis knockout mice. Ref.2 Ref.7 Ref.9

Post-translational modification

The 110 kDa translation product is cleaved in the endoplasmic reticulum into a 75 kDa N-terminal and a 35 kDa C-terminal products that remain physically associated with each other. Ref.6 Ref.11

Glycosylated. Ref.6 Ref.11

Disruption phenotype

Mice exhibit significantly increased bronchoalveolar lavage (BAL) inflammation consisted predominantly of neutrophils; have decreased goblet cell hyperplasia as well as decreased mucus production; have decreased airway hypersensitivity after cholinergic provocation with methacholine. Ref.10

Sequence similarities

Belongs to the CLCR family.

Contains 1 VWFA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 913892Calcium-activated chloride channel regulator 1
PRO_0000333692

Regions

Domain307 – 476170VWFA

Amino acid modifications

Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation7701N-linked (GlcNAc...) Potential
Glycosylation8041N-linked (GlcNAc...) Potential
Glycosylation8101N-linked (GlcNAc...) Potential
Glycosylation8361N-linked (GlcNAc...) Potential
Glycosylation8871N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4691L → F in AAH28343. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9D7Z6 [UniParc].

Last modified December 1, 2001. Version 2.
Checksum: A7FA2F9E1089806D

FASTA913100,071
        10         20         30         40         50         60 
MESLKSPVFL LILHLLEGVL SESLIQLNNN GYEGIVIAID HDVPEDEALI QHIKDMVTQA 

        70         80         90        100        110        120 
SPYLFEATGK RFYFKNVAIL IPESWKAKPE YTRPKLETFK NADVLVSTTS PLGNDEPYTE 

       130        140        150        160        170        180 
HIGACGEKGI RIHLTPDFLA GKKLTQYGPQ DRTFVHEWAH FRWGVFNEYN NDEKFYLSKG 

       190        200        210        220        230        240 
KPQAVRCSAA ITGKNQVRRC QGGSCITNGK CVIDRVTGLY KDNCVFVPDP HQNEKASIMF 

       250        260        270        280        290        300 
NQNINSVVEF CTEKNHNQEA PNDQNQRCNL RSTWEVIQES EDFKQTTPMT AQPPAPTFSL 

       310        320        330        340        350        360 
LQIGQRIVCL VLDKSGSMLN DDRLNRMNQA SRLFLLQTVE QGSWVGMVTF DSAAYVQSEL 

       370        380        390        400        410        420 
KQLNSGADRD LLIKHLPTVS AGGTSICSGL RTAFTVIKKK YPTDGSEIVL LTDGEDNTIS 

       430        440        450        460        470        480 
SCFDLVKQSG AIIHTVALGP AAAKELEQLS KMTGGLQTYS SDQVQNNGLV DAFAALSSGN 

       490        500        510        520        530        540 
AAIAQHSIQL ESRGVNLQNN QWMNGSVIVD SSVGKDTLFL ITWTTHPPTI FIWDPSGVEQ 

       550        560        570        580        590        600 
NGFILDTTTK VAYLQVPGTA KVGFWKYSIQ ASSQTLTLTV TSRAASATLP PITVTPVVNK 

       610        620        630        640        650        660 
NTGKFPSPVT VYASIRQGAS PILRASVTAL IESVNGKTVT LELLDNGAGA DATKNDGVYS 

       670        680        690        700        710        720 
RFFTAFDANG RYSVKIWALG GVTSDRQRAA PPKNRAMYID GWIEDGEVRM NPPRPETSYV 

       730        740        750        760        770        780 
QDKQLCFSRT SSGGSFVATN VPAAAPIPDL FPPCQITDLK ASIQGQNLVN LTWTAPGDDY 

       790        800        810        820        830        840 
DHGRASNYII RMSTSIVDLR DHFNTSLQVN TTGLIPKEAS SEEIFEFELG GNTFGNGTDI 

       850        860        870        880        890        900 
FIAIQAVDKS NLKSEISNIA RVSVFIPAQE PPIPEDSTPP CPDISINSTI PGIHVLKIMW 

