Reviewed, UniProtKB/Swiss-Prot Q9D6R2 (IDH3A_MOUSE)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

Idh3a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D6R2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9D6R2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 366	339	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014438
Sites
	Metal binding	233	1	Magnesium or manganese By similarity
	Metal binding	257	1	Magnesium or manganese By similarity
	Metal binding	261	1	Magnesium or manganese By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity
	Site	153	1	Critical for catalysis By similarity
	Site	200	1	Critical for catalysis By similarity
Natural variations
	Alternative sequence	1 – 78	78	Missing in isoform 2.		VSP_014517
Experimental info
	Sequence conflict	306	1	A → T in BAC33199. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9F1D68C269376955	FASTA	366	39,639
	10 20 30 40 50 60 MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV MKIFDAAKAP 70 80 90 100 110 120 IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK TPIAAGHPSM NLLLRKTFDL 130 140 150 160 170 180 YANVRPCVSI EGYKTPYTDV NIVTIRENTE GEYSGIEHVI VDGVVQSIKL ITEEASKRIA 190 200 210 220 230 240 EFAFEYARNN HRSNVTAVHK ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV 250 260 270 280 290 300 QDPSQFDVLV MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD 310 320 330 340 350 360 MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNAK CSDFTEEICR RVKDLD
	Isoform 2. Checksum: 5A6F91BF1BBD4FC5	FASTA	288	31,462
	10 20 30 40 50 60 MIPPEAKESM DKNKMGLKGP LKTPIAAGHP SMNLLLRKTF DLYANVRPCV SIEGYKTPYT 70 80 90 100 110 120 DVNIVTIREN TEGEYSGIEH VIVDGVVQSI KLITEEASKR IAEFAFEYAR NNHRSNVTAV 130 140 150 160 170 180 HKANIMRMSD GLFLQKCREV AENCKDIKFN EMYLDTVCLN MVQDPSQFDV LVMPNLYGDI 190 200 210 220 230 240 LSDLCAGLIG GLGVTPSGNI GANGVAIFES VHGTAPDIAG KDMANPTALL LSAVMMLRHM 250 260 270 280 GLFDHAAKIE AACFATIKDG KSLTKDLGGN AKCSDFTEEI CRRVKDLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and DBA/2. Tissue: Bone marrow, Heart, Tongue, Visual cortex and Wolffian duct.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Liver.
[3]	Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 59-85; 101-188; 206-214 AND 300-336, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK003393 mRNA. Translation: BAB22760.1. AK010065 mRNA. Translation: BAB26679.1. AK032787 mRNA. Translation: BAC28021.1. AK047951 mRNA. Translation: BAC33199.1. AK150618 mRNA. Translation: BAE29708.1. AK151304 mRNA. Translation: BAE30286.1. AK152353 mRNA. Translation: BAE31145.1. AK153459 mRNA. Translation: BAE32011.1. AK158646 mRNA. Translation: BAE34596.1. AK159051 mRNA. Translation: BAE34785.1. AK168049 mRNA. Translation: BAE40031.1. AK168149 mRNA. Translation: BAE40114.1. AK169152 mRNA. Translation: BAE40931.1. BC034273 mRNA. Translation: AAH34273.1. BC049956 mRNA. Translation: AAH49956.1.
IPI	IPI00459725. IPI00608078.
RefSeq	NP_083849.1.
UniGene	Mm.279195
3D structure databases
HSSP	HSSP built from PDB template 1ISO based on UniProtKB P08200.
ModBase	Search...
PTM databases
PhosphoSite	Q9D6R2.
2-D gel databases
REPRODUCTION-2DPAGE	Q9D6R2.
Proteomic databases
PRIDE	Q9D6R2.
Genome annotation databases
Ensembl	ENSMUSG00000032279. Mus musculus. [Contig view]
GeneID	67834.
KEGG	mmu:67834.
NMPDR	fig|10090.3.peg.20219.
Organism-specific databases
MGI	MGI:1915084. Idh3a.
Phylogenomic databases
HOGENOM	Q9D6R2.
HOVERGEN	Q9D6R2.
OMA	Q9D6R2. GGNSKCS.
Enzyme and pathway databases
BRENDA	1.1.1.41. 244.
Gene expression databases
Bgee	Q9D6R2.
CleanEx	MM_IDH3A.
GermOnline	ENSMUSG00000032279. Mus musculus.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	325657.
SOURCE	Search...

Entry information

Entry name

IDH3A_MOUSE

Accession

Primary (citable) accession number: Q9D6R2
Secondary accession number(s): Q3UAM8, Q8C8A1, Q9D1L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)