Reviewed, UniProtKB/Swiss-Prot Q9D6K5 (SYJ2B_MOUSE)

Names and origin

Protein names

Recommended name:
    Synaptojanin-2-binding protein
Alternative name(s):
    Activin receptor-interacting protein 2
    Activin receptor-interacting protein 4
    Mitochondrial outer membrane protein 25

Gene names

Name:	Synj2bp
Synonyms:	Arip2, Omp25

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Isoform 1 regulates endocytosis of activin type 2 receptor kinases through the Ral/RALBP1-dependent pathway and may be involved in suppression of activin-induced signal transduction. Isoform 2 and isoform 3 show a stimulatory affect on activin-induced signal transduction and enhance activin type 2 expression at the cell surface.
Subunit structure	Binds (via the PDZ domain) to isoform 2A of SYNJ2 (via the unique motif in the C-terminus) By similarity. Interacts (via C-terminus) with RALBP1. Interacts (via PDZ domain) with ACVR2A (via C-terminus) and ACVR2B (via C-terminus). Forms a ternary complex with ACVR2A and RALBP1.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side By similarity. Cytoplasm › perinuclear region.
Tissue specificity	Isoform 1 and isoform 2 are widely expressed, notably in brain, heart, lung, liver, kidney, skeletal muscle, ovary and testis. Isoform 3 is detected only in heart, spleen and testis.
Induction	Up-regulated between 12 and 24 hours after treatment with activin A and lipopolysaccharide (LPS). Down-regulated by calcium ionophore A23187.
Sequence similarities	Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Gene Ontology (GO)
Biological process	Rho protein signal transduction Ref.1 Inferred from direct assay. Source: MGI protein targeting Ref.1 Inferred from direct assay. Source: MGI regulation of endocytosis Ref.1 Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Lrp1	Q91ZX7	1	EBI-300910,EBI-300955

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D6K5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9D6K5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     100-118: LPVQNGPIVHRGEGEPSGV → VGITCTWIWDSRLLHCSCE
     119-145: Missing.

Isoform 3 (identifier: Q9D6K5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MNGRVDYLVTEEEINLTRGP → MIF
     100-118: LPVQNGPIVHRGEGEPSGV → VGITCTWIWDSRLLHCSCE
     119-145: Missing.

