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Protein

Mediator of RNA polymerase II transcription subunit 8

Gene

Med8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 8
Alternative name(s):
Activator-recruited cofactor 32 kDa component
Short name:
ARC32
Mediator complex subunit 8
Gene namesi
Name:Med8
ORF Names:MNCb-2386
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915269 Med8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963951 – 268Mediator of RNA polymerase II transcription subunit 8Add BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D7W5
MaxQBiQ9D7W5
PaxDbiQ9D7W5
PeptideAtlasiQ9D7W5
PRIDEiQ9D7W5

PTM databases

iPTMnetiQ9D7W5
PhosphoSitePlusiQ9D7W5

Expressioni

Gene expression databases

BgeeiENSMUSG00000006392
ExpressionAtlasiQ9D7W5 baseline and differential
GenevisibleiQ9D7W5 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the Elongin BC complex (ELOB and ELOC), CUL2 and RBX1 (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi219788, 1 interactor
CORUMiQ9D7W5
IntActiQ9D7W5, 13 interactors
MINTiQ9D7W5
STRINGi10090.ENSMUSP00000019229

Structurei

3D structure databases

ProteinModelPortaliQ9D7W5
SMRiQ9D7W5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni142 – 151Interaction with the Elongin BC complexBy similarity10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 29Sequence analysisAdd BLAST29
Coiled coili133 – 163Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the Mediator complex subunit 8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3583 Eukaryota
ENOG410XNQY LUCA
GeneTreeiENSGT00390000011838
HOGENOMiHOG000231149
HOVERGENiHBG009716
InParanoidiQ9D7W5
KOiK15129
OMAiVAQKQIQ
OrthoDBiEOG091G0VMU
PhylomeDBiQ9D7W5
TreeFamiTF316778

Family and domain databases

InterProiView protein in InterPro
IPR019364 Mediatior_Med8_fun/met
PANTHERiPTHR13074 PTHR13074, 1 hit
PfamiView protein in Pfam
PF10232 Med8, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D7W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQREEKQLEA SLDALLNQVA DLKNSLGSFI YKLENEYDRL TWPSVLDSFA
60 70 80 90 100
LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDLM RQTEGRVPVF
110 120 130 140 150
SHEVVPDHLR TKPDPEVEEQ EKQLTTDAAR IGADAAQKQI QSLNKMCSNL
160 170 180 190 200
LEKISKEERE SESGGLRPNK QTFNPGDTNA LVAAVAFGKG LSNWRPSGSS
210 220 230 240 250
GPGQPGQPGA GTILAGASGL PQVQMPGAPN QQQPMLSGVQ MAQAGQPGKM
260
PSGIKTNIKS ASMHPYQR
Length:268
Mass (Da):29,199
Last modified:June 1, 2001 - v1
Checksum:i20A0C02E00BCA667
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57N → NSFALLSGQLN in BAA95113 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041805 mRNA Translation: BAA95113.1
AK008759 mRNA Translation: BAB25879.1
AK147032 mRNA Translation: BAE27624.1
AK148025 mRNA Translation: BAE28295.1
BC002315 mRNA Translation: AAH02315.1
BC021870 mRNA Translation: AAH21870.1
CCDSiCCDS38854.1
RefSeqiNP_001277617.1, NM_001290688.1
NP_064384.2, NM_020000.3
NP_776067.1, NM_173719.3
UniGeneiMm.259567

Genome annotation databases

EnsembliENSMUST00000019229; ENSMUSP00000019229; ENSMUSG00000006392
GeneIDi80509
KEGGimmu:80509
UCSCiuc008ujw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMED8_MOUSE
AccessioniPrimary (citable) accession number: Q9D7W5
Secondary accession number(s): Q3UGB4, Q99LM4, Q9JJ84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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