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Protein

Chitotriosidase-1

Gene

Chit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens (By similarity).By similarity1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140Proton donorPROSITE-ProRule annotation1
Binding sitei269ChitooligosaccharideBy similarity1
Binding sitei356ChitooligosaccharideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-71387. Metabolism of carbohydrates.

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitotriosidase-1 (EC:3.2.1.14)
Alternative name(s):
Chitinase-1
Gene namesi
Name:Chit1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1919134. Chit1.

Subcellular locationi

  • Secreted 1 Publication
  • Lysosome By similarity

  • Note: A small proportion is lysosomal.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136D → N: Loss of activity; when associated with Q-140. 1 Publication1
Mutagenesisi140E → Q: Loss of activity; when associated with N-136. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000001194222 – 464Chitotriosidase-1Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 51PROSITE-ProRule annotation
Disulfide bondi307 ↔ 368PROSITE-ProRule annotation
Disulfide bondi448 ↔ 461PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9D7Q1.
PeptideAtlasiQ9D7Q1.
PRIDEiQ9D7Q1.

PTM databases

PhosphoSitePlusiQ9D7Q1.

Expressioni

Tissue specificityi

Highly expressed in tongue, stomach, kidney, brain, skin, testis, and bone marrow. Low level of expression was found in lung, heart, spleen, small intestine, and liver. Not detectable in pancreas, salivary gland, large intestine, uterus, or peripheral blood mononuclear cells (PBMC).2 Publications

Gene expression databases

BgeeiENSMUSG00000026450.
CleanExiMM_CHIT1.
ExpressionAtlasiQ9D7Q1. baseline and differential.
GenevisibleiQ9D7Q1. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083666.

Structurei

3D structure databases

ProteinModelPortaliQ9D7Q1.
SMRiQ9D7Q1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini415 – 464Chitin-binding type-2PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 71Chitooligosaccharide bindingBy similarity2
Regioni97 – 100Chitooligosaccharide bindingBy similarity4
Regioni210 – 213Chitooligosaccharide bindingBy similarity4

Sequence similaritiesi

Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ9D7Q1.
KOiK01183.
OMAiYEENDHE.
OrthoDBiEOG091G014W.
PhylomeDBiQ9D7Q1.
TreeFamiTF315610.

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D7Q1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQSLAWAGV MTLLMVQWGS AAKLVCYLTN WSQYRTEAVR FFPRDVDPNL
60 70 80 90 100
CTHVIFAFAG MDNHQLSTVE HNDELLYQEL NSLKTKNPKL KTLLAVGGWT
110 120 130 140 150
FGTQKFTDMV ATASNRQTFV KSALSFLRTQ GFDGLDLDWE FPGGRGSPTV
160 170 180 190 200
DKERFTALIQ DLAKAFQEEA QSSGKERLLL TAAVPSDRGL VDAGYEVDKI
210 220 230 240 250
AQSLDFINLM AYDFHSSLEK TTGHNSPLYK RQGESGAAAE QNVDAAVTLW
260 270 280 290 300
LQKGTPASKL ILGMPTYGRS FTLASSSDNG VGAPATGPGA PGPYTKDKGV
310 320 330 340 350
LAYYEACSWK ERHRIEDQKV PYAFQDNQWV SFDDVESFKA KAAYLKQKGL
360 370 380 390 400
GGAMVWVLDL DDFKGSFCNQ GPYPLIRTLR QELNLPSETP RSPEQIIPEP
410 420 430 440 450
RPSSMPEQGP SPGLDNFCQG KADGVYPNPG DESTYYNCGG GRLFQQSCPP
460
GLVFRASCKC CTWS
Length:464
Mass (Da):51,112
Last modified:September 5, 2006 - v2
Checksum:i6276B6A414B4E96A
GO
Isoform 2 (identifier: Q9D7Q1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     343-396: AYLKQKGLGG...SETPRSPEQI → KLMGSTPTLE...KFLEPHPTPV
     397-464: Missing.

