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Protein

N-terminal EF-hand calcium-binding protein 3

Gene

Necab3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi40 – 52PROSITE-ProRule annotationAdd BLAST13

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal EF-hand calcium-binding protein 3
Alternative name(s):
Amyloid beta A4 protein-binding family A member 2-binding protein
X11L-binding protein 51
Short name:
mXB51
Gene namesi
Name:Necab3
Synonyms:Apba2bp, Xb51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1861721. Necab3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000738641 – 353N-terminal EF-hand calcium-binding protein 3Add BLAST353

Post-translational modificationi

Phosphorylated by NEK2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D6J4.
PRIDEiQ9D6J4.

PTM databases

PhosphoSitePlusiQ9D6J4.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in the brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000027489.
CleanExiMM_NECAB3.
ExpressionAtlasiQ9D6J4. baseline and differential.
GenevisibleiQ9D6J4. MM.

Interactioni

Subunit structurei

Interacts with the N-terminal domain of APBA2. Interacts with NEK2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000895.

Structurei

3D structure databases

ProteinModelPortaliQ9D6J4.
SMRiQ9D6J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 62EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini253 – 342ABMAdd BLAST90

Sequence similaritiesi

Contains 1 ABM domain.Curated
Contains 1 EF-hand domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOVERGENiHBG050433.
InParanoidiQ9D6J4.
OMAiALCCYVD.
OrthoDBiEOG091G0DWL.
PhylomeDBiQ9D6J4.
TreeFamiTF331029.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6J4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACAGLLTVC LLGPPAPQQP RHSAPAAGHA LFQDVFRRAD KNDDGKLSFE
60 70 80 90 100
EFQNYFADGV LSSAELRELF SGIDDHLTDN LETEKLCDYF STHLGVYRPV
110 120 130 140 150
LAALESLNRA VLTAMDTTKL EYEQASKVDQ FVTRFLLRET VNQLQALQTS
160 170 180 190 200
LEGASDTLEA QAHGQRLDEE TIKAQSRPCG SRRAGRRALR SISWSPSWSP
210 220 230 240 250
GSSDTGRSSE AEQQWRLQVN RLQELIDQLE CKAPRLEPTH EEDLTKGFDS
260 270 280 290 300
HILVAQRQVQ VAEDALQDFH RALCCYMNFT GAQSHCLHVS AQKMLDNAAF
310 320 330 340 350
TLYEFWQDEA SWRRHQQSPC SKAFQRTLID HLQAPDTLTT VFFPASWWIM

NNN
Length:353
Mass (Da):39,906
Last modified:February 28, 2003 - v2
Checksum:i606C1D570FA36864
GO
Isoform 2 (identifier: Q9D6J4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-256: Missing.

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):37,220
Checksum:i895FDAC84109E26E
GO

Sequence cautioni

The sequence AAI26875 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI26876 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB16414 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166R → W in AAI26875 (PubMed:15489334).Curated1
Sequence conflicti305F → C in BAB28895 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000740233 – 256Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013520 mRNA. Translation: BAB28895.1.
AK044296 mRNA. Translation: BAC31859.1.
AK158225 mRNA. Translation: BAE34411.1.
AL772292 Genomic DNA. Translation: CAM13562.1.
AL772292 Genomic DNA. Translation: CAM13563.1.
CH466551 Genomic DNA. Translation: EDL06077.1.
CH466551 Genomic DNA. Translation: EDL06081.1.
AB039948 mRNA. Translation: BAB16414.1. Different initiation.
BC126874 mRNA. Translation: AAI26875.1. Different initiation.
BC126875 mRNA. Translation: AAI26876.1. Different initiation.
CCDSiCCDS50766.1. [Q9D6J4-1]
RefSeqiNP_067521.2. NM_021546.3. [Q9D6J4-1]
UniGeneiMm.143748.

Genome annotation databases

EnsembliENSMUST00000000895; ENSMUSP00000000895; ENSMUSG00000027489. [Q9D6J4-1]
ENSMUST00000109716; ENSMUSP00000105338; ENSMUSG00000027489. [Q9D6J4-2]
GeneIDi56846.
KEGGimmu:56846.
UCSCiuc008njh.1. mouse. [Q9D6J4-1]
uc008nji.1. mouse. [Q9D6J4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013520 mRNA. Translation: BAB28895.1.
AK044296 mRNA. Translation: BAC31859.1.
AK158225 mRNA. Translation: BAE34411.1.
AL772292 Genomic DNA. Translation: CAM13562.1.
AL772292 Genomic DNA. Translation: CAM13563.1.
CH466551 Genomic DNA. Translation: EDL06077.1.
CH466551 Genomic DNA. Translation: EDL06081.1.
AB039948 mRNA. Translation: BAB16414.1. Different initiation.
BC126874 mRNA. Translation: AAI26875.1. Different initiation.
BC126875 mRNA. Translation: AAI26876.1. Different initiation.
CCDSiCCDS50766.1. [Q9D6J4-1]
RefSeqiNP_067521.2. NM_021546.3. [Q9D6J4-1]
UniGeneiMm.143748.

3D structure databases

ProteinModelPortaliQ9D6J4.
SMRiQ9D6J4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000895.

PTM databases

PhosphoSitePlusiQ9D6J4.

Proteomic databases

PaxDbiQ9D6J4.
PRIDEiQ9D6J4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000895; ENSMUSP00000000895; ENSMUSG00000027489. [Q9D6J4-1]
ENSMUST00000109716; ENSMUSP00000105338; ENSMUSG00000027489. [Q9D6J4-2]
GeneIDi56846.
KEGGimmu:56846.
UCSCiuc008njh.1. mouse. [Q9D6J4-1]
uc008nji.1. mouse. [Q9D6J4-2]

Organism-specific databases

CTDi63941.
MGIiMGI:1861721. Necab3.

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOVERGENiHBG050433.
InParanoidiQ9D6J4.
OMAiALCCYVD.
OrthoDBiEOG091G0DWL.
PhylomeDBiQ9D6J4.
TreeFamiTF331029.

Miscellaneous databases

PROiQ9D6J4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027489.
CleanExiMM_NECAB3.
ExpressionAtlasiQ9D6J4. baseline and differential.
GenevisibleiQ9D6J4. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNECA3_MOUSE
AccessioniPrimary (citable) accession number: Q9D6J4
Secondary accession number(s): A1L0S0
, A1L0S1, A2AKE8, A2AKE9, Q3TYZ9, Q9ESR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.