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Protein

Ceramide synthase 4

Gene

Cers4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi67 – 12862HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • sphingosine N-acyltransferase activity Source: MGI

GO - Biological processi

  • ceramide biosynthetic process Source: MGI
  • sphingolipid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BRENDAi2.3.1.24. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Chemistry

SwissLipidsiSLP:000000117.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide synthase 4
Short name:
CerS4
Alternative name(s):
LAG1 longevity assurance homolog 4
Translocating chain-associating membrane protein homolog 1
Short name:
TRAM homolog 1
Gene namesi
Name:Cers4
Synonyms:Lass4, Trh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1914510. Cers4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence analysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence analysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence analysisAdd
BLAST
Transmembranei304 – 32421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Ceramide synthase 4PRO_0000185513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei342 – 3421PhosphoserineCombined sources
Modified residuei349 – 3491PhosphoserineCombined sources
Modified residuei350 – 3501PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D6J1.
MaxQBiQ9D6J1.
PaxDbiQ9D6J1.
PRIDEiQ9D6J1.

PTM databases

PhosphoSiteiQ9D6J1.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in skin.2 Publications

Gene expression databases

BgeeiQ9D6J1.
CleanExiMM_LASS4.
ExpressionAtlasiQ9D6J1. baseline and differential.
GenevisibleiQ9D6J1. MM.

Interactioni

Protein-protein interaction databases

BioGridi212053. 1 interaction.
STRINGi10090.ENSMUSP00000008350.

Structurei

3D structure databases

ProteinModelPortaliQ9D6J1.
SMRiQ9D6J1. Positions 79-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 332202TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1607. Eukaryota.
COG5058. LUCA.
GeneTreeiENSGT00550000074401.
HOVERGENiHBG052310.
InParanoidiQ9D6J1.
KOiK04710.
OMAiFNGLLMM.
PhylomeDBiQ9D6J1.
TreeFamiTF314319.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D6J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSLSEWLW QETYWLPPNV TWAELEDRDG LVFAHPHHVL AAFPVALVLV
60 70 80 90 100
AVRIVFERFV ALPLSRWMGV QDPIRRKIKP NPVLEKYFLR MKQCPEETQM
110 120 130 140 150
VLLASQCGLT LRQTQRWFRR RRNQDRPSLS KKFCEACWRF VFYLCSFVGG
160 170 180 190 200
TSILYHESWL WSPSLCWENY PHQTLNLSLY WWYLLELGFY LSLLITLPFD
210 220 230 240 250
VKRKDFKEQV VHHFVAVGLI GFSYSVNLLR IGAVVLLLHD CSDYLLEGCK
260 270 280 290 300
ILNYAHFRRG CDALFIMFAL VFFYTRLIFF PTQVIYTSVY DSIKNSGPFF
310 320 330 340 350
GYYFFIVLLV MLQILHVYWF CLILRMLYSF LHKGQMTEDI RSDVEEPDSS
360 370 380 390
DDEPVSEGPQ LKNGMARGSR VAVTNGPRSR AAACLTNGHT RAT
Length:393
Mass (Da):46,017
Last modified:June 1, 2001 - v1
Checksum:iB2038F7A128F816F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191N → S in BAC26208 (PubMed:16141072).Curated
Sequence conflicti237 – 2371L → M in BAB32370 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029531 mRNA. Translation: AAK40299.1.
AK013554 mRNA. Translation: BAB28903.1.
AK021316 mRNA. Translation: BAB32370.3.
AK028937 mRNA. Translation: BAC26202.1.
AK028945 mRNA. Translation: BAC26208.1.
AK030280 mRNA. Translation: BAC26876.1.
BC003946 mRNA. Translation: AAH03946.1.
CCDSiCCDS22086.1.
RefSeqiNP_080334.3. NM_026058.4.
XP_006508924.1. XM_006508861.2.
XP_006508925.1. XM_006508862.2.
XP_006508926.1. XM_006508863.2.
UniGeneiMm.35511.

Genome annotation databases

EnsembliENSMUST00000008350; ENSMUSP00000008350; ENSMUSG00000008206.
GeneIDi67260.
KEGGimmu:67260.
UCSCiuc009ktx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029531 mRNA. Translation: AAK40299.1.
AK013554 mRNA. Translation: BAB28903.1.
AK021316 mRNA. Translation: BAB32370.3.
AK028937 mRNA. Translation: BAC26202.1.
AK028945 mRNA. Translation: BAC26208.1.
AK030280 mRNA. Translation: BAC26876.1.
BC003946 mRNA. Translation: AAH03946.1.
CCDSiCCDS22086.1.
RefSeqiNP_080334.3. NM_026058.4.
XP_006508924.1. XM_006508861.2.
XP_006508925.1. XM_006508862.2.
XP_006508926.1. XM_006508863.2.
UniGeneiMm.35511.

3D structure databases

ProteinModelPortaliQ9D6J1.
SMRiQ9D6J1. Positions 79-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212053. 1 interaction.
STRINGi10090.ENSMUSP00000008350.

Chemistry

SwissLipidsiSLP:000000117.

PTM databases

PhosphoSiteiQ9D6J1.

Proteomic databases

EPDiQ9D6J1.
MaxQBiQ9D6J1.
PaxDbiQ9D6J1.
PRIDEiQ9D6J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008350; ENSMUSP00000008350; ENSMUSG00000008206.
GeneIDi67260.
KEGGimmu:67260.
UCSCiuc009ktx.2. mouse.

Organism-specific databases

CTDi79603.
MGIiMGI:1914510. Cers4.

Phylogenomic databases

eggNOGiKOG1607. Eukaryota.
COG5058. LUCA.
GeneTreeiENSGT00550000074401.
HOVERGENiHBG052310.
InParanoidiQ9D6J1.
KOiK04710.
OMAiFNGLLMM.
PhylomeDBiQ9D6J1.
TreeFamiTF314319.

Enzyme and pathway databases

BRENDAi2.3.1.24. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

NextBioi324028.
PROiQ9D6J1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D6J1.
CleanExiMM_LASS4.
ExpressionAtlasiQ9D6J1. baseline and differential.
GenevisibleiQ9D6J1. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Hartmann E.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hippocampus, Skin and Stomach.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. "Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors."
    Riebeling C., Allegood J.C., Wang E., Merrill A.H. Jr., Futerman A.H.
    J. Biol. Chem. 278:43452-43459(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CERAMIDE SYNTHESIS, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  5. "Mammalian Lass6 and its related family members regulate synthesis of specific ceramides."
    Mizutani Y., Kihara A., Igarashi Y.
    Biochem. J. 390:263-271(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342; SER-349 AND SER-350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiCERS4_MOUSE
AccessioniPrimary (citable) accession number: Q9D6J1
Secondary accession number(s): Q8BZA6, Q8C151, Q9CX09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.