Show »
Length:396
Mass (Da):43,413
Checksum:i32BB674B61AF7123
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020143343 – 396AYLKQ…SPEQI → KLMGSTPTLETSPLTTTVEE GGCSSRAVLQAWCLEPLANV VPGAKFLEPHPTPV in isoform 2. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_020144397 – 464Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458654 mRNA. Translation: AAR14312.1.
AY536287 mRNA. Translation: AAS47832.1.
AK009012 mRNA. Translation: BAB26025.1.
CCDSiCCDS15304.1. [Q9D7Q1-1]
CCDS69943.1. [Q9D7Q1-2]
RefSeqiNP_001271453.1. NM_001284524.1. [Q9D7Q1-2]
NP_001271454.1. NM_001284525.1. [Q9D7Q1-1]
NP_082255.1. NM_027979.2. [Q9D7Q1-1]
XP_006529942.1. XM_006529879.2. [Q9D7Q1-1]
XP_006529943.1. XM_006529880.1. [Q9D7Q1-1]
XP_006529944.1. XM_006529881.2. [Q9D7Q1-2]
UniGeneiMm.328268.

Genome annotation databases

EnsembliENSMUST00000086475; ENSMUSP00000083666; ENSMUSG00000026450. [Q9D7Q1-1]
ENSMUST00000159963; ENSMUSP00000123979; ENSMUSG00000026450. [Q9D7Q1-1]
ENSMUST00000160060; ENSMUSP00000124331; ENSMUSG00000026450. [Q9D7Q1-2]
GeneIDi71884.
KEGGimmu:71884.
UCSCiuc007crf.2. mouse. [Q9D7Q1-1]
uc033flh.1. mouse. [Q9D7Q1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458654 mRNA. Translation: AAR14312.1.
AY536287 mRNA. Translation: AAS47832.1.
AK009012 mRNA. Translation: BAB26025.1.
CCDSiCCDS15304.1. [Q9D7Q1-1]
CCDS69943.1. [Q9D7Q1-2]
RefSeqiNP_001271453.1. NM_001284524.1. [Q9D7Q1-2]
NP_001271454.1. NM_001284525.1. [Q9D7Q1-1]
NP_082255.1. NM_027979.2. [Q9D7Q1-1]
XP_006529942.1. XM_006529879.2. [Q9D7Q1-1]
XP_006529943.1. XM_006529880.1. [Q9D7Q1-1]
XP_006529944.1. XM_006529881.2. [Q9D7Q1-2]
UniGeneiMm.328268.

3D structure databases

ProteinModelPortaliQ9D7Q1.
SMRiQ9D7Q1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083666.

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

PTM databases

PhosphoSitePlusiQ9D7Q1.

Proteomic databases

PaxDbiQ9D7Q1.
PeptideAtlasiQ9D7Q1.
PRIDEiQ9D7Q1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086475; ENSMUSP00000083666; ENSMUSG00000026450. [Q9D7Q1-1]
ENSMUST00000159963; ENSMUSP00000123979; ENSMUSG00000026450. [Q9D7Q1-1]
ENSMUST00000160060; ENSMUSP00000124331; ENSMUSG00000026450. [Q9D7Q1-2]
GeneIDi71884.
KEGGimmu:71884.
UCSCiuc007crf.2. mouse. [Q9D7Q1-1]
uc033flh.1. mouse. [Q9D7Q1-2]

Organism-specific databases

CTDi1118.
MGIiMGI:1919134. Chit1.

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ9D7Q1.
KOiK01183.
OMAiYEENDHE.
OrthoDBiEOG091G014W.
PhylomeDBiQ9D7Q1.
TreeFamiTF315610.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-71387. Metabolism of carbohydrates.

Miscellaneous databases

PROiQ9D7Q1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026450.
CleanExiMM_CHIT1.
ExpressionAtlasiQ9D7Q1. baseline and differential.
GenevisibleiQ9D7Q1. MM.

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIT1_MOUSE
AccessioniPrimary (citable) accession number: Q9D7Q1
Secondary accession number(s): Q6QJD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.