Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitric oxide synthase-interacting protein

Gene

Nosip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (PubMed:25546391). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-203754. NOSIP mediated eNOS trafficking.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-interacting protein
Alternative name(s):
E3 ubiquitin-protein ligase NOSIP1 Publication (EC:6.3.2.-)
Gene namesi
Name:Nosip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913644. Nosip.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Although mutant embryos are present at the expected Mendelian rate at 18.5 dpc, they die shortly after birth with signs of respiratory distress and cyanosis, likely due to craniofacial malformations. Malformations in knockout mice range from ocular hypotelorism, narrow snout, laterally cleft lip, and cleft secondary palate to cyclopia and presence of proboscis or a single head-like protrusion devoid of facial features. In addition, the weight of knockout embryos is significantly reduced. In knockout animals, PP2A activity is increased in palatal and facial tissues as compared to wild-type, but not in lungs, which do not seem to be affected by the mutation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805871 – 301Nitric oxide synthase-interacting proteinAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D6T0.
PaxDbiQ9D6T0.
PeptideAtlasiQ9D6T0.
PRIDEiQ9D6T0.

PTM databases

iPTMnetiQ9D6T0.
PhosphoSitePlusiQ9D6T0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003421.
CleanExiMM_NOSIP.
GenevisibleiQ9D6T0. MM.

Interactioni

Subunit structurei

Interacts with NOS1 and NOS3 (By similarity). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (PubMed:25546391).By similarity1 Publication

Protein-protein interaction databases

BioGridi211438. 1 interactor.
STRINGi10090.ENSMUSP00000003513.

Structurei

3D structure databases

ProteinModelPortaliQ9D6T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 75U-box-likeAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 101Nuclear localization signalBy similarityAdd BLAST24

Domaini

The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.

Sequence similaritiesi

Belongs to the NOSIP family.Curated

Phylogenomic databases

eggNOGiKOG3039. Eukaryota.
ENOG410XQYR. LUCA.
GeneTreeiENSGT00390000015505.
HOGENOMiHOG000007498.
HOVERGENiHBG082046.
InParanoidiQ9D6T0.
KOiK13125.
OMAiPDGFLYE.
OrthoDBiEOG091G0EHZ.
PhylomeDBiQ9D6T0.
TreeFamiTF314268.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR016818. Nitric_oxide_synth-interacting.
IPR031790. Znf-NOSIP.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13063:SF10. PTHR13063:SF10. 1 hit.
PfamiPF15906. zf-NOSIP. 1 hit.
[Graphical view]
PIRSFiPIRSF023577. ENOS_interacting. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL
60 70 80 90 100
QPCHDPVVTP DGYLYEREAI LEYILHQKRE IARQVKAYEK QRGARREEQK
110 120 130 140 150
ELQRAAAQDQ VRGFLEKEAA IVSRPLNPFM PKAATLPNTE GEQPGPSVGP
160 170 180 190 200
VGKDKDKALP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTSV
210 220 230 240 250
RFTQLDDSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC
260 270 280 290 300
VEKLIRKDMV DPVNGDTLTE RDIIVLQRGG TGFAGSGVKL QAEMSRPVMQ

A
Length:301
Mass (Da):33,209
Last modified:June 1, 2001 - v1
Checksum:i9953A7F6E6B4A5FF
GO
Isoform 2 (identifier: Q9D6T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     180-204: Missing.

Note: No experimental confirmation available.
Show »
Length:276
Mass (Da):30,428
Checksum:iED57643C3EABF1A9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023795180 – 204Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007962 mRNA. Translation: BAB25373.1.
AK010006 mRNA. Translation: BAB26637.1.
BC089029 mRNA. Translation: AAH89029.1.
CCDSiCCDS21228.1. [Q9D6T0-1]
CCDS52242.1. [Q9D6T0-2]
RefSeqiNP_001157156.1. NM_001163684.1. [Q9D6T0-2]
NP_079809.1. NM_025533.3. [Q9D6T0-1]
XP_017167721.1. XM_017312232.1. [Q9D6T0-1]
UniGeneiMm.272139.

Genome annotation databases

EnsembliENSMUST00000003513; ENSMUSP00000003513; ENSMUSG00000003421. [Q9D6T0-1]
ENSMUST00000107829; ENSMUSP00000103460; ENSMUSG00000003421. [Q9D6T0-2]
GeneIDi66394.
KEGGimmu:66394.
UCSCiuc009gsx.2. mouse. [Q9D6T0-1]
uc009gsy.2. mouse. [Q9D6T0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007962 mRNA. Translation: BAB25373.1.
AK010006 mRNA. Translation: BAB26637.1.
BC089029 mRNA. Translation: AAH89029.1.
CCDSiCCDS21228.1. [Q9D6T0-1]
CCDS52242.1. [Q9D6T0-2]
RefSeqiNP_001157156.1. NM_001163684.1. [Q9D6T0-2]
NP_079809.1. NM_025533.3. [Q9D6T0-1]
XP_017167721.1. XM_017312232.1. [Q9D6T0-1]
UniGeneiMm.272139.

3D structure databases

ProteinModelPortaliQ9D6T0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211438. 1 interactor.
STRINGi10090.ENSMUSP00000003513.

PTM databases

iPTMnetiQ9D6T0.
PhosphoSitePlusiQ9D6T0.

Proteomic databases

EPDiQ9D6T0.
PaxDbiQ9D6T0.
PeptideAtlasiQ9D6T0.
PRIDEiQ9D6T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003513; ENSMUSP00000003513; ENSMUSG00000003421. [Q9D6T0-1]
ENSMUST00000107829; ENSMUSP00000103460; ENSMUSG00000003421. [Q9D6T0-2]
GeneIDi66394.
KEGGimmu:66394.
UCSCiuc009gsx.2. mouse. [Q9D6T0-1]
uc009gsy.2. mouse. [Q9D6T0-2]

Organism-specific databases

CTDi51070.
MGIiMGI:1913644. Nosip.

Phylogenomic databases

eggNOGiKOG3039. Eukaryota.
ENOG410XQYR. LUCA.
GeneTreeiENSGT00390000015505.
HOGENOMiHOG000007498.
HOVERGENiHBG082046.
InParanoidiQ9D6T0.
KOiK13125.
OMAiPDGFLYE.
OrthoDBiEOG091G0EHZ.
PhylomeDBiQ9D6T0.
TreeFamiTF314268.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-203754. NOSIP mediated eNOS trafficking.

Miscellaneous databases

ChiTaRSiNosip. mouse.
PROiQ9D6T0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003421.
CleanExiMM_NOSIP.
GenevisibleiQ9D6T0. MM.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR016818. Nitric_oxide_synth-interacting.
IPR031790. Znf-NOSIP.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13063:SF10. PTHR13063:SF10. 1 hit.
PfamiPF15906. zf-NOSIP. 1 hit.
[Graphical view]
PIRSFiPIRSF023577. ENOS_interacting. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOSIP_MOUSE
AccessioniPrimary (citable) accession number: Q9D6T0
Secondary accession number(s): Q9D8J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.