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Q9D5J6 (SHPK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sedoheptulokinase
Short name=SHK
EC=2.7.1.14
Alternative name(s):
Carbohydrate kinase-like protein
Gene names
Name:Shpk
Synonyms:Carkl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a modulator of macrophage activation through control of glucose metabolism. Ref.4 Ref.5

Catalytic activity

ATP + sedoheptulose = ADP + sedoheptulose 7-phosphate. Ref.5

Subcellular location

Cytoplasm Ref.5.

Induction

Up-regulated by IL-4 and IL-13. Down-regulated by LPS. Ref.5

Sequence similarities

Belongs to the FGGY kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.19 mM for sedoheptulose Ref.4

Vmax=0.128 nmol/min/mg enzyme (at 30 degrees Celsius)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Sedoheptulokinase
PRO_0000059565

Experimental info

Mutagenesis141T → M: Decreases sedoheptulose kinase activity; when associated with K-15. Ref.5
Mutagenesis151T → K: Decreases sedoheptulose kinase activity; when associated with M-14. Ref.5
Mutagenesis1251W → D: Decreases sedoheptulose kinase activity. Ref.5
Mutagenesis1261Q → K: Decreases sedoheptulose kinase activity. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9D5J6 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: B3638772519DBA3C

FASTA47651,303
        10         20         30         40         50         60 
MASRPVTLGI DLGTTSVKAA LLEAAPSLPS GFVVLASCAR AARAETESAV AGPQGREQDV 

        70         80         90        100        110        120 
TRIIQALNEC LDALPRQQLQ RVRGIGVSGQ MHGILFWKAG QGCEWMEGGP AFVFEPRAVS 

       130        140        150        160        170        180 
HLVTWQDGRC NSSFLASLPK PDSHLSVATG FGCATIFWLL KNSPEFLKSY DAAGTIQDYV 

       190        200        210        220        230        240 
VAMLCGLPRP LMSDQNAASW GYFNTQSQSW NLDTLEKAGF PIHLLPDIAE PGSMAGRTSH 

       250        260        270        280        290        300 
TWFEIPKGTQ VGIALGDLQA SVYSCMGQRT DAVLNISTSV QLAASMPVGF QPLQTPDPAA 

       310        320        330        340        350        360 
PVAFFPYFDR TYLGVAASLN GGNVLATFVH MLVQWMADLG LEVEESTVYS RMIQAAAQQK 

       370        380        390        400        410        420 
DTHLTITPTV LGERHLPDQL ASVTRISSSD LSLGHVTRAL CRGIVQNLHS MLPFQQLKEW 

       430        440        450        460        470 
GVARVVGSGS ALSRNEVLKQ EVQRAFPFPV CFGQDVDAAF GAALVMLQRD LSQKEP

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency."
Kardon T., Stroobant V., Veiga-da-Cunha M., Schaftingen E.V.
FEBS Lett. 582:3330-3334(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[5]"The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism."
Haschemi A., Kosma P., Gille L., Evans C.R., Burant C.F., Starkl P., Knapp B., Haas R., Schmid J.A., Jandl C., Amir S., Lubec G., Park J., Esterbauer H., Bilban M., Brizuela L., Pospisilik J.A., Otterbein L.E., Wagner O.
Cell Metab. 15:813-826(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-14; THR-15; TRP-125 AND GLN-126, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK015273 mRNA. Translation: BAB29776.1.
AL663116, AL670399 Genomic DNA. Translation: CAI24581.1.
AL670399, AL663116 Genomic DNA. Translation: CAI24796.1.
BC054721 mRNA. Translation: AAH54721.1.
BC056433 mRNA. Translation: AAH56433.1.
IPIIPI00108042.
RefSeqNP_083307.1. NM_029031.3.
UniGeneMm.200905.

3D structure databases

ProteinModelPortalQ9D5J6.
SMRQ9D5J6. Positions 8-236.
ModBaseSearch...

PTM databases

PhosphoSiteQ9D5J6.

Proteomic databases

PaxDbQ9D5J6.
PRIDEQ9D5J6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006105; ENSMUSP00000006105; ENSMUSG00000005951.
GeneID74637.
KEGGmmu:74637.

Organism-specific databases

CTD23729.
MGIMGI:1921887. Shpk.

Phylogenomic databases

eggNOGCOG0554.
GeneTreeENSGT00390000010693.
HOGENOMHOG000008078.
HOVERGENHBG050796.
InParanoidQ9D5J6.
KOK11214.
OMAFQCSVYS.

Gene expression databases

ArrayExpressQ9D5J6.
BgeeQ9D5J6.
CleanExMM_SHPK.
GenevestigatorQ9D5J6.
GermOnlineENSMUSG00000005951. Mus musculus.

Family and domain databases

InterProIPR018484. Carb_kinase_FGGY_N.
[Graphical view]
PfamPF00370. FGGY_N. 1 hit.
[Graphical view]
PROSITEPS00933. FGGY_KINASES_1. False negative.
PS00445. FGGY_KINASES_2. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio341265.
SOURCESearch...

Entry information

Entry nameSHPK_MOUSE
AccessionPrimary (citable) accession number: Q9D5J6
Secondary accession number(s): Q5SSE0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents