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Protein

Mediator of RNA polymerase II transcription subunit 8

Gene

Med8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 8
Alternative name(s):
Activator-recruited cofactor 32 kDa component
Short name:
ARC32
Mediator complex subunit 8
Gene namesi
Name:Med8
ORF Names:MNCb-2386
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915269. Med8.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Mediator of RNA polymerase II transcription subunit 8PRO_0000096395Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei82 – 821PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D7W5.
MaxQBiQ9D7W5.
PaxDbiQ9D7W5.
PeptideAtlasiQ9D7W5.
PRIDEiQ9D7W5.

PTM databases

iPTMnetiQ9D7W5.
PhosphoSiteiQ9D7W5.

Expressioni

Gene expression databases

BgeeiQ9D7W5.
ExpressionAtlasiQ9D7W5. baseline and differential.
GenevisibleiQ9D7W5. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the Elongin BC complex (TCEB1 and TCEB2), CUL2 and RBX1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MED17Q9NVC62EBI-7990252,EBI-394562From a different organism.
Med20Q9R0X02EBI-7990252,EBI-398698
MED22Q155282EBI-7990252,EBI-394687From a different organism.
MED27Q6P2C82EBI-7990252,EBI-394603From a different organism.
MED28Q9H2042EBI-7990252,EBI-514199From a different organism.
MED29Q9NX702EBI-7990252,EBI-394656From a different organism.
Med30Q9CQI92EBI-7990252,EBI-309220
MED6O755862EBI-7990252,EBI-394624From a different organism.

Protein-protein interaction databases

BioGridi219788. 1 interaction.
IntActiQ9D7W5. 13 interactions.
MINTiMINT-4101718.
STRINGi10090.ENSMUSP00000019229.

Structurei

3D structure databases

ProteinModelPortaliQ9D7W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni142 – 15110Interaction with the Elongin BC complexBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 2929Sequence analysisAdd
BLAST
Coiled coili133 – 16331Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3583. Eukaryota.
ENOG410XNQY. LUCA.
GeneTreeiENSGT00390000011838.
HOGENOMiHOG000231149.
HOVERGENiHBG009716.
InParanoidiQ9D7W5.
KOiK15129.
OMAiDVAQKQI.
PhylomeDBiQ9D7W5.
TreeFamiTF316778.

Family and domain databases

InterProiIPR019364. Mediatior_Med8_fun/met.
[Graphical view]
PfamiPF10232. Med8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D7W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQREEKQLEA SLDALLNQVA DLKNSLGSFI YKLENEYDRL TWPSVLDSFA
60 70 80 90 100
LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDLM RQTEGRVPVF
110 120 130 140 150
SHEVVPDHLR TKPDPEVEEQ EKQLTTDAAR IGADAAQKQI QSLNKMCSNL
160 170 180 190 200
LEKISKEERE SESGGLRPNK QTFNPGDTNA LVAAVAFGKG LSNWRPSGSS
210 220 230 240 250
GPGQPGQPGA GTILAGASGL PQVQMPGAPN QQQPMLSGVQ MAQAGQPGKM
260
PSGIKTNIKS ASMHPYQR
Length:268
Mass (Da):29,199
Last modified:June 1, 2001 - v1
Checksum:i20A0C02E00BCA667
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571N → NSFALLSGQLN in BAA95113 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041805 mRNA. Translation: BAA95113.1.
AK008759 mRNA. Translation: BAB25879.1.
AK147032 mRNA. Translation: BAE27624.1.
AK148025 mRNA. Translation: BAE28295.1.
BC002315 mRNA. Translation: AAH02315.1.
BC021870 mRNA. Translation: AAH21870.1.
CCDSiCCDS38854.1.
RefSeqiNP_001277617.1. NM_001290688.1.
NP_064384.2. NM_020000.3.
NP_776067.1. NM_173719.3.
UniGeneiMm.259567.

Genome annotation databases

EnsembliENSMUST00000019229; ENSMUSP00000019229; ENSMUSG00000006392.
GeneIDi80509.
KEGGimmu:80509.
UCSCiuc008ujw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041805 mRNA. Translation: BAA95113.1.
AK008759 mRNA. Translation: BAB25879.1.
AK147032 mRNA. Translation: BAE27624.1.
AK148025 mRNA. Translation: BAE28295.1.
BC002315 mRNA. Translation: AAH02315.1.
BC021870 mRNA. Translation: AAH21870.1.
CCDSiCCDS38854.1.
RefSeqiNP_001277617.1. NM_001290688.1.
NP_064384.2. NM_020000.3.
NP_776067.1. NM_173719.3.
UniGeneiMm.259567.

3D structure databases

ProteinModelPortaliQ9D7W5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219788. 1 interaction.
IntActiQ9D7W5. 13 interactions.
MINTiMINT-4101718.
STRINGi10090.ENSMUSP00000019229.

PTM databases

iPTMnetiQ9D7W5.
PhosphoSiteiQ9D7W5.

Proteomic databases

EPDiQ9D7W5.
MaxQBiQ9D7W5.
PaxDbiQ9D7W5.
PeptideAtlasiQ9D7W5.
PRIDEiQ9D7W5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019229; ENSMUSP00000019229; ENSMUSG00000006392.
GeneIDi80509.
KEGGimmu:80509.
UCSCiuc008ujw.2. mouse.

Organism-specific databases

CTDi112950.
MGIiMGI:1915269. Med8.

Phylogenomic databases

eggNOGiKOG3583. Eukaryota.
ENOG410XNQY. LUCA.
GeneTreeiENSGT00390000011838.
HOGENOMiHOG000231149.
HOVERGENiHBG009716.
InParanoidiQ9D7W5.
KOiK15129.
OMAiDVAQKQI.
PhylomeDBiQ9D7W5.
TreeFamiTF316778.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

PROiQ9D7W5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D7W5.
ExpressionAtlasiQ9D7W5. baseline and differential.
GenevisibleiQ9D7W5. MM.

Family and domain databases

InterProiIPR019364. Mediatior_Med8_fun/met.
[Graphical view]
PfamiPF10232. Med8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart and Stomach.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiMED8_MOUSE
AccessioniPrimary (citable) accession number: Q9D7W5
Secondary accession number(s): Q3UGB4, Q99LM4, Q9JJ84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.