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Q9D6R2

- IDH3A_MOUSE

UniProt

Q9D6R2 - IDH3A_MOUSE

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Protein
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
Gene
Idh3a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151Substrate By similarity
Binding sitei125 – 1251Substrate By similarity
Binding sitei146 – 1461Substrate By similarity
Sitei153 – 1531Critical for catalysis By similarity
Sitei200 – 2001Critical for catalysis By similarity
Metal bindingi233 – 2331Magnesium or manganese By similarity
Binding sitei233 – 2331Substrate By similarity
Metal bindingi257 – 2571Magnesium or manganese By similarity
Metal bindingi261 – 2611Magnesium or manganese By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene namesi
Name:Idh3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1915084. Idh3a.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727Mitochondrion By similarity
Add
BLAST
Chaini28 – 366339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
PRO_0000014438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771N6-succinyllysine1 Publication
Modified residuei223 – 2231N6-acetyllysine1 Publication
Modified residuei343 – 3431N6-acetyllysine; alternate By similarity
Modified residuei343 – 3431N6-succinyllysine; alternate1 Publication
Modified residuei350 – 3501N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9D6R2.
PaxDbiQ9D6R2.
PRIDEiQ9D6R2.

2D gel databases

REPRODUCTION-2DPAGEQ9D6R2.
UCD-2DPAGEQ9D6R2.

PTM databases

PhosphoSiteiQ9D6R2.

Expressioni

Gene expression databases

BgeeiQ9D6R2.
CleanExiMM_IDH3A.
GenevestigatoriQ9D6R2.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.

Protein-protein interaction databases

IntActiQ9D6R2. 8 interactions.
MINTiMINT-1861233.

Structurei

3D structure databases

ProteinModelPortaliQ9D6R2.
SMRiQ9D6R2. Positions 30-364.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00550000074918.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ9D6R2.
KOiK00030.
OMAiTPIAWEP.
OrthoDBiEOG75B85R.
PhylomeDBiQ9D6R2.
TreeFamiTF105692.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9D6R2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV    50
MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK 100
TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE 150
GEYSGIEHVI VDGVVQSIKL ITEEASKRIA EFAFEYARNN HRSNVTAVHK 200
ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV QDPSQFDVLV 250
MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD 300
MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNAK 350
CSDFTEEICR RVKDLD 366
Length:366
Mass (Da):39,639
Last modified:June 1, 2001 - v1
Checksum:i9F1D68C269376955
GO
Isoform 2 (identifier: Q9D6R2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:288
Mass (Da):31,462
Checksum:i5A6F91BF1BBD4FC5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7878Missing in isoform 2.
VSP_014517Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 3061A → T in BAC33199. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK003393 mRNA. Translation: BAB22760.1.
AK010065 mRNA. Translation: BAB26679.1.
AK032787 mRNA. Translation: BAC28021.1.
AK047951 mRNA. Translation: BAC33199.1.
AK150618 mRNA. Translation: BAE29708.1.
AK151304 mRNA. Translation: BAE30286.1.
AK152353 mRNA. Translation: BAE31145.1.
AK153459 mRNA. Translation: BAE32011.1.
AK158646 mRNA. Translation: BAE34596.1.
AK159051 mRNA. Translation: BAE34785.1.
AK168049 mRNA. Translation: BAE40031.1.
AK168149 mRNA. Translation: BAE40114.1.
AK169152 mRNA. Translation: BAE40931.1.
BC034273 mRNA. Translation: AAH34273.1.
BC049956 mRNA. Translation: AAH49956.1.
CCDSiCCDS23191.1. [Q9D6R2-1]
RefSeqiNP_083849.1. NM_029573.2. [Q9D6R2-1]
UniGeneiMm.279195.

Genome annotation databases

EnsembliENSMUST00000167866; ENSMUSP00000127526; ENSMUSG00000032279. [Q9D6R2-1]
GeneIDi67834.
KEGGimmu:67834.
UCSCiuc009prg.1. mouse. [Q9D6R2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK003393 mRNA. Translation: BAB22760.1 .
AK010065 mRNA. Translation: BAB26679.1 .
AK032787 mRNA. Translation: BAC28021.1 .
AK047951 mRNA. Translation: BAC33199.1 .
AK150618 mRNA. Translation: BAE29708.1 .
AK151304 mRNA. Translation: BAE30286.1 .
AK152353 mRNA. Translation: BAE31145.1 .
AK153459 mRNA. Translation: BAE32011.1 .
AK158646 mRNA. Translation: BAE34596.1 .
AK159051 mRNA. Translation: BAE34785.1 .
AK168049 mRNA. Translation: BAE40031.1 .
AK168149 mRNA. Translation: BAE40114.1 .
AK169152 mRNA. Translation: BAE40931.1 .
BC034273 mRNA. Translation: AAH34273.1 .
BC049956 mRNA. Translation: AAH49956.1 .
CCDSi CCDS23191.1. [Q9D6R2-1 ]
RefSeqi NP_083849.1. NM_029573.2. [Q9D6R2-1 ]
UniGenei Mm.279195.

3D structure databases

ProteinModelPortali Q9D6R2.
SMRi Q9D6R2. Positions 30-364.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9D6R2. 8 interactions.
MINTi MINT-1861233.

PTM databases

PhosphoSitei Q9D6R2.

2D gel databases

REPRODUCTION-2DPAGE Q9D6R2.
UCD-2DPAGE Q9D6R2.

Proteomic databases

MaxQBi Q9D6R2.
PaxDbi Q9D6R2.
PRIDEi Q9D6R2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000167866 ; ENSMUSP00000127526 ; ENSMUSG00000032279 . [Q9D6R2-1 ]
GeneIDi 67834.
KEGGi mmu:67834.
UCSCi uc009prg.1. mouse. [Q9D6R2-1 ]

Organism-specific databases

CTDi 3419.
MGIi MGI:1915084. Idh3a.

Phylogenomic databases

eggNOGi COG0473.
GeneTreei ENSGT00550000074918.
HOGENOMi HOG000021113.
HOVERGENi HBG052080.
InParanoidi Q9D6R2.
KOi K00030.
OMAi TPIAWEP.
OrthoDBi EOG75B85R.
PhylomeDBi Q9D6R2.
TreeFami TF105692.

Miscellaneous databases

NextBioi 325657.
PROi Q9D6R2.
SOURCEi Search...

Gene expression databases

Bgeei Q9D6R2.
CleanExi MM_IDH3A.
Genevestigatori Q9D6R2.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and DBA/2.
    Tissue: Bone marrow, Heart, Tongue, Visual cortex and Wolffian duct.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Liver.
  3. Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 59-85; 101-188; 206-214 AND 300-336, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-77; LYS-343 AND LYS-350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIDH3A_MOUSE
AccessioniPrimary (citable) accession number: Q9D6R2
Secondary accession number(s): Q3UAM8, Q8C8A1, Q9D1L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi