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Protein

Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2

Gene

Sohlh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor, which may be involved in spermatogenesis and oogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

  • oocyte differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • primary ovarian follicle growth Source: MGI
  • regulation of gene expression Source: MGI
  • spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Oogenesis, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
Gene namesi
Name:Sohlh2
Synonyms:Tohlh2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1921684. Sohlh2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2PRO_0000315701Add
BLAST

Proteomic databases

MaxQBiQ9D489.
PaxDbiQ9D489.
PRIDEiQ9D489.

Expressioni

Tissue specificityi

Preferentially expressed in the adult ovary and testis.2 Publications

Developmental stagei

In the embryonic gonads it is expressed as early as E13.5 and later. In 3-day-old postnatal ovaries, it is expressed within oocytes in germ cell clusters and primordial follicles. In the adult, it is expressed only in small follicles, primary primordial and primary follicles. In testis, it is exclusively present in spermatogonia. Present at postnatal day 5, when spermatogonia stem cells differentiate into type A spermatogonia. Absent in Sertoli cells or spermatocytes (at protein level).1 Publication

Inductioni

Up-regulated in mice lacking Sohlh1.1 Publication

Gene expression databases

BgeeiQ9D489.
GenevisibleiQ9D489. MM.

Structurei

3D structure databases

ProteinModelPortaliQ9D489.
SMRiQ9D489. Positions 205-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini200 – 25152bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IUEE. Eukaryota.
ENOG410ZGUF. LUCA.
GeneTreeiENSGT00390000016050.
HOGENOMiHOG000070147.
HOVERGENiHBG103652.
InParanoidiQ9D489.
OMAiLPQHCNS.
OrthoDBiEOG7HB59C.
PhylomeDBiQ9D489.
TreeFamiTF336841.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032669. SOHLH2.
[Graphical view]
PANTHERiPTHR16223:SF16. PTHR16223:SF16. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRISTGEL GRRPGQGRVN LLLVGDATRY FLAGSMQKFF PSTAQITLTI
60 70 80 90 100
SNVKKVAVLL AANSFDIIFL KVTSTLTAEE QEAAKLIRSG KKKNTHLLFA
110 120 130 140 150
FVIPERLKGY VSEYGADISF SEPLTLEKVN TVIHYWKTYF TNTDMENTEL
160 170 180 190 200
PPECRLYFQT SRSELGGRFS TDMFLCSELL KNDARLGLKA PLSSLDKSKQ
210 220 230 240 250
ASFLHSTKEK LRRERIKSCC EQLRTLLPYV KGRKSDVASV IEATVDYVKQ
260 270 280 290 300
VRESLSPAIM AQVTEAIQNN RRFSKRQMPI ELFLPFSATS QREDAMLTSA
310 320 330 340 350
FSPVQEIQLL ADRGLNVYSM TAAGGALEGA VRGQPGSVSE SSIEDLYKTR
360 370 380 390 400
VPSTARSLNS FHSVKYTSGT VSPHEAAART NQNISTYLPP TAPSVSNFIP
410 420 430 440 450
QHCNAMLCPA RPTSPNCLCT PGHELAASSR AASASIFRGF RAASASDHQA
460
SQPPALPSPQ PHDSSYF
Length:467
Mass (Da):51,325
Last modified:January 15, 2008 - v2
Checksum:iAC29D2ACA2EDD0AE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 142RP → SV in BAB30389 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ086115 mRNA. Translation: AAY86077.1.
AK016704 mRNA. Translation: BAB30389.1.
BC120917 mRNA. Translation: AAI20918.1.
BC120918 mRNA. Translation: AAI20919.1.
CCDSiCCDS17358.1.
RefSeqiNP_083213.2. NM_028937.3.
UniGeneiMm.25514.

Genome annotation databases

EnsembliENSMUST00000029369; ENSMUSP00000029369; ENSMUSG00000027794.
GeneIDi74434.
KEGGimmu:74434.
UCSCiuc008pgi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ086115 mRNA. Translation: AAY86077.1.
AK016704 mRNA. Translation: BAB30389.1.
BC120917 mRNA. Translation: AAI20918.1.
BC120918 mRNA. Translation: AAI20919.1.
CCDSiCCDS17358.1.
RefSeqiNP_083213.2. NM_028937.3.
UniGeneiMm.25514.

3D structure databases

ProteinModelPortaliQ9D489.
SMRiQ9D489. Positions 205-252.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiQ9D489.
PaxDbiQ9D489.
PRIDEiQ9D489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029369; ENSMUSP00000029369; ENSMUSG00000027794.
GeneIDi74434.
KEGGimmu:74434.
UCSCiuc008pgi.2. mouse.

Organism-specific databases

CTDi54937.
MGIiMGI:1921684. Sohlh2.

Phylogenomic databases

eggNOGiENOG410IUEE. Eukaryota.
ENOG410ZGUF. LUCA.
GeneTreeiENSGT00390000016050.
HOGENOMiHOG000070147.
HOVERGENiHBG103652.
InParanoidiQ9D489.
OMAiLPQHCNS.
OrthoDBiEOG7HB59C.
PhylomeDBiQ9D489.
TreeFamiTF336841.

Miscellaneous databases

PROiQ9D489.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D489.
GenevisibleiQ9D489. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032669. SOHLH2.
[Graphical view]
PANTHERiPTHR16223:SF16. PTHR16223:SF16. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sohlh2 is a germ cell-specific bHLH transcription factor."
    Ballow D.J., Xin Y., Choi Y., Pangas S.A., Rajkovic A.
    Gene Expr. Patterns 6:1014-1018(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Sohlh1 is essential for spermatogonial differentiation."
    Ballow D., Meistrich M.L., Matzuk M., Rajkovic A.
    Dev. Biol. 294:161-167(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiSOLH2_MOUSE
AccessioniPrimary (citable) accession number: Q9D489
Secondary accession number(s): Q4JI40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: July 6, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.