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Protein

Ras and Rab interactor 2

Gene

Rin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ras effector protein. May function as an upstream activator and/or downstream effector for RAB5B in endocytic pathway. May function as a guanine nucleotide exchange (GEF) of RAB5B, required for activating the RAB5 proteins by exchanging bound GDP for free GTP (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras and Rab interactor 2
Alternative name(s):
Ras interaction/interference protein 2
Gene namesi
Name:Rin2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1921280 Rin2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913211 – 903Ras and Rab interactor 2Add BLAST903

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei366PhosphoserineCombined sources1
Modified residuei510PhosphoserineCombined sources1
Modified residuei518PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D684
PRIDEiQ9D684

PTM databases

iPTMnetiQ9D684
PhosphoSitePlusiQ9D684

Expressioni

Gene expression databases

BgeeiENSMUSG00000001768
CleanExiMM_RIN2
ExpressionAtlasiQ9D684 baseline and differential
GenevisibleiQ9D684 MM

Interactioni

Subunit structurei

Homotetramer; probably composed of anti-parallel linkage of two parallel dimers. Interacts with Ras. Interacts with RAB5B, with a much higher affinity for GTP-bound activated RAB5B. Does not interact with other members of the Rab family (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092053

Structurei

3D structure databases

ProteinModelPortaliQ9D684
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 190SH2PROSITE-ProRule annotationAdd BLAST94
Domaini627 – 766VPS9PROSITE-ProRule annotationAdd BLAST140
Domaini796 – 887Ras-associatingPROSITE-ProRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni506 – 775Interaction with RAB5BBy similarityAdd BLAST270

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi307 – 314Poly-Pro8

Sequence similaritiesi

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG2320 Eukaryota
ENOG410ZZW5 LUCA
GeneTreeiENSGT00530000063053
HOGENOMiHOG000022588
HOVERGENiHBG023719
InParanoidiQ9D684
OMAiRTPNANG
OrthoDBiEOG091G02V2
TreeFamiTF331067

Family and domain databases

CDDicd10394 SH2_RIN2, 1 hit
Gene3Di1.20.1050.80, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000159 RA_dom
IPR035868 RIN2_SH2
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR029071 Ubiquitin-like_domsf
IPR003123 VPS9
IPR037191 VPS9_dom_sf
PfamiView protein in Pfam
PF00788 RA, 1 hit
PF02204 VPS9, 1 hit
SMARTiView protein in SMART
SM00314 RA, 1 hit
SM00252 SH2, 1 hit
SM00167 VPS9, 1 hit
SUPFAMiSSF109993 SSF109993, 1 hit
SSF54236 SSF54236, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50001 SH2, 1 hit
PS51205 VPS9, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D684-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAWTMGAQG LDKRGSFFKL IDTIASEIGE LKREMVQTDI SRENGLEPSE
60 70 80 90 100
THSMVRHKDG GYSEDKDGKT CPRDSGYDSL SNRLSILDRL LHTHPIWLQL
110 120 130 140 150
SLSEEEAAEV LQAQPPGIFL VRKSSKMQKK VLSLRLPCEF GAPLKEFTIK
160 170 180 190 200
ESTYTFSLEG SGISFADLFR LIAFYCISRD VLPFTLKLPY AISTAKTESQ
210 220 230 240 250
LEELAQLGLN FWSSSADNKP LNSPPPHRPL PSAGICPASL RQLCLINGVH
260 270 280 290 300
SIKTRTPSEL ECSQTNGALC FINPLFLKVH SQDLSTGPKR PSTRTPNANG
310 320 330 340 350
TERPRSPPPR PPPPAINSLH TSPGLSRTEP QTSMPETVNH SKHGNVALLG
360 370 380 390 400
TKPTPIPPPR LKKQASFLEA ESSAKTLTAR RPSRRSEPEP ELELELEMGT
410 420 430 440 450
AGHAGGAPPR DAPGDCTRAP PPGSESQPPP CHGARQRLSD MSLSTSSSDS
460 470 480 490 500
LEFDRSMPLY GYEADTTSSL EDYEGESDQE TMAPPIKSKK KRNSSFVLPK
510 520 530 540 550
LVKSQLRKMS GVFSSFMTPE KRMVRRIAEL SRDKCTYFGC LVQDYVSFLK
560 570 580 590 600
ENKECHVSST DMLQTIRQFM TQVKNYLSQS SELDPPIESL IPEDQIDVVL
610 620 630 640 650
EKAMHKCILK PLKGHVEAML KDFHTADGSW KQLKENLQLV RQRNPQELGV
660 670 680 690 700
FAPTPDLMEL EKIKLKFMTM QKMYSPEKKV MLLLRVCKLI YTVMENNSGR
710 720 730 740 750
MYGADDFLPV LTYVIAQCDM LELDTEIEYM MELLDPSLLH GEGGYYLTSA
760 770 780 790 800
YGALSLIKNF QEEQAARLLS SEARDTLRQW HKRRTTNRTI PSVDDFQNYL
810 820 830 840 850
RVAFQEVNSG CTGKTLLVRP YITTEDVCQL CAEKFKVEDP EEYSLFLFVD
860 870 880 890 900
ETWQQLAEDT YPQKIKAELH SRPQPHIFHF VYKRIKSDPY GVIFQNGEDL

