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Q9D6K9

- CERS5_MOUSE

UniProt

Q9D6K9 - CERS5_MOUSE

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Protein

Ceramide synthase 5

Gene

Cers5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Dihydroceramide synthase. Catalyzes the acylation of sphingosine to form dihydroceramide, with high selectivity toward palmitoyl-CoA as acyl donor compared to stearoyl-CoA. Inhibited by fumonisin B1.2 Publications

Catalytic activityi

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi75 – 13662HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sphingosine N-acyltransferase activity Source: HGNC

GO - Biological processi

  1. ceramide biosynthetic process Source: HGNC
  2. sphingolipid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BRENDAi2.3.1.24. 3474.
ReactomeiREACT_198151. Sphingolipid de novo biosynthesis.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide synthase 5 (EC:2.3.1.24)
Short name:
CerS5
Alternative name(s):
LAG1 longevity assurance homolog 5
Translocating chain-associating membrane protein homolog 4
Short name:
TRAM homolog 4
Gene namesi
Name:Cers5
Synonyms:Lass5, Trh4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1919199. Cers5.

Subcellular locationi

Nucleus membrane PROSITE-ProRule annotation; Multi-pass membrane protein Curated. Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Ceramide synthase 5PRO_0000185515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9D6K9.
PaxDbiQ9D6K9.
PRIDEiQ9D6K9.

PTM databases

PhosphoSiteiQ9D6K9.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in testis and kidney.2 Publications

Gene expression databases

BgeeiQ9D6K9.
CleanExiMM_LASS5.
ExpressionAtlasiQ9D6K9. baseline and differential.
GenevestigatoriQ9D6K9.

Interactioni

Protein-protein interaction databases

BioGridi215049. 3 interactions.
MINTiMINT-4998087.

Structurei

Secondary structure

1
414
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi91 – 988
Helixi105 – 11410
Helixi119 – 13315

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CQXNMR-A77-135[»]
ProteinModelPortaliQ9D6K9.
SMRiQ9D6K9. Positions 78-135.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D6K9.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343LumenalSequence AnalysisAdd
BLAST
Topological domaini65 – 14783CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini169 – 18618LumenalSequence AnalysisAdd
BLAST
Topological domaini208 – 2136CytoplasmicSequence Analysis
Topological domaini235 – 27137LumenalSequence AnalysisAdd
BLAST
Topological domaini293 – 31119CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini333 – 41482LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei44 – 6421HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence AnalysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence AnalysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence AnalysisAdd
BLAST
Transmembranei272 – 29221HelicalSequence AnalysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini139 – 340202TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5058.
GeneTreeiENSGT00550000074401.
HOVERGENiHBG052310.
InParanoidiQ9D6K9.
KOiK04710.
OMAiGGSYWAE.
OrthoDBiEOG7CZK7X.
PhylomeDBiQ9D6K9.
TreeFamiTF314319.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00389. HOX. 1 hit.
SM00724. TLC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9D6K9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV
60 70 80 90 100
FPLAVCIFSV RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT
110 120 130 140 150
KYPDEKRLKG LSKQLDWSVR KIQCWFRHRR NQDKPPTLTK FCESMWRFTY
160 170 180 190 200
YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY QPLSRELYYY YITQLAFYWS
210 220 230 240 250
LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG ALIFCLHDFA
260 270 280 290 300
DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES
310 320 330 340 350
WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS
360 370 380 390 400
DVESSSEEED ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG
410
NLHFRASPHL HSCD
Length:414
Mass (Da):48,167
Last modified:June 1, 2001 - v1
Checksum:iE3536F344A459D49
GO
Isoform 2 (identifier: Q9D6K9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     345-387: SKDDRSDVES...NCMNGYMGGS → LSQEGLDPGS...CMCLRMTAVM
     388-414: Missing.

Show »
Length:387
Mass (Da):45,428
Checksum:i61692188487B3BC2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821P → A in AAK40301. 1 PublicationCurated
Sequence conflicti310 – 3101W → R in BAC35894. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei345 – 38743SKDDR…YMGGS → LSQEGLDPGSVPSALSHLLP LFSAWWQLDSSILCMCLRMT AVM in isoform 2. 1 PublicationVSP_011192Add
BLAST
Alternative sequencei388 – 41427Missing in isoform 2. 1 PublicationVSP_011193Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY029533 mRNA. Translation: AAK40301.1.
AK010241 mRNA. Translation: BAB26792.1.
AK075694 mRNA. Translation: BAC35894.1.
AK145589 mRNA. Translation: BAE26527.1.
AK145762 mRNA. Translation: BAE26634.1.
BC010670 mRNA. Translation: AAH10670.1.
BC043059 mRNA. Translation: AAH43059.1.
BC046797 mRNA. Translation: AAH46797.1.
CCDSiCCDS27830.1. [Q9D6K9-1]
RefSeqiNP_082291.1. NM_028015.2. [Q9D6K9-1]
UniGeneiMm.390821.

