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Protein

Angiomotin-like protein 1

Gene

Amotl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.By similarity

GO - Molecular functioni

GO - Biological processi

  • establishment of cell polarity involved in ameboidal cell migration Source: MGI
  • hippo signaling Source: MGI
  • positive regulation of blood vessel endothelial cell migration Source: MGI
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiomotin-like protein 1
Alternative name(s):
junction-enriched and -associated protein
Short name:
JEAP
Gene namesi
Name:Amotl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1922973. Amotl1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • bicellular tight junction Source: MGI
  • cell junction Source: MGI
  • COP9 signalosome Source: Ensembl
  • cytoplasm Source: MGI
  • cytoplasmic vesicle Source: MGI
  • lamellipodium Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001906711 – 968Angiomotin-like protein 1Add BLAST968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253PhosphoserineBy similarity1
Modified residuei281PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei731PhosphoserineBy similarity1
Modified residuei804PhosphoserineBy similarity1
Modified residuei816PhosphoserineCombined sources1
Modified residuei840PhosphoserineBy similarity1
Modified residuei912PhosphoserineBy similarity1
Modified residuei918PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated by NEDD4, leading to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9D4H4.
PeptideAtlasiQ9D4H4.
PRIDEiQ9D4H4.

PTM databases

iPTMnetiQ9D4H4.
PhosphoSitePlusiQ9D4H4.

Expressioni

Tissue specificityi

Expressed in exocrine glands, including pancreas, submandibular gland, lacrimal gland, parotid gland and sublingual gland (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000013076.
CleanExiMM_AMOTL1.
ExpressionAtlasiQ9D4H4. baseline and differential.
GenevisibleiQ9D4H4. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217693. 6 interactors.
STRINGi10090.ENSMUSP00000013220.

Structurei

3D structure databases

ProteinModelPortaliQ9D4H4.
SMRiQ9D4H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili271 – 291Sequence analysisAdd BLAST21
Coiled coili449 – 645Sequence analysisAdd BLAST197
Coiled coili676 – 705Sequence analysisAdd BLAST30
Coiled coili748 – 773Sequence analysisAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi965 – 968PDZ-binding4

Sequence similaritiesi

Belongs to the angiomotin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJU5. Eukaryota.
ENOG410XSY2. LUCA.
GeneTreeiENSGT00530000063846.
HOGENOMiHOG000233789.
HOVERGENiHBG066485.
InParanoidiQ9D4H4.
KOiK06104.
OMAiSKTQEHG.
OrthoDBiEOG091G02BL.
PhylomeDBiQ9D4H4.
TreeFamiTF333368.

Family and domain databases

InterProiIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PfamiPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSiPR01807. ANGIOMOTIN.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D4H4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRAKSRRGP CEPVLRAPPP ICYSPSSPVQ ILEDPAYFYP DLQLYSGRHE
60 70 80 90 100
ASTLTVEASG GLRGKSVEDP LSSFHSPNFL RTPEVEMRGS EDVASGRVLQ
110 120 130 140 150
RLIQEQLRYG TPTENMNLLA IQHQATGSAG PAHATTNFSS TETLTQEDPQ
160 170 180 190 200
MVYQSARQEP QGQEHQGDNT VMEKQVRSTQ PQQNNEELPT YEEAKAQSQF
210 220 230 240 250
FRGQQQQQQQ QQQQQQQQQQ QGQGPLSHTY YMAGGTSQKS RTEGRPTVNR
260 270 280 290 300
ANSGQAHKDE ALKELKQGHV RSLSERIMQL SLERNGAKQH LPSSGNGKSF
310 320 330 340 350
KAGGEPSPAQ PVCKALDPRG PPPEYPFKTK PMKSPVSKNQ DHGLYYNDQH
360 370 380 390 400
PGVLHEMVKP YPAPQPARTE VAVLRYQPPP EYGVTSRPCQ LPFPSTVQQH
410 420 430 440 450
SPMSSQTSSI GGTLHSVSLP LPLPISLAAS QPLPASPNQQ LGPDAFAIVE
460 470 480 490 500
RAQQMVEILT EENRVLHQEL QGCYDNADKL HKFEKELQSI SEAYESLVKS
510 520 530 540 550
TTKRESLDKA MRTKLEGEIR RLHDFNRDLR DRLETANRQL SSREYDGHED
560 570 580 590 600
KAAESHYVSQ NKEFLKEKEK LEMELAAVRT ASEDHRRHIE ILDQALSNAQ
610 620 630 640 650
ARVIKLEEEL REKQAYVEKV EKLQQALTQL QSACEKRGQM ERRLRTWLER
660 670 680 690 700
ELDALRTQQK HGTGPPVSLP ECNAPALMEL VREKEERILA LEADMTKWEQ
710 720 730 740 750
KYLEESTIRH FAMSAAAAAT AERDTTISNH SRNGSYGESS LEAHIWPEEE
760 770 780 790 800
EVVQANRRCQ DMEYTIKNLH AKIIEKDAMI KVLQQRSRKD AGKTDSASLR
810 820 830 840 850
PARSVPSIAA ATGTHSRQTS LTSSQLTEEK KEEKTTWKGS IGFLLGKEHQ
860 870 880 890 900
GQASAPLLPT TPASALSLPA STTSASSTHA KTGSKDSSTQ TDKSTELFWP
910 920 930 940 950
SMASLPSRGR LSTAPSNSPI LKHPAAKGAV EKQENSPGHG KASEHRGRVS
960
NLLHKPEFPD GEMMEVLI
Length:968
Mass (Da):107,950
Last modified:September 5, 2012 - v2
Checksum:i400DCC70DD21E789
GO
Isoform 2 (identifier: Q9D4H4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.

Note: No experimental confirmation available.
Show »
Length:882
Mass (Da):98,424
Checksum:i88AAFE83FFA56046
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221Missing in BAD90163 (PubMed:19468303).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0440801 – 86Missing in isoform 2. 1 PublicationAdd BLAST86

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016526 mRNA. Translation: BAB30287.1.
CT030247 Genomic DNA. No translation available.
CT485607 Genomic DNA. No translation available.
BC151000 mRNA. Translation: AAI51001.1.
BC151004 mRNA. Translation: AAI51005.1.
BC151007 mRNA. Translation: AAI51008.1.
AK220238 mRNA. Translation: BAD90163.1.
CCDSiCCDS40535.1. [Q9D4H4-1]
RefSeqiNP_001074864.1. NM_001081395.1. [Q9D4H4-1]
XP_006510731.1. XM_006510668.1. [Q9D4H4-2]
XP_006510732.1. XM_006510669.3. [Q9D4H4-2]
XP_006510733.1. XM_006510670.1. [Q9D4H4-2]
UniGeneiMm.159552.

Genome annotation databases

EnsembliENSMUST00000013220; ENSMUSP00000013220; ENSMUSG00000013076. [Q9D4H4-1]
ENSMUST00000160770; ENSMUSP00000124281; ENSMUSG00000013076. [Q9D4H4-2]
GeneIDi75723.
KEGGimmu:75723.
UCSCiuc009oep.1. mouse. [Q9D4H4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016526 mRNA. Translation: BAB30287.1.
CT030247 Genomic DNA. No translation available.
CT485607 Genomic DNA. No translation available.
BC151000 mRNA. Translation: AAI51001.1.
BC151004 mRNA. Translation: AAI51005.1.
BC151007 mRNA. Translation: AAI51008.1.
AK220238 mRNA. Translation: BAD90163.1.
CCDSiCCDS40535.1. [Q9D4H4-1]
RefSeqiNP_001074864.1. NM_001081395.1. [Q9D4H4-1]
XP_006510731.1. XM_006510668.1. [Q9D4H4-2]
XP_006510732.1. XM_006510669.3. [Q9D4H4-2]
XP_006510733.1. XM_006510670.1. [Q9D4H4-2]
UniGeneiMm.159552.

3D structure databases

ProteinModelPortaliQ9D4H4.
SMRiQ9D4H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217693. 6 interactors.
STRINGi10090.ENSMUSP00000013220.

PTM databases

iPTMnetiQ9D4H4.
PhosphoSitePlusiQ9D4H4.

Proteomic databases

PaxDbiQ9D4H4.
PeptideAtlasiQ9D4H4.
PRIDEiQ9D4H4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000013220; ENSMUSP00000013220; ENSMUSG00000013076. [Q9D4H4-1]
ENSMUST00000160770; ENSMUSP00000124281; ENSMUSG00000013076. [Q9D4H4-2]
GeneIDi75723.
KEGGimmu:75723.
UCSCiuc009oep.1. mouse. [Q9D4H4-1]

Organism-specific databases

CTDi154810.
MGIiMGI:1922973. Amotl1.

Phylogenomic databases

eggNOGiENOG410IJU5. Eukaryota.
ENOG410XSY2. LUCA.
GeneTreeiENSGT00530000063846.
HOGENOMiHOG000233789.
HOVERGENiHBG066485.
InParanoidiQ9D4H4.
KOiK06104.
OMAiSKTQEHG.
OrthoDBiEOG091G02BL.
PhylomeDBiQ9D4H4.
TreeFamiTF333368.

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.

Miscellaneous databases

ChiTaRSiAmotl1. mouse.
PROiQ9D4H4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000013076.
CleanExiMM_AMOTL1.
ExpressionAtlasiQ9D4H4. baseline and differential.
GenevisibleiQ9D4H4. MM.

Family and domain databases

InterProiIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PfamiPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSiPR01807. ANGIOMOTIN.
ProtoNetiSearch...

Entry informationi

Entry nameiAMOL1_MOUSE
AccessioniPrimary (citable) accession number: Q9D4H4
Secondary accession number(s): B9EKN5, Q571F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 5, 2012
Last modified: November 2, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.