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Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

Idh3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151SubstrateBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Sitei153 – 1531Critical for catalysisBy similarity
Sitei200 – 2001Critical for catalysisBy similarity
Metal bindingi233 – 2331Magnesium or manganeseBy similarity
Binding sitei233 – 2331SubstrateBy similarity
Metal bindingi257 – 2571Magnesium or manganeseBy similarity
Metal bindingi261 – 2611Magnesium or manganeseBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

ReactomeiREACT_333358. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene namesi
Name:Idh3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1915084. Idh3a.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 366339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771N6-succinyllysine1 Publication
Modified residuei223 – 2231N6-acetyllysine1 Publication
Modified residuei343 – 3431N6-acetyllysine; alternateBy similarity
Modified residuei343 – 3431N6-succinyllysine; alternate1 Publication
Modified residuei350 – 3501N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9D6R2.
PaxDbiQ9D6R2.
PRIDEiQ9D6R2.

2D gel databases

REPRODUCTION-2DPAGEQ9D6R2.
UCD-2DPAGEQ9D6R2.

PTM databases

PhosphoSiteiQ9D6R2.

Expressioni

Gene expression databases

BgeeiQ9D6R2.
CleanExiMM_IDH3A.
GenevisibleiQ9D6R2. MM.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

BioGridi212466. 1 interaction.
IntActiQ9D6R2. 8 interactions.
MINTiMINT-1861233.
STRINGi10090.ENSMUSP00000127526.

Structurei

3D structure databases

ProteinModelPortaliQ9D6R2.
SMRiQ9D6R2. Positions 30-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00550000074918.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ9D6R2.
KOiK00030.
OMAiQITTFAY.
OrthoDBiEOG75B85R.
PhylomeDBiQ9D6R2.
TreeFamiTF105692.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6R2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV
60 70 80 90 100
MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK
110 120 130 140 150
TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE
160 170 180 190 200
GEYSGIEHVI VDGVVQSIKL ITEEASKRIA EFAFEYARNN HRSNVTAVHK
210 220 230 240 250
ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV QDPSQFDVLV
260 270 280 290 300
MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD
310 320 330 340 350
MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNAK
360
CSDFTEEICR RVKDLD
Length:366
Mass (Da):39,639
Last modified:June 1, 2001 - v1
Checksum:i9F1D68C269376955
GO
Isoform 2 (identifier: Q9D6R2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:288
Mass (Da):31,462
Checksum:i5A6F91BF1BBD4FC5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 3061A → T in BAC33199 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7878Missing in isoform 2. 2 PublicationsVSP_014517Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003393 mRNA. Translation: BAB22760.1.
AK010065 mRNA. Translation: BAB26679.1.
AK032787 mRNA. Translation: BAC28021.1.
AK047951 mRNA. Translation: BAC33199.1.
AK150618 mRNA. Translation: BAE29708.1.
AK151304 mRNA. Translation: BAE30286.1.
AK152353 mRNA. Translation: BAE31145.1.
AK153459 mRNA. Translation: BAE32011.1.
AK158646 mRNA. Translation: BAE34596.1.
AK159051 mRNA. Translation: BAE34785.1.
AK168049 mRNA. Translation: BAE40031.1.
AK168149 mRNA. Translation: BAE40114.1.
AK169152 mRNA. Translation: BAE40931.1.
BC034273 mRNA. Translation: AAH34273.1.
BC049956 mRNA. Translation: AAH49956.1.
CCDSiCCDS23191.1. [Q9D6R2-1]
RefSeqiNP_083849.1. NM_029573.2. [Q9D6R2-1]
UniGeneiMm.279195.

Genome annotation databases

EnsembliENSMUST00000167866; ENSMUSP00000127526; ENSMUSG00000032279. [Q9D6R2-1]
GeneIDi67834.
KEGGimmu:67834.
UCSCiuc009prg.1. mouse. [Q9D6R2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003393 mRNA. Translation: BAB22760.1.
AK010065 mRNA. Translation: BAB26679.1.
AK032787 mRNA. Translation: BAC28021.1.
AK047951 mRNA. Translation: BAC33199.1.
AK150618 mRNA. Translation: BAE29708.1.
AK151304 mRNA. Translation: BAE30286.1.
AK152353 mRNA. Translation: BAE31145.1.
AK153459 mRNA. Translation: BAE32011.1.
AK158646 mRNA. Translation: BAE34596.1.
AK159051 mRNA. Translation: BAE34785.1.
AK168049 mRNA. Translation: BAE40031.1.
AK168149 mRNA. Translation: BAE40114.1.
AK169152 mRNA. Translation: BAE40931.1.
BC034273 mRNA. Translation: AAH34273.1.
BC049956 mRNA. Translation: AAH49956.1.
CCDSiCCDS23191.1. [Q9D6R2-1]
RefSeqiNP_083849.1. NM_029573.2. [Q9D6R2-1]
UniGeneiMm.279195.

3D structure databases

ProteinModelPortaliQ9D6R2.
SMRiQ9D6R2. Positions 30-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212466. 1 interaction.
IntActiQ9D6R2. 8 interactions.
MINTiMINT-1861233.
STRINGi10090.ENSMUSP00000127526.

PTM databases

PhosphoSiteiQ9D6R2.

2D gel databases

REPRODUCTION-2DPAGEQ9D6R2.
UCD-2DPAGEQ9D6R2.

Proteomic databases

MaxQBiQ9D6R2.
PaxDbiQ9D6R2.
PRIDEiQ9D6R2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000167866; ENSMUSP00000127526; ENSMUSG00000032279. [Q9D6R2-1]
GeneIDi67834.
KEGGimmu:67834.
UCSCiuc009prg.1. mouse. [Q9D6R2-1]

Organism-specific databases

CTDi3419.
MGIiMGI:1915084. Idh3a.

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00550000074918.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ9D6R2.
KOiK00030.
OMAiQITTFAY.
OrthoDBiEOG75B85R.
PhylomeDBiQ9D6R2.
TreeFamiTF105692.

Enzyme and pathway databases

ReactomeiREACT_333358. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi325657.
PROiQ9D6R2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6R2.
CleanExiMM_IDH3A.
GenevisibleiQ9D6R2. MM.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and DBA/2.
    Tissue: Bone marrow, Heart, Tongue, Visual cortex and Wolffian duct.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Liver.
  3. Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 59-85; 101-188; 206-214 AND 300-336, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-77; LYS-343 AND LYS-350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIDH3A_MOUSE
AccessioniPrimary (citable) accession number: Q9D6R2
Secondary accession number(s): Q3UAM8, Q8C8A1, Q9D1L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.