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Q9D6K9 (CERS5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ceramide synthase 5

Short name=CerS5
EC=2.3.1.24
Alternative name(s):
LAG1 longevity assurance homolog 5
Translocating chain-associating membrane protein homolog 4
Short name=TRAM homolog 4
Gene names
Name:Cers5
Synonyms:Lass5, Trh4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dihydroceramide synthase. Catalyzes the acylation of sphingosine to form dihydroceramide, with high selectivity toward palmitoyl-CoA as acyl donor compared to stearoyl-CoA. Inhibited by fumonisin B1. Ref.4 Ref.6

Catalytic activity

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

Pathway

Lipid metabolism; sphingolipid metabolism.

Subcellular location

Nucleus membrane; Multi-pass membrane protein Potential. Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.

Tissue specificity

Ubiquitously expressed, with highest levels in testis and kidney. Ref.4 Ref.5

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9D6K9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9D6K9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     345-387: SKDDRSDVES...NCMNGYMGGS → LSQEGLDPGS...CMCLRMTAVM
     388-414: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Ceramide synthase 5
PRO_0000185515

Regions

Topological domain1 – 4343Lumenal Potential
Transmembrane44 – 6421Helical; Potential
Topological domain65 – 14783Cytoplasmic Potential
Transmembrane148 – 16821Helical; Potential
Topological domain169 – 18618Lumenal Potential
Transmembrane187 – 20721Helical; Potential
Topological domain208 – 2136Cytoplasmic Potential
Transmembrane214 – 23421Helical; Potential
Topological domain235 – 27137Lumenal Potential
Transmembrane272 – 29221Helical; Potential
Topological domain293 – 31119Cytoplasmic Potential
Transmembrane312 – 33221Helical; Potential
Topological domain333 – 41482Lumenal Potential
Domain139 – 340202TLC
DNA binding75 – 13662Homeobox

Amino acid modifications

Glycosylation261N-linked (GlcNAc...) Ref.5

Natural variations

Alternative sequence345 – 38743SKDDR…YMGGS → LSQEGLDPGSVPSALSHLLP LFSAWWQLDSSILCMCLRMT AVM in isoform 2.
VSP_011192
Alternative sequence388 – 41427Missing in isoform 2.
VSP_011193

Experimental info

Sequence conflict821P → A in AAK40301. Ref.1
Sequence conflict3101W → R in BAC35894. Ref.2

Secondary structure

....... 414
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: E3536F344A459D49

FASTA41448,167
        10         20         30         40         50         60 
MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV FPLAVCIFSV 

        70         80         90        100        110        120 
RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT KYPDEKRLKG LSKQLDWSVR 

       130        140        150        160        170        180 
KIQCWFRHRR NQDKPPTLTK FCESMWRFTY YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY 

       190        200        210        220        230        240 
QPLSRELYYY YITQLAFYWS LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG 

       250        260        270        280        290        300 
ALIFCLHDFA DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES 

       310        320        330        340        350        360 
WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS DVESSSEEED 

       370        380        390        400        410 
ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG NLHFRASPHL HSCD 

« Hide

Isoform 2 [UniParc].

Checksum: 61692188487B3BC2
Show »

FASTA38745,428

References

« Hide 'large scale' references
[1]Hartmann E.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Tongue.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain and Mammary tumor.
[4]"Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors."
Riebeling C., Allegood J.C., Wang E., Merrill A.H. Jr., Futerman A.H.
J. Biol. Chem. 278:43452-43459(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN CERAMIDE SYNTHESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"Mammalian Lass6 and its related family members regulate synthesis of specific ceramides."
Mizutani Y., Kihara A., Igarashi Y.
Biochem. J. 390:263-271(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-26, TISSUE SPECIFICITY.
[6]"LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor."
Lahiri S., Futerman A.H.
J. Biol. Chem. 280:33735-33738(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Solution structure of RSGI RUH-034, a homeodomain from mouse cDNA."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 77-135.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY029533 mRNA. Translation: AAK40301.1.
AK010241 mRNA. Translation: BAB26792.1.
AK075694 mRNA. Translation: BAC35894.1.
AK145589 mRNA. Translation: BAE26527.1.
AK145762 mRNA. Translation: BAE26634.1.
BC010670 mRNA. Translation: AAH10670.1.
BC043059 mRNA. Translation: AAH43059.1.
BC046797 mRNA. Translation: AAH46797.1.
CCDSCCDS27830.1. [Q9D6K9-1]
RefSeqNP_082291.1. NM_028015.2. [Q9D6K9-1]
UniGeneMm.390821.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CQXNMR-A77-135[»]
ProteinModelPortalQ9D6K9.
SMRQ9D6K9. Positions 78-135.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid215049. 3 interactions.
MINTMINT-4998087.

PTM databases

PhosphoSiteQ9D6K9.

Proteomic databases

PaxDbQ9D6K9.
PRIDEQ9D6K9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021. [Q9D6K9-1]
ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021. [Q9D6K9-2]
GeneID71949.
KEGGmmu:71949.
UCSCuc007xqf.1. mouse. [Q9D6K9-1]
uc007xqg.1. mouse. [Q9D6K9-2]

Organism-specific databases

CTD91012.
MGIMGI:1919199. Cers5.

Phylogenomic databases

eggNOGCOG5058.
GeneTreeENSGT00550000074401.
HOVERGENHBG052310.
InParanoidQ9D6K9.
KOK04710.
OMAGGSYWAE.
OrthoDBEOG7CZK7X.
PhylomeDBQ9D6K9.
TreeFamTF314319.

Enzyme and pathway databases

BRENDA2.3.1.24. 3474.
UniPathwayUPA00222.

Gene expression databases

ArrayExpressQ9D6K9.
BgeeQ9D6K9.
CleanExMM_LASS5.
GenevestigatorQ9D6K9.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFPIRSF005225. LAG1_LAC1. 1 hit.
SMARTSM00389. HOX. 1 hit.
SM00724. TLC. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9D6K9.
NextBio335024.
PROQ9D6K9.
SOURCESearch...

Entry information

Entry nameCERS5_MOUSE
AccessionPrimary (citable) accession number: Q9D6K9
Secondary accession number(s): Q3UL17 expand/collapse secondary AC list , Q80YB2, Q8BPH6, Q921T8, Q924Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot