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Q9D6K9

- CERS5_MOUSE

UniProt

Q9D6K9 - CERS5_MOUSE

Protein

Ceramide synthase 5

Gene

Cers5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Dihydroceramide synthase. Catalyzes the acylation of sphingosine to form dihydroceramide, with high selectivity toward palmitoyl-CoA as acyl donor compared to stearoyl-CoA. Inhibited by fumonisin B1.2 Publications

    Catalytic activityi

    Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi75 – 13662HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. sphingosine N-acyltransferase activity Source: HGNC

    GO - Biological processi

    1. ceramide biosynthetic process Source: HGNC
    2. sphingolipid biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BRENDAi2.3.1.24. 3474.
    ReactomeiREACT_198151. Sphingolipid de novo biosynthesis.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ceramide synthase 5 (EC:2.3.1.24)
    Short name:
    CerS5
    Alternative name(s):
    LAG1 longevity assurance homolog 5
    Translocating chain-associating membrane protein homolog 4
    Short name:
    TRAM homolog 4
    Gene namesi
    Name:Cers5
    Synonyms:Lass5, Trh4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1919199. Cers5.

    Subcellular locationi

    Nucleus membrane PROSITE-ProRule annotation; Multi-pass membrane protein Curated. Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 414414Ceramide synthase 5PRO_0000185515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi26 – 261N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9D6K9.
    PRIDEiQ9D6K9.

    PTM databases

    PhosphoSiteiQ9D6K9.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed, with highest levels in testis and kidney.2 Publications

    Gene expression databases

    ArrayExpressiQ9D6K9.
    BgeeiQ9D6K9.
    CleanExiMM_LASS5.
    GenevestigatoriQ9D6K9.

    Interactioni

    Protein-protein interaction databases

    BioGridi215049. 3 interactions.
    MINTiMINT-4998087.

    Structurei

    Secondary structure

    1
    414
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi91 – 988
    Helixi105 – 11410
    Helixi119 – 13315

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CQXNMR-A77-135[»]
    ProteinModelPortaliQ9D6K9.
    SMRiQ9D6K9. Positions 78-135.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9D6K9.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4343LumenalSequence AnalysisAdd
    BLAST
    Topological domaini65 – 14783CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini169 – 18618LumenalSequence AnalysisAdd
    BLAST
    Topological domaini208 – 2136CytoplasmicSequence Analysis
    Topological domaini235 – 27137LumenalSequence AnalysisAdd
    BLAST
    Topological domaini293 – 31119CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini333 – 41482LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei44 – 6421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei187 – 20721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei214 – 23421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei272 – 29221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei312 – 33221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini139 – 340202TLCPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5058.
    GeneTreeiENSGT00550000074401.
    HOVERGENiHBG052310.
    InParanoidiQ9D6K9.
    KOiK04710.
    OMAiGGSYWAE.
    OrthoDBiEOG7CZK7X.
    PhylomeDBiQ9D6K9.
    TreeFamiTF314319.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR016439. Longevity_assurance_LAG1_LAC1.
    IPR006634. TLC-dom.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF03798. TRAM_LAG1_CLN8. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
    SMARTiSM00389. HOX. 1 hit.
    SM00724. TLC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS50071. HOMEOBOX_2. 1 hit.
    PS50922. TLC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9D6K9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV    50
    FPLAVCIFSV RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT 100
    KYPDEKRLKG LSKQLDWSVR KIQCWFRHRR NQDKPPTLTK FCESMWRFTY 150
    YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY QPLSRELYYY YITQLAFYWS 200
    LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG ALIFCLHDFA 250
    DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES 300
    WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS 350
    DVESSSEEED ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG 400
    NLHFRASPHL HSCD 414
    Length:414
    Mass (Da):48,167
    Last modified:June 1, 2001 - v1
    Checksum:iE3536F344A459D49
    GO
    Isoform 2 (identifier: Q9D6K9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         345-387: SKDDRSDVES...NCMNGYMGGS → LSQEGLDPGS...CMCLRMTAVM
         388-414: Missing.

