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Q9D7X3

- DUS3_MOUSE

UniProt

Q9D7X3 - DUS3_MOUSE

Protein

Dual specificity protein phosphatase 3

Gene

Dusp3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2 By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei124 – 1241Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase phosphatase activity Source: RefGenome
    2. phosphoprotein phosphatase activity Source: MGI
    3. protein tyrosine/serine/threonine phosphatase activity Source: MGI
    4. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. in utero embryonic development Source: Ensembl
    2. negative regulation of ERK1 and ERK2 cascade Source: RefGenome
    3. negative regulation of JNK cascade Source: RefGenome
    4. negative regulation of T cell activation Source: RefGenome
    5. negative regulation of T cell receptor signaling pathway Source: RefGenome
    6. positive regulation of mitotic cell cycle Source: RefGenome
    7. protein dephosphorylation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    ReactomeiREACT_210064. ERKs are inactivated.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 3 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    T-DSP11
    Vaccinia H1-related phosphatase
    Short name:
    VHR
    Gene namesi
    Name:Dusp3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1919599. Dusp3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: RefGenome
    2. immunological synapse Source: Ensembl
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 185185Dual specificity protein phosphatase 3PRO_0000094796Add
    BLAST

    Proteomic databases

    MaxQBiQ9D7X3.
    PaxDbiQ9D7X3.
    PRIDEiQ9D7X3.

    PTM databases

    PhosphoSiteiQ9D7X3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9D7X3.
    BgeeiQ9D7X3.
    GenevestigatoriQ9D7X3.

    Interactioni

    Subunit structurei

    Interacts with VRK3, which seems to activate it's phosphatase activity.1 Publication

    Protein-protein interaction databases

    BioGridi215327. 3 interactions.
    IntActiQ9D7X3. 1 interaction.
    MINTiMINT-4093702.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D7X3.
    SMRiQ9D7X3. Positions 7-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini99 – 16971Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2453.
    GeneTreeiENSGT00550000074474.
    HOGENOMiHOG000233767.
    HOVERGENiHBG001524.
    InParanoidiQ9D7X3.
    KOiK17614.
    TreeFamiTF105128.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR020417. Atypical_DUSP.
    IPR020405. Atypical_DUSP_famA.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    PRINTSiPR01908. ADSPHPHTASE.
    PR01909. ADSPHPHTASEA.
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9D7X3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVVPRVYVG NASVAQDITQ    50
    LQKLGITHVL NAAEGRSFMH VNTSASFYED SGITYLGIKA NDTQEFNLSA 100
    YFERATDFID QALAHKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA 150
    LSTVRQNREI GPNDGFLAQL CQLNDRLAKE GKVKL 185
    Length:185
    Mass (Da):20,472
    Last modified:June 1, 2001 - v1
    Checksum:i62E519E41BE575D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF280809 mRNA. Translation: AAK69507.1.
    AK008734 mRNA. Translation: BAB25864.1.
    BC016269 mRNA. Translation: AAH16269.1.
    CCDSiCCDS25482.1.
    RefSeqiNP_082483.1. NM_028207.3.
    UniGeneiMm.196295.
    Mm.491149.

    Genome annotation databases

    EnsembliENSMUST00000003612; ENSMUSP00000003612; ENSMUSG00000003518.
    ENSMUST00000107172; ENSMUSP00000102790; ENSMUSG00000003518.
    GeneIDi72349.
    KEGGimmu:72349.
    UCSCiuc007lqa.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF280809 mRNA. Translation: AAK69507.1 .
    AK008734 mRNA. Translation: BAB25864.1 .
    BC016269 mRNA. Translation: AAH16269.1 .
    CCDSi CCDS25482.1.
    RefSeqi NP_082483.1. NM_028207.3.
    UniGenei Mm.196295.
    Mm.491149.

    3D structure databases

    ProteinModelPortali Q9D7X3.
    SMRi Q9D7X3. Positions 7-184.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215327. 3 interactions.
    IntActi Q9D7X3. 1 interaction.
    MINTi MINT-4093702.

    PTM databases

    PhosphoSitei Q9D7X3.

    Proteomic databases

    MaxQBi Q9D7X3.
    PaxDbi Q9D7X3.
    PRIDEi Q9D7X3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003612 ; ENSMUSP00000003612 ; ENSMUSG00000003518 .
    ENSMUST00000107172 ; ENSMUSP00000102790 ; ENSMUSG00000003518 .
    GeneIDi 72349.
    KEGGi mmu:72349.
    UCSCi uc007lqa.2. mouse.

    Organism-specific databases

    CTDi 1845.
    MGIi MGI:1919599. Dusp3.

    Phylogenomic databases

    eggNOGi COG2453.
    GeneTreei ENSGT00550000074474.
    HOGENOMi HOG000233767.
    HOVERGENi HBG001524.
    InParanoidi Q9D7X3.
    KOi K17614.
    TreeFami TF105128.

    Enzyme and pathway databases

    Reactomei REACT_210064. ERKs are inactivated.

    Miscellaneous databases

    NextBioi 336089.
    PROi Q9D7X3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D7X3.
    Bgeei Q9D7X3.
    Genevestigatori Q9D7X3.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR020417. Atypical_DUSP.
    IPR020405. Atypical_DUSP_famA.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    [Graphical view ]
    PRINTSi PR01908. ADSPHPHTASE.
    PR01909. ADSPHPHTASEA.
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of novel putative dual specificity protein phosphatases T-DSP6 and T-DSP11 with low molecular masses containing a single catalytic domain but not cdc25 homology domain."
      Aoyama K., Matsuda T., Aoki N.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Stomach.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 90-104 AND 159-176, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.
    5. "Negative regulation of ERK activity by VRK3-mediated activation of VHR phosphatase."
      Kang T.H., Kim K.T.
      Nat. Cell Biol. 8:863-869(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VRK3.

    Entry informationi

    Entry nameiDUS3_MOUSE
    AccessioniPrimary (citable) accession number: Q9D7X3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3