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Protein

N-terminal EF-hand calcium-binding protein 3

Gene

Necab3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi40 – 5213PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal EF-hand calcium-binding protein 3
Alternative name(s):
Amyloid beta A4 protein-binding family A member 2-binding protein
X11L-binding protein 51
Short name:
mXB51
Gene namesi
Name:Necab3
Synonyms:Apba2bp, Xb51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1861721. Necab3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353N-terminal EF-hand calcium-binding protein 3PRO_0000073864Add
BLAST

Post-translational modificationi

Phosphorylated by NEK2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9D6J4.
PaxDbiQ9D6J4.
PRIDEiQ9D6J4.

PTM databases

PhosphoSiteiQ9D6J4.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in the brain.1 Publication

Gene expression databases

BgeeiQ9D6J4.
CleanExiMM_NECAB3.
ExpressionAtlasiQ9D6J4. baseline and differential.
GenevisibleiQ9D6J4. MM.

Interactioni

Subunit structurei

Interacts with the N-terminal domain of APBA2. Interacts with NEK2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000895.

Structurei

3D structure databases

ProteinModelPortaliQ9D6J4.
SMRiQ9D6J4. Positions 32-87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6236EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini253 – 34290ABMAdd
BLAST

Sequence similaritiesi

Contains 1 ABM domain.Curated
Contains 1 EF-hand domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOVERGENiHBG050433.
InParanoidiQ9D6J4.
OMAiHRALCCY.
OrthoDBiEOG7BS49N.
PhylomeDBiQ9D6J4.
TreeFamiTF331029.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6J4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACAGLLTVC LLGPPAPQQP RHSAPAAGHA LFQDVFRRAD KNDDGKLSFE
60 70 80 90 100
EFQNYFADGV LSSAELRELF SGIDDHLTDN LETEKLCDYF STHLGVYRPV
110 120 130 140 150
LAALESLNRA VLTAMDTTKL EYEQASKVDQ FVTRFLLRET VNQLQALQTS
160 170 180 190 200
LEGASDTLEA QAHGQRLDEE TIKAQSRPCG SRRAGRRALR SISWSPSWSP
210 220 230 240 250
GSSDTGRSSE AEQQWRLQVN RLQELIDQLE CKAPRLEPTH EEDLTKGFDS
260 270 280 290 300
HILVAQRQVQ VAEDALQDFH RALCCYMNFT GAQSHCLHVS AQKMLDNAAF
310 320 330 340 350
TLYEFWQDEA SWRRHQQSPC SKAFQRTLID HLQAPDTLTT VFFPASWWIM

NNN
Length:353
Mass (Da):39,906
Last modified:February 28, 2003 - v2
Checksum:i606C1D570FA36864
GO
Isoform 2 (identifier: Q9D6J4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-256: Missing.

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):37,220
Checksum:i895FDAC84109E26E
GO

Sequence cautioni

The sequence AAI26875.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI26876.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB16414.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661R → W in AAI26875 (PubMed:15489334).Curated
Sequence conflicti305 – 3051F → C in BAB28895 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei233 – 25624Missing in isoform 2. 1 PublicationVSP_000740Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013520 mRNA. Translation: BAB28895.1.
AK044296 mRNA. Translation: BAC31859.1.
AK158225 mRNA. Translation: BAE34411.1.
AL772292 Genomic DNA. Translation: CAM13562.1.
AL772292 Genomic DNA. Translation: CAM13563.1.
CH466551 Genomic DNA. Translation: EDL06077.1.
CH466551 Genomic DNA. Translation: EDL06081.1.
AB039948 mRNA. Translation: BAB16414.1. Different initiation.
BC126874 mRNA. Translation: AAI26875.1. Different initiation.
BC126875 mRNA. Translation: AAI26876.1. Different initiation.
CCDSiCCDS50766.1. [Q9D6J4-1]
RefSeqiNP_067521.2. NM_021546.3. [Q9D6J4-1]
UniGeneiMm.143748.

Genome annotation databases

EnsembliENSMUST00000000895; ENSMUSP00000000895; ENSMUSG00000027489. [Q9D6J4-1]
ENSMUST00000109716; ENSMUSP00000105338; ENSMUSG00000027489. [Q9D6J4-2]
GeneIDi56846.
KEGGimmu:56846.
UCSCiuc008njh.1. mouse. [Q9D6J4-1]
uc008nji.1. mouse. [Q9D6J4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013520 mRNA. Translation: BAB28895.1.
AK044296 mRNA. Translation: BAC31859.1.
AK158225 mRNA. Translation: BAE34411.1.
AL772292 Genomic DNA. Translation: CAM13562.1.
AL772292 Genomic DNA. Translation: CAM13563.1.
CH466551 Genomic DNA. Translation: EDL06077.1.
CH466551 Genomic DNA. Translation: EDL06081.1.
AB039948 mRNA. Translation: BAB16414.1. Different initiation.
BC126874 mRNA. Translation: AAI26875.1. Different initiation.
BC126875 mRNA. Translation: AAI26876.1. Different initiation.
CCDSiCCDS50766.1. [Q9D6J4-1]
RefSeqiNP_067521.2. NM_021546.3. [Q9D6J4-1]
UniGeneiMm.143748.

3D structure databases

ProteinModelPortaliQ9D6J4.
SMRiQ9D6J4. Positions 32-87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000895.

PTM databases

PhosphoSiteiQ9D6J4.

Proteomic databases

MaxQBiQ9D6J4.
PaxDbiQ9D6J4.
PRIDEiQ9D6J4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000895; ENSMUSP00000000895; ENSMUSG00000027489. [Q9D6J4-1]
ENSMUST00000109716; ENSMUSP00000105338; ENSMUSG00000027489. [Q9D6J4-2]
GeneIDi56846.
KEGGimmu:56846.
UCSCiuc008njh.1. mouse. [Q9D6J4-1]
uc008nji.1. mouse. [Q9D6J4-2]

Organism-specific databases

CTDi63941.
MGIiMGI:1861721. Necab3.

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOVERGENiHBG050433.
InParanoidiQ9D6J4.
OMAiHRALCCY.
OrthoDBiEOG7BS49N.
PhylomeDBiQ9D6J4.
TreeFamiTF331029.

Miscellaneous databases

NextBioi313401.
PROiQ9D6J4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6J4.
CleanExiMM_NECAB3.
ExpressionAtlasiQ9D6J4. baseline and differential.
GenevisibleiQ9D6J4. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Hippocampus, Inner ear and Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Regulation of X11L-dependent amyloid precursor protein metabolism by XB51, a novel X11L-binding protein."
    Lee D.-S., Tomita S., Kirino Y., Suzuki T.
    J. Biol. Chem. 275:23134-23138(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-353 (ISOFORM 1).
    Tissue: Brain.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-353 (ISOFORM 1).
  6. "NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi."
    Yoo J.C., Chang J.R., Kim S.H., Jang S.K., Wolgemuth D.J., Kim K., Rhee K.
    Exp. Cell Res. 292:393-402(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiNECA3_MOUSE
AccessioniPrimary (citable) accession number: Q9D6J4
Secondary accession number(s): A1L0S0
, A1L0S1, A2AKE8, A2AKE9, Q3TYZ9, Q9ESR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: May 11, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.