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Reviewed, UniProtKB/Swiss-Prot Q9D6K5 (SYJ2B_MOUSE)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Synaptojanin-2-binding protein
Alternative name(s):
    Activin receptor-interacting protein 2
    Activin receptor-interacting protein 4
    Mitochondrial outer membrane protein 25
Gene names
Name: Synj2bp
Synonyms: Arip2, Omp25
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length145 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Isoform 1 regulates endocytosis of activin type 2 receptor kinases through the Ral/RALBP1-dependent pathway and may be involved in suppression of activin-induced signal transduction. Isoform 2 and isoform 3 show a stimulatory affect on activin-induced signal transduction and enhance activin type 2 expression at the cell surface.

Subunit structure

Binds (via the PDZ domain) to isoform 2A of SYNJ2 (via the unique motif in the C-terminus) By similarity. Interacts (via C-terminus) with RALBP1. Interacts (via PDZ domain) with ACVR2A (via C-terminus) and ACVR2B (via C-terminus). Forms a ternary complex with ACVR2A and RALBP1.

Subcellular location

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side By similarity. Cytoplasmperinuclear region.

Tissue specificity

Isoform 1 and isoform 2 are widely expressed, notably in brain, heart, lung, liver, kidney, skeletal muscle, ovary and testis. Isoform 3 is detected only in heart, spleen and testis.

Induction

Up-regulated between 12 and 24 hours after treatment with activin A and lipopolysaccharide (LPS). Down-regulated by calcium ionophore A23187.

Sequence similarities

Contains 1 PDZ (DHR) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Lrp1Q91ZX71EBI-300910,EBI-300955

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9D6K5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9D6K5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     100-118: LPVQNGPIVHRGEGEPSGV → VGITCTWIWDSRLLHCSCE
     119-145: Missing.
Isoform 3 (identifier: Q9D6K5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MNGRVDYLVTEEEINLTRGP → MIF
     100-118: LPVQNGPIVHRGEGEPSGV → VGITCTWIWDSRLLHCSCE
     119-145: Missing.
Isoform 4 (identifier: Q9D6K5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     101-145: PVQNGPIVHR...WAFVRYRKQL → LVVGGSFGLR...ERLLCLLCRQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 145145Synaptojanin-2-binding protein
PRO_0000375982

Regions

Topological domain1 – 117117Cytoplasmic Potential
Transmembrane118 – 13821Anchor for type IV membrane protein Potential
Topological domain139 – 1457Mitochondrial intermembrane Potential
Domain13 – 10088PDZ

Natural variations

Alternative sequence1 – 2020MNGRV…LTRGP → MIF in isoform 3.
VSP_053101
Alternative sequence100 – 11819LPVQN…EPSGV → VGITCTWIWDSRLLHCSCE in isoform 2 and isoform 3.
VSP_053102
Alternative sequence101 – 14545PVQNG…YRKQL → LVVGGSFGLREFSQIRYDAV TIKIDPELEKKLKVNKITLE SEYERLLCLLCRQ in isoform 4.
VSP_053103
Alternative sequence119 – 14527Missing in isoform 2 and isoform 3.
VSP_053104

Experimental info

Sequence conflict341Q → E in BAB25432. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: BB40BB0114084F67

FASTA14515,815
        10         20         30         40         50         60 
MNGRVDYLVT EEEINLTRGP SGLGFNIVGG TDQQYVSNDS GIYVSRIKED GAAAQDGRLQ 

        70         80         90        100        110        120 
EGDKILSVNG QDLKNLLHQD AVDLFRNAGC AVSLRVQHRL PVQNGPIVHR GEGEPSGVPV 

       130        140 
AMVLLPVFAL TMVAVWAFVR YRKQL 

« Hide

Isoform 2.

Checksum: 5450D7582F5A297C
Show »

FASTA11812,994
Isoform 3.

Checksum: 58A5064CBC35EC0E
Show »

FASTA10111,096
Isoform 4.

Checksum: 86BFE35BEEAF0D9A
Show »

FASTA15317,040

References

« Hide 'large scale' references
[1]"Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway."
Matsuzaki T., Hanai S., Kishi H., Liu Z., Bao Y., Kikuchi A., Tsuchida K., Sugino H.
J. Biol. Chem. 277:19008-19018(2002) [PubMed: 11882656] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, SUBUNIT, INTERACTION WITH ACVR2A; ACVR2B AND RALBP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling."
Liu Z.H., Tsuchida K., Matsuzaki T., Bao Y.L., Kurisaki A., Sugino H.
J. Endocrinol. 189:409-421(2006) [PubMed: 16648306] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, INTERACTION WITH ACVR2A.
Strain: C57BL/6.
[3]"Identification and characterization of a novel protein that interacts with activin type II receptors."
Tai G.X., Liu Z.H., Xu G.Y., Yang Y., Zhang P.Y.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Colon, Egg, Hippocampus and Small intestine.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[6]"Regulation of activin receptor-interacting protein 2 expression in mouse hepatoma Hepa1-6 cells and its relationship with collagen type IV."
Zhang H.J., Tai G.X., Zhou J., Ma D., Liu Z.H.
World J. Gastroenterol. 13:5501-5505(2007) [PubMed: 17907296] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF414433 mRNA. Translation: AAM43958.1.
AY071903 mRNA. Translation: AAL60065.1.
AY157057 mRNA. Translation: AAO12271.1.
AY138960 mRNA. Translation: AAN17786.1.
AY566156 mRNA. Translation: AAT70239.1.
AK008054 mRNA. Translation: BAB25432.1.
AK013474 mRNA. Translation: BAB28873.1.
AK033514 mRNA. Translation: BAC28333.1.
AK163362 mRNA. Translation: BAE37317.1.
BC027433 mRNA. Translation: AAH27433.2. Different initiation.
IPIIPI00109206.
RefSeqNP_079568.1.
UniGeneMm.279603
Mm.440213

3D structure databases

HSSPHSSP built from PDB template 1QLC based on UniProtKB P31016.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9D6K5. 2 interactions.

Proteomic databases

PRIDEQ9D6K5.

Genome annotation databases

EnsemblENSMUSG00000021139. Mus musculus. [Contig view]
GeneID24071.
KEGGmmu:24071.

Organism-specific databases

MGIMGI:1344347. Synj2bp.

Phylogenomic databases

HOVERGENQ9D6K5.
OMAQ9D6K5. HRSEGEP.

Gene expression databases

ArrayExpressQ9D6K5.
BgeeQ9D6K5.

Family and domain databases

InterProIPR001478. PDZ/DHR/GLGF.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio304059.
SOURCESearch...

Entry information

Entry nameSYJ2B_MOUSE
AccessionPrimary (citable) accession number: Q9D6K5
Secondary accession number(s): Q2TTN6 expand/collapse secondary AC list , Q6YNE6, Q78HT9, Q8K4F3, Q9D8G4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2001
Last modified: June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents