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Protein

Isobutyryl-CoA dehydrogenase, mitochondrial

Gene

Acad8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has very high activity toward isobutyryl-CoA. Is an isobutyryl-CoA dehydrogenase that functions in valine catabolism (By similarity).By similarity

Catalytic activityi

Isobutyryl-CoA + ETF = methylacrylyl-CoA + reduced ETF.

Cofactori

FADBy similarity

Pathwayi: L-valine degradation

This protein is involved in the pathway L-valine degradation, which is part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the pathway L-valine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165Substrate; via carbonyl oxygenBy similarity1
Binding sitei300FADBy similarity1
Binding sitei300FAD; shared with dimeric partnerBy similarity1
Active sitei396Proton acceptorBy similarity1
Binding sitei397Substrate; via amide nitrogenBy similarity1
Binding sitei408SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi155 – 165FADBy similarityAdd BLAST11
Nucleotide bindingi156 – 165FADBy similarity10
Nucleotide bindingi189 – 191FADBy similarity3
Nucleotide bindingi310 – 311FAD; shared with dimeric partnerBy similarity2
Nucleotide bindingi369 – 375FADBy similarity7
Nucleotide bindingi398 – 400FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Branched-chain amino acid catabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00362.

Names & Taxonomyi

Protein namesi
Recommended name:
Isobutyryl-CoA dehydrogenase, mitochondrial (EC:1.3.99.-)
Alternative name(s):
Acyl-CoA dehydrogenase family member 8
Short name:
ACAD-8
Gene namesi
Name:Acad8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1914198. Acad8.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20MitochondrionSequence analysisAdd BLAST20
ChainiPRO_000000052321 – 413Isobutyryl-CoA dehydrogenase, mitochondrialAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysine; alternateCombined sources1
Modified residuei48N6-succinyllysine; alternateCombined sources1
Modified residuei211N6-succinyllysineCombined sources1
Modified residuei229N6-acetyllysineCombined sources1
Modified residuei269N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9D7B6.
MaxQBiQ9D7B6.
PaxDbiQ9D7B6.
PeptideAtlasiQ9D7B6.
PRIDEiQ9D7B6.

PTM databases

iPTMnetiQ9D7B6.
PhosphoSitePlusiQ9D7B6.
SwissPalmiQ9D7B6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031969.
CleanExiMM_ACAD8.

Interactioni

Subunit structurei

Homotetramer, formed by a dimer of dimers. Subunit of the large multiprotein complex ARC/DRIP (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9D7B6. 2 interactors.
MINTiMINT-1861259.
STRINGi10090.ENSMUSP00000054370.

Structurei

3D structure databases

ProteinModelPortaliQ9D7B6.
SMRiQ9D7B6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni272 – 275Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0140. Eukaryota.
COG1960. LUCA.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ9D7B6.
KOiK11538.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D7B6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMLRSGYRR FGCLRAALKS LAQTHHRSIT FCIDPSLGLN EEQKGFQKVA
60 70 80 90 100
FDFAAREMAP NMAEWDQKEL FPVDVMRKAA QLGFGGVYVR TDVGGSGLSR
110 120 130 140 150
LDTSVIFEAL ATGCTSTTAY ISIHNMCAWM IDSFGNEEQR HKFCPPLCTM
160 170 180 190 200
EKFASYCLTE PGSGSDAASL LTSAKQQGDH YILNGSKAFI SGGGESDIYV
210 220 230 240 250
VMCRTGGSGA KGISCIVVEK GTPGLSFGKK EKKVGWNSQP TRAVIFEDCA
260 270 280 290 300
VPVANRIGTE GQGFLIAMKG LNGGRINVAS CSLGAAHASV ILTQEHLKVR
310 320 330 340 350
KQFGAPLARS QYLQFQLADM ATKLVASRLM IRTAAVALQE EREDAVALCS
360 370 380 390 400
MAKLFATEEC FAICNQALQM HGGYGYLKDY AVQQYMRDSR VHQILEGSNE
410
VMRMLISRNL LQD
Length:413
Mass (Da):45,020
Last modified:May 16, 2003 - v2
Checksum:i13DED42D4BD51231
GO

Sequence cautioni

The sequence BAC26664 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207G → E in BAB26255 (PubMed:16141072).Curated1
Sequence conflicti207G → E in BAB31764 (PubMed:16141072).Curated1
Sequence conflicti207G → E in BAC26664 (PubMed:16141072).Curated1
Sequence conflicti207G → E in BAC34521 (PubMed:16141072).Curated1
Sequence conflicti207G → E in BAC36757 (PubMed:16141072).Curated1
Sequence conflicti254A → P in BAC34521 (PubMed:16141072).Curated1
Sequence conflicti413D → RLAPKLESLAWSLCAAGISL DAPMGCSVQVNHEVDQRAEL F in BAB31764 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009379 mRNA. Translation: BAB26255.2.
AK019502 mRNA. Translation: BAB31764.1.
AK029897 mRNA. Translation: BAC26664.1. Different initiation.
AK051091 mRNA. Translation: BAC34521.1.
AK077335 mRNA. Translation: BAC36757.1.
BC037644 mRNA. Translation: AAH37644.1.
CCDSiCCDS40567.1.
RefSeqiNP_080138.2. NM_025862.2.
UniGeneiMm.289244.

Genome annotation databases

GeneIDi66948.
KEGGimmu:66948.
UCSCiuc009opy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009379 mRNA. Translation: BAB26255.2.
AK019502 mRNA. Translation: BAB31764.1.
AK029897 mRNA. Translation: BAC26664.1. Different initiation.
AK051091 mRNA. Translation: BAC34521.1.
AK077335 mRNA. Translation: BAC36757.1.
BC037644 mRNA. Translation: AAH37644.1.
CCDSiCCDS40567.1.
RefSeqiNP_080138.2. NM_025862.2.
UniGeneiMm.289244.

3D structure databases

ProteinModelPortaliQ9D7B6.
SMRiQ9D7B6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D7B6. 2 interactors.
MINTiMINT-1861259.
STRINGi10090.ENSMUSP00000054370.

PTM databases

iPTMnetiQ9D7B6.
PhosphoSitePlusiQ9D7B6.
SwissPalmiQ9D7B6.

Proteomic databases

EPDiQ9D7B6.
MaxQBiQ9D7B6.
PaxDbiQ9D7B6.
PeptideAtlasiQ9D7B6.
PRIDEiQ9D7B6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66948.
KEGGimmu:66948.
UCSCiuc009opy.2. mouse.

Organism-specific databases

CTDi27034.
MGIiMGI:1914198. Acad8.

Phylogenomic databases

eggNOGiKOG0140. Eukaryota.
COG1960. LUCA.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ9D7B6.
KOiK11538.

Enzyme and pathway databases

UniPathwayiUPA00362.

Miscellaneous databases

ChiTaRSiAcad8. mouse.
PROiQ9D7B6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031969.
CleanExiMM_ACAD8.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACAD8_MOUSE
AccessioniPrimary (citable) accession number: Q9D7B6
Secondary accession number(s): Q8BK36
, Q8BKQ8, Q8CFY9, Q9D2L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 16, 2003
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.