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Protein

Solute carrier family 52, riboflavin transporter, member 3

Gene

Slc52a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transporter for riboflavin, which must be obtained as a nutrient via intestinal absorption. Riboflavin transport is Na+-independent at low pH but significantly reduced by Na+ depletion under neutral pH conditions. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), by methylene blue, and to a lesser extent by amiloride (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to heat Source: MGI
  • riboflavin transport Source: UniProtKB
  • sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-196843. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 52, riboflavin transporter, member 3
Alternative name(s):
Riboflavin transporter 2
Short name:
RFT2
Gene namesi
Name:Slc52a3
Synonyms:Rft2, RFVT3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916948. Slc52a3.

Subcellular locationi

  • Apical cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 37ExtracellularSequence analysis10
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 71CytoplasmicSequence analysisAdd BLAST13
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Topological domaini93 – 105ExtracellularSequence analysisAdd BLAST13
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Topological domaini127 – 137CytoplasmicSequence analysisAdd BLAST11
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Topological domaini159 – 211ExtracellularSequence analysisAdd BLAST53
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 291CytoplasmicSequence analysisAdd BLAST59
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 326ExtracellularSequence analysisAdd BLAST14
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Topological domaini348 – 350CytoplasmicSequence analysis3
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Topological domaini372 – 387ExtracellularSequence analysisAdd BLAST16
Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
Topological domaini409 – 418CytoplasmicSequence analysis10
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21
Topological domaini440 – 460ExtracellularSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000426371 – 460Solute carrier family 52, riboflavin transporter, member 3Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Modified residuei242PhosphoserineBy similarity1
Modified residuei266PhosphoserineCombined sources1
Disulfide bondi377 ↔ 454By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9D6X5.
PeptideAtlasiQ9D6X5.
PRIDEiQ9D6X5.

PTM databases

iPTMnetiQ9D6X5.
PhosphoSitePlusiQ9D6X5.

Expressioni

Tissue specificityi

Within the small intestine, it is particulary expressed in the jujenum and the ileum. Almost negligible expression in the stomach, duodenum, and large intestine.1 Publication

Gene expression databases

BgeeiENSMUSG00000027463.
CleanExiMM_2310046K01RIK.
GenevisibleiQ9D6X5. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9D6X5. 1 interactor.
MINTiMINT-218754.
STRINGi10090.ENSMUSP00000072961.

Structurei

3D structure databases

ProteinModelPortaliQ9D6X5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the riboflavin transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4255. Eukaryota.
ENOG410YE1U. LUCA.
GeneTreeiENSGT00390000003774.
HOGENOMiHOG000247012.
HOVERGENiHBG051170.
InParanoidiQ9D6X5.
KOiK14620.
OMAiAMFLHFT.
OrthoDBiEOG091G0BZA.
PhylomeDBiQ9D6X5.
TreeFamiTF314820.

Family and domain databases

InterProiIPR009357. Riboflavin_transptr.
[Graphical view]
PANTHERiPTHR12929. PTHR12929. 1 hit.
PfamiPF06237. DUF1011. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6X5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFLTHLLVC VFGMGSWVAI NGLWVELPLL VTELPEAWYL PSYLTVVIQL
60 70 80 90 100
ANIGPLLVTL MHRFRPGCLS EVPVIFLILC VGTAACILLA FLWNVTSWIQ
110 120 130 140 150
GGQHSVAFIV LTFFLALVDC TSSVTFLPFM SQLPTYYLTT FFIGEGLSGL
160 170 180 190 200
LPALVALVQG SGITTCVNVT ETPGTTLNTM ETPITQGNLS PSLPSPSWHQ
210 220 230 240 250
ESRYLAPRFS PLLFFLLLSF LTGCCLVAFF LLQRQPWGRQ GSIEDLLHSQ
260 270 280 290 300
VTLHSIRPRD TEDTSSLGAP VSSPGKGSVE ASVASLRPAQ LAFIYSVVAF
310 320 330 340 350
VNALTNGVLP SVQTYSCLPY GPVAYHLSAT LSSVASPLAC FLPIFLPNRS
360 370 380 390 400
LLFLGVLTVL GTGFGAYNMA MAAMSPCPVL QGHWGGEVLI VLSWVLFAAC
410 420 430 440 450
LSYVKVMLGV ILRDRSRSAL LWCGAAVQLG SLIGALLMFP LVNVLKLFSS
460
ADYCSLDCSV
Length:460
Mass (Da):49,559
Last modified:October 5, 2010 - v3
Checksum:i7F299163AD3C884E
GO
Isoform 2 (identifier: Q9D6X5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     187-250: GNLSPSLPSP...GSIEDLLHSQ → VAVIPGGAHS...CQGDAGCDLA
     251-460: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):26,770
Checksum:iA10C6C43BBEF31C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81V → L in BAE41605 (PubMed:16141072).Curated1
Sequence conflicti182T → N in BAB26542 (PubMed:16141072).Curated1
Sequence conflicti195S → P in BAE41605 (PubMed:16141072).Curated1
Sequence conflicti223G → C in BAB26542 (PubMed:16141072).Curated1
Sequence conflicti286L → I in BAE41605 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003816187 – 250GNLSP…LLHSQ → VAVIPGGAHSVGDRLWGLQY GHGCYEPLPCPAGSLGWRSP YRALLGAVCSLSQLCQGDAG CDLA in isoform 2. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_003817251 – 460Missing in isoform 2. 1 PublicationAdd BLAST210