       910 
KWLGEMQVTL GLH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and identification of the gene gob-5, which is expressed in intestinal goblet cells in mice."
Komiya T., Tanigawa Y., Hirohashi S.
Biochem. Biophys. Res. Commun. 255:347-351(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Intestine.
[2]"Characterization of a calcium-activated chloride channel as a shared target of Th2 cytokine pathways and its potential involvement in asthma."
Zhou Y., Dong Q., Louahed J., Dragwa C., Savio D., Huang M., Weiss C., Tomer Y., McLane M.P., Nicolaides N.C., Levitt R.C.
Am. J. Respir. Cell Mol. Biol. 25:486-491(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Colon and Stomach.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
[5]"Role of gob-5 in mucus overproduction and airway hyperresponsiveness in asthma."
Nakanishi A., Morita S., Iwashita H., Sagiya Y., Ashida Y., Shirafuji H., Fujisawa Y., Nishimura O., Fujino M.
Proc. Natl. Acad. Sci. U.S.A. 98:5175-5180(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"The murine mCLCA3 (alias gob-5) protein is located in the mucin granule membranes of intestinal, respiratory, and uterine goblet cells."
Leverkoehne I., Gruber A.D.
J. Histochem. Cytochem. 50:829-838(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PROTEOLYTIC PROCESSING, GLYCOSYLATION.
[7]"Gene expression profiles reveal increased mClca3 (Gob5) expression and mucin production in a murine model of asbestos-induced fibrogenesis."
Sabo-Attwood T., Ramos-Nino M., Bond J., Butnor K.J., Heintz N., Gruber A.D., Steele C., Taatjes D.J., Vacek P., Mossman B.T.
Am. J. Pathol. 167:1243-1256(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[8]"hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels."
Gibson A., Lewis A.P., Affleck K., Aitken A.J., Meldrum E., Thompson N.
J. Biol. Chem. 280:27205-27212(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Blue native/SDS-PAGE analysis reveals reduced expression of the mClCA3 protein in cystic fibrosis knock-out mice."
Brouillard F., Bensalem N., Hinzpeter A., Tondelier D., Trudel S., Gruber A.D., Ollero M., Edelman A.
Mol. Cell. Proteomics 4:1762-1775(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[10]"Gob-5 contributes to goblet cell hyperplasia and modulates pulmonary tissue inflammation."
Long A.J., Sypek J.P., Askew R., Fish S.C., Mason L.E., Williams C.M.M., Goldman S.J.
Am. J. Respir. Cell Mol. Biol. 35:357-365(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]"Both cleavage products of the mCLCA3 protein are secreted soluble proteins."
Mundhenk L., Alfalah M., Elble R.C., Pauli B.U., Naim H.Y., Gruber A.D.
J. Biol. Chem. 281:30072-30080(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, PROCESSING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017156 mRNA. Translation: BAA33743.1.
AK008659 mRNA. Translation: BAB25815.2.
AK136476 mRNA. Translation: BAE22995.1.
BC028343 mRNA. Translation: AAH28343.1.
BC116318 mRNA. Translation: AAI16319.1.
BC116319 mRNA. Translation: AAI16320.1.
CCDSCCDS17889.1.
PIRJG0168.
RefSeqNP_059502.1. NM_017474.2.
UniGeneMm.33483.

3D structure databases

ProteinModelPortalQ9D7Z6.
SMRQ9D7Z6. Positions 305-477.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM87.001.

PTM databases

PhosphoSiteQ9D7Z6.

Proteomic databases

MaxQBQ9D7Z6.
PaxDbQ9D7Z6.
PRIDEQ9D7Z6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029919; ENSMUSP00000029919; ENSMUSG00000028255.
GeneID23844.
KEGGmmu:23844.
UCSCuc008rqf.2. mouse.

Organism-specific databases

CTD23844.
MGIMGI:1346342. Clca3.

Phylogenomic databases

eggNOGNOG42884.
GeneTreeENSGT00390000004810.
HOGENOMHOG000015107.
HOVERGENHBG005560.
InParanoidQ9D7Z6.
KOK05027.
OMAEPYTEQM.
OrthoDBEOG78WKR1.
PhylomeDBQ9D7Z6.
TreeFamTF328396.

Gene expression databases

BgeeQ9D7Z6.
GenevestigatorQ9D7Z6.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR004727. CaCC_prot.
IPR013642. Cl_channel_Ca.
IPR015394. DUF1973.
IPR002035. VWF_A.
[Graphical view]
PfamPF08434. CLCA_N. 1 hit.
PF09315. DUF1973. 1 hit.
[Graphical view]
SMARTSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
TIGRFAMsTIGR00868. hCaCC. 1 hit.
PROSITEPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio303523.
PROQ9D7Z6.
SOURCESearch...

Entry information

Entry nameCLCA1_MOUSE
AccessionPrimary (citable) accession number: Q9D7Z6
Secondary accession number(s): O88826, Q8R049
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot