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Protein

1,4-alpha-glucan-branching enzyme

Gene

Gbe1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells (By similarity).By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571NucleophileBy similarity
Active sitei412 – 4121Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

ReactomeiREACT_292921. Glycogen synthesis.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Brancher enzyme
Glycogen-branching enzyme
Gene namesi
Name:Gbe1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1921435. Gbe1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Glycogen storage disease

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 7027011,4-alpha-glucan-branching enzymePRO_0000188776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei173 – 1731PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9D6Y9.
PaxDbiQ9D6Y9.
PRIDEiQ9D6Y9.

PTM databases

PhosphoSiteiQ9D6Y9.

Expressioni

Gene expression databases

BgeeiQ9D6Y9.
CleanExiMM_GBE1.
ExpressionAtlasiQ9D6Y9. baseline and differential.
GenevisibleiQ9D6Y9. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9D6Y9. 1 interaction.
MINTiMINT-4127309.
STRINGi10090.ENSMUSP00000023393.

Structurei

3D structure databases

ProteinModelPortaliQ9D6Y9.
SMRiQ9D6Y9. Positions 44-699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
GeneTreeiENSGT00390000017040.
HOGENOMiHOG000175159.
HOVERGENiHBG051734.
InParanoidiQ9D6Y9.
KOiK00700.
OMAiGMLEFDR.
OrthoDBiEOG7Q5HCP.
PhylomeDBiQ9D6Y9.
TreeFamiTF300783.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D6Y9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAAPAAG ETGPDARLEA ALADVPELAR LLEIDPYLKP FAADFQRRYK
60 70 80 90 100
KFSQVLHDIG ENEGGIDKFS RGYESFGIHR CSDGGIYCKE WAPGAEGVFL
110 120 130 140 150
TGEFSGWNPF SHPYKKLEYG KWELYIPPKQ NKSPLIPHGS KLKVVITSKS
160 170 180 190 200
GEILYRISPW AKYVVRENNN VNYDWIHWAP EDPYKFKHSR PKKPRSLRIY
210 220 230 240 250
ESHVGISSHE GKIASYKHFT SNVLPRIKDL GYNCIQLMAI MEHAYYASFG
260 270 280 290 300
YQITSFFAAS SRYGTPEELK ELVDTAHSMG IVVLLDVVHS HASKNSEDGL
310 320 330 340 350
NMFDGTDSCY FHSGPRGTHD LWDSRLFIYS SWEVLRFLLS NIRWWLEEYC
360 370 380 390 400
FDGFRFDGVT SMLYHHHGMG QGFSGDYNEY FGLQVDEDAL IYLMLANHLA
410 420 430 440 450
HTLYPDSITI AEDVSGMPAL CSPTSQGGGG FDYRLAMAIP DKWIQLLKEF
460 470 480 490 500
KDEDWNMGNI VYTLTNRRYL EKCVAYAESH DQALVGDKTL AFWLMDAEMY
510 520 530 540 550
TNMSVLAPFT PVIDRGIQLH KMIRLITHGL GGEGYLNFMG NEFGHPEWLD
560 570 580 590 600
FPRKGNNESY HYARRQFNLT DDDLLRYKFL NNFDRDMNRL EERCGWLSAP
610 620 630 640 650
QAYVSEKHEA NKTITFERAG LLFIFNFHPS KSYTDYRVGT ATPGKFKIVL
660 670 680 690 700
DSDAAEYGGH QRLDHNTNYF AEAFEHNGRP YSLLVYIPSR VALILQNVDL

QN
Length:702
Mass (Da):80,364
Last modified:June 1, 2001 - v1
Checksum:iBE2284A5CED7C060
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009815 mRNA. Translation: BAB26519.1.
AK050365 mRNA. Translation: BAC34210.1.
BC017541 mRNA. Translation: AAH17541.1.
RefSeqiNP_083079.1. NM_028803.4.
UniGeneiMm.396102.

Genome annotation databases

EnsembliENSMUST00000163832; ENSMUSP00000132603; ENSMUSG00000022707.
GeneIDi74185.
KEGGimmu:74185.
UCSCiuc007zqu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009815 mRNA. Translation: BAB26519.1.
AK050365 mRNA. Translation: BAC34210.1.
BC017541 mRNA. Translation: AAH17541.1.
RefSeqiNP_083079.1. NM_028803.4.
UniGeneiMm.396102.

3D structure databases

ProteinModelPortaliQ9D6Y9.
SMRiQ9D6Y9. Positions 44-699.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D6Y9. 1 interaction.
MINTiMINT-4127309.
STRINGi10090.ENSMUSP00000023393.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

PTM databases

PhosphoSiteiQ9D6Y9.

Proteomic databases

MaxQBiQ9D6Y9.
PaxDbiQ9D6Y9.
PRIDEiQ9D6Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000163832; ENSMUSP00000132603; ENSMUSG00000022707.
GeneIDi74185.
KEGGimmu:74185.
UCSCiuc007zqu.2. mouse.

Organism-specific databases

CTDi2632.
MGIiMGI:1921435. Gbe1.

Phylogenomic databases

eggNOGiCOG0296.
GeneTreeiENSGT00390000017040.
HOGENOMiHOG000175159.
HOVERGENiHBG051734.
InParanoidiQ9D6Y9.
KOiK00700.
OMAiGMLEFDR.
OrthoDBiEOG7Q5HCP.
PhylomeDBiQ9D6Y9.
TreeFamiTF300783.

Enzyme and pathway databases

UniPathwayiUPA00164.
ReactomeiREACT_292921. Glycogen synthesis.

Miscellaneous databases

ChiTaRSiGbe1. mouse.
NextBioi340028.
PROiQ9D6Y9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6Y9.
CleanExiMM_GBE1.
ExpressionAtlasiQ9D6Y9. baseline and differential.
GenevisibleiQ9D6Y9. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiGLGB_MOUSE
AccessioniPrimary (citable) accession number: Q9D6Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.