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009850 mRNA. Translation: BAB26542.1.
AK029091 mRNA. Translation: BAC26290.1.
AK154970 mRNA. Translation: BAE32962.1.
AK170158 mRNA. Translation: BAE41605.1.
AL845161 Genomic DNA. Translation: CAM17705.1.
AL845161 Genomic DNA. Translation: CAM17704.1.
CH466551 Genomic DNA. Translation: EDL05945.1.
BC016127 mRNA. Translation: AAH16127.1.
CCDSiCCDS16876.1. [Q9D6X5-1]
CCDS50749.1. [Q9D6X5-2]
RefSeqiNP_001158291.1. NM_001164819.1. [Q9D6X5-1]
NP_001158292.1. NM_001164820.1. [Q9D6X5-2]
NP_081448.2. NM_027172.3. [Q9D6X5-1]
UniGeneiMm.258262.

Genome annotation databases

EnsembliENSMUST00000073228; ENSMUSP00000072961; ENSMUSG00000027463. [Q9D6X5-1]
ENSMUST00000109858; ENSMUSP00000105484; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109859; ENSMUSP00000105485; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109861; ENSMUSP00000105487; ENSMUSG00000027463. [Q9D6X5-1]
GeneIDi69698.
KEGGimmu:69698.
UCSCiuc008neu.2. mouse. [Q9D6X5-1]
uc012cga.1. mouse. [Q9D6X5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009850 mRNA. Translation: BAB26542.1.
AK029091 mRNA. Translation: BAC26290.1.
AK154970 mRNA. Translation: BAE32962.1.
AK170158 mRNA. Translation: BAE41605.1.
AL845161 Genomic DNA. Translation: CAM17705.1.
AL845161 Genomic DNA. Translation: CAM17704.1.
CH466551 Genomic DNA. Translation: EDL05945.1.
BC016127 mRNA. Translation: AAH16127.1.
CCDSiCCDS16876.1. [Q9D6X5-1]
CCDS50749.1. [Q9D6X5-2]
RefSeqiNP_001158291.1. NM_001164819.1. [Q9D6X5-1]
NP_001158292.1. NM_001164820.1. [Q9D6X5-2]
NP_081448.2. NM_027172.3. [Q9D6X5-1]
UniGeneiMm.258262.

3D structure databases

ProteinModelPortaliQ9D6X5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D6X5. 1 interactor.
MINTiMINT-218754.
STRINGi10090.ENSMUSP00000072961.

PTM databases

iPTMnetiQ9D6X5.
PhosphoSitePlusiQ9D6X5.

Proteomic databases

PaxDbiQ9D6X5.
PeptideAtlasiQ9D6X5.
PRIDEiQ9D6X5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073228; ENSMUSP00000072961; ENSMUSG00000027463. [Q9D6X5-1]
ENSMUST00000109858; ENSMUSP00000105484; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109859; ENSMUSP00000105485; ENSMUSG00000027463. [Q9D6X5-2]
ENSMUST00000109861; ENSMUSP00000105487; ENSMUSG00000027463. [Q9D6X5-1]
GeneIDi69698.
KEGGimmu:69698.
UCSCiuc008neu.2. mouse. [Q9D6X5-1]
uc012cga.1. mouse. [Q9D6X5-2]

Organism-specific databases

CTDi113278.
MGIiMGI:1916948. Slc52a3.

Phylogenomic databases

eggNOGiKOG4255. Eukaryota.
ENOG410YE1U. LUCA.
GeneTreeiENSGT00390000003774.
HOGENOMiHOG000247012.
HOVERGENiHBG051170.
InParanoidiQ9D6X5.
KOiK14620.
OMAiAMFLHFT.
OrthoDBiEOG091G0BZA.
PhylomeDBiQ9D6X5.
TreeFamiTF314820.

Enzyme and pathway databases

ReactomeiR-MMU-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ9D6X5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027463.
CleanExiMM_2310046K01RIK.
GenevisibleiQ9D6X5. MM.

Family and domain databases

InterProiIPR009357. Riboflavin_transptr.
[Graphical view]
PANTHERiPTHR12929. PTHR12929. 1 hit.
PfamiPF06237. DUF1011. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS52A3_MOUSE
AccessioniPrimary (citable) accession number: Q9D6X5
Secondary accession number(s): A2AQU3
, Q3TDJ6, Q3U328, Q8CE36, Q91WB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.