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Protein

Ceramide synthase 5

Gene

Cers5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dihydroceramide synthase. Catalyzes the acylation of sphingosine to form dihydroceramide, with high selectivity toward palmitoyl-CoA as acyl donor compared to stearoyl-CoA. Inhibited by fumonisin B1.2 Publications

Catalytic activityi

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi75 – 136HomeoboxPROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • sphingosine N-acyltransferase activity Source: HGNC

GO - Biological processi

  • ceramide biosynthetic process Source: HGNC
  • sphingolipid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BRENDAi2.3.1.24. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.
UniPathwayiUPA00222.

Chemistry databases

SwissLipidsiSLP:000000118.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide synthase 5 (EC:2.3.1.24)
Short name:
CerS5
Alternative name(s):
LAG1 longevity assurance homolog 5
Translocating chain-associating membrane protein homolog 4
Short name:
TRAM homolog 4
Gene namesi
Name:Cers5
Synonyms:Lass5, Trh4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1919199. Cers5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43LumenalSequence analysisAdd BLAST43
Transmembranei44 – 64HelicalSequence analysisAdd BLAST21
Topological domaini65 – 147CytoplasmicSequence analysisAdd BLAST83
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 186LumenalSequence analysisAdd BLAST18
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 213CytoplasmicSequence analysis6
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 271LumenalSequence analysisAdd BLAST37
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 311CytoplasmicSequence analysisAdd BLAST19
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 414LumenalSequence analysisAdd BLAST82

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001855151 – 414Ceramide synthase 5Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi26N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9D6K9.
PaxDbiQ9D6K9.
PeptideAtlasiQ9D6K9.
PRIDEiQ9D6K9.

PTM databases

iPTMnetiQ9D6K9.
PhosphoSitePlusiQ9D6K9.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in testis and kidney.2 Publications

Gene expression databases

BgeeiENSMUSG00000023021.
CleanExiMM_LASS5.
ExpressionAtlasiQ9D6K9. baseline and differential.
GenevisibleiQ9D6K9. MM.

Interactioni

Protein-protein interaction databases

BioGridi215049. 3 interactors.
MINTiMINT-4998087.
STRINGi10090.ENSMUSP00000023762.

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi91 – 98Combined sources8
Helixi105 – 114Combined sources10
Helixi119 – 133Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQXNMR-A77-135[»]
5E8NX-ray2.25C/F/I/L379-387[»]
5E8OX-ray1.98C/F379-387[»]
5E8PX-ray2.00C/F379-387[»]
ProteinModelPortaliQ9D6K9.
SMRiQ9D6K9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D6K9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 340TLCPROSITE-ProRule annotationAdd BLAST202

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1607. Eukaryota.
COG5058. LUCA.
GeneTreeiENSGT00550000074401.
HOVERGENiHBG052310.
InParanoidiQ9D6K9.
KOiK04710.
OMAiSSNGANW.
OrthoDBiEOG091G0BWM.
PhylomeDBiQ9D6K9.
TreeFamiTF314319.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6K9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV
60 70 80 90 100
FPLAVCIFSV RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT
110 120 130 140 150
KYPDEKRLKG LSKQLDWSVR KIQCWFRHRR NQDKPPTLTK FCESMWRFTY
160 170 180 190 200
YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY QPLSRELYYY YITQLAFYWS
210 220 230 240 250
LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG ALIFCLHDFA
260 270 280 290 300
DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES
310 320 330 340 350
WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS
360 370 380 390 400
DVESSSEEED ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG
410
NLHFRASPHL HSCD
Length:414
Mass (Da):48,167
Last modified:June 1, 2001 - v1
Checksum:iE3536F344A459D49
GO
Isoform 2 (identifier: Q9D6K9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     345-387: SKDDRSDVES...NCMNGYMGGS → LSQEGLDPGS...CMCLRMTAVM
     388-414: Missing.

