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Protein

Solute carrier family 52, riboflavin transporter, member 3

Gene

Slc52a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transporter for riboflavin, which must be obtained as a nutrient via intestinal absorption. Riboflavin transport is Na+-independent at low pH but significantly reduced by Na+ depletion under neutral pH conditions. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), by methylene blue, and to a lesser extent by amiloride (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to heat Source: MGI
  • riboflavin transport Source: UniProtKB
  • sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_324263. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 52, riboflavin transporter, member 3
Alternative name(s):
Riboflavin transporter 2
Short name:
RFT2
Gene namesi
Name:Slc52a3
Synonyms:Rft2, RFVT3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916948. Slc52a3.

Subcellular locationi

  • Apical cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721HelicalSequence AnalysisAdd
BLAST
Topological domaini28 – 3710ExtracellularSequence Analysis
Transmembranei38 – 5821HelicalSequence AnalysisAdd
BLAST
Topological domaini59 – 7113CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence AnalysisAdd
BLAST
Topological domaini93 – 10513ExtracellularSequence AnalysisAdd
BLAST
Transmembranei106 – 12621HelicalSequence AnalysisAdd
BLAST
Topological domaini127 – 13711CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei138 – 15821HelicalSequence AnalysisAdd
BLAST
Topological domaini159 – 21153ExtracellularSequence AnalysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence AnalysisAdd
BLAST
Topological domaini233 – 29159CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence AnalysisAdd
BLAST
Topological domaini313 – 32614ExtracellularSequence AnalysisAdd
BLAST
Transmembranei327 – 34721HelicalSequence AnalysisAdd
BLAST
Topological domaini348 – 3503CytoplasmicSequence Analysis
Transmembranei351 – 37121HelicalSequence AnalysisAdd
BLAST
Topological domaini372 – 38716ExtracellularSequence AnalysisAdd
BLAST
Transmembranei388 – 40821HelicalSequence AnalysisAdd
BLAST
Topological domaini409 – 41810CytoplasmicSequence Analysis
Transmembranei419 – 43921HelicalSequence AnalysisAdd
BLAST
Topological domaini440 – 46021ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Solute carrier family 52, riboflavin transporter, member 3PRO_0000042637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi377 ↔ 454By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9D6X5.
PRIDEiQ9D6X5.

PTM databases

PhosphoSiteiQ9D6X5.

Expressioni

Tissue specificityi

Within the small intestine, it is particulary expressed in the jujenum and the ileum. Almost negligible expression in the stomach, duodenum, and large intestine.1 Publication

Gene expression databases

BgeeiQ9D6X5.
CleanExiMM_2310046K01RIK.
GenevisibleiQ9D6X5. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9D6X5. 1 interaction.
MINTiMINT-218754.
STRINGi10090.ENSMUSP00000072961.

Structurei

3D structure databases

ProteinModelPortaliQ9D6X5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the riboflavin transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG326568.
GeneTreeiENSGT00390000003774.
HOGENOMiHOG000247012.
HOVERGENiHBG051170.
InParanoidiQ9D6X5.
KOiK14620.
OMAiMELPEGW.
OrthoDBiEOG7XDBFX.
PhylomeDBiQ9D6X5.
TreeFamiTF314820.

Family and domain databases

InterProiIPR009357. Endogenous_retrovirus_rcpt.
[Graphical view]
PANTHERiPTHR12929. PTHR12929. 1 hit.
PfamiPF06237. DUF1011. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6X5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFLTHLLVC VFGMGSWVAI NGLWVELPLL VTELPEAWYL PSYLTVVIQL
60 70 80 90 100
ANIGPLLVTL MHRFRPGCLS EVPVIFLILC VGTAACILLA FLWNVTSWIQ
110 120 130 140 150
GGQHSVAFIV LTFFLALVDC TSSVTFLPFM SQLPTYYLTT FFIGEGLSGL
160 170 180 190 200
LPALVALVQG SGITTCVNVT ETPGTTLNTM ETPITQGNLS PSLPSPSWHQ
210 220 230 240 250
ESRYLAPRFS PLLFFLLLSF LTGCCLVAFF LLQRQPWGRQ GSIEDLLHSQ
260 270 280 290 300
VTLHSIRPRD TEDTSSLGAP VSSPGKGSVE ASVASLRPAQ LAFIYSVVAF
310 320 330 340 350
VNALTNGVLP SVQTYSCLPY GPVAYHLSAT LSSVASPLAC FLPIFLPNRS
360 370 380 390 400
LLFLGVLTVL GTGFGAYNMA MAAMSPCPVL QGHWGGEVLI VLSWVLFAAC
410 420 430 440 450
LSYVKVMLGV ILRDRSRSAL LWCGAAVQLG SLIGALLMFP LVNVLKLFSS
460
ADYCSLDCSV
Length:460
Mass (Da):49,559
Last modified:October 5, 2010 - v3
Checksum:i7F299163AD3C884E
GO
Isoform 2 (identifier: Q9D6X5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     187-250: GNLSPSLPSP...GSIEDLLHSQ → VAVIPGGAHS...CQGDAGCDLA
     251-460: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):26,770
Checksum:iA10C6C43BBEF31C4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811V → L in BAE41605 (PubMed:16141072).Curated
Sequence conflicti182 – 1821T → N in BAB26542 (PubMed:16141072).Curated
Sequence conflicti195 – 1951S → P in BAE41605 (PubMed:16141072).Curated
Sequence conflicti223 – 2231G → C in BAB26542 (PubMed:16141072).Curated
Sequence conflicti286 – 2861L → I in BAE41605 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei187 – 25064GNLSP…LLHSQ → VAVIPGGAHSVGDRLWGLQY GHGCYEPLPCPAGSLGWRSP YRALLGAVCSLSQLCQGDAG CDLA in isoform 2. 1 PublicationVSP_003816Add
BLAST
Alternative sequencei251 – 460210Missing in isoform 2. 1 PublicationVSP_003817Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009850 mRNA. Translation: BAB26542.1.
AK029091 mRNA. Translation: BAC26290.1.
AK154970 mRNA. Translation: BAE32962.1.
AK170158 mRNA. Translation: BAE41605.1.
AL845161 Genomic DNA. Translation: CAM17705.1.
AL845161 Genomic DNA. Translation: CAM17704.1.
CH466551 Genomic DNA. Translation: EDL05945.1.
BC016127 mRNA. Translation: AAH16127.1.
CCDSiCCDS16876.1. [Q9D6X5-1]
CCDS50749.1. [Q9D6X5-2]
RefSeqiNP_001158291.1. NM_001164819.1. [Q9D6X5-1]
NP_001158292.1. NM_001164820.1. [Q9D6X5-2]
NP_081448.2. NM_027172.3. [Q9D6X5-1]
UniGeneiMm.258262.

Genome annotation databases

EnsembliENSMUST00000073228; ENSMUSP00000072961; ENSMUSG00000027463. [Q9D6X5-1]
ENSMUST00000109858; ENSMUSP00000105484; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109859; ENSMUSP00000105485; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109861; ENSMUSP00000105487; ENSMUSG00000027463. [Q9D6X5-1]
GeneIDi69698.
KEGGimmu:69698.
UCSCiuc008neu.2. mouse. [Q9D6X5-1]
uc012cga.1. mouse. [Q9D6X5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009850 mRNA. Translation: BAB26542.1.
AK029091 mRNA. Translation: BAC26290.1.
AK154970 mRNA. Translation: BAE32962.1.
AK170158 mRNA. Translation: BAE41605.1.
AL845161 Genomic DNA. Translation: CAM17705.1.
AL845161 Genomic DNA. Translation: CAM17704.1.
CH466551 Genomic DNA. Translation: EDL05945.1.
BC016127 mRNA. Translation: AAH16127.1.
CCDSiCCDS16876.1. [Q9D6X5-1]
CCDS50749.1. [Q9D6X5-2]
RefSeqiNP_001158291.1. NM_001164819.1. [Q9D6X5-1]
NP_001158292.1. NM_001164820.1. [Q9D6X5-2]
NP_081448.2. NM_027172.3. [Q9D6X5-1]
UniGeneiMm.258262.

3D structure databases

ProteinModelPortaliQ9D6X5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D6X5. 1 interaction.
MINTiMINT-218754.
STRINGi10090.ENSMUSP00000072961.

PTM databases

PhosphoSiteiQ9D6X5.

Proteomic databases

PaxDbiQ9D6X5.
PRIDEiQ9D6X5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073228; ENSMUSP00000072961; ENSMUSG00000027463. [Q9D6X5-1]
ENSMUST00000109858; ENSMUSP00000105484; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109859; ENSMUSP00000105485; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109861; ENSMUSP00000105487; ENSMUSG00000027463. [Q9D6X5-1]
GeneIDi69698.
KEGGimmu:69698.
UCSCiuc008neu.2. mouse. [Q9D6X5-1]
uc012cga.1. mouse. [Q9D6X5-2]

Organism-specific databases

CTDi113278.
MGIiMGI:1916948. Slc52a3.

Phylogenomic databases

eggNOGiNOG326568.
GeneTreeiENSGT00390000003774.
HOGENOMiHOG000247012.
HOVERGENiHBG051170.
InParanoidiQ9D6X5.
KOiK14620.
OMAiMELPEGW.
OrthoDBiEOG7XDBFX.
PhylomeDBiQ9D6X5.
TreeFamiTF314820.

Enzyme and pathway databases

ReactomeiREACT_324263. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

NextBioi330112.
PROiQ9D6X5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6X5.
CleanExiMM_2310046K01RIK.
GenevisibleiQ9D6X5. MM.

Family and domain databases

InterProiIPR009357. Endogenous_retrovirus_rcpt.
[Graphical view]
PANTHERiPTHR12929. PTHR12929. 1 hit.
PfamiPF06237. DUF1011. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Skin and Tongue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Salivary gland.
  5. "Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption."
    Yoshimatsu H., Yonezawa A., Yao Y., Sugano K., Nakagawa S., Omura T., Matsubara K.
    Am. J. Physiol. 306:G102-G110(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiS52A3_MOUSE
AccessioniPrimary (citable) accession number: Q9D6X5
Secondary accession number(s): A2AQU3
, Q3TDJ6, Q3U328, Q8CE36, Q91WB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: October 5, 2010
Last modified: July 22, 2015
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.