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Protein

Nicotinamide riboside kinase 2

Gene

Nmrk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). Reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. May play a role in the regulation of terminal myogenesis (By similarity).By similarity1 Publication

Catalytic activityi

ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide.By similarity
ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide.By similarity

Pathwayi: NAD(+) biosynthesis

This protein is involved in the pathway NAD(+) biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumBy similarity1
Active sitei35Proton acceptorBy similarity1
Metal bindingi35MagnesiumBy similarity1
Binding sitei130ATPBy similarity1
Binding sitei131SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 17ATPBy similarity9
Nucleotide bindingi134 – 136ATPBy similarity3
Nucleotide bindingi174 – 176ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • NAD biosynthetic process Source: UniProtKB-UniPathway
  • negative regulation of myoblast differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide riboside kinase 2 (EC:2.7.1.22By similarity)
Short name:
NRK 2
Short name:
NmR-K 2
Alternative name(s):
Integrin beta-1-binding protein 3
Muscle integrin-binding protein
Short name:
MIBP
Nicotinic acid riboside kinase 2 (EC:2.7.1.173By similarity)
Ribosylnicotinamide kinase 2
Short name:
RNK 2
Ribosylnicotinic acid kinase 2
Gene namesi
Name:Nmrk2
Synonyms:Itgb1bp3, Nrk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1916814. Nmrk2.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158951 – 195Nicotinamide riboside kinase 2Add BLAST195

Proteomic databases

PaxDbiQ9D7C9.
PRIDEiQ9D7C9.

PTM databases

PhosphoSitePlusiQ9D7C9.

Expressioni

Tissue specificityi

Expressed in skeletal muscle (at protein level).1 Publication

Inductioni

Down-regulated during myoblast differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000004939.
CleanExiMM_ITGB1BP3.
GenevisibleiQ9D7C9. MM.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with ITGB1 alone or when associated with alpha-7, but not with alpha-5.By similarity

Protein-protein interaction databases

BioGridi213534. 1 interactor.
STRINGi10090.ENSMUSP00000005069.

Structurei

3D structure databases

ProteinModelPortaliQ9D7C9.
SMRiQ9D7C9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38Substrate bindingBy similarity4
Regioni54 – 55Substrate bindingBy similarity2
Regioni136 – 137Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the uridine kinase family. NRK subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IR10. Eukaryota.
ENOG4111VGZ. LUCA.
GeneTreeiENSGT00510000046782.
HOGENOMiHOG000043899.
HOVERGENiHBG052669.
InParanoidiQ9D7C9.
KOiK10524.
OMAiQKFARAH.
OrthoDBiEOG091G0JV5.
PhylomeDBiQ9D7C9.
TreeFamiTF105395.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D7C9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIIGIGGV TNGGKTTLTN SLLKALPNCC VIHQDDFFKP QDQIAVGEDG
60 70 80 90 100
FKQWDVLESL DMETMLSTVQ AWVKDPHKFA RAHGVSLQSG ASDTHVLLLE
110 120 130 140 150
GFLLYSYRPL VDLYSQRYFL TVPYEECKRR RRSRTYMVPD PPGLFDGHVW
160 170 180 190
PMYQKYRREM EQDGVEVVYL DGMKSPEGLF HQVLEDIQNR LLNTS
Length:195
Mass (Da):22,375
Last modified:June 1, 2001 - v1
Checksum:i83B0FB3F52738455
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009352 mRNA. Translation: BAB26235.1.
AK137483 mRNA. Translation: BAE23375.1.
BC119244 mRNA. Translation: AAI19245.1.
BC119246 mRNA. Translation: AAI19247.1.
CCDSiCCDS24046.1.
RefSeqiNP_081396.1. NM_027120.2.
UniGeneiMm.437211.

Genome annotation databases

EnsembliENSMUST00000005069; ENSMUSP00000005069; ENSMUSG00000004939.
GeneIDi69564.
KEGGimmu:69564.
UCSCiuc007ggn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009352 mRNA. Translation: BAB26235.1.
AK137483 mRNA. Translation: BAE23375.1.
BC119244 mRNA. Translation: AAI19245.1.
BC119246 mRNA. Translation: AAI19247.1.
CCDSiCCDS24046.1.
RefSeqiNP_081396.1. NM_027120.2.
UniGeneiMm.437211.

3D structure databases

ProteinModelPortaliQ9D7C9.
SMRiQ9D7C9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213534. 1 interactor.
STRINGi10090.ENSMUSP00000005069.

PTM databases

PhosphoSitePlusiQ9D7C9.

Proteomic databases

PaxDbiQ9D7C9.
PRIDEiQ9D7C9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005069; ENSMUSP00000005069; ENSMUSG00000004939.
GeneIDi69564.
KEGGimmu:69564.
UCSCiuc007ggn.1. mouse.

Organism-specific databases

CTDi27231.
MGIiMGI:1916814. Nmrk2.

Phylogenomic databases

eggNOGiENOG410IR10. Eukaryota.
ENOG4111VGZ. LUCA.
GeneTreeiENSGT00510000046782.
HOGENOMiHOG000043899.
HOVERGENiHBG052669.
InParanoidiQ9D7C9.
KOiK10524.
OMAiQKFARAH.
OrthoDBiEOG091G0JV5.
PhylomeDBiQ9D7C9.
TreeFamiTF105395.

Enzyme and pathway databases

UniPathwayiUPA00253.

Miscellaneous databases

PROiQ9D7C9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004939.
CleanExiMM_ITGB1BP3.
GenevisibleiQ9D7C9. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNRK2_MOUSE
AccessioniPrimary (citable) accession number: Q9D7C9
Secondary accession number(s): Q0VEG2, Q3UV96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.