Q9D7B1 (DUS2L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
EC=1.3.1.-

Alternative name(s):
tRNA-dihydrouridine synthase 2-like

Gene names

Name:

Dus2l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.
Cofactor	FAD By similarity.
Subunit structure	Interacts with EPRS By similarity.
Subcellular location	Cytoplasm By similarity. Endoplasmic reticulum By similarity. Note: Mainly at the endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the dus family. Dus2 subfamily. Contains 1 DRBM (double-stranded RNA-binding) domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Endoplasmic reticulum
Ligand	FAD Flavoprotein RNA-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: MGI
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 493

493

tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

PRO_0000162158

Regions

Domain

369 – 436

68

DRBM

Secondary structure

1

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493

Helix Strand Turn

Details...

Beta strand

350 – 355

6

Helix

361 – 363

3

Helix

370 – 380

11

Beta strand

390 – 392

3

Beta strand

394 – 396

3

Beta strand

399 – 405

7

Beta strand

408 – 414

7

Beta strand

416 – 418

3

Helix

419 – 434

16

Beta strand

437 – 439

3

Turn

440 – 442

3

Sequences

	Sequence		Length	Mass (Da)
	Q9D7B1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B66BDCB06B096299	FASTA	493	55,325
	10 20 30 40 50 60 MIVNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKML QCKRVVNEVL 70 80 90 100 110 120 STVDFVAPDD RVVFRTCERE QSRVVFQMGT SDAERALAVA RLVENDVAGI DVNMGCPKEY 130 140 150 160 170 180 STKGGMGAAL LSDPDKIEKI LSTLVKGTHR PVTCKIRILP SLEDTLNLVK RIERTGISAI 190 200 210 220 230 240 AVHGRNRDER PQHPVSCEVI RAIAETLSIP VIANGGSHDH IQQHVDIEDF RQATAASSVM 250 260 270 280 290 300 VARAAMWNPS IFLKDGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL 310 320 330 340 350 360 LHAAQSSQEI CEAFGLGAFY EETIRELDAR RADLLAKTPE AVEEPAEDTS GIIKMAIRFD 370 380 390 400 410 420 RRAYPPQITP KMCLLEWCRR EKLPQPVYET VQRTIDRMFC SVVTVAEQKY QSTLWDKSKK 430 440 450 460 470 480 LAEQTAAIVC LRSQGLPEGR LGEESPSLNK RKREAPDQDP GGPRVQEPAL PGEICKKPFV 490 TLDSSEENLL EGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Tongue.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 205-493. Strain: C57BL/6. Tissue: Brain.
[3]	"Solution structure of the DSRBD from hypothetical protein BAB26260." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 350-464.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK009391 mRNA. Translation: BAB26260.1.
BC058431 mRNA. Translation: AAH58431.1.

IPI

IPI00110119.

RefSeq

NP_079794.1. NM_025518.3.

UniGene

Mm.287500.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WHN	NMR	-	A	350-464	[»]

ProteinModelPortal

Q9D7B1.

SMR

Q9D7B1. Positions 88-253, 352-464.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9D7B1.

PTM databases

PhosphoSite

Q9D7B1.

Proteomic databases

PRIDE

Q9D7B1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000034375; ENSMUSP00000034375; ENSMUSG00000031901.

GeneID

66369.

KEGG

mmu:66369.

Organism-specific databases

CTD

54920.

MGI

MGI:1913619. Dus2l.

Phylogenomic databases

GeneTree

ENSGT00550000075019.

HOGENOM

HBG396464.

HOVERGEN

HBG079551.

InParanoid

Q9D7B1.

OMA

YHNKLIL.

OrthoDB

EOG4MW85W.

PhylomeDB

Q9D7B1.

Gene expression databases

ArrayExpress

Q9D7B1.

Bgee

Q9D7B1.

Genevestigator

Q9D7B1.

GermOnline

ENSMUSG00000031901. Mus musculus.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit.

KO

K05543.

PANTHER

PTHR11082. Du_synth. 1 hit.

Pfam

PF00035. dsrm. 1 hit.
PF01207. Dus. 1 hit.
[Graphical view]

SMART

SM00358. DSRM. 1 hit.
[Graphical view]

PROSITE

PS50137. DS_RBD. False negative.
PS01136. UPF0034. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

321469.

SOURCE

Search...

Entry information

Entry name

DUS2L_MOUSE

Accession

Primary (citable) accession number: Q9D7B1
Secondary accession number(s): Q6PDX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program