TPS
Note: Derived from EST data.
Length:903
Mass (Da):101,559
Last modified:July 27, 2011 - v3
Checksum:iA79017A8B4E7949B
GO
Isoform 2 (identifier: Q9D684-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:850
Mass (Da):95,677
Checksum:i1830BA07DDCEE5AD
GO

Sequence cautioni

The sequence AAH05529 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH40390 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAB29425 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC30697 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE29760 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAE30311 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAE31576 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180D → G in BAE29760 (PubMed:16141072).Curated1
Sequence conflicti204L → I in BAE29760 (PubMed:16141072).Curated1
Sequence conflicti204L → I in BAE31576 (PubMed:16141072).Curated1
Sequence conflicti221L → P in AAH40390 (PubMed:15489334).Curated1
Sequence conflicti385R → S in AAH40390 (PubMed:15489334).Curated1
Sequence conflicti448S → Y in BAB29425 (PubMed:16141072).Curated1
Sequence conflicti491K → R in BAB29425 (PubMed:16141072).Curated1
Sequence conflicti694M → V in BAE30311 (PubMed:16141072).Curated1
Sequence conflicti729Y → C in BAE29760 (PubMed:16141072).Curated1
Sequence conflicti748T → A in BAE30311 (PubMed:16141072).Curated1
Sequence conflicti801R → Q in BAC30697 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0075811 – 53Missing in isoform 2. 2 PublicationsAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014548 mRNA Translation: BAB29425.1 Different initiation.
AK040763 mRNA Translation: BAC30697.1 Different initiation.
AK150680 mRNA Translation: BAE29760.1 Sequence problems.
AK151332 mRNA Translation: BAE30311.1 Sequence problems.
AK152893 mRNA Translation: BAE31576.1 Sequence problems.
AL672100 Genomic DNA No translation available.
BC005529 mRNA Translation: AAH05529.1 Different initiation.
BC040390 mRNA Translation: AAH40390.3 Sequence problems.
BI660407 mRNA No translation available.
RefSeqiNP_083000.5, NM_028724.4
XP_006500332.1, XM_006500269.1 [Q9D684-1]
XP_006500333.1, XM_006500270.3 [Q9D684-1]
XP_006500334.1, XM_006500271.3 [Q9D684-1]
UniGeneiMm.228799

Genome annotation databases

EnsembliENSMUST00000110005; ENSMUSP00000105632; ENSMUSG00000001768 [Q9D684-1]
GeneIDi74030
KEGGimmu:74030

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRIN2_MOUSE
AccessioniPrimary (citable) accession number: Q9D684
Secondary accession number(s): A8Y5L0
, Q3U6Z8, Q3UAK3, Q3UC54, Q99K06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health