Isoform 4 (identifier: Q9D6K5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     101-145: PVQNGPIVHR...WAFVRYRKQL → LVVGGSFGLR...ERLLCLLCRQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Synaptojanin-2-binding protein		PRO_0000375982
Regions
	Topological domain	1 – 117	117	Cytoplasmic Potential
	Transmembrane	118 – 138	21	Anchor for type IV membrane protein Potential
	Topological domain	139 – 145	7	Mitochondrial intermembrane Potential
	Domain	13 – 100	88	PDZ
Natural variations
	Alternative sequence	1 – 20	20	MNGRV…LTRGP → MIF in isoform 3.		VSP_053101
	Alternative sequence	100 – 118	19	LPVQN…EPSGV → VGITCTWIWDSRLLHCSCE in isoform 2 and isoform 3.		VSP_053102
	Alternative sequence	101 – 145	45	PVQNG…YRKQL → LVVGGSFGLREFSQIRYDAV TIKIDPELEKKLKVNKITLE SEYERLLCLLCRQ in isoform 4.		VSP_053103
	Alternative sequence	119 – 145	27	Missing in isoform 2 and isoform 3.		VSP_053104
Experimental info
	Sequence conflict	34	1	Q → E in BAB25432. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BB40BB0114084F67	FASTA	145	15,815
	10 20 30 40 50 60 MNGRVDYLVT EEEINLTRGP SGLGFNIVGG TDQQYVSNDS GIYVSRIKED GAAAQDGRLQ 70 80 90 100 110 120 EGDKILSVNG QDLKNLLHQD AVDLFRNAGC AVSLRVQHRL PVQNGPIVHR GEGEPSGVPV 130 140 AMVLLPVFAL TMVAVWAFVR YRKQL
	Isoform 2. Checksum: 5450D7582F5A297C	FASTA	118	12,994
	10 20 30 40 50 60 MNGRVDYLVT EEEINLTRGP SGLGFNIVGG TDQQYVSNDS GIYVSRIKED GAAAQDGRLQ 70 80 90 100 110 EGDKILSVNG QDLKNLLHQD AVDLFRNAGC AVSLRVQHRV GITCTWIWDS RLLHCSCE
	Isoform 3. Checksum: 58A5064CBC35EC0E	FASTA	101	11,096
	10 20 30 40 50 60 MIFSGLGFNI VGGTDQQYVS NDSGIYVSRI KEDGAAAQDG RLQEGDKILS VNGQDLKNLL 70 80 90 100 HQDAVDLFRN AGCAVSLRVQ HRVGITCTWI WDSRLLHCSC E
	Isoform 4. Checksum: 86BFE35BEEAF0D9A	FASTA	153	17,040
	10 20 30 40 50 60 MNGRVDYLVT EEEINLTRGP SGLGFNIVGG TDQQYVSNDS GIYVSRIKED GAAAQDGRLQ 70 80 90 100 110 120 EGDKILSVNG QDLKNLLHQD AVDLFRNAGC AVSLRVQHRL LVVGGSFGLR EFSQIRYDAV 130 140 150 TIKIDPELEK KLKVNKITLE SEYERLLCLL CRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway." Matsuzaki T., Hanai S., Kishi H., Liu Z., Bao Y., Kikuchi A., Tsuchida K., Sugino H. J. Biol. Chem. 277:19008-19018(2002) [PubMed: 11882656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, SUBUNIT, INTERACTION WITH ACVR2A; ACVR2B AND RALBP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling." Liu Z.H., Tsuchida K., Matsuzaki T., Bao Y.L., Kurisaki A., Sugino H. J. Endocrinol. 189:409-421(2006) [PubMed: 16648306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, INTERACTION WITH ACVR2A. Strain: C57BL/6.
[3]	"Identification and characterization of a novel protein that interacts with activin type II receptors." Tai G.X., Liu Z.H., Xu G.Y., Yang Y., Zhang P.Y. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Colon, Egg, Hippocampus and Small intestine.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[6]	"Regulation of activin receptor-interacting protein 2 expression in mouse hepatoma Hepa1-6 cells and its relationship with collagen type IV." Zhang H.J., Tai G.X., Zhou J., Ma D., Liu Z.H. World J. Gastroenterol. 13:5501-5505(2007) [PubMed: 17907296] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF414433 mRNA. Translation: AAM43958.1. AY071903 mRNA. Translation: AAL60065.1. AY157057 mRNA. Translation: AAO12271.1. AY138960 mRNA. Translation: AAN17786.1. AY566156 mRNA. Translation: AAT70239.1. AK008054 mRNA. Translation: BAB25432.1. AK013474 mRNA. Translation: BAB28873.1. AK033514 mRNA. Translation: BAC28333.1. AK163362 mRNA. Translation: BAE37317.1. BC027433 mRNA. Translation: AAH27433.2. Different initiation.
IPI	IPI00109206.
RefSeq	NP_079568.1.
UniGene	Mm.279603 Mm.440213
3D structure databases
HSSP	HSSP built from PDB template 1QLC based on UniProtKB P31016.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9D6K5. 2 interactions.
Proteomic databases
PRIDE	Q9D6K5.
Genome annotation databases
Ensembl	ENSMUSG00000021139. Mus musculus. [Contig view]
GeneID	24071.
KEGG	mmu:24071.
Organism-specific databases
MGI	MGI:1344347. Synj2bp.
Phylogenomic databases
HOVERGEN	Q9D6K5.
OMA	Q9D6K5. HRSEGEP.
Gene expression databases
ArrayExpress	Q9D6K5.
Bgee	Q9D6K5.
Family and domain databases
InterPro	IPR001478. PDZ/DHR/GLGF. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
PROSITE	PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	304059.
SOURCE	Search...

Entry information

Entry name

SYJ2B_MOUSE

Accession

Primary (citable) accession number: Q9D6K5
Secondary accession number(s): Q2TTN6 , Q6YNE6, Q78HT9, Q8K4F3, Q9D8G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)