Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9D4C5 (EAF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ELL-associated factor 1
Gene names
Name:Eaf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional transactivator of ELL and ELL2 elongation activities By similarity.

Subunit structure

Interacts with ELL and ELL2 By similarity.

Subcellular location

Nucleus › Cajal body By similarity. Nucleus speckle By similarity.

Sequence similarities

Belongs to the EAF family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentCajal body

Inferred from electronic annotation. Source: UniProtKB-SubCell

ELL-EAF complex

Inferred from electronic annotation. Source: InterPro

nuclear speck

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268ELL-associated factor 1
PRO_0000130335

Regions

Region182 – 26281Necessary for transactivation activity By similarity
Compositional bias127 – 16842Pro-rich
Compositional bias188 – 21124Ser-rich

Amino acid modifications

Modified residue1581Phosphoserine Ref.3
Modified residue1651Phosphoserine Ref.3 Ref.4 Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9D4C5 [UniParc].

Last modified March 1, 2003. Version 2.
Checksum: 02ACBA8F33200C20

FASTA26828,967
        10         20         30         40         50         60 
MNGTANPLLD REEHCLRLGE SFEKRPRASF HTIRYDFKPA SIDTSCEGEL QVGKGDEVTI 

        70         80         90        100        110        120 
TLPHIPGSTP PMTVFKGNKR PYQKDCVLII NHDTGEYVLE KLSSSIQVKK TRAEGSSKIQ 

       130        140        150        160        170        180 
ARMEQQPARP PQPSQPPPPP PPMPFRAPTK PPAGPKTSPL KDNPSPEPQL DDIKRELRAE 

       190        200        210        220        230        240 
VDIIEQMSSS SGSSSSDSES SSGSDDDSSS SAGEDNGPAS PPQPSHQQPY NSRPAVANGT 

       250        260 
SRPQGSSQLM NTLRNDLQLS ESGSDSDD

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain cortex, Retina and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Embryonic brain.
[3]"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line."
Shu H., Chen S., Bi Q., Mumby M., Brekken D.L.
Mol. Cell. Proteomics 3:279-286(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158 AND SER-165, MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, MASS SPECTROMETRY.
Tissue: Liver.
[5]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, MASS SPECTROMETRY.
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK016628 mRNA. Translation: BAB30346.2.
AK044181 mRNA. Translation: BAC31806.1.
AK044737 mRNA. Translation: BAC32058.1.
BC079658 mRNA. Translation: AAH79658.1.
IPIIPI00187572.
RefSeqNP_083208.1. NM_028932.4.
UniGeneMm.37770.

3D structure databases

ProteinModelPortalQ9D4C5.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000022446.

PTM databases

PhosphoSiteQ9D4C5.

Proteomic databases

PRIDEQ9D4C5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022446; ENSMUSP00000022446; ENSMUSG00000021890.
GeneID74427.
KEGGmmu:74427.
UCSCuc007sxt.2. mouse.

Organism-specific databases

CTD85403.
MGIMGI:1921677. Eaf1.

Phylogenomic databases

eggNOGNOG273677.
GeneTreeENSGT00390000017724.
HOGENOMHOG000070137.
HOVERGENHBG054898.
InParanoidQ9D4C5.
KOK15186.
OMASHQQPYS.
OrthoDBEOG4868DG.

Gene expression databases

BgeeQ9D4C5.
CleanExMM_EAF1.
GenevestigatorQ9D4C5.
GermOnlineENSMUSG00000021890. Mus musculus.

Family and domain databases

InterProIPR027093. EAF_fam.
IPR019194. Tscrpt_elong_fac_Eaf_N.
[Graphical view]
PANTHERPTHR15970. PTHR15970. 1 hit.
PfamPF09816. EAF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio340729.
SOURCESearch...

Entry information

Entry nameEAF1_MOUSE
AccessionPrimary (citable) accession number: Q9D4C5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents