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Protein

Disks large-associated protein 1

Gene

Dlgap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the postsynaptic scaffold in neuronal cells.

GO - Biological processi

  • chemical synaptic transmission Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 1
Short name:
DAP-1
Alternative name(s):
Guanylate kinase-associated protein
PSD-95/SAP90-binding protein 1
SAP90/PSD-95-associated protein 1
Short name:
SAPAP1
Gene namesi
Name:Dlgap1
Synonyms:Gkap, Kiaa4162
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1346065. Dlgap1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • postsynaptic density Source: BHF-UCL
  • postsynaptic membrane Source: UniProtKB-KW
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001742891 – 992Disks large-associated protein 1Add BLAST992

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei372PhosphoserineCombined sources1
Modified residuei389PhosphoserineBy similarity1
Modified residuei418PhosphoserineCombined sources1
Modified residuei421PhosphoserineCombined sources1
Modified residuei425PhosphoserineCombined sources1
Modified residuei428PhosphoserineBy similarity1
Modified residuei437PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1
Modified residuei516PhosphoserineCombined sources1
Modified residuei578PhosphoserineCombined sources1
Modified residuei579PhosphothreonineCombined sources1
Modified residuei581PhosphoserineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei606PhosphothreonineCombined sources1
Modified residuei608PhosphoserineCombined sources1
Modified residuei611PhosphoserineBy similarity1
Modified residuei947PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D415.
PeptideAtlasiQ9D415.
PRIDEiQ9D415.

PTM databases

iPTMnetiQ9D415.
PhosphoSitePlusiQ9D415.

Expressioni

Tissue specificityi

Highest levels in the neocortex, part of the hippocampus, the granule cell layer of the cerebellum, the glomerular layer of the olfactory bulb, the inner plexiform layer of the retina, the ventral and dorsal horn of the spinal chord, the neuromuscular junction and the submandibular ganglion.1 Publication

Gene expression databases

BgeeiENSMUSG00000003279.
ExpressionAtlasiQ9D415. baseline and differential.
GenevisibleiQ9D415. MM.

Interactioni

Subunit structurei

Interacts with the guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.1 Publication

Protein-protein interaction databases

BioGridi230347. 2 interactors.
IntActiQ9D415. 4 interactors.
MINTiMINT-136157.
STRINGi10090.ENSMUSP00000122896.

Structurei

3D structure databases

ProteinModelPortaliQ9D415.
SMRiQ9D415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni665 – 676Interaction with DYL2By similarityAdd BLAST12
Regioni687 – 698Interaction with DYL2By similarityAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi990 – 992PDZ-bindingBy similarity3

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
GeneTreeiENSGT00550000074473.
HOVERGENiHBG018957.
InParanoidiQ9D415.
KOiK15008.
OMAiFMESYNT.
OrthoDBiEOG091G0J9O.
TreeFamiTF321382.

Family and domain databases

InterProiIPR030524. DLGAP1.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF7. PTHR12353:SF7. 2 hits.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D415-1) [UniParc]FASTAAdd to basket
Also known as: SAPAP1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGLSGSRSH HHGITCEