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Protein

Iron-sulfur cluster assembly enzyme ISCU, mitochondrial

Gene

Iscu

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Alternative name(s):
NifU-like N-terminal domain-containing protein
NifU-like protein
Gene namesi
Name:Iscu
Synonyms:Nifun
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1913633. Iscu.

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionSequence analysisAdd
BLAST
Chaini36 – 168133Iron-sulfur cluster assembly enzyme ISCU, mitochondrialPRO_0000019693Add
BLAST

Proteomic databases

EPDiQ9D7P6.
MaxQBiQ9D7P6.
PaxDbiQ9D7P6.
PRIDEiQ9D7P6.

PTM databases

PhosphoSiteiQ9D7P6.

Expressioni

Gene expression databases

BgeeiQ9D7P6.
ExpressionAtlasiQ9D7P6. baseline and differential.
GenevisibleiQ9D7P6. MM.

Interactioni

Subunit structurei

Binds NFS1 (By similarity). Interacts with HSCB (By similarity). Interacts with GLRX5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9D7P6. 1 interaction.
MINTiMINT-1848781.
STRINGi10090.ENSMUSP00000026937.

Structurei

Secondary structure

1
168
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi58 – 6710Combined sources
Turni68 – 714Combined sources
Beta strandi72 – 809Combined sources
Beta strandi82 – 9211Combined sources
Helixi97 – 11014Combined sources
Helixi115 – 1206Combined sources
Helixi123 – 1308Combined sources
Helixi134 – 1363Combined sources
Helixi137 – 15923Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WFZNMR-A45-161[»]
ProteinModelPortaliQ9D7P6.
SMRiQ9D7P6. Positions 45-161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D7P6.

Family & Domainsi

Sequence similaritiesi

Belongs to the NifU family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3361. Eukaryota.
COG0822. LUCA.
GeneTreeiENSGT00390000015813.
HOGENOMiHOG000069228.
HOVERGENiHBG052621.
InParanoidiQ9D7P6.
OMAiIGEAGNP.
OrthoDBiEOG7JMGGC.
PhylomeDBiQ9D7P6.
TreeFamiTF105422.

Family and domain databases

InterProiIPR011339. ISC_FeS_clus_asmbl_IscU.
IPR002871. NIF_FeS_clus_asmbl_NifU_N.
[Graphical view]
PfamiPF01592. NifU_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01999. iscU. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D7P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATGAGRL RRAASALLLR SPRLPARELS APARLYHKKV VDHYENPRNV
60 70 80 90 100
GSLDKTSKNV GTGLVGAPAC GDVMKLQIQV DEKGKIVDAR FKTFGCGSAI
110 120 130 140 150
ASSSLATEWV KGKTVEEALT IKNTDIAKEL CLPPVKLHCS MLAEDAIKAA
160
LADYKLKQES KKEEPEKQ
Length:168
Mass (Da):18,098
Last modified:June 1, 2001 - v1
Checksum:i06B6350434FEAAF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009021 mRNA. Translation: BAB26031.1.
AK039834 mRNA. Translation: BAC30464.1.
AK083247 mRNA. Translation: BAC38830.1.
BC028800 mRNA. Translation: AAH28800.1.
BC048409 mRNA. Translation: AAH48409.1.
BC096049 mRNA. Translation: AAH96049.1.
CCDSiCCDS19553.1.
RefSeqiNP_079802.1. NM_025526.4.
UniGeneiMm.29497.

Genome annotation databases

EnsembliENSMUST00000026937; ENSMUSP00000026937; ENSMUSG00000025825.
GeneIDi66383.
KEGGimmu:66383.
UCSCiuc008yyp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009021 mRNA. Translation: BAB26031.1.
AK039834 mRNA. Translation: BAC30464.1.
AK083247 mRNA. Translation: BAC38830.1.
BC028800 mRNA. Translation: AAH28800.1.
BC048409 mRNA. Translation: AAH48409.1.
BC096049 mRNA. Translation: AAH96049.1.
CCDSiCCDS19553.1.
RefSeqiNP_079802.1. NM_025526.4.
UniGeneiMm.29497.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WFZNMR-A45-161[»]
ProteinModelPortaliQ9D7P6.
SMRiQ9D7P6. Positions 45-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D7P6. 1 interaction.
MINTiMINT-1848781.
STRINGi10090.ENSMUSP00000026937.

PTM databases

PhosphoSiteiQ9D7P6.

Proteomic databases

EPDiQ9D7P6.
MaxQBiQ9D7P6.
PaxDbiQ9D7P6.
PRIDEiQ9D7P6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026937; ENSMUSP00000026937; ENSMUSG00000025825.
GeneIDi66383.
KEGGimmu:66383.
UCSCiuc008yyp.1. mouse.

Organism-specific databases

CTDi23479.
MGIiMGI:1913633. Iscu.

Phylogenomic databases

eggNOGiKOG3361. Eukaryota.
COG0822. LUCA.
GeneTreeiENSGT00390000015813.
HOGENOMiHOG000069228.
HOVERGENiHBG052621.
InParanoidiQ9D7P6.
OMAiIGEAGNP.
OrthoDBiEOG7JMGGC.
PhylomeDBiQ9D7P6.
TreeFamiTF105422.

Enzyme and pathway databases

ReactomeiR-MMU-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Miscellaneous databases

EvolutionaryTraceiQ9D7P6.
NextBioi321509.
PROiQ9D7P6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D7P6.
ExpressionAtlasiQ9D7P6. baseline and differential.
GenevisibleiQ9D7P6. MM.

Family and domain databases

InterProiIPR011339. ISC_FeS_clus_asmbl_IscU.
IPR002871. NIF_FeS_clus_asmbl_NifU_N.
[Graphical view]
PfamiPF01592. NifU_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01999. iscU. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hippocampus, Thymus and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland and Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.
  4. "Solution structure of iron-sulfur cluster protein (ISCU)."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 45-161.

Entry informationi

Entry nameiISCU_MOUSE
AccessioniPrimary (citable) accession number: Q9D7P6
Secondary accession number(s): Q4VBC7, Q8K344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.