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Protein

N-terminal EF-hand calcium-binding protein 3

Gene

Necab3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta (By similarity). May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may implicate the stabilization of the interaction between HIF1AN and APBA3 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi40 – 52PROSITE-ProRule annotationAdd BLAST13

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal EF-hand calcium-binding protein 3
Alternative name(s):
Amyloid-beta A4 protein-binding family A member 2-binding protein
X11L-binding protein 51
Short name:
mXB51
Gene namesi
Name:Necab3
Synonyms:Apba2bp, Xb51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1861721. Necab3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000738641 – 353N-terminal EF-hand calcium-binding protein 3Add BLAST353

Post-translational modificationi

Phosphorylated by NEK2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D6J4.
PRIDEiQ9D6J4.

PTM databases

PhosphoSitePlusiQ9D6J4.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in the brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000027489.
CleanExiMM_NECAB3.
ExpressionAtlasiQ9D6J4. baseline and differential.
GenevisibleiQ9D6J4. MM.

Interactioni

Subunit structurei

Interacts with the N-terminal domain of APBA2. Interacts with NEK2 (By similarity). Interacts with APBA3; APBA3 seems to mediate the interaction between NECAB3 and HIF1AN (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000895.

Structurei

3D structure databases

ProteinModelPortaliQ9D6J4.
SMRiQ9D6J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 62EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini253 – 342ABMAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 181Required for interaction with APBA3By similarity10

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOVERGENiHBG050433.
InParanoidiQ9D6J4.
OMAiALCCYVD.
OrthoDBiEOG091G0DWL.
PhylomeDBiQ9D6J4.
TreeFamiTF331029.

Family and domain databases

InterProiView protein in InterPro
IPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiView protein in PROSITE
PS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D6J4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACAGLLTVC LLGPPAPQQP RHSAPAAGHA LFQDVFRRAD KNDDGKLSFE
60 70 80 90 100
EFQNYFADGV LSSAELRELF SGIDDHLTDN LETEKLCDYF STHLGVYRPV
110 120 130 140 150
LAALESLNRA VLTAMDTTKL EYEQASKVDQ FVTRFLLRET VNQLQALQTS
160 170 180 190 200
LEGASDTLEA QAHGQRLDEE TIKAQSRPCG SRRAGRRALR SISWSPSWSP
210 220 230 240 250
GSSDTGRSSE AEQQWRLQVN RLQELIDQLE CKAPRLEPTH EEDLTKGFDS
260 270 280 290 300
HILVAQRQVQ VAEDALQDFH RALCCYMNFT GAQSHCLHVS AQKMLDNAAF
310 320 330 340 350
TLYEFWQDEA SWRRHQQSPC SKAFQRTLID HLQAPDTLTT VFFPASWWIM

NNN
Length:353
Mass (Da):39,906
Last modified:February 28, 2003 - v2
Checksum:i606C1D570FA36864
GO
Isoform 2 (identifier: Q9D6J4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-256: Missing.

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):37,220
Checksum:i895FDAC84109E26E
GO

Sequence cautioni

Q9D6J4: The sequence AAI26875 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
Q9D6J4: The sequence AAI26876 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
Q9D6J4: The sequence BAB16414 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166R → W in AAI26875 (PubMed:15489334).Curated1
Sequence conflicti305F → C in BAB28895 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000740233 – 256Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013520 mRNA. Translation: BAB28895.1.
AK044296 mRNA. Translation: BAC31859.1.
AK158225 mRNA. Translation: BAE34411.1.
AL772292 Genomic DNA. Translation: CAM13562.1.
AL772292 Genomic DNA. Translation: CAM13563.1.
CH466551 Genomic DNA. Translation: EDL06077.1.
CH466551 Genomic DNA. Translation: EDL06081.1.
AB039948 mRNA. Translation: BAB16414.1. Different initiation.
BC126874 mRNA. Translation: AAI26875.1. Different initiation.
BC126875 mRNA. Translation: AAI26876.1. Different initiation.
CCDSiCCDS50766.1. [Q9D6J4-1]
RefSeqiNP_067521.2. NM_021546.3. [Q9D6J4-1]
UniGeneiMm.143748.

Genome annotation databases

EnsembliENSMUST00000000895; ENSMUSP00000000895; ENSMUSG00000027489. [Q9D6J4-1]
ENSMUST00000109716; ENSMUSP00000105338; ENSMUSG00000027489. [Q9D6J4-2]
GeneIDi56846.
KEGGimmu:56846.
UCSCiuc008njh.1. mouse. [Q9D6J4-1]
uc008nji.1. mouse. [Q9D6J4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNECA3_MOUSE
AccessioniPrimary (citable) accession number: Q9D6J4
Secondary accession number(s): A1L0S0
, A1L0S1, A2AKE8, A2AKE9, Q3TYZ9, Q9ESR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: September 27, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot