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Protein

Ribose-phosphate pyrophosphokinase 1

Gene

Prps1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by magnesium and inorganic phosphate.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase 2 (Prps2), Ribose-phosphate pyrophosphokinase 1 (Prps1)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi128MagnesiumSequence analysis1
Metal bindingi130MagnesiumSequence analysis1
Binding sitei130ATPBy similarity1
Metal bindingi139MagnesiumSequence analysis1
Metal bindingi143MagnesiumSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 101ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 1 (EC:2.7.6.1)
Alternative name(s):
Phosphoribosyl pyrophosphate synthase I
Short name:
PRS-I
Gene namesi
Name:Prps1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:97775. Prps1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001410722 – 318Ribose-phosphate pyrophosphokinase 1Add BLAST317

Proteomic databases

EPDiQ9D7G0.
MaxQBiQ9D7G0.
PaxDbiQ9D7G0.
PRIDEiQ9D7G0.

2D gel databases

REPRODUCTION-2DPAGEQ9D7G0.

PTM databases

iPTMnetiQ9D7G0.
PhosphoSitePlusiQ9D7G0.
SwissPalmiQ9D7G0.

Expressioni

Developmental stagei

Expressed in both vestibular and cochlea hair cells in early developing and postnatal mice and can also be detected in the spiral ganglion cells in at post natal day 6.1 Publication

Gene expression databases

BgeeiENSMUSG00000031432.
CleanExiMM_PRPS1.
GenevisibleiQ9D7G0. MM.

Interactioni

Subunit structurei

Homodimer. The active form is probably a hexamer composed of 3 homodimers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202401. 1 interactor.
IntActiQ9D7G0. 2 interactors.
MINTiMINT-1858366.
STRINGi10090.ENSMUSP00000033809.

Structurei

3D structure databases

ProteinModelPortaliQ9D7G0.
SMRiQ9D7G0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 227Binding of phosphoribosylpyrophosphateSequence analysisAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1448. Eukaryota.
COG0462. LUCA.
GeneTreeiENSGT00550000074583.
HOVERGENiHBG001520.
InParanoidiQ9D7G0.
KOiK00948.
OMAiESICQDS.
OrthoDBiEOG091G0ZG8.
TreeFamiTF106366.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B. 1 hit.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D7G0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG
60 70 80 90 100
EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK
110 120 130 140 150
DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA
160 170 180 190 200
VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN
210 220 230 240 250
EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH
260 270 280 290 300
GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI
310
RRTHNGESVS YLFSHVPL
Length:318
Mass (Da):34,834
Last modified:July 27, 2011 - v4
Checksum:i46D017E969908BA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti183D → E in BAB26181 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025048 mRNA. Translation: BAA84686.1.
AK009265 mRNA. Translation: BAB26181.1.
AK011304 mRNA. Translation: BAB27530.1.
AK089009 mRNA. Translation: BAC40697.1.
AK165524 mRNA. Translation: BAE38237.1.
AK165987 mRNA. Translation: BAE38503.1.
AK166856 mRNA. Translation: BAE39073.1.
AL672297 Genomic DNA. Translation: CAM14818.1.
CH466616 Genomic DNA. Translation: EDL23928.1.
CH466616 Genomic DNA. Translation: EDL23929.1.
BC054772 mRNA. Translation: AAH54772.1.
CCDSiCCDS30439.1.
RefSeqiNP_067438.1. NM_021463.4.
UniGeneiMm.287178.

Genome annotation databases

EnsembliENSMUST00000033809; ENSMUSP00000033809; ENSMUSG00000031432.
GeneIDi19139.
KEGGimmu:19139.
UCSCiuc009uky.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025048 mRNA. Translation: BAA84686.1.
AK009265 mRNA. Translation: BAB26181.1.
AK011304 mRNA. Translation: BAB27530.1.
AK089009 mRNA. Translation: BAC40697.1.
AK165524 mRNA. Translation: BAE38237.1.
AK165987 mRNA. Translation: BAE38503.1.
AK166856 mRNA. Translation: BAE39073.1.
AL672297 Genomic DNA. Translation: CAM14818.1.
CH466616 Genomic DNA. Translation: EDL23928.1.
CH466616 Genomic DNA. Translation: EDL23929.1.
BC054772 mRNA. Translation: AAH54772.1.
CCDSiCCDS30439.1.
RefSeqiNP_067438.1. NM_021463.4.
UniGeneiMm.287178.

3D structure databases

ProteinModelPortaliQ9D7G0.
SMRiQ9D7G0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202401. 1 interactor.
IntActiQ9D7G0. 2 interactors.
MINTiMINT-1858366.
STRINGi10090.ENSMUSP00000033809.

PTM databases

iPTMnetiQ9D7G0.
PhosphoSitePlusiQ9D7G0.
SwissPalmiQ9D7G0.

2D gel databases

REPRODUCTION-2DPAGEQ9D7G0.

Proteomic databases

EPDiQ9D7G0.
MaxQBiQ9D7G0.
PaxDbiQ9D7G0.
PRIDEiQ9D7G0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033809; ENSMUSP00000033809; ENSMUSG00000031432.
GeneIDi19139.
KEGGimmu:19139.
UCSCiuc009uky.2. mouse.

Organism-specific databases

CTDi5631.
MGIiMGI:97775. Prps1.

Phylogenomic databases

eggNOGiKOG1448. Eukaryota.
COG0462. LUCA.
GeneTreeiENSGT00550000074583.
HOVERGENiHBG001520.
InParanoidiQ9D7G0.
KOiK00948.
OMAiESICQDS.
OrthoDBiEOG091G0ZG8.
TreeFamiTF106366.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.

Miscellaneous databases

ChiTaRSiPrps1. mouse.
PROiQ9D7G0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031432.
CleanExiMM_PRPS1.
GenevisibleiQ9D7G0. MM.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B. 1 hit.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRPS1_MOUSE
AccessioniPrimary (citable) accession number: Q9D7G0
Secondary accession number(s): Q76MX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.