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Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

Ndufv2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi139 – 1391Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi175 – 1751Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi179 – 1791Iron-sulfur (2Fe-2S)Sequence Analysis

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: GO_Central
  3. metal ion binding Source: UniProtKB-KW
  4. NADH dehydrogenase (ubiquinone) activity Source: MGI

GO - Biological processi

  1. cardiac muscle tissue development Source: MGI
  2. mitochondrial electron transport, NADH to ubiquinone Source: MGI
  3. nervous system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiREACT_262523. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH-ubiquinone oxidoreductase 24 kDa subunit
Gene namesi
Name:Ndufv2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1920150. Ndufv2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: MGI
  2. mitochondrion Source: MGI
  3. myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionAdd
BLAST
Chaini32 – 248217NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020004Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601N6-acetyllysine1 Publication
Modified residuei192 – 1921Phosphotyrosine; by SRCBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9D6J6.
PaxDbiQ9D6J6.
PRIDEiQ9D6J6.

2D gel databases

REPRODUCTION-2DPAGEQ9D6J6.

PTM databases

PhosphoSiteiQ9D6J6.

Expressioni

Gene expression databases

BgeeiQ9D6J6.
CleanExiMM_NDUFV2.
ExpressionAtlasiQ9D6J6. baseline and differential.
GenevestigatoriQ9D6J6.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

BioGridi215635. 3 interactions.
IntActiQ9D6J6. 7 interactions.
MINTiMINT-5107881.

Structurei

3D structure databases

ProteinModelPortaliQ9D6J6.
SMRiQ9D6J6. Positions 62-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1905.
GeneTreeiENSGT00390000017580.
HOVERGENiHBG029601.
InParanoidiQ9D6J6.
KOiK03943.
OMAiVHRDTPY.
OrthoDBiEOG73JKW9.
PhylomeDBiQ9D6J6.
TreeFamiTF300004.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10371. PTHR10371. 1 hit.
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6J6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSLALRARA TGLAAQWGRH ARNLHKTAVH NGAGGALFVH RDTPENNPDT
60 70 80 90 100
PFDFTPENYK RIEAIVKNYP EGHQAAAVLP VLDLAQRQNG WLPISAMNKV
110 120 130 140 150
AEVLQVPPMR VYEVATFYTM YNRKPVGKYH IQVCTTTPCM LRDSDSILET
160 170 180 190 200
LQRKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN YYEDLTPKDI
210 220 230 240
EEIIDELKAG KVPKPGPRSG RFCCEPAGGL TSLTEPPKGP GFGVQAGL
Length:248
Mass (Da):27,285
Last modified:May 1, 2007 - v2
Checksum:iDE0111BE49A1867E
GO
Isoform 2 (identifier: Q9D6J6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: Missing.

Show »
Length:152
Mass (Da):16,736
Checksum:i19813D8C2F6A63A3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641A → T in BAB28888 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9696Missing in isoform 2. 1 PublicationVSP_025017Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013511 mRNA. Translation: BAB28888.1.
AK078351 mRNA. Translation: BAC37233.1.
AK088193 mRNA. Translation: BAC40201.1.
AK146998 mRNA. Translation: BAE27595.1.
AK150404 mRNA. Translation: BAE29530.1.
AK151729 mRNA. Translation: BAE30647.1.
AK159460 mRNA. Translation: BAE35102.1.
AK166539 mRNA. Translation: BAE38841.1.
AK169143 mRNA. Translation: BAE40922.1.
BC030946 mRNA. Translation: AAH30946.1.
CCDSiCCDS37679.1. [Q9D6J6-1]
CCDS70836.1. [Q9D6J6-2]
RefSeqiNP_001265344.1. NM_001278415.1. [Q9D6J6-2]
NP_082664.1. NM_028388.3. [Q9D6J6-1]
UniGeneiMm.2206.

Genome annotation databases

EnsembliENSMUST00000024909; ENSMUSP00000024909; ENSMUSG00000024099. [Q9D6J6-2]
ENSMUST00000143987; ENSMUSP00000121557; ENSMUSG00000024099. [Q9D6J6-1]
GeneIDi72900.
KEGGimmu:72900.
UCSCiuc008dgw.1. mouse. [Q9D6J6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013511 mRNA. Translation: BAB28888.1.
AK078351 mRNA. Translation: BAC37233.1.
AK088193 mRNA. Translation: BAC40201.1.
AK146998 mRNA. Translation: BAE27595.1.
AK150404 mRNA. Translation: BAE29530.1.
AK151729 mRNA. Translation: BAE30647.1.
AK159460 mRNA. Translation: BAE35102.1.
AK166539 mRNA. Translation: BAE38841.1.
AK169143 mRNA. Translation: BAE40922.1.
BC030946 mRNA. Translation: AAH30946.1.
CCDSiCCDS37679.1. [Q9D6J6-1]
CCDS70836.1. [Q9D6J6-2]
RefSeqiNP_001265344.1. NM_001278415.1. [Q9D6J6-2]
NP_082664.1. NM_028388.3. [Q9D6J6-1]
UniGeneiMm.2206.

3D structure databases

ProteinModelPortaliQ9D6J6.
SMRiQ9D6J6. Positions 62-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215635. 3 interactions.
IntActiQ9D6J6. 7 interactions.
MINTiMINT-5107881.

PTM databases

PhosphoSiteiQ9D6J6.

2D gel databases

REPRODUCTION-2DPAGEQ9D6J6.

Proteomic databases

MaxQBiQ9D6J6.
PaxDbiQ9D6J6.
PRIDEiQ9D6J6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024909; ENSMUSP00000024909; ENSMUSG00000024099. [Q9D6J6-2]
ENSMUST00000143987; ENSMUSP00000121557; ENSMUSG00000024099. [Q9D6J6-1]
GeneIDi72900.
KEGGimmu:72900.
UCSCiuc008dgw.1. mouse. [Q9D6J6-1]

Organism-specific databases

CTDi4729.
MGIiMGI:1920150. Ndufv2.

Phylogenomic databases

eggNOGiCOG1905.
GeneTreeiENSGT00390000017580.
HOVERGENiHBG029601.
InParanoidiQ9D6J6.
KOiK03943.
OMAiVHRDTPY.
OrthoDBiEOG73JKW9.
PhylomeDBiQ9D6J6.
TreeFamiTF300004.

Enzyme and pathway databases

ReactomeiREACT_262523. Respiratory electron transport.

Miscellaneous databases

ChiTaRSiNdufv2. mouse.
NextBioi337117.
PROiQ9D6J6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6J6.
CleanExiMM_NDUFV2.
ExpressionAtlasiQ9D6J6. baseline and differential.
GenevestigatoriQ9D6J6.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10371. PTHR10371. 1 hit.
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Cerebellum, Heart, Hippocampus and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  3. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 42-61; 68-123; 129-153 AND 199-208, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNDUV2_MOUSE
AccessioniPrimary (citable) accession number: Q9D6J6
Secondary accession number(s): Q3U9L9, Q8BU07, Q8K2L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: May 1, 2007
Last modified: February 4, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.