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Protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial

Gene

Alkbh7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity (By similarity). Involved in fatty acid metabolism.By similarity1 Publication

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi121 – 1211Iron; catalyticBy similarity
Metal bindingi123 – 1231Iron; catalyticBy similarity
Binding sitei165 – 1651Alpha-ketoglutarateBy similarity
Metal bindingi177 – 1771Iron; catalyticBy similarity
Binding sitei203 – 2031Alpha-ketoglutarateBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • fatty acid metabolic process Source: UniProtKB
  • regulation of lipid storage Source: UniProtKB
  • regulation of mitochondrial membrane permeability involved in programmed necrotic cell death Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Necrosis

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (EC:1.14.11.-)
Alternative name(s):
Alkylated DNA repair protein alkB homolog 7
Gene namesi
Name:Alkbh7
Synonyms:Spata11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1913650. Alkbh7.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increased body weight and body fat, a phenotype amplified under high-fat diet.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323MitochondrionSequence analysisAdd
BLAST
Chaini24 – 221198Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrialPRO_0000239289Add
BLAST

Proteomic databases

EPDiQ9D6Z0.
MaxQBiQ9D6Z0.
PaxDbiQ9D6Z0.
PRIDEiQ9D6Z0.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ9D6Z0.
CleanExiMM_ALKBH7.
GenevisibleiQ9D6Z0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002737.

Structurei

3D structure databases

ProteinModelPortaliQ9D6Z0.
SMRiQ9D6Z0. Positions 17-206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 1993Alpha-ketoglutarate bindingBy similarity

Sequence similaritiesi

Belongs to the alkB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4176. Eukaryota.
COG3145. LUCA.
GeneTreeiENSGT00390000014585.
HOGENOMiHOG000018442.
HOVERGENiHBG054357.
InParanoidiQ9D6Z0.
KOiK10769.
OMAiRYEKSHW.
OrthoDBiEOG7M0NT9.
PhylomeDBiQ9D6Z0.
TreeFamiTF314197.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR032870. ALKBH7.
[Graphical view]
PANTHERiPTHR21052:SF0. PTHR21052:SF0. 1 hit.
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6Z0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSRRLAMR LLSGCAWVRG SDSAVLGRLR DEAVVHPGFL SQEEEDTLTR
60 70 80 90 100
ELEPQLRRRR YEYDHWDAAI HGFRETEKSC WSDASQVILQ RVRAAAFGPD
110 120 130 140 150
QSLLSPVHVL DLEPRGYIKP HVDSVKFCGS TIAGLSLLSP SVMKLVHTQE
160 170 180 190 200
PEQWLELLLE PGSLYILRGS ARYDFSHEIL RDEESFFGEH RVPRGRRISV
210 220
ICRSLPEGMG PGRPEEPPPA C
Length:221
Mass (Da):24,970
Last modified:June 1, 2001 - v1
Checksum:i7961509733692740
GO
Isoform 2 (identifier: Q9D6Z0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-126: Missing.

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,532
Checksum:i0B8B34C20A3B7B1B
GO

Sequence cautioni

The sequence AAH29677.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911R → L in BAB24999 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei69 – 12658Missing in isoform 2. 1 PublicationVSP_019132Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007380 mRNA. Translation: BAB24999.1.
AK009812 mRNA. Translation: BAB26517.1.
AK010930 mRNA. Translation: BAB27274.1.
GL456179 Genomic DNA. No translation available.
BC029677 mRNA. Translation: AAH29677.1. Different initiation.
CCDSiCCDS28921.1. [Q9D6Z0-1]
CCDS57105.1. [Q9D6Z0-2]
RefSeqiNP_079814.1. NM_025538.3. [Q9D6Z0-1]
NP_081648.1. NM_027372.1. [Q9D6Z0-2]
UniGeneiMm.196150.

Genome annotation databases

EnsembliENSMUST00000002737; ENSMUSP00000002737; ENSMUSG00000002661. [Q9D6Z0-1]
ENSMUST00000074141; ENSMUSP00000073775; ENSMUSG00000002661. [Q9D6Z0-2]
GeneIDi66400.
KEGGimmu:66400.
UCSCiuc008ddn.2. mouse. [Q9D6Z0-1]
uc012avz.1. mouse. [Q9D6Z0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007380 mRNA. Translation: BAB24999.1.
AK009812 mRNA. Translation: BAB26517.1.
AK010930 mRNA. Translation: BAB27274.1.
GL456179 Genomic DNA. No translation available.
BC029677 mRNA. Translation: AAH29677.1. Different initiation.
CCDSiCCDS28921.1. [Q9D6Z0-1]
CCDS57105.1. [Q9D6Z0-2]
RefSeqiNP_079814.1. NM_025538.3. [Q9D6Z0-1]
NP_081648.1. NM_027372.1. [Q9D6Z0-2]
UniGeneiMm.196150.

3D structure databases

ProteinModelPortaliQ9D6Z0.
SMRiQ9D6Z0. Positions 17-206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002737.

Proteomic databases

EPDiQ9D6Z0.
MaxQBiQ9D6Z0.
PaxDbiQ9D6Z0.
PRIDEiQ9D6Z0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002737; ENSMUSP00000002737; ENSMUSG00000002661. [Q9D6Z0-1]
ENSMUST00000074141; ENSMUSP00000073775; ENSMUSG00000002661. [Q9D6Z0-2]
GeneIDi66400.
KEGGimmu:66400.
UCSCiuc008ddn.2. mouse. [Q9D6Z0-1]
uc012avz.1. mouse. [Q9D6Z0-2]

Organism-specific databases

CTDi84266.
MGIiMGI:1913650. Alkbh7.

Phylogenomic databases

eggNOGiKOG4176. Eukaryota.
COG3145. LUCA.
GeneTreeiENSGT00390000014585.
HOGENOMiHOG000018442.
HOVERGENiHBG054357.
InParanoidiQ9D6Z0.
KOiK10769.
OMAiRYEKSHW.
OrthoDBiEOG7M0NT9.
PhylomeDBiQ9D6Z0.
TreeFamiTF314197.

Miscellaneous databases

NextBioi321567.
PROiQ9D6Z0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6Z0.
CleanExiMM_ALKBH7.
GenevisibleiQ9D6Z0. MM.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR032870. ALKBH7.
[Graphical view]
PANTHERiPTHR21052:SF0. PTHR21052:SF0. 1 hit.
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Liver, Pancreas and Tongue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-221 (ISOFORM 1).
    Tissue: Eye.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver and Testis.
  5. Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiALKB7_MOUSE
AccessioniPrimary (citable) accession number: Q9D6Z0
Secondary accession number(s): Q8K1H3, Q9CY41, Q9D942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: March 16, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.