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Q9D6J6

- NDUV2_MOUSE

UniProt

Q9D6J6 - NDUV2_MOUSE

Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

Ndufv2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi139 – 1391Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi175 – 1751Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi179 – 1791Iron-sulfur (2Fe-2S)Sequence Analysis

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: RefGenome
    3. metal ion binding Source: UniProtKB-KW
    4. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. cardiac muscle tissue development Source: Ensembl
    2. mitochondrial electron transport, NADH to ubiquinone Source: RefGenome
    3. nervous system development Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    NADH-ubiquinone oxidoreductase 24 kDa subunit
    Gene namesi
    Name:Ndufv2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1920150. Ndufv2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: RefGenome
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3131MitochondrionAdd
    BLAST
    Chaini32 – 248217NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020004Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei60 – 601N6-acetyllysine1 Publication
    Modified residuei192 – 1921Phosphotyrosine; by SRCBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9D6J6.
    PaxDbiQ9D6J6.
    PRIDEiQ9D6J6.

    2D gel databases

    REPRODUCTION-2DPAGEQ9D6J6.

    PTM databases

    PhosphoSiteiQ9D6J6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9D6J6.
    BgeeiQ9D6J6.
    CleanExiMM_NDUFV2.
    GenevestigatoriQ9D6J6.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme By similarity.By similarity

    Protein-protein interaction databases

    BioGridi215635. 3 interactions.
    IntActiQ9D6J6. 7 interactions.
    MINTiMINT-5107881.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D6J6.
    SMRiQ9D6J6. Positions 62-211.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 24 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1905.
    GeneTreeiENSGT00390000017580.
    HOVERGENiHBG029601.
    InParanoidiQ9D6J6.
    KOiK03943.
    OMAiVHRDTPY.
    OrthoDBiEOG73JKW9.
    PhylomeDBiQ9D6J6.
    TreeFamiTF300004.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR10371. PTHR10371. 1 hit.
    PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
    PROSITEiPS01099. COMPLEX1_24K. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9D6J6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFSLALRARA TGLAAQWGRH ARNLHKTAVH NGAGGALFVH RDTPENNPDT    50
    PFDFTPENYK RIEAIVKNYP EGHQAAAVLP VLDLAQRQNG WLPISAMNKV 100
    AEVLQVPPMR VYEVATFYTM YNRKPVGKYH IQVCTTTPCM LRDSDSILET 150
    LQRKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN YYEDLTPKDI 200
    EEIIDELKAG KVPKPGPRSG RFCCEPAGGL TSLTEPPKGP GFGVQAGL 248
    Length:248
    Mass (Da):27,285
    Last modified:May 1, 2007 - v2
    Checksum:iDE0111BE49A1867E
    GO
    Isoform 2 (identifier: Q9D6J6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-96: Missing.

    Show »
    Length:152
    Mass (Da):16,736
    Checksum:i19813D8C2F6A63A3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641A → T in BAB28888. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9696Missing in isoform 2. 1 PublicationVSP_025017Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013511 mRNA. Translation: BAB28888.1.
    AK078351 mRNA. Translation: BAC37233.1.
    AK088193 mRNA. Translation: BAC40201.1.
    AK146998 mRNA. Translation: BAE27595.1.
    AK150404 mRNA. Translation: BAE29530.1.
    AK151729 mRNA. Translation: BAE30647.1.
    AK159460 mRNA. Translation: BAE35102.1.
    AK166539 mRNA. Translation: BAE38841.1.
    AK169143 mRNA. Translation: BAE40922.1.
    BC030946 mRNA. Translation: AAH30946.1.
    CCDSiCCDS37679.1. [Q9D6J6-1]
    CCDS70836.1. [Q9D6J6-2]
    RefSeqiNP_001265344.1. NM_001278415.1. [Q9D6J6-2]
    NP_082664.1. NM_028388.3. [Q9D6J6-1]
    UniGeneiMm.2206.

    Genome annotation databases

    EnsembliENSMUST00000024909; ENSMUSP00000024909; ENSMUSG00000024099. [Q9D6J6-2]
    ENSMUST00000143987; ENSMUSP00000121557; ENSMUSG00000024099. [Q9D6J6-1]
    GeneIDi72900.
    KEGGimmu:72900.
    UCSCiuc008dgw.1. mouse. [Q9D6J6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013511 mRNA. Translation: BAB28888.1 .
    AK078351 mRNA. Translation: BAC37233.1 .
    AK088193 mRNA. Translation: BAC40201.1 .
    AK146998 mRNA. Translation: BAE27595.1 .
    AK150404 mRNA. Translation: BAE29530.1 .
    AK151729 mRNA. Translation: BAE30647.1 .
    AK159460 mRNA. Translation: BAE35102.1 .
    AK166539 mRNA. Translation: BAE38841.1 .
    AK169143 mRNA. Translation: BAE40922.1 .
    BC030946 mRNA. Translation: AAH30946.1 .
    CCDSi CCDS37679.1. [Q9D6J6-1 ]
    CCDS70836.1. [Q9D6J6-2 ]
    RefSeqi NP_001265344.1. NM_001278415.1. [Q9D6J6-2 ]
    NP_082664.1. NM_028388.3. [Q9D6J6-1 ]
    UniGenei Mm.2206.

    3D structure databases

    ProteinModelPortali Q9D6J6.
    SMRi Q9D6J6. Positions 62-211.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215635. 3 interactions.
    IntActi Q9D6J6. 7 interactions.
    MINTi MINT-5107881.

    PTM databases

    PhosphoSitei Q9D6J6.

    2D gel databases

    REPRODUCTION-2DPAGE Q9D6J6.

    Proteomic databases

    MaxQBi Q9D6J6.
    PaxDbi Q9D6J6.
    PRIDEi Q9D6J6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024909 ; ENSMUSP00000024909 ; ENSMUSG00000024099 . [Q9D6J6-2 ]
    ENSMUST00000143987 ; ENSMUSP00000121557 ; ENSMUSG00000024099 . [Q9D6J6-1 ]
    GeneIDi 72900.
    KEGGi mmu:72900.
    UCSCi uc008dgw.1. mouse. [Q9D6J6-1 ]

    Organism-specific databases

    CTDi 4729.
    MGIi MGI:1920150. Ndufv2.

    Phylogenomic databases

    eggNOGi COG1905.
    GeneTreei ENSGT00390000017580.
    HOVERGENi HBG029601.
    InParanoidi Q9D6J6.
    KOi K03943.
    OMAi VHRDTPY.
    OrthoDBi EOG73JKW9.
    PhylomeDBi Q9D6J6.
    TreeFami TF300004.

    Miscellaneous databases

    ChiTaRSi NDUFV2. mouse.
    NextBioi 337117.
    PROi Q9D6J6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D6J6.
    Bgeei Q9D6J6.
    CleanExi MM_NDUFV2.
    Genevestigatori Q9D6J6.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR10371. PTHR10371. 1 hit.
    PIRSFi PIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01958. nuoE_fam. 1 hit.
    PROSITEi PS01099. COMPLEX1_24K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Cerebellum, Heart, Hippocampus and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N-3.
      Tissue: Mammary tumor.
    3. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 42-61; 68-123; 129-153 AND 199-208, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiNDUV2_MOUSE
    AccessioniPrimary (citable) accession number: Q9D6J6
    Secondary accession number(s): Q3U9L9, Q8BU07, Q8K2L0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3