Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Splicing factor 3A subunit 3

Gene

Sf3a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri406 – 43732Matrin-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 3
Alternative name(s):
SF3a60
Spliceosome-associated protein 61
Short name:
SAP 61
Gene namesi
Name:Sf3a3
Synonyms:Sap61
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922312. Sf3a3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Splicing factor 3A subunit 3PRO_0000174319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei54 – 541PhosphoserineBy similarity
Modified residuei121 – 1211PhosphoserineBy similarity
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei299 – 2991PhosphoserineBy similarity
Modified residuei365 – 3651PhosphoserineBy similarity
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei475 – 4751PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D554.
MaxQBiQ9D554.
PaxDbiQ9D554.
PRIDEiQ9D554.

PTM databases

iPTMnetiQ9D554.
PhosphoSiteiQ9D554.
SwissPalmiQ9D554.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028902.
CleanExiMM_SF3A3.
ExpressionAtlasiQ9D554. baseline and differential.
GenevisibleiQ9D554. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A1, through its N-terminal region (By similarity).By similarity

Protein-protein interaction databases

BioGridi217189. 3 interactions.
IntActiQ9D554. 6 interactions.
MINTiMINT-1853954.
STRINGi10090.ENSMUSP00000030734.

Structurei

3D structure databases

ProteinModelPortaliQ9D554.
SMRiQ9D554. Positions 71-107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi354 – 3629Poly-Glu

Sequence similaritiesi

Belongs to the SF3A3 family.Curated
Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri406 – 43732Matrin-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2636. Eukaryota.
COG5188. LUCA.
GeneTreeiENSGT00530000063402.
HOGENOMiHOG000160935.
HOVERGENiHBG054452.
InParanoidiQ9D554.
KOiK12827.
OMAiPKKDTER.
OrthoDBiEOG091G059G.
PhylomeDBiQ9D554.
TreeFamiTF315227.

Family and domain databases

InterProiIPR024598. DUF3449.
IPR031776. SF3A3.
IPR031774. SF3A3_dom.
IPR021966. SF3a60_bindingd.
IPR000690. Znf_C2H2_matrin.
[Graphical view]
PANTHERiPTHR12786:SF2. PTHR12786:SF2. 1 hit.
PfamiPF11931. DUF3449. 1 hit.
PF16837. SF3A3. 1 hit.
PF12108. SF3a60_bindingd. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY
60 70 80 90 100
MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLKQ IKEFHRKHPN
110 120 130 140 150
EICVPMSVEF EELLKARENP SEEAQNLVEF TDEEGYGRYL DLHDCYLKYI
160 170 180 190 200
NLKASEKLDY ITYLSIFDQL FDIPKERKNA EYKRYLEMLL EYLQDYTDRV
210 220 230 240 250
KPLQDQNELF GKIQTDFEKK WDNGTFPGWP KETSSALTHA GAHLDLSAFS
260 270 280 290 300
SWEELASLGL DRLKSALLAL GLKCGGTLEE RAQRLFSTKG KSLESLDTSL
310 320 330 340 350
FAKNPKSKGT KRDTERNKDI AFLEAQIYEY VEILGEQRQL THENVQRKQA
360 370 380 390 400
RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK PIPYWLYKLH
410 420 430 440 450
GLNINYNCEI CGNYTYRGPK AFQRHFAEWR HAHGMRCLGI PNTAHFANVT
460 470 480 490 500
QIEDAVSLWA KLKLQKASER WQPDTEEEYE DSSGNVVNKK TYEDLKRQGL

L
Length:501
Mass (Da):58,842
Last modified:August 29, 2001 - v2
Checksum:i46EE0928FC8271D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC009141 mRNA. Translation: AAH09141.1.
AK015776 mRNA. Translation: BAB29971.1.
CCDSiCCDS38875.1.
RefSeqiNP_083433.1. NM_029157.3.
UniGeneiMm.25779.

Genome annotation databases

EnsembliENSMUST00000030734; ENSMUSP00000030734; ENSMUSG00000028902.
GeneIDi75062.
KEGGimmu:75062.
UCSCiuc008uqx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC009141 mRNA. Translation: AAH09141.1.
AK015776 mRNA. Translation: BAB29971.1.
CCDSiCCDS38875.1.
RefSeqiNP_083433.1. NM_029157.3.
UniGeneiMm.25779.

3D structure databases

ProteinModelPortaliQ9D554.
SMRiQ9D554. Positions 71-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217189. 3 interactions.
IntActiQ9D554. 6 interactions.
MINTiMINT-1853954.
STRINGi10090.ENSMUSP00000030734.

PTM databases

iPTMnetiQ9D554.
PhosphoSiteiQ9D554.
SwissPalmiQ9D554.

Proteomic databases

EPDiQ9D554.
MaxQBiQ9D554.
PaxDbiQ9D554.
PRIDEiQ9D554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030734; ENSMUSP00000030734; ENSMUSG00000028902.
GeneIDi75062.
KEGGimmu:75062.
UCSCiuc008uqx.1. mouse.

Organism-specific databases

CTDi10946.
MGIiMGI:1922312. Sf3a3.

Phylogenomic databases

eggNOGiKOG2636. Eukaryota.
COG5188. LUCA.
GeneTreeiENSGT00530000063402.
HOGENOMiHOG000160935.
HOVERGENiHBG054452.
InParanoidiQ9D554.
KOiK12827.
OMAiPKKDTER.
OrthoDBiEOG091G059G.
PhylomeDBiQ9D554.
TreeFamiTF315227.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSf3a3. mouse.
PROiQ9D554.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028902.
CleanExiMM_SF3A3.
ExpressionAtlasiQ9D554. baseline and differential.
GenevisibleiQ9D554. MM.

Family and domain databases

InterProiIPR024598. DUF3449.
IPR031776. SF3A3.
IPR031774. SF3A3_dom.
IPR021966. SF3a60_bindingd.
IPR000690. Znf_C2H2_matrin.
[Graphical view]
PANTHERiPTHR12786:SF2. PTHR12786:SF2. 1 hit.
PfamiPF11931. DUF3449. 1 hit.
PF16837. SF3A3. 1 hit.
PF12108. SF3a60_bindingd. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSF3A3_MOUSE
AccessioniPrimary (citable) accession number: Q9D554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.