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Protein

Nitric oxide synthase-interacting protein

Gene

Nosip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (PubMed:25546391). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-203754 NOSIP mediated eNOS trafficking
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-interacting protein
Alternative name(s):
E3 ubiquitin-protein ligase NOSIP1 Publication (EC:2.3.2.27)
RING-type E3 ubiquitin transferase NOSIPCurated
Gene namesi
Name:Nosip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913644 Nosip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Although mutant embryos are present at the expected Mendelian rate at 18.5 dpc, they die shortly after birth with signs of respiratory distress and cyanosis, likely due to craniofacial malformations. Malformations in knockout mice range from ocular hypotelorism, narrow snout, laterally cleft lip, and cleft secondary palate to cyclopia and presence of proboscis or a single head-like protrusion devoid of facial features. In addition, the weight of knockout embryos is significantly reduced. In knockout animals, PP2A activity is increased in palatal and facial tissues as compared to wild-type, but not in lungs, which do not seem to be affected by the mutation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805871 – 301Nitric oxide synthase-interacting proteinAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D6T0
PaxDbiQ9D6T0
PeptideAtlasiQ9D6T0
PRIDEiQ9D6T0

PTM databases

iPTMnetiQ9D6T0
PhosphoSitePlusiQ9D6T0

Expressioni

Gene expression databases

BgeeiENSMUSG00000003421
CleanExiMM_NOSIP
ExpressionAtlasiQ9D6T0 baseline and differential
GenevisibleiQ9D6T0 MM

Interactioni

Subunit structurei

Interacts with NOS1 and NOS3 (By similarity). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (PubMed:25546391).By similarity1 Publication

Protein-protein interaction databases

BioGridi211438, 2 interactors
IntActiQ9D6T0, 1 interactor
STRINGi10090.ENSMUSP00000003513

Structurei

3D structure databases

ProteinModelPortaliQ9D6T0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 75U-box-likeAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 101Nuclear localization signalBy similarityAdd BLAST24

Domaini

The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.

Sequence similaritiesi

Belongs to the NOSIP family.Curated

Phylogenomic databases

eggNOGiKOG3039 Eukaryota
ENOG410XQYR LUCA
GeneTreeiENSGT00390000015505
HOGENOMiHOG000007498
HOVERGENiHBG082046
InParanoidiQ9D6T0
KOiK13125
OMAiIFCRECA
OrthoDBiEOG091G0EHZ
PhylomeDBiQ9D6T0
TreeFamiTF314268

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR016818 NOSIP
IPR031790 Znf-NOSIP
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF15906 zf-NOSIP, 1 hit
PIRSFiPIRSF023577 ENOS_interacting, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL
60 70 80 90 100
QPCHDPVVTP DGYLYEREAI LEYILHQKRE IARQVKAYEK QRGARREEQK
110 120 130 140 150
ELQRAAAQDQ VRGFLEKEAA IVSRPLNPFM PKAATLPNTE GEQPGPSVGP
160 170 180 190 200
VGKDKDKALP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTSV
210 220 230 240 250
RFTQLDDSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC
260 270 280 290 300
VEKLIRKDMV DPVNGDTLTE RDIIVLQRGG TGFAGSGVKL QAEMSRPVMQ

A
Length:301
Mass (Da):33,209
Last modified:June 1, 2001 - v1
Checksum:i9953A7F6E6B4A5FF
GO
Isoform 2 (identifier: Q9D6T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     180-204: Missing.

Note: No experimental confirmation available.
Show »
Length:276
Mass (Da):30,428
Checksum:iED57643C3EABF1A9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023795180 – 204Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007962 mRNA Translation: BAB25373.1
AK010006 mRNA Translation: BAB26637.1
BC089029 mRNA Translation: AAH89029.1
CCDSiCCDS21228.1 [Q9D6T0-1]
CCDS52242.1 [Q9D6T0-2]
RefSeqiNP_001157156.1, NM_001163684.1 [Q9D6T0-2]
NP_079809.1, NM_025533.3 [Q9D6T0-1]
XP_017167721.1, XM_017312232.1 [Q9D6T0-1]
UniGeneiMm.272139

Genome annotation databases

EnsembliENSMUST00000003513; ENSMUSP00000003513; ENSMUSG00000003421 [Q9D6T0-1]
ENSMUST00000107829; ENSMUSP00000103460; ENSMUSG00000003421 [Q9D6T0-2]
GeneIDi66394
KEGGimmu:66394
UCSCiuc009gsx.2 mouse [Q9D6T0-1]
uc009gsy.2 mouse [Q9D6T0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNOSIP_MOUSE
AccessioniPrimary (citable) accession number: Q9D6T0
Secondary accession number(s): Q9D8J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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