Reviewed, UniProtKB/Swiss-Prot Q9D7B7 (GPX8_MOUSE)

Names and origin

Protein names

Recommended name:
    Probable glutathione peroxidase 8
      Short name=GSHPx-8
      Short name=GPx-8
    EC=1.11.1.9

Gene names

Name:

Gpx8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Probable glutathione peroxidase 8		PRO_0000317757
Regions
	Transmembrane	18 – 40	23	Potential
Sites
	Active site	79	1	By similarity
Experimental info
	Sequence conflict	88	1	T → I in AAH19664. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9D7B7-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8DA93ED11B4ECF21	FASTA	209	24,148
	10 20 30 40 50 60 MEPFAAYPLK CSGPKAKIFA VLLSMVLCTV MLFLLQLKFL KPRTNSFYSF EVKDAKGRTV 70 80 90 100 110 120 SLEKFKGKAS LVVNVASDCR FTDKSYQTLR ELHKEFGPYH FNVLAFPCNQ FGESEPKSSK 130 140 150 160 170 180 EVESFARQNY GVTFPIFHKI KILGPEAEPA FRFIVDSSKK EPRWNFWKYL VNPEGQVVKF 190 200 WRPEEPLEAI RPHVSQMIGQ IILKKKEDL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Tongue.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK009378 mRNA. Translation: BAB26254.1. BC019664 mRNA. Translation: AAH19664.1.
IPI	IPI00110128.
RefSeq	NP_081403.1.
UniGene	Mm.12715
3D structure databases
HSSP	HSSP built from PDB template 2F8A based on UniProtKB P07203.
SMR	Q9D7B7. Positions 39-209.
ModBase	Search...
Proteomic databases
PRIDE	Q9D7B7.
Genome annotation databases
Ensembl	ENSMUST00000022282; ENSMUSP00000022282; ENSMUSG00000021760; Mus musculus. [Genome view]
GeneID	69590.
KEGG	mmu:69590.
NMPDR	fig|10090.3.peg.28433.
UCSC	uc007rxb.1. mouse.
Organism-specific databases
CTD	69590.
MGI	MGI:1916840. 2310016C16Rik.
Phylogenomic databases
eggNOG	roNOG12665.
HOGENOM	HBG648990.
HOVERGEN	Q9D7B7.
InParanoid	Q9D7B7.
OMA	FGPFHFS.
OrthoDB	EOG9M94BW.
PhylomeDB	Q9D7B7.
Enzyme and pathway databases
BRENDA	1.11.1.9. 244.
Gene expression databases
ArrayExpress	Q9D7B7.
Bgee	Q9D7B7.
Genevestigator	Q9D7B7.
Family and domain databases
InterPro	IPR013376. Glut_perox_Gpx7. IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
TIGRFAMs	TIGR02540. gpx7. 1 hit.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. False negative. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	329860.
SOURCE	Search...

Entry information

Entry name

GPX8_MOUSE

Accession

Primary (citable) accession number: Q9D7B7
Secondary accession number(s): Q8VE68

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)