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Q9D7G0

- PRPS1_MOUSE

UniProt

Q9D7G0 - PRPS1_MOUSE

Protein

Ribose-phosphate pyrophosphokinase 1

Gene

Prps1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 4 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

    Catalytic activityi

    ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by magnesium and inorganic phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi128 – 1281MagnesiumSequence Analysis
    Metal bindingi130 – 1301MagnesiumSequence Analysis
    Binding sitei130 – 1301ATPBy similarity
    Metal bindingi139 – 1391MagnesiumSequence Analysis
    Metal bindingi143 – 1431MagnesiumSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi96 – 1016ATPBy similarity

    GO - Molecular functioni

    1. ADP binding Source: Ensembl
    2. AMP binding Source: Ensembl
    3. ATP binding Source: UniProtKB
    4. carbohydrate binding Source: Ensembl
    5. GDP binding Source: Ensembl
    6. kinase activity Source: UniProtKB-KW
    7. magnesium ion binding Source: Ensembl
    8. protein homodimerization activity Source: UniProtKB
    9. ribose phosphate diphosphokinase activity Source: UniProtKB

    GO - Biological processi

    1. 5-phosphoribose 1-diphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. AMP biosynthetic process Source: Ensembl
    3. organ regeneration Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00087; UER00172.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribose-phosphate pyrophosphokinase 1 (EC:2.7.6.1)
    Alternative name(s):
    Phosphoribosyl pyrophosphate synthase I
    Short name:
    PRS-I
    Gene namesi
    Name:Prps1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:97775. Prps1.

    Subcellular locationi

    GO - Cellular componenti

    1. ribose phosphate diphosphokinase complex Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 318317Ribose-phosphate pyrophosphokinase 1PRO_0000141072Add
    BLAST

    Proteomic databases

    MaxQBiQ9D7G0.
    PaxDbiQ9D7G0.
    PRIDEiQ9D7G0.

    2D gel databases

    REPRODUCTION-2DPAGEQ9D7G0.

    PTM databases

    PhosphoSiteiQ9D7G0.

    Expressioni

    Developmental stagei

    Expressed in both vestibular and cochlea hair cells in early developing and postnatal mice and can also be detected in the spiral ganglion cells in at post natal day 6.1 Publication

    Gene expression databases

    ArrayExpressiQ9D7G0.
    BgeeiQ9D7G0.
    CleanExiMM_PRPS1.
    GenevestigatoriQ9D7G0.

    Interactioni

    Subunit structurei

    Homodimer. The active form is probably a hexamer composed of 3 homodimers By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202401. 1 interaction.
    IntActiQ9D7G0. 2 interactions.
    MINTiMINT-1858366.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D7G0.
    SMRiQ9D7G0. Positions 3-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 22716Binding of phosphoribosylpyrophosphateSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0462.
    GeneTreeiENSGT00550000074583.
    HOVERGENiHBG001520.
    InParanoidiQ76MX9.
    KOiK00948.
    OMAiIQYIRTN.
    OrthoDBiEOG7G4QG5.
    TreeFamiTF106366.

    Family and domain databases

    Gene3Di3.40.50.2020. 2 hits.
    HAMAPiMF_00583_B. RibP_PPkinase_B.
    InterProiIPR000842. PRib_PP_synth_CS.
    IPR029099. Pribosyltran_N.
    IPR029057. PRTase-like.
    IPR005946. Rib-P_diPkinase.
    [Graphical view]
    PfamiPF14572. Pribosyl_synth. 1 hit.
    PF13793. Pribosyltran_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
    PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D7G0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG    50
    EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK 100
    DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA 150
    VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN 200
    EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH 250
    GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI 300
    RRTHNGESVS YLFSHVPL 318
    Length:318
    Mass (Da):34,834
    Last modified:July 27, 2011 - v4
    Checksum:i46D017E969908BA0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti183 – 1831D → E in BAB26181. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025048 mRNA. Translation: BAA84686.1.
    AK009265 mRNA. Translation: BAB26181.1.
    AK011304 mRNA. Translation: BAB27530.1.
    AK089009 mRNA. Translation: BAC40697.1.
    AK165524 mRNA. Translation: BAE38237.1.
    AK165987 mRNA. Translation: BAE38503.1.
    AK166856 mRNA. Translation: BAE39073.1.
    AL672297 Genomic DNA. Translation: CAM14818.1.
    CH466616 Genomic DNA. Translation: EDL23928.1.
    CH466616 Genomic DNA. Translation: EDL23929.1.
    BC054772 mRNA. Translation: AAH54772.1.
    CCDSiCCDS30439.1.
    RefSeqiNP_067438.1. NM_021463.4.
    UniGeneiMm.287178.

