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Protein

Ras and Rab interactor 2

Gene

Rin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ras effector protein. May function as an upstream activator and/or downstream effector for RAB5B in endocytic pathway. May function as a guanine nucleotide exchange (GEF) of RAB5B, required for activating the RAB5 proteins by exchanging bound GDP for free GTP (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Ras and Rab interactor 2
Alternative name(s):
Ras interaction/interference protein 2
Gene namesi
Name:Rin2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1921280. Rin2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 903903Ras and Rab interactor 2PRO_0000191321Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei366 – 3661PhosphoserineCombined sources
Modified residuei510 – 5101PhosphoserineCombined sources
Modified residuei518 – 5181PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9D684.
PaxDbiQ9D684.
PRIDEiQ9D684.

PTM databases

iPTMnetiQ9D684.
PhosphoSiteiQ9D684.

Expressioni

Gene expression databases

BgeeiQ9D684.
CleanExiMM_RIN2.
ExpressionAtlasiQ9D684. baseline and differential.
GenevisibleiQ9D684. MM.

Interactioni

Subunit structurei

Homotetramer; probably composed of anti-parallel linkage of two parallel dimers. Interacts with Ras. Interacts with RAB5B, with a much higher affinity for GTP-bound activated RAB5B. Does not interact with other members of the Rab family (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092053.

Structurei

3D structure databases

ProteinModelPortaliQ9D684.
SMRiQ9D684. Positions 586-759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 19094SH2PROSITE-ProRule annotationAdd
BLAST
Domaini627 – 766140VPS9PROSITE-ProRule annotationAdd
BLAST
Domaini796 – 88792Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni506 – 775270Interaction with RAB5BBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi307 – 3148Poly-Pro

Sequence similaritiesi

Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 VPS9 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG2320. Eukaryota.
ENOG410ZZW5. LUCA.
GeneTreeiENSGT00530000063053.
HOGENOMiHOG000022588.
HOVERGENiHBG023719.
InParanoidiQ9D684.
OMAiESRPPCH.
OrthoDBiEOG7PZRWP.
TreeFamiTF331067.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
IPR003123. VPS9.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF02204. VPS9. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
PS51205. VPS9. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D684-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAWTMGAQG LDKRGSFFKL IDTIASEIGE LKREMVQTDI SRENGLEPSE
60 70 80 90 100
THSMVRHKDG GYSEDKDGKT CPRDSGYDSL SNRLSILDRL LHTHPIWLQL
110 120 130 140 150
SLSEEEAAEV LQAQPPGIFL VRKSSKMQKK VLSLRLPCEF GAPLKEFTIK
160 170 180 190 200
ESTYTFSLEG SGISFADLFR LIAFYCISRD VLPFTLKLPY AISTAKTESQ
210 220 230 240 250
LEELAQLGLN FWSSSADNKP LNSPPPHRPL PSAGICPASL RQLCLINGVH
260 270 280 290 300
SIKTRTPSEL ECSQTNGALC FINPLFLKVH SQDLSTGPKR PSTRTPNANG
310 320 330 340 350
TERPRSPPPR PPPPAINSLH TSPGLSRTEP QTSMPETVNH SKHGNVALLG
360 370 380 390 400
TKPTPIPPPR LKKQASFLEA ESSAKTLTAR RPSRRSEPEP ELELELEMGT
410 420 430 440 450
AGHAGGAPPR DAPGDCTRAP PPGSESQPPP CHGARQRLSD MSLSTSSSDS
460 470 480 490 500
LEFDRSMPLY GYEADTTSSL EDYEGESDQE TMAPPIKSKK KRNSSFVLPK
510 520 530 540 550
LVKSQLRKMS GVFSSFMTPE KRMVRRIAEL SRDKCTYFGC LVQDYVSFLK
560 570 580 590 600
ENKECHVSST DMLQTIRQFM TQVKNYLSQS SELDPPIESL IPEDQIDVVL
610 620 630 640 650
EKAMHKCILK PLKGHVEAML KDFHTADGSW KQLKENLQLV RQRNPQELGV
660 670 680 690 700
FAPTPDLMEL EKIKLKFMTM QKMYSPEKKV MLLLRVCKLI YTVMENNSGR
710 720 730 740 750
MYGADDFLPV LTYVIAQCDM LELDTEIEYM MELLDPSLLH GEGGYYLTSA
760 770 780 790 800
YGALSLIKNF QEEQAARLLS SEARDTLRQW HKRRTTNRTI PSVDDFQNYL
810 820 830 840 850
RVAFQEVNSG CTGKTLLVRP YITTEDVCQL CAEKFKVEDP EEYSLFLFVD
860 870 880 890 900
ETWQQLAEDT YPQKIKAELH SRPQPHIFHF VYKRIKSDPY GVIFQNGEDL

TPS
Note: Derived from EST data.
Length:903
Mass (Da):101,559
Last modified:July 27, 2011 - v3
Checksum:iA79017A8B4E7949B
GO
Isoform 2 (identifier: Q9D684-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:850
Mass (Da):95,677
Checksum:i1830BA07DDCEE5AD
GO

