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Q9D7Q1

- CHIT1_MOUSE

UniProt

Q9D7Q1 - CHIT1_MOUSE

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Protein

Chitotriosidase-1

Gene

Chit1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens (By similarity).By similarity

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei140 – 1401Proton donorPROSITE-ProRule annotation
Binding sitei269 – 2691ChitooligosaccharideBy similarity
Binding sitei356 – 3561ChitooligosaccharideBy similarity

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
  2. endochitinase activity Source: Ensembl

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitotriosidase-1 (EC:3.2.1.14)
Alternative name(s):
Chitinase-1
Gene namesi
Name:Chit1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1919134. Chit1.

Subcellular locationi

Secreted 1 Publication. Lysosome By similarity
Note: A small proportion is lysosomal.By similarity

GO - Cellular componenti

  1. extracellular space Source: Ensembl
  2. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi136 – 1361D → N: Loss of activity; when associated with Q-140. 1 Publication
Mutagenesisi140 – 1401E → Q: Loss of activity; when associated with N-136. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 464443Chitotriosidase-1PRO_0000011942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 51PROSITE-ProRule annotation
Disulfide bondi307 ↔ 368PROSITE-ProRule annotation
Disulfide bondi448 ↔ 461PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9D7Q1.
PaxDbiQ9D7Q1.
PRIDEiQ9D7Q1.

Expressioni

Tissue specificityi

Highly expressed in tongue, stomach, kidney, brain, skin, testis, and bone marrow. Low level of expression was found in lung, heart, spleen, small intestine, and liver. Not detectable in pancreas, salivary gland, large intestine, uterus, or peripheral blood mononuclear cells (PBMC).2 Publications

Gene expression databases

BgeeiQ9D7Q1.
CleanExiMM_CHIT1.
ExpressionAtlasiQ9D7Q1. baseline and differential.
GenevestigatoriQ9D7Q1.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083666.

Structurei

3D structure databases

ProteinModelPortaliQ9D7Q1.
SMRiQ9D7Q1. Positions 22-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini415 – 46450Chitin-binding type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 712Chitooligosaccharide bindingBy similarity
Regioni97 – 1004Chitooligosaccharide bindingBy similarity
Regioni210 – 2134Chitooligosaccharide bindingBy similarity

Sequence similaritiesi

Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3325.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ9D7Q1.
KOiK01183.
OMAiHRIKDQK.
OrthoDBiEOG7ZGX3G.
PhylomeDBiQ9D7Q1.
TreeFamiTF315610.

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9D7Q1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQSLAWAGV MTLLMVQWGS AAKLVCYLTN WSQYRTEAVR FFPRDVDPNL
60 70 80 90 100
CTHVIFAFAG MDNHQLSTVE HNDELLYQEL NSLKTKNPKL KTLLAVGGWT
110 120 130 140 150
FGTQKFTDMV ATASNRQTFV KSALSFLRTQ GFDGLDLDWE FPGGRGSPTV
160 170 180 190 200
DKERFTALIQ DLAKAFQEEA QSSGKERLLL TAAVPSDRGL VDAGYEVDKI
210 220 230 240 250
AQSLDFINLM AYDFHSSLEK TTGHNSPLYK RQGESGAAAE QNVDAAVTLW
260 270 280 290 300
LQKGTPASKL ILGMPTYGRS FTLASSSDNG VGAPATGPGA PGPYTKDKGV
310 320 330 340 350
LAYYEACSWK ERHRIEDQKV PYAFQDNQWV SFDDVESFKA KAAYLKQKGL
360 370 380 390 400
GGAMVWVLDL DDFKGSFCNQ GPYPLIRTLR QELNLPSETP RSPEQIIPEP
410 420 430 440 450
RPSSMPEQGP SPGLDNFCQG KADGVYPNPG DESTYYNCGG GRLFQQSCPP
460
GLVFRASCKC CTWS
Length:464
Mass (Da):51,112
Last modified:September 5, 2006 - v2
Checksum:i6276B6A414B4E96A
GO
Isoform 2 (identifier: Q9D7Q1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     343-396: AYLKQKGLGG...SETPRSPEQI → KLMGSTPTLE...KFLEPHPTPV
     397-464: Missing.

