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Protein

Iron-sulfur cluster assembly enzyme ISCU, mitochondrial

Gene

Iscu

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Alternative name(s):
NifU-like N-terminal domain-containing protein
NifU-like protein
Gene namesi
Name:Iscu
Synonyms:Nifun
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1913633 Iscu

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionSequence analysisAdd BLAST35
ChainiPRO_000001969336 – 168Iron-sulfur cluster assembly enzyme ISCU, mitochondrialAdd BLAST133

Proteomic databases

EPDiQ9D7P6
MaxQBiQ9D7P6
PaxDbiQ9D7P6
PeptideAtlasiQ9D7P6
PRIDEiQ9D7P6

PTM databases

iPTMnetiQ9D7P6
PhosphoSitePlusiQ9D7P6

Expressioni

Gene expression databases

BgeeiENSMUSG00000025825
ExpressionAtlasiQ9D7P6 baseline and differential
GenevisibleiQ9D7P6 MM

Interactioni

Subunit structurei

Binds NFS1. Interacts with HSCB. Interacts with GLRX5. Interacts with HSPA9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ9D7P6
IntActiQ9D7P6, 1 interactor
MINTiQ9D7P6
STRINGi10090.ENSMUSP00000026937

Structurei

Secondary structure

1168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi58 – 67Combined sources10
Turni68 – 71Combined sources4
Beta strandi72 – 80Combined sources9
Beta strandi82 – 92Combined sources11
Helixi97 – 110Combined sources14
Helixi115 – 120Combined sources6
Helixi123 – 130Combined sources8
Helixi134 – 136Combined sources3
Helixi137 – 159Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WFZNMR-A45-161[»]
ProteinModelPortaliQ9D7P6
SMRiQ9D7P6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D7P6

Family & Domainsi

Sequence similaritiesi

Belongs to the NifU family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3361 Eukaryota
COG0822 LUCA
GeneTreeiENSGT00390000015813
HOGENOMiHOG000069228
HOVERGENiHBG052621
InParanoidiQ9D7P6
KOiK22068
OMAiSMVTEMV
PhylomeDBiQ9D7P6
TreeFamiTF105422

Family and domain databases

CDDicd06664 IscU_like, 1 hit
InterProiView protein in InterPro
IPR011339 ISC_FeS_clus_asmbl_IscU
IPR002871 NIF_FeS_clus_asmbl_NifU_N
PANTHERiPTHR10093 PTHR10093, 1 hit
PfamiView protein in Pfam
PF01592 NifU_N, 1 hit
TIGRFAMsiTIGR01999 iscU, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D7P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATGAGRL RRAASALLLR SPRLPARELS APARLYHKKV VDHYENPRNV
60 70 80 90 100
GSLDKTSKNV GTGLVGAPAC GDVMKLQIQV DEKGKIVDAR FKTFGCGSAI
110 120 130 140 150
ASSSLATEWV KGKTVEEALT IKNTDIAKEL CLPPVKLHCS MLAEDAIKAA
160
LADYKLKQES KKEEPEKQ
Length:168
Mass (Da):18,098
Last modified:June 1, 2001 - v1
Checksum:i06B6350434FEAAF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009021 mRNA Translation: BAB26031.1
AK039834 mRNA Translation: BAC30464.1
AK083247 mRNA Translation: BAC38830.1
BC028800 mRNA Translation: AAH28800.1
BC048409 mRNA Translation: AAH48409.1
BC096049 mRNA Translation: AAH96049.1
CCDSiCCDS19553.1
RefSeqiNP_079802.1, NM_025526.4
UniGeneiMm.29497

Genome annotation databases

EnsembliENSMUST00000026937; ENSMUSP00000026937; ENSMUSG00000025825
GeneIDi66383
KEGGimmu:66383
UCSCiuc008yyp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiISCU_MOUSE
AccessioniPrimary (citable) accession number: Q9D7P6
Secondary accession number(s): Q4VBC7, Q8K344
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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