Genome annotation databases

EnsembliENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021. [Q9D6K9-1]
ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021. [Q9D6K9-2]
GeneIDi71949.
KEGGimmu:71949.
UCSCiuc007xqf.1. mouse. [Q9D6K9-1]
uc007xqg.1. mouse. [Q9D6K9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY029533 mRNA. Translation: AAK40301.1 .
AK010241 mRNA. Translation: BAB26792.1 .
AK075694 mRNA. Translation: BAC35894.1 .
AK145589 mRNA. Translation: BAE26527.1 .
AK145762 mRNA. Translation: BAE26634.1 .
BC010670 mRNA. Translation: AAH10670.1 .
BC043059 mRNA. Translation: AAH43059.1 .
BC046797 mRNA. Translation: AAH46797.1 .
CCDSi CCDS27830.1. [Q9D6K9-1 ]
RefSeqi NP_082291.1. NM_028015.2. [Q9D6K9-1 ]
UniGenei Mm.390821.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CQX NMR - A 77-135 [» ]
ProteinModelPortali Q9D6K9.
SMRi Q9D6K9. Positions 78-135.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 215049. 3 interactions.
MINTi MINT-4998087.

PTM databases

PhosphoSitei Q9D6K9.

Proteomic databases

MaxQBi Q9D6K9.
PaxDbi Q9D6K9.
PRIDEi Q9D6K9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023762 ; ENSMUSP00000023762 ; ENSMUSG00000023021 . [Q9D6K9-1 ]
ENSMUST00000109035 ; ENSMUSP00000104663 ; ENSMUSG00000023021 . [Q9D6K9-2 ]
GeneIDi 71949.
KEGGi mmu:71949.
UCSCi uc007xqf.1. mouse. [Q9D6K9-1 ]
uc007xqg.1. mouse. [Q9D6K9-2 ]

Organism-specific databases

CTDi 91012.
MGIi MGI:1919199. Cers5.

Phylogenomic databases

eggNOGi COG5058.
GeneTreei ENSGT00550000074401.
HOVERGENi HBG052310.
InParanoidi Q9D6K9.
KOi K04710.
OMAi GGSYWAE.
OrthoDBi EOG7CZK7X.
PhylomeDBi Q9D6K9.
TreeFami TF314319.

Enzyme and pathway databases

UniPathwayi UPA00222 .
BRENDAi 2.3.1.24. 3474.
Reactomei REACT_198151. Sphingolipid de novo biosynthesis.

Miscellaneous databases

EvolutionaryTracei Q9D6K9.
NextBioi 335024.
PROi Q9D6K9.
SOURCEi Search...

Gene expression databases

Bgeei Q9D6K9.
CleanExi MM_LASS5.
ExpressionAtlasi Q9D6K9. baseline and differential.
Genevestigatori Q9D6K9.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view ]
PIRSFi PIRSF005225. LAG1_LAC1. 1 hit.
SMARTi SM00389. HOX. 1 hit.
SM00724. TLC. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Hartmann E.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Tongue.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Brain and Mammary tumor.
  4. "Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors."
    Riebeling C., Allegood J.C., Wang E., Merrill A.H. Jr., Futerman A.H.
    J. Biol. Chem. 278:43452-43459(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CERAMIDE SYNTHESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  5. "Mammalian Lass6 and its related family members regulate synthesis of specific ceramides."
    Mizutani Y., Kihara A., Igarashi Y.
    Biochem. J. 390:263-271(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-26, TISSUE SPECIFICITY.
  6. "LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor."
    Lahiri S., Futerman A.H.
    J. Biol. Chem. 280:33735-33738(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Solution structure of RSGI RUH-034, a homeodomain from mouse cDNA."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 77-135.

Entry informationi

Entry nameiCERS5_MOUSE
AccessioniPrimary (citable) accession number: Q9D6K9
Secondary accession number(s): Q3UL17
, Q80YB2, Q8BPH6, Q921T8, Q924Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3