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Protein

SPRY domain-containing SOCS box protein 1

Gene

Spsb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664. Neddylation.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 1
Short name:
SSB-1
Gene namesi
Name:Spsb1
Synonyms:Ssb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1921896. Spsb1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384731 – 273SPRY domain-containing SOCS box protein 1Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D5L7.
PRIDEiQ9D5L7.

PTM databases

iPTMnetiQ9D5L7.
PhosphoSitePlusiQ9D5L7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039911.
ExpressionAtlasiQ9D5L7. baseline and differential.
GenevisibleiQ9D5L7. MM.

Interactioni

Subunit structurei

Component of the probable ECS(SPSB1) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB1 (By similarity). Interacts with CUL5, RNF7, ELOB and ELOC (By similarity). Directly interacts with MET tyrosine kinase domain in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction (PubMed:16369487). When phosphorylated, interacts with RASA1 without affecting its stability (By similarity). Interacts (via B30.2/SPRY domain) with PAWR; this interaction is direct and occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:16498413, PubMed:20561531).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PAWRQ96IZ02EBI-8821912,EBI-595869From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-29000N.
IntActiQ9D5L7. 1 interactor.
STRINGi10090.ENSMUSP00000048969.

Structurei

3D structure databases

ProteinModelPortaliQ9D5L7.
SMRiQ9D5L7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 231B30.2/SPRYPROSITE-ProRule annotationAdd BLAST199
Domaini232 – 273SOCS boxPROSITE-ProRule annotationAdd BLAST42

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The B30.2/SPRY domain is involved in MET and PAWR binding (PubMed:16369487, PubMed:20561531).By similarity2 Publications

Sequence similaritiesi

Belongs to the SPSB family.Curated

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG055793.
InParanoidiQ9D5L7.
KOiK10343.
OMAiMGQKVPG.
OrthoDBiEOG091G0DF0.
PhylomeDBiQ9D5L7.
TreeFamiTF312822.

Family and domain databases

InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom_sf.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
PfamiView protein in Pfam
PF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
SMARTiView protein in SMART
SM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D5L7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQKVTGGIK TVDMRDPTYR PLKQELQGLD YCKPTRLDLL LDMPPVSYDV
60 70 80 90 100
QLLHSWNNND RSLNVFVKED DKLIFHRHPV AQSTDAIRGK VGYTRGLHVW
110 120 130 140 150
QITWAMRQRG THAVVGVATA DAPLHSVGYT TLVGNNHESW GWDLGRNRLY
160 170 180 190 200
HDGKNQPSKT YPAFLEPDET FIVPDSFLVA LDMDDGTLSF IVDGQYMGVA
210 220 230 240 250
FRGLKGKKLY PVVSAVWGHC EIRMRYLNGL DPEPLPLMDL CRRSVRLALG
260 270
KERLGAIPAL PLPASLKAYL LYQ
Length:273
Mass (Da):30,785
Last modified:June 1, 2001 - v1
Checksum:i2CBA3B50A3240177
GO
Isoform 2 (identifier: Q9D5L7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Show »
Length:168
Mass (Da):18,656
Checksum:i4E94AAA0ED006A4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11T → S in BAC40957 (Ref. 1) Curated1
Sequence conflicti125H → R in BAC40957 (Ref. 1) Curated1
Sequence conflicti146R → H in BAC40957 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186121 – 105Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403036 mRNA. Translation: AAL57355.1.
AK015181 mRNA. Translation: BAB29738.1.
AK003707 mRNA. Translation: BAC25049.1.
AK030417 mRNA. Translation: BAC26954.1.
AK040909 mRNA. Translation: BAC30739.1.
AK089767 mRNA. Translation: BAC40957.1.
AK160081 mRNA. Translation: BAE35613.1.
AK172334 mRNA. Translation: BAE42952.1.
BC046787 mRNA. Translation: AAH46787.1.
BC057563 mRNA. Translation: AAH57563.1.
BC089357 mRNA. Translation: AAH89357.1.
CCDSiCCDS18966.1. [Q9D5L7-1]
RefSeqiNP_083311.1. NM_029035.2. [Q9D5L7-1]
XP_006539297.1. XM_006539234.2. [Q9D5L7-1]
XP_011248646.1. XM_011250344.2. [Q9D5L7-1]
XP_011248647.1. XM_011250345.2. [Q9D5L7-1]
UniGeneiMm.30.

Genome annotation databases

EnsembliENSMUST00000038562; ENSMUSP00000048969; ENSMUSG00000039911. [Q9D5L7-1]
ENSMUST00000105684; ENSMUSP00000101309; ENSMUSG00000039911. [Q9D5L7-1]
ENSMUST00000105685; ENSMUSP00000101310; ENSMUSG00000039911. [Q9D5L7-1]
GeneIDi74646.
KEGGimmu:74646.
UCSCiuc008vxf.1. mouse. [Q9D5L7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPSB1_MOUSE
AccessioniPrimary (citable) accession number: Q9D5L7
Secondary accession number(s): Q5FWI0, Q8BJA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: December 20, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families