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Protein

UV-stimulated scaffold protein A

Gene

Uvssa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Factor involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage. TC-NER allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Acts by promoting stabilization of ERCC6 by recruiting deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced degradation of ERCC6 by the proteasome. Interacts with the elongating form of RNA polymerase II (RNA pol IIo) and facilitates its ubiquitination at UV damage sites, leading to promote RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Not involved in processing oxidative damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
UV-stimulated scaffold protein A
Gene namesi
Name:Uvssa
Synonyms:Kiaa1530
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1918351. Uvssa.

Subcellular locationi

  • Chromosome By similarity

  • Note: Accumulates at UV DNA damage sites.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717UV-stimulated scaffold protein APRO_0000317283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei284 – 2841PhosphoserineBy similarity
Modified residuei291 – 2911PhosphoserineBy similarity

Post-translational modificationi

Monoubiquitinated: ubiquitination does not increase in response to UV.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9D479.

PTM databases

PhosphoSiteiQ9D479.

Expressioni

Gene expression databases

BgeeiQ9D479.
CleanExiMM_4933407H18RIK.
ExpressionAtlasiQ9D479. baseline and differential.
GenevestigatoriQ9D479.

Interactioni

Subunit structurei

Interacts with the elongating form of RNA polymerase II (RNA pol IIo). Interacts with ERCC6, ERCC8 and USP7 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000085170.

Structurei

3D structure databases

ProteinModelPortaliQ9D479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 145144VHS-likeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili169 – 19931Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi654 – 6629Poly-Lys

Sequence similaritiesi

Belongs to the UVSSA family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG313134.
GeneTreeiENSGT00390000000377.
HOGENOMiHOG000060117.
HOVERGENiHBG076421.
InParanoidiQ9D479.
OMAiRAVEGWN.
OrthoDBiEOG7X0VHQ.
PhylomeDBiQ9D479.
TreeFamiTF321660.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR018610. DUF2043.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF09740. DUF2043. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQKLSQLIE ELTTSGESQL NAQKMKELKK ICKSSEEQLS HAYRLLITQL
60 70 80 90 100
TQGHAEIRLS AFQIVDELFT RSHQFRMLLV SDFQEFLELT LGTDSDRPLP
110 120 130 140 150
PPREAAQRLR QAAMQAVEGW NEKFGQAYKK LALGYHFLKH TKKVDFRDIN
160 170 180 190 200
VRTVAERKRE EEKQKHLDKI HRESADRAKR EMEEMYDEIE CCLTEVENCF
210 220 230 240 250
KLLVPLDFVP CPEDKFFGEA SSMTEGYAPC PLSPDLATPR ESGLSGPQDE
260 270 280 290 300
EQPCCSKDLV ASAYHVGSVV GLKALPQTAM KDSSRDEDEP SDPDDFLRSH
310 320 330 340 350
GLGSHKYTLD VEVPSDGLKV QENEDNLAVL HAARDSLKLI QNKFLPTVCS
360 370 380 390 400
WVQRFTRAGT YSAHLKQAID LKMELELALK KYEELNIEPG RGQRSRTEAL
410 420 430 440 450
EDSEDEDQDF VEVPEKEGYE PRIPDHLRAE YGLEPKAPLK TLEKGTAVCK
460 470 480 490 500
LQERTRMRRE EEASDPTSAA AQMLRLQDCL SSPSPSSTRV LPGPEEAQKQ
510 520 530 540 550
AERARAPIVP FGVDLCYWGQ EQLTAGKILK SDSQHRFWKP SEVEEEVDSA
560 570 580 590 600
HVSEMLHSRH ITFSGTFEPV QHKCRALRPN GRLCERQDRL KCPFHGKIIP
610 620 630 640 650
RDDKGQPLNP EDRAREQRQQ LQRQQAHPDW QDPEFLKDVE AATGVDLGSS
660 670 680 690 700
RSSKKGKGKK KKHPNLTDLR ERTNTARARL EKKVFAKAAV QRVVAAMNQM
710
DQKKHEKFAN QFNYALK
Length:717
Mass (Da):81,759
Last modified:June 13, 2012 - v2
Checksum:iACC97A8DFAB47BB5
GO

Sequence cautioni

The sequence BAB30399.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC98194.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821D → G in AAI44921 (PubMed:15489334).Curated
Sequence conflicti397 – 44246Missing in BAC98194 (PubMed:14621295).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016722 mRNA. Translation: BAB30399.1. Different initiation.
AK156117 mRNA. Translation: BAE33591.1.
AC145072 Genomic DNA. No translation available.
CH466524 Genomic DNA. Translation: EDL37418.1.
BC061483 mRNA. Translation: AAH61483.1.
BC137803 mRNA. Translation: AAI37804.1.
BC144920 mRNA. Translation: AAI44921.1.
AK129384 Transcribed RNA. Translation: BAC98194.1. Different initiation.
CCDSiCCDS39064.1.
RefSeqiNP_001074570.1. NM_001081101.2.
XP_006504171.1. XM_006504108.2.
UniGeneiMm.132178.

Genome annotation databases

EnsembliENSMUST00000087864; ENSMUSP00000085170; ENSMUSG00000037355.
ENSMUST00000185821; ENSMUSP00000139989; ENSMUSG00000037355.
GeneIDi71101.
KEGGimmu:71101.
UCSCiuc008xas.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016722 mRNA. Translation: BAB30399.1. Different initiation.
AK156117 mRNA. Translation: BAE33591.1.
AC145072 Genomic DNA. No translation available.
CH466524 Genomic DNA. Translation: EDL37418.1.
BC061483 mRNA. Translation: AAH61483.1.
BC137803 mRNA. Translation: AAI37804.1.
BC144920 mRNA. Translation: AAI44921.1.
AK129384 Transcribed RNA. Translation: BAC98194.1. Different initiation.
CCDSiCCDS39064.1.
RefSeqiNP_001074570.1. NM_001081101.2.
XP_006504171.1. XM_006504108.2.
UniGeneiMm.132178.

3D structure databases

ProteinModelPortaliQ9D479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000085170.

PTM databases

PhosphoSiteiQ9D479.

Proteomic databases

PRIDEiQ9D479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087864; ENSMUSP00000085170; ENSMUSG00000037355.
ENSMUST00000185821; ENSMUSP00000139989; ENSMUSG00000037355.
GeneIDi71101.
KEGGimmu:71101.
UCSCiuc008xas.2. mouse.

Organism-specific databases

CTDi57654.
MGIiMGI:1918351. Uvssa.
RougeiSearch...

Phylogenomic databases

eggNOGiNOG313134.
GeneTreeiENSGT00390000000377.
HOGENOMiHOG000060117.
HOVERGENiHBG076421.
InParanoidiQ9D479.
OMAiRAVEGWN.
OrthoDBiEOG7X0VHQ.
PhylomeDBiQ9D479.
TreeFamiTF321660.

Miscellaneous databases

NextBioi333027.
PROiQ9D479.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D479.
CleanExiMM_4933407H18RIK.
ExpressionAtlasiQ9D479. baseline and differential.
GenevestigatoriQ9D479.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR018610. DUF2043.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF09740. DUF2043. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Spleen and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Limb.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 84-717.
    Tissue: Brain.

Entry informationi

Entry nameiUVSSA_MOUSE
AccessioniPrimary (citable) accession number: Q9D479
Secondary accession number(s): B2RQ84
, B7ZN03, Q3U1A8, Q6P7V8, Q6ZPN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 13, 2012
Last modified: May 27, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.