    Show »
    Length:387
    Mass (Da):45,428
    Checksum:i61692188487B3BC2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821P → A in AAK40301. 1 PublicationCurated
    Sequence conflicti310 – 3101W → R in BAC35894. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei345 – 38743SKDDR…YMGGS → LSQEGLDPGSVPSALSHLLP LFSAWWQLDSSILCMCLRMT AVM in isoform 2. 1 PublicationVSP_011192Add
    BLAST
    Alternative sequencei388 – 41427Missing in isoform 2. 1 PublicationVSP_011193Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY029533 mRNA. Translation: AAK40301.1.
    AK010241 mRNA. Translation: BAB26792.1.
    AK075694 mRNA. Translation: BAC35894.1.
    AK145589 mRNA. Translation: BAE26527.1.
    AK145762 mRNA. Translation: BAE26634.1.
    BC010670 mRNA. Translation: AAH10670.1.
    BC043059 mRNA. Translation: AAH43059.1.
    BC046797 mRNA. Translation: AAH46797.1.
    CCDSiCCDS27830.1. [Q9D6K9-1]
    RefSeqiNP_082291.1. NM_028015.2. [Q9D6K9-1]
    UniGeneiMm.390821.

    Genome annotation databases

    EnsembliENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021. [Q9D6K9-1]
    ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021. [Q9D6K9-2]
    GeneIDi71949.
    KEGGimmu:71949.
    UCSCiuc007xqf.1. mouse. [Q9D6K9-1]
    uc007xqg.1. mouse. [Q9D6K9-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY029533 mRNA. Translation: AAK40301.1 .
    AK010241 mRNA. Translation: BAB26792.1 .
    AK075694 mRNA. Translation: BAC35894.1 .
    AK145589 mRNA. Translation: BAE26527.1 .
    AK145762 mRNA. Translation: BAE26634.1 .
    BC010670 mRNA. Translation: AAH10670.1 .
    BC043059 mRNA. Translation: AAH43059.1 .
    BC046797 mRNA. Translation: AAH46797.1 .
    CCDSi CCDS27830.1. [Q9D6K9-1 ]
    RefSeqi NP_082291.1. NM_028015.2. [Q9D6K9-1 ]
    UniGenei Mm.390821.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CQX NMR - A 77-135 [» ]
    ProteinModelPortali Q9D6K9.
    SMRi Q9D6K9. Positions 78-135.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215049. 3 interactions.
    MINTi MINT-4998087.

    PTM databases

    PhosphoSitei Q9D6K9.

    Proteomic databases

    PaxDbi Q9D6K9.
    PRIDEi Q9D6K9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023762 ; ENSMUSP00000023762 ; ENSMUSG00000023021 . [Q9D6K9-1 ]
    ENSMUST00000109035 ; ENSMUSP00000104663 ; ENSMUSG00000023021 . [Q9D6K9-2 ]
    GeneIDi 71949.
    KEGGi mmu:71949.
    UCSCi uc007xqf.1. mouse. [Q9D6K9-1 ]
    uc007xqg.1. mouse. [Q9D6K9-2 ]

    Organism-specific databases

    CTDi 91012.
    MGIi MGI:1919199. Cers5.

    Phylogenomic databases

    eggNOGi COG5058.
    GeneTreei ENSGT00550000074401.
    HOVERGENi HBG052310.
    InParanoidi Q9D6K9.
    KOi K04710.
    OMAi GGSYWAE.
    OrthoDBi EOG7CZK7X.
    PhylomeDBi Q9D6K9.
    TreeFami TF314319.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    BRENDAi 2.3.1.24. 3474.
    Reactomei REACT_198151. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    EvolutionaryTracei Q9D6K9.
    NextBioi 335024.
    PROi Q9D6K9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D6K9.
    Bgeei Q9D6K9.
    CleanExi MM_LASS5.
    Genevestigatori Q9D6K9.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR016439. Longevity_assurance_LAG1_LAC1.
    IPR006634. TLC-dom.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF03798. TRAM_LAG1_CLN8. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005225. LAG1_LAC1. 1 hit.
    SMARTi SM00389. HOX. 1 hit.
    SM00724. TLC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS50071. HOMEOBOX_2. 1 hit.
    PS50922. TLC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Hartmann E.
      Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Tongue.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Brain and Mammary tumor.
    4. "Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors."
      Riebeling C., Allegood J.C., Wang E., Merrill A.H. Jr., Futerman A.H.
      J. Biol. Chem. 278:43452-43459(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CERAMIDE SYNTHESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    5. "Mammalian Lass6 and its related family members regulate synthesis of specific ceramides."
      Mizutani Y., Kihara A., Igarashi Y.
      Biochem. J. 390:263-271(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-26, TISSUE SPECIFICITY.
    6. "LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor."
      Lahiri S., Futerman A.H.
      J. Biol. Chem. 280:33735-33738(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Solution structure of RSGI RUH-034, a homeodomain from mouse cDNA."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 77-135.

    Entry informationi

    Entry nameiCERS5_MOUSE
    AccessioniPrimary (citable) accession number: Q9D6K9
    Secondary accession number(s): Q3UL17
    , Q80YB2, Q8BPH6, Q921T8, Q924Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3