Sequence cautioni

The sequence AAH05529.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH40390.3 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAB29425.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC30697.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE29760.1 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAE30311.1 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAE31576.1 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801D → G in BAE29760 (PubMed:16141072).Curated
Sequence conflicti204 – 2041L → I in BAE29760 (PubMed:16141072).Curated
Sequence conflicti204 – 2041L → I in BAE31576 (PubMed:16141072).Curated
Sequence conflicti221 – 2211L → P in AAH40390 (PubMed:15489334).Curated
Sequence conflicti385 – 3851R → S in AAH40390 (PubMed:15489334).Curated
Sequence conflicti448 – 4481S → Y in BAB29425 (PubMed:16141072).Curated
Sequence conflicti491 – 4911K → R in BAB29425 (PubMed:16141072).Curated
Sequence conflicti694 – 6941M → V in BAE30311 (PubMed:16141072).Curated
Sequence conflicti729 – 7291Y → C in BAE29760 (PubMed:16141072).Curated
Sequence conflicti748 – 7481T → A in BAE30311 (PubMed:16141072).Curated
Sequence conflicti801 – 8011R → Q in BAC30697 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5353Missing in isoform 2. 2 PublicationsVSP_007581Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014548 mRNA. Translation: BAB29425.1. Different initiation.
AK040763 mRNA. Translation: BAC30697.1. Different initiation.
AK150680 mRNA. Translation: BAE29760.1. Sequence problems.
AK151332 mRNA. Translation: BAE30311.1. Sequence problems.
AK152893 mRNA. Translation: BAE31576.1. Sequence problems.
AL672100 Genomic DNA. Translation: CAM14337.1.
BC005529 mRNA. Translation: AAH05529.1. Different initiation.
BC040390 mRNA. Translation: AAH40390.3. Sequence problems.
BI660407 mRNA. No translation available.
RefSeqiNP_083000.5. NM_028724.4.
XP_006500332.1. XM_006500269.1. [Q9D684-1]
XP_006500333.1. XM_006500270.2. [Q9D684-1]
XP_006500334.1. XM_006500271.2. [Q9D684-1]
XP_011238108.1. XM_011239806.1. [Q9D684-2]
UniGeneiMm.228799.

Genome annotation databases

EnsembliENSMUST00000110005; ENSMUSP00000105632; ENSMUSG00000001768. [Q9D684-1]
GeneIDi74030.
KEGGimmu:74030.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014548 mRNA. Translation: BAB29425.1. Different initiation.
AK040763 mRNA. Translation: BAC30697.1. Different initiation.
AK150680 mRNA. Translation: BAE29760.1. Sequence problems.
AK151332 mRNA. Translation: BAE30311.1. Sequence problems.
AK152893 mRNA. Translation: BAE31576.1. Sequence problems.
AL672100 Genomic DNA. Translation: CAM14337.1.
BC005529 mRNA. Translation: AAH05529.1. Different initiation.
BC040390 mRNA. Translation: AAH40390.3. Sequence problems.
BI660407 mRNA. No translation available.
RefSeqiNP_083000.5. NM_028724.4.
XP_006500332.1. XM_006500269.1. [Q9D684-1]
XP_006500333.1. XM_006500270.2. [Q9D684-1]
XP_006500334.1. XM_006500271.2. [Q9D684-1]
XP_011238108.1. XM_011239806.1. [Q9D684-2]
UniGeneiMm.228799.

3D structure databases

ProteinModelPortaliQ9D684.
SMRiQ9D684. Positions 586-759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092053.

PTM databases

iPTMnetiQ9D684.
PhosphoSiteiQ9D684.

Proteomic databases

MaxQBiQ9D684.
PaxDbiQ9D684.
PRIDEiQ9D684.

Protocols and materials databases

DNASUi74030.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110005; ENSMUSP00000105632; ENSMUSG00000001768. [Q9D684-1]
GeneIDi74030.
KEGGimmu:74030.

Organism-specific databases

CTDi54453.
MGIiMGI:1921280. Rin2.

Phylogenomic databases

eggNOGiKOG2320. Eukaryota.
ENOG410ZZW5. LUCA.
GeneTreeiENSGT00530000063053.
HOGENOMiHOG000022588.
HOVERGENiHBG023719.
InParanoidiQ9D684.
OMAiESRPPCH.
OrthoDBiEOG7PZRWP.
TreeFamiTF331067.

Miscellaneous databases

ChiTaRSiRin2. mouse.
NextBioi339580.
PROiQ9D684.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D684.
CleanExiMM_RIN2.
ExpressionAtlasiQ9D684. baseline and differential.
GenevisibleiQ9D684. MM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
IPR003123. VPS9.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF02204. VPS9. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
PS51205. VPS9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 427-903 (ISOFORMS 1/2).
    Strain: C57BL/6J.
    Tissue: Bone marrow macrophage and Skin.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: Czech II and FVB/N.
    Tissue: Mammary gland.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-510, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney, Lung and Spleen.

Entry informationi

Entry nameiRIN2_MOUSE
AccessioniPrimary (citable) accession number: Q9D684
Secondary accession number(s): A8Y5L0
, Q3U6Z8, Q3UAK3, Q3UC54, Q99K06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: July 27, 2011
Last modified: January 20, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.