Q9D6K9 (CERS5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ceramide synthase 5
Short name=CerS5

Alternative name(s):
LAG1 longevity assurance homolog 5
Translocating chain-associating membrane protein homolog 4
Short name=TRAM homolog 4

Gene names

Name:	Cers5
Synonyms:	Lass5, Trh4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl- (C16) ceramide) in a fumonisin B1-independent manner. Ref.4
Subcellular location	Nucleus membrane; Multi-pass membrane protein Potential. Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.
Tissue specificity	Ubiquitously expressed, with highest levels in testis and kidney. Ref.4 Ref.5
Sequence similarities	Contains 1 homeobox DNA-binding domain. Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
Biological process	Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox Transmembrane Transmembrane helix
Ligand	DNA-binding
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide biosynthetic process Inferred from direct assay. Source: HGNC
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from sequence or structural similarity. Source: HGNC nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sphingosine N-acyltransferase activity Inferred from direct assay. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D6K9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9D6K9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     345-387: SKDDRSDVES...NCMNGYMGGS → LSQEGLDPGS...CMCLRMTAVM
     388-414: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 414

414

Ceramide synthase 5

PRO_0000185515

Regions

Topological domain

1 – 43

43

Lumenal Potential

Transmembrane

44 – 64

21

Helical; Potential

Topological domain

65 – 147

83

Cytoplasmic Potential

Transmembrane

148 – 168

21

Helical; Potential

Topological domain

169 – 186

18

Lumenal Potential

Transmembrane

187 – 207

21

Helical; Potential

Topological domain

208 – 213

6

Cytoplasmic Potential

Transmembrane

214 – 234

21

Helical; Potential

Topological domain

235 – 271

37

Lumenal Potential

Transmembrane

272 – 292

21

Helical; Potential

Topological domain

293 – 311

19

Cytoplasmic Potential

Transmembrane

312 – 332

21

Helical; Potential

Topological domain

333 – 414

82

Lumenal Potential

Domain

139 – 340

202

TLC

DNA binding

75 – 136

62

Homeobox

Amino acid modifications

Modified residue

350

1

Phosphoserine By similarity

Modified residue

354

1

Phosphoserine By similarity

Modified residue

355

1

Phosphoserine By similarity

Modified residue

356

1

Phosphoserine By similarity

Glycosylation

26

1

N-linked (GlcNAc...) Ref.5

Natural variations

Alternative sequence

345 – 387

43

SKDDR…YMGGS → LSQEGLDPGSVPSALSHLLP LFSAWWQLDSSILCMCLRMT AVM in isoform 2.

VSP_011192

Alternative sequence

388 – 414

27

Missing in isoform 2.

VSP_011193

Experimental info

Sequence conflict

82

1

P → A in AAK40301. Ref.1

Sequence conflict

310

1

W → R in BAC35894. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

414

Helix Strand Turn

Details...

Helix

91 – 98

8

Helix

105 – 114

10

Helix

119 – 133

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E3536F344A459D49	FASTA	414	48,167
	10 20 30 40 50 60 MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV FPLAVCIFSV 70 80 90 100 110 120 RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT KYPDEKRLKG LSKQLDWSVR 130 140 150 160 170 180 KIQCWFRHRR NQDKPPTLTK FCESMWRFTY YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY 190 200 210 220 230 240 QPLSRELYYY YITQLAFYWS LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG 250 260 270 280 290 300 ALIFCLHDFA DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES 310 320 330 340 350 360 WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS DVESSSEEED 370 380 390 400 410 ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG NLHFRASPHL HSCD
	Isoform 2 [UniParc]. Checksum: 61692188487B3BC2	FASTA	387	45,428
	10 20 30 40 50 60 MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV FPLAVCIFSV 70 80 90 100 110 120 RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT KYPDEKRLKG LSKQLDWSVR 130 140 150 160 170 180 KIQCWFRHRR NQDKPPTLTK FCESMWRFTY YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY 190 200 210 220 230 240 QPLSRELYYY YITQLAFYWS LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG 250 260 270 280 290 300 ALIFCLHDFA DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES 310 320 330 340 350 360 WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVLSQEGL DPGSVPSALS 370 380 HLLPLFSAWW QLDSSILCMC LRMTAVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hartmann E. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Tongue.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Brain and Mammary tumor.
[4]	"Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors." Riebeling C., Allegood J.C., Wang E., Merrill A.H. Jr., Futerman A.H. J. Biol. Chem. 278:43452-43459(2003) [PubMed: 12912983] [Abstract] Cited for: FUNCTION IN CERAMIDE SYNTHESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]	"Mammalian Lass6 and its related family members regulate synthesis of specific ceramides." Mizutani Y., Kihara A., Igarashi Y. Biochem. J. 390:263-271(2005) [PubMed: 15823095] [Abstract] Cited for: GLYCOSYLATION AT ASN-26, TISSUE SPECIFICITY.
[6]	"Solution structure of RSGI RUH-034, a homeodomain from mouse cDNA." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 77-135.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY029533 mRNA. Translation: AAK40301.1.
AK010241 mRNA. Translation: BAB26792.1.
AK075694 mRNA. Translation: BAC35894.1.
AK145589 mRNA. Translation: BAE26527.1.
AK145762 mRNA. Translation: BAE26634.1.
BC010670 mRNA. Translation: AAH10670.1.
BC043059 mRNA. Translation: AAH43059.1.
BC046797 mRNA. Translation: AAH46797.1.

IPI

IPI00109214.
IPI00403908.

RefSeq

NP_082291.1. NM_028015.2.

UniGene

Mm.390821.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CQX	NMR	-	A	77-135	[»]

ProteinModelPortal

Q9D6K9.

SMR

Q9D6K9. Positions 78-135.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9D6K9.

PTM databases

PhosphoSite

Q9D6K9.

Proteomic databases

PRIDE

Q9D6K9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021.
ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021.

GeneID

71949.

KEGG

mmu:71949.

UCSC

uc007xqf.1. mouse.
uc007xqg.1. mouse.

Organism-specific databases

CTD

71949.

MGI

MGI:1919199. Lass5.

Phylogenomic databases

GeneTree

ENSGT00550000074401.

HOGENOM

HBG444829.

HOVERGEN

HBG052310.

InParanoid

Q9D6K9.

OMA

AVFMVTR.

OrthoDB

EOG4NVZKJ.

PhylomeDB

Q9D6K9.

Enzyme and pathway databases

BRENDA

2.3.1.24. 3474.

Gene expression databases

ArrayExpress

Q9D6K9.

Bgee

Q9D6K9.

CleanEx

MM_LASS5.

Genevestigator

Q9D6K9.

GermOnline

ENSMUSG00000023021. Mus musculus.

Family and domain databases

InterPro

IPR001356. Homeobox.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

KO

K04710.

Pfam

PF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]

PIRSF

PIRSF005225. LAG1_LAC1. 1 hit.

SMART

SM00389. HOX. 1 hit.
SM00724. TLC. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

PROSITE

PS00027. HOMEOBOX_1. False negative.
PS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

335024.

SOURCE

Search...

Entry information

Entry name

CERS5_MOUSE

Accession

Primary (citable) accession number: Q9D6K9
Secondary accession number(s): Q3UL17 , Q80YB2, Q8BPH6, Q921T8, Q924Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program