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Protein

Mitotic spindle assembly checkpoint protein MAD2B

Gene

Mad2l2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, DNA damage, DNA repair, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-110312 Translesion synthesis by REV1
R-MMU-5655862 Translesion synthesis by POLK
R-MMU-5656121 Translesion synthesis by POLI

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle assembly checkpoint protein MAD2B
Alternative name(s):
Mitotic arrest deficient 2-like protein 2
Short name:
MAD2-like protein 2
Gene namesi
Name:Mad2l2
Synonyms:Mad2b, Rev7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1919140 Mad2l2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001261201 – 211Mitotic spindle assembly checkpoint protein MAD2BAdd BLAST211

Proteomic databases

MaxQBiQ9D752
PaxDbiQ9D752
PRIDEiQ9D752

PTM databases

PhosphoSitePlusiQ9D752

Expressioni

Gene expression databases

BgeeiENSMUSG00000029003
CleanExiMM_MAD2L2
ExpressionAtlasiQ9D752 baseline and differential
GenevisibleiQ9D752 MM

Interactioni

Subunit structurei

Homooligomer. Interacts with ADAM9. Interacts with CHAMP1. Interacts with FZR1 (in complex with the anaphase promoting complex APC). May interact with CDC20. Interacts with RAN. Interacts with ELK1; the interaction is direct and recruits MAD2L2 to ELK1-specific promoters. May interact with the JNK kinases MAPK8 and/or MAPK9 to stimulate ELK1 phosphorylation and transcriptional activity upon DNA damage. Interacts with TCF7L2; prevents its binding to promoters and negatively modulates its transcriptional activity. Interacts with YY1AP1. Interacts with PRCC; the interaction is direct. Interacts with POGZ (By similarity). Interacts with REV1. Heterodimer with REV3L. This dimer forms the minimal DNA polymerase zeta complex (Pol-zeta2), with REV3L bearing DNA polymerase catalytic activity, although its activity is very low in this context. Component of the tetrameric Pol-zeta complex (Pol-zeta4), which consists of REV3L, MAD2L2, POLD2 and POLD3; Pol-zeta4 is the fully active form of DNA polymerase zeta (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215010, 31 interactors
IntActiQ9D752, 28 interactors
STRINGi10090.ENSMUSP00000030860

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Turni6 – 8Combined sources3
Helixi11 – 33Combined sources23
Helixi39 – 41Combined sources3
Beta strandi42 – 47Combined sources6
Beta strandi50 – 55Combined sources6
Helixi58 – 76Combined sources19
Beta strandi80 – 88Combined sources9
Beta strandi94 – 103Combined sources10
Beta strandi109 – 111Combined sources3
Turni112 – 116Combined sources5
Helixi117 – 131Combined sources15
Helixi133 – 136Combined sources4
Beta strandi145 – 154Combined sources10
Helixi157 – 162Combined sources6
Beta strandi171 – 173Combined sources3
Helixi176 – 179Combined sources4
Beta strandi182 – 192Combined sources11
Beta strandi198 – 208Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FJOX-ray2.72C1-210[»]
ProteinModelPortaliQ9D752
SMRiQ9D752
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 203HORMAPROSITE-ProRule annotationAdd BLAST191

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 155Mediates interaction with REV1 and REV3L and homodimerizationBy similarityAdd BLAST135

Phylogenomic databases

eggNOGiKOG3186 Eukaryota
ENOG4111I7Q LUCA
GeneTreeiENSGT00500000044946
HOGENOMiHOG000231083
HOVERGENiHBG052443
InParanoidiQ9D752
KOiK13728
OMAiPWIVADE
OrthoDBiEOG091G0KY2
PhylomeDBiQ9D752
TreeFamiTF101085

Family and domain databases

Gene3Di3.30.900.10, 1 hit
InterProiView protein in InterPro
IPR003511 HORMA_dom
IPR036570 HORMA_dom_sf
PfamiView protein in Pfam
PF02301 HORMA, 1 hit
SUPFAMiSSF56019 SSF56019, 1 hit
PROSITEiView protein in PROSITE
PS50815 HORMA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTRQDLN FGQVVADVLS EFLEVAVHLI LYVREVYPVG IFQKRKKYNV
60 70 80 90 100
PVQMSCHPEL NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF
110 120 130 140 150
EITQPPLLSI NSDSLLSHVE QLLRAFILKI SVCDAVLDHN PPGCTFTVLV
160 170 180 190 200
HTREAATRNM EKIQVIKDFP WILADEQDVH MHDPRLIPLK TMTSDILKMQ
210
LYVEERAHKN S
Length:211
Mass (Da):24,402
Last modified:April 26, 2004 - v2
Checksum:i0E47D0D8AC28AB5C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193T → M in BAB26376 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009587 mRNA Translation: BAB26376.1
AK150216 mRNA Translation: BAE29386.1
AK165627 mRNA Translation: BAE38303.1
BC011282 mRNA Translation: AAH11282.1
BC071264 mRNA Translation: AAH71264.1
CCDSiCCDS18932.1
RefSeqiNP_001292349.1, NM_001305420.1
NP_082261.2, NM_027985.3
XP_006539246.1, XM_006539183.2
XP_006539249.1, XM_006539186.1
XP_006539250.1, XM_006539187.2
UniGeneiMm.9648

Genome annotation databases

EnsembliENSMUST00000030860; ENSMUSP00000030860; ENSMUSG00000029003
ENSMUST00000084129; ENSMUSP00000081146; ENSMUSG00000029003
GeneIDi71890
KEGGimmu:71890
UCSCiuc008vub.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMD2L2_MOUSE
AccessioniPrimary (citable) accession number: Q9D752
Secondary accession number(s): Q3TMY6, Q91VP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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