Reviewed, UniProtKB/Swiss-Prot Q9D7H3 (RTC1_MOUSE)

Names and origin

Protein names

Recommended name:
    RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4
Alternative name(s):
    RNA terminal phosphate cyclase domain-containing protein 1

Gene names

Name:	Rtcd1
Synonyms:	Rpc1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.
Subunit structure	Monomer By similarity.
Subcellular location	Nucleus › nucleoplasm By similarity.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	RNA 3'-terminal phosphate cyclase		PRO_0000156411
Sites
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9D7H3-1 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 6829D420AB90DED3	FASTA	366	39,226
	10 20 30 40 50 60 MEGQRVEVDG GIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLRP QHLSGLEMVR 70 80 90 100 110 120 DLCGGHLEGA EIGSTEITFT PEKIRGGVHT ADTKTAGSVC LLMQVSMPCV LFAASPSELR 130 140 150 160 170 180 LKGGTNAEMA PQIDYTMMVF KPIAEKFGFT FNCDIKTRGY YPKGGGEVIV RVSPVKRLDP 190 200 210 220 230 240 INLTDRGSVT KIYGRAFVAG VLPLKVAKDM AAAAVRCIRK EIRDLYVSIQ PVQEARDQAF 250 260 270 280 290 300 GNGSGIIIVA ETSTGCLFAG SSLGKRGVNA DKAGIEAAEM LLANLRHGGT VDEYLQDQLI 310 320 330 340 350 360 IFMALANGIS RIKTGSVTLH TQTAIHFAEQ LAKAKFTVKK SEEEEDATKD TYVIECEGIG MANPHL

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Tongue.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK009245 mRNA. Translation: BAB26164.1.
IPI	IPI00110315.
UniGene	Mm.251228
3D structure databases
SMR	Q9D7H3. Positions 6-340.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9D7H3.
2-D gel databases
REPRODUCTION-2DPAGE	Q9D7H3.
Proteomic databases
PRIDE	Q9D7H3.
Genome annotation databases
Ensembl	ENSMUST00000000348; ENSMUSP00000000348; ENSMUSG00000000339; Mus musculus. [Genome view]
UCSC	uc008rcd.1. mouse.
Organism-specific databases
MGI	MGI:1913618. Rtcd1.
Phylogenomic databases
HOGENOM	HBG683539.
HOVERGEN	Q9D7H3.
InParanoid	Q9D7H3.
PhylomeDB	Q9D7H3.
Enzyme and pathway databases
BRENDA	6.5.1.4. 244.
Gene expression databases
ArrayExpress	Q9D7H3.
Bgee	Q9D7H3.
CleanEx	MM_RTCD1.
Genevestigator	Q9D7H3.
GermOnline	ENSMUSG00000000339. Mus musculus.
Family and domain databases
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR016443. RNA3'_term_phos_cycl-like_euk. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
PIRSF	PIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	321465.
PMAP-CutDB	Q9D7H3.
SOURCE	Search...

Entry information

Entry name

RTC1_MOUSE

Accession

Primary (citable) accession number: Q9D7H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)