    Genome annotation databases

    EnsembliENSMUST00000033809; ENSMUSP00000033809; ENSMUSG00000031432.
    GeneIDi19139.
    KEGGimmu:19139.
    UCSCiuc009uky.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025048 mRNA. Translation: BAA84686.1 .
    AK009265 mRNA. Translation: BAB26181.1 .
    AK011304 mRNA. Translation: BAB27530.1 .
    AK089009 mRNA. Translation: BAC40697.1 .
    AK165524 mRNA. Translation: BAE38237.1 .
    AK165987 mRNA. Translation: BAE38503.1 .
    AK166856 mRNA. Translation: BAE39073.1 .
    AL672297 Genomic DNA. Translation: CAM14818.1 .
    CH466616 Genomic DNA. Translation: EDL23928.1 .
    CH466616 Genomic DNA. Translation: EDL23929.1 .
    BC054772 mRNA. Translation: AAH54772.1 .
    CCDSi CCDS30439.1.
    RefSeqi NP_067438.1. NM_021463.4.
    UniGenei Mm.287178.

    3D structure databases

    ProteinModelPortali Q9D7G0.
    SMRi Q9D7G0. Positions 3-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202401. 1 interaction.
    IntActi Q9D7G0. 2 interactions.
    MINTi MINT-1858366.

    Chemistry

    BindingDBi Q9D7G0.

    PTM databases

    PhosphoSitei Q9D7G0.

    2D gel databases

    REPRODUCTION-2DPAGE Q9D7G0.

    Proteomic databases

    MaxQBi Q9D7G0.
    PaxDbi Q9D7G0.
    PRIDEi Q9D7G0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033809 ; ENSMUSP00000033809 ; ENSMUSG00000031432 .
    GeneIDi 19139.
    KEGGi mmu:19139.
    UCSCi uc009uky.2. mouse.

    Organism-specific databases

    CTDi 5631.
    MGIi MGI:97775. Prps1.

    Phylogenomic databases

    eggNOGi COG0462.
    GeneTreei ENSGT00550000074583.
    HOVERGENi HBG001520.
    InParanoidi Q76MX9.
    KOi K00948.
    OMAi IQYIRTN.
    OrthoDBi EOG7G4QG5.
    TreeFami TF106366.

    Enzyme and pathway databases

    UniPathwayi UPA00087 ; UER00172 .

    Miscellaneous databases

    ChiTaRSi PRPS1. mouse.
    NextBioi 295766.
    PROi Q9D7G0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D7G0.
    Bgeei Q9D7G0.
    CleanExi MM_PRPS1.
    Genevestigatori Q9D7G0.

    Family and domain databases

    Gene3Di 3.40.50.2020. 2 hits.
    HAMAPi MF_00583_B. RibP_PPkinase_B.
    InterProi IPR000842. PRib_PP_synth_CS.
    IPR029099. Pribosyltran_N.
    IPR029057. PRTase-like.
    IPR005946. Rib-P_diPkinase.
    [Graphical view ]
    Pfami PF14572. Pribosyl_synth. 1 hit.
    PF13793. Pribosyltran_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01251. ribP_PPkin. 1 hit.
    PROSITEi PS00114. PRPP_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse phosphoribosylpyrophosphate synthetase subunit I (PRS I)."
      Seki N., Hattori A., Hayashi A., Kozuma S., Muramatsu M., Saito T.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Lung, Thymus and Tongue.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    6. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 35-49; 244-260 AND 303-318, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.
    7. "Loss-of-function mutations in the PRPS1 gene cause a type of nonsyndromic X-linked sensorineural deafness, DFN2."
      Liu X., Han D., Li J., Han B., Ouyang X., Cheng J., Li X., Jin Z., Wang Y., Bitner-Glindzicz M., Kong X., Xu H., Kantardzhieva A., Eavey R.D., Seidman C.E., Seidman J.G., Du L.L., Chen Z.Y.
      , Dai P., Teng M., Yan D., Yuan H.
      Am. J. Hum. Genet. 86:65-71(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiPRPS1_MOUSE
    AccessioniPrimary (citable) accession number: Q9D7G0
    Secondary accession number(s): Q76MX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3