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Protein

Ethanolamine kinase 1

Gene

Etnk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylathanolamine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2), Ethanolamine kinase 1 (Etnk1), Choline kinase alpha (Chka)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Ept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00741.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine kinase 1 (EC:2.7.1.82)
Short name:
EKI 1
Gene namesi
Name:Etnk1
Synonyms:Eki1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1922570. Etnk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Ethanolamine kinase 1PRO_0000206228Add
BLAST

Proteomic databases

EPDiQ9D4V0.
MaxQBiQ9D4V0.
PaxDbiQ9D4V0.
PRIDEiQ9D4V0.

PTM databases

iPTMnetiQ9D4V0.
PhosphoSiteiQ9D4V0.

Expressioni

Gene expression databases

CleanExiMM_ETNK1.
ExpressionAtlasiQ9D4V0. baseline and differential.
GenevisibleiQ9D4V0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032413.

Structurei

3D structure databases

ProteinModelPortaliQ9D4V0.
SMRiQ9D4V0. Positions 57-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiKOG4720. Eukaryota.
COG0510. LUCA.
GeneTreeiENSGT00530000062991.
HOVERGENiHBG018981.
InParanoidiQ9D4V0.
KOiK00894.
OMAiQEEMAWM.
OrthoDBiEOG72NRQH.
TreeFamiTF313549.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D4V0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANYIHVPPG SPEVPKLDVT VQDQEEQRCR DGALSLLRHL RPHWDPREVT
60 70 80 90 100
LQLFTDGITN KLIACYVGDT MEDVVLVRIY GNKTELLVDR DEEVKSFRVL
110 120 130 140 150
QAHGCAPQLY CTFNNGLCYE FIQGEALDPQ HVCNPAIFRL IARQLAKIHA
160 170 180 190 200
IHAHNGWIPK SNLWLKMGKY FSLIPTGFAD ENINKRFLSE IPSPQLLQEE
210 220 230 240 250
MTWMKELLSS LGSPVVLCHN DLLCKNIIYN EKQGDVQFID YEYSGYNYLA
260 270 280 290 300
YDIGNHFNEF AGVSDVDYSL YPDRELQGQW LRSYLEAYKE YKGFGSDVTE
310 320 330 340 350
KEVETLFIQV NQFALASHFF WGLWALIQAK YSTIEFDFLG YAVVRFNQYF
360
KMKPEVTALK MPE
Length:363
Mass (Da):41,984
Last modified:September 18, 2013 - v2
Checksum:iEB74674AA31F6C70
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261A → L in AK016135 (PubMed:16141072).Curated
Sequence conflicti280 – 2812WL → CV in AK016135 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016135 mRNA. No translation available.
AK044502 mRNA. Translation: BAC31953.1.
AC127354 Genomic DNA. No translation available.
AC132955 Genomic DNA. No translation available.
CH466572 Genomic DNA. Translation: EDL10659.1.
CCDSiCCDS57468.1.
RefSeqiNP_083526.2. NM_029250.2.
UniGeneiMm.272548.

Genome annotation databases

EnsembliENSMUST00000032413; ENSMUSP00000032413; ENSMUSG00000030275.
GeneIDi75320.
KEGGimmu:75320.
UCSCiuc009eqe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016135 mRNA. No translation available.
AK044502 mRNA. Translation: BAC31953.1.
AC127354 Genomic DNA. No translation available.
AC132955 Genomic DNA. No translation available.
CH466572 Genomic DNA. Translation: EDL10659.1.
CCDSiCCDS57468.1.
RefSeqiNP_083526.2. NM_029250.2.
UniGeneiMm.272548.

3D structure databases

ProteinModelPortaliQ9D4V0.
SMRiQ9D4V0. Positions 57-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032413.

PTM databases

iPTMnetiQ9D4V0.
PhosphoSiteiQ9D4V0.

Proteomic databases

EPDiQ9D4V0.
MaxQBiQ9D4V0.
PaxDbiQ9D4V0.
PRIDEiQ9D4V0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032413; ENSMUSP00000032413; ENSMUSG00000030275.
GeneIDi75320.
KEGGimmu:75320.
UCSCiuc009eqe.1. mouse.

Organism-specific databases

CTDi55500.
MGIiMGI:1922570. Etnk1.

Phylogenomic databases

eggNOGiKOG4720. Eukaryota.
COG0510. LUCA.
GeneTreeiENSGT00530000062991.
HOVERGENiHBG018981.
InParanoidiQ9D4V0.
KOiK00894.
OMAiQEEMAWM.
OrthoDBiEOG72NRQH.
TreeFamiTF313549.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
ReactomeiR-MMU-1483213. Synthesis of PE.

Miscellaneous databases

ChiTaRSiEtnk1. mouse.
PROiQ9D4V0.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ETNK1.
ExpressionAtlasiQ9D4V0. baseline and differential.
GenevisibleiQ9D4V0. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Retina and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney and Spleen.

Entry informationi

Entry nameiEKI1_MOUSE
AccessioniPrimary (citable) accession number: Q9D4V0
Secondary accession number(s): F6Y9V2, Q8BXQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: September 18, 2013
Last modified: June 8, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.