Show »
Length:396
Mass (Da):43,413
Checksum:i32BB674B61AF7123
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei343 – 39654AYLKQ…SPEQI → KLMGSTPTLETSPLTTTVEE GGCSSRAVLQAWCLEPLANV VPGAKFLEPHPTPV in isoform 2. 1 PublicationVSP_020143Add
BLAST
Alternative sequencei397 – 46468Missing in isoform 2. 1 PublicationVSP_020144Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458654 mRNA. Translation: AAR14312.1.
AY536287 mRNA. Translation: AAS47832.1.
AK009012 mRNA. Translation: BAB26025.1.
CCDSiCCDS15304.1. [Q9D7Q1-1]
CCDS69943.1. [Q9D7Q1-2]
RefSeqiNP_001271453.1. NM_001284524.1. [Q9D7Q1-2]
NP_001271454.1. NM_001284525.1. [Q9D7Q1-1]
NP_082255.1. NM_027979.2. [Q9D7Q1-1]
XP_006529942.1. XM_006529879.1. [Q9D7Q1-1]
XP_006529943.1. XM_006529880.1. [Q9D7Q1-1]
XP_006529944.1. XM_006529881.1. [Q9D7Q1-2]
UniGeneiMm.328268.

Genome annotation databases

EnsembliENSMUST00000086475; ENSMUSP00000083666; ENSMUSG00000026450. [Q9D7Q1-1]
ENSMUST00000159963; ENSMUSP00000123979; ENSMUSG00000026450. [Q9D7Q1-1]
ENSMUST00000160060; ENSMUSP00000124331; ENSMUSG00000026450. [Q9D7Q1-2]
GeneIDi71884.
KEGGimmu:71884.
UCSCiuc007crf.1. mouse. [Q9D7Q1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458654 mRNA. Translation: AAR14312.1 .
AY536287 mRNA. Translation: AAS47832.1 .
AK009012 mRNA. Translation: BAB26025.1 .
CCDSi CCDS15304.1. [Q9D7Q1-1 ]
CCDS69943.1. [Q9D7Q1-2 ]
RefSeqi NP_001271453.1. NM_001284524.1. [Q9D7Q1-2 ]
NP_001271454.1. NM_001284525.1. [Q9D7Q1-1 ]
NP_082255.1. NM_027979.2. [Q9D7Q1-1 ]
XP_006529942.1. XM_006529879.1. [Q9D7Q1-1 ]
XP_006529943.1. XM_006529880.1. [Q9D7Q1-1 ]
XP_006529944.1. XM_006529881.1. [Q9D7Q1-2 ]
UniGenei Mm.328268.

3D structure databases

ProteinModelPortali Q9D7Q1.
SMRi Q9D7Q1. Positions 22-383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000083666.

Protein family/group databases

CAZyi CBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Proteomic databases

MaxQBi Q9D7Q1.
PaxDbi Q9D7Q1.
PRIDEi Q9D7Q1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000086475 ; ENSMUSP00000083666 ; ENSMUSG00000026450 . [Q9D7Q1-1 ]
ENSMUST00000159963 ; ENSMUSP00000123979 ; ENSMUSG00000026450 . [Q9D7Q1-1 ]
ENSMUST00000160060 ; ENSMUSP00000124331 ; ENSMUSG00000026450 . [Q9D7Q1-2 ]
GeneIDi 71884.
KEGGi mmu:71884.
UCSCi uc007crf.1. mouse. [Q9D7Q1-1 ]

Organism-specific databases

CTDi 1118.
MGIi MGI:1919134. Chit1.

Phylogenomic databases

eggNOGi COG3325.
GeneTreei ENSGT00550000074323.
HOGENOMi HOG000111109.
HOVERGENi HBG011684.
InParanoidi Q9D7Q1.
KOi K01183.
OMAi HRIKDQK.
OrthoDBi EOG7ZGX3G.
PhylomeDBi Q9D7Q1.
TreeFami TF315610.

Miscellaneous databases

NextBioi 334840.
PROi Q9D7Q1.
SOURCEi Search...

Gene expression databases

Bgeei Q9D7Q1.
CleanExi MM_CHIT1.
ExpressionAtlasi Q9D7Q1. baseline and differential.
Genevestigatori Q9D7Q1.

Family and domain databases

Gene3Di 2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view ]
SMARTi SM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEi PS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional characterization of mouse chitotriosidase."
    Zheng T., Rabach M., Chen N.Y., Rabach L., Hu X., Elias J.A., Zhu Z.
    Gene 357:37-46(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-136 AND GLU-140.
    Strain: C57BL/6.
    Tissue: Tongue.
  2. "Marked differences in tissue-specific expression of chitinases in mouse and man."
    Boot R.G., Bussink A.P., Verhoek M., de Boer P.A.J., Moorman A.F., Aerts J.M.F.G.
    J. Histochem. Cytochem. 53:1283-1292(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Tongue.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Tongue.

Entry informationi

Entry nameiCHIT1_MOUSE
AccessioniPrimary (citable) accession number: Q9D7Q1
Secondary accession number(s): Q6QJD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3