Show »
Length:387
Mass (Da):45,428
Checksum:i61692188487B3BC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82P → A in AAK40301 (Ref. 1) Curated1
Sequence conflicti310W → R in BAC35894 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011192345 – 387SKDDR…YMGGS → LSQEGLDPGSVPSALSHLLP LFSAWWQLDSSILCMCLRMT AVM in isoform 2. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_011193388 – 414Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029533 mRNA. Translation: AAK40301.1.
AK010241 mRNA. Translation: BAB26792.1.
AK075694 mRNA. Translation: BAC35894.1.
AK145589 mRNA. Translation: BAE26527.1.
AK145762 mRNA. Translation: BAE26634.1.
BC010670 mRNA. Translation: AAH10670.1.
BC043059 mRNA. Translation: AAH43059.1.
BC046797 mRNA. Translation: AAH46797.1.
CCDSiCCDS27830.1. [Q9D6K9-1]
RefSeqiNP_082291.1. NM_028015.2. [Q9D6K9-1]
UniGeneiMm.390821.

Genome annotation databases

EnsembliENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021. [Q9D6K9-1]
ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021. [Q9D6K9-2]
GeneIDi71949.
KEGGimmu:71949.
UCSCiuc007xqf.1. mouse. [Q9D6K9-1]
uc007xqg.1. mouse. [Q9D6K9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029533 mRNA. Translation: AAK40301.1.
AK010241 mRNA. Translation: BAB26792.1.
AK075694 mRNA. Translation: BAC35894.1.
AK145589 mRNA. Translation: BAE26527.1.
AK145762 mRNA. Translation: BAE26634.1.
BC010670 mRNA. Translation: AAH10670.1.
BC043059 mRNA. Translation: AAH43059.1.
BC046797 mRNA. Translation: AAH46797.1.
CCDSiCCDS27830.1. [Q9D6K9-1]
RefSeqiNP_082291.1. NM_028015.2. [Q9D6K9-1]
UniGeneiMm.390821.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQXNMR-A77-135[»]
5E8NX-ray2.25C/F/I/L379-387[»]
5E8OX-ray1.98C/F379-387[»]
5E8PX-ray2.00C/F379-387[»]
ProteinModelPortaliQ9D6K9.
SMRiQ9D6K9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215049. 3 interactors.
MINTiMINT-4998087.
STRINGi10090.ENSMUSP00000023762.

Chemistry databases

SwissLipidsiSLP:000000118.

PTM databases

iPTMnetiQ9D6K9.
PhosphoSitePlusiQ9D6K9.

Proteomic databases

EPDiQ9D6K9.
PaxDbiQ9D6K9.
PeptideAtlasiQ9D6K9.
PRIDEiQ9D6K9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021. [Q9D6K9-1]
ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021. [Q9D6K9-2]
GeneIDi71949.
KEGGimmu:71949.
UCSCiuc007xqf.1. mouse. [Q9D6K9-1]
uc007xqg.1. mouse. [Q9D6K9-2]

Organism-specific databases

CTDi91012.
MGIiMGI:1919199. Cers5.

Phylogenomic databases

eggNOGiKOG1607. Eukaryota.
COG5058. LUCA.
GeneTreeiENSGT00550000074401.
HOVERGENiHBG052310.
InParanoidiQ9D6K9.
KOiK04710.
OMAiSSNGANW.
OrthoDBiEOG091G0BWM.
PhylomeDBiQ9D6K9.
TreeFamiTF314319.

Enzyme and pathway databases

UniPathwayiUPA00222.
BRENDAi2.3.1.24. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

EvolutionaryTraceiQ9D6K9.
PROiQ9D6K9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023021.
CleanExiMM_LASS5.
ExpressionAtlasiQ9D6K9. baseline and differential.
GenevisibleiQ9D6K9. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCERS5_MOUSE
AccessioniPrimary (citable) accession number: Q9D6K9
Secondary accession number(s): Q3UL17
, Q80YB2, Q8BPH6, Q921T8, Q924Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.