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Protein

Charged multivesicular body protein 4c

Gene

Chmp4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 4c
Alternative name(s):
Chromatin-modifying protein 4c
Short name:
CHMP4c
Gene namesi
Name:Chmp4c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913621 Chmp4c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114961 – 232Charged multivesicular body protein 4cAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210Phosphoserine; by AURKBBy similarity1

Post-translational modificationi

Phosphorylated at Ser-210 by AURKB during cytokinesis: together with ZFYVE19/ANCHR, phosphorylated CHMP4C retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9D7F7
PaxDbiQ9D7F7
PeptideAtlasiQ9D7F7
PRIDEiQ9D7F7

PTM databases

iPTMnetiQ9D7F7
PhosphoSitePlusiQ9D7F7

Expressioni

Gene expression databases

BgeeiENSMUSG00000027536
CleanExiMM_CHMP4C
ExpressionAtlasiQ9D7F7 baseline and differential
GenevisibleiQ9D7F7 MM

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Self-associates. Interacts with CHMP2A. Interacts with CHMP4A. Interacts with CHMP4B. Interacts with CHMP6. Interacts with VPS4A. Interacts with PDCD6IP; the interaction is direct (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211421, 11 interactors
IntActiQ9D7F7, 11 interactors
STRINGi10090.ENSMUSP00000029049

Structurei

3D structure databases

ProteinModelPortaliQ9D7F7
SMRiQ9D7F7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 153Intramolecular interaction with C-terminusBy similarityAdd BLAST153
Regioni154 – 232Intramolecular interaction with N-terminusBy similarityAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 45Sequence analysisAdd BLAST25
Coiled coili125 – 185Sequence analysisAdd BLAST61

Domaini

The acidic C-terminus and the basic N-terminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components (By similarity).By similarity

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1656 Eukaryota
ENOG410YE9I LUCA
GeneTreeiENSGT00390000005006
HOGENOMiHOG000209959
HOVERGENiHBG050928
InParanoidiQ9D7F7
KOiK12194
OMAiGYAAQAM
OrthoDBiEOG091G0V7X
PhylomeDBiQ9D7F7
TreeFamiTF314269

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D7F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLGKFFKG TRSSRARAAP SAQEALARLR ETEEMLAKKQ EYLENRIQRE
60 70 80 90 100
LALAKKHGSQ NKRAALQALK RKKRFEKQLT QVDGTLSTIE FQREALENSH
110 120 130 140 150
TNTEVLRNMG FAAKAMKAVH DNMDLNKIDD LMQDITEQQD IAQEISEAFS
160 170 180 190 200
QRVQFADGFD EAELLAELEE LEQEELNKKM TSLELPNVPS SSLPAQPSRK
210 220 230
ASMPSSVHRS RAASSRRAEE DDDFKQLAAW AT
Length:232
Mass (Da):26,280
Last modified:June 1, 2001 - v1
Checksum:i8227EE2A3AD1F660
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88T → I in BAB27133 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009273 mRNA Translation: BAB26186.1
AK010707 mRNA Translation: BAB27133.1
AK160189 mRNA Translation: BAE35682.1
BC115983 mRNA Translation: AAI15984.1
BC117538 mRNA Translation: AAI17539.1
CCDSiCCDS17243.1
RefSeqiNP_079795.1, NM_025519.2
UniGeneiMm.366147
Mm.440046

Genome annotation databases

EnsembliENSMUST00000029049; ENSMUSP00000029049; ENSMUSG00000027536
GeneIDi66371
KEGGimmu:66371
UCSCiuc008opu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCHM4C_MOUSE
AccessioniPrimary (citable) accession number: Q9D7F7
Secondary accession number(s): Q149R2, Q3TVD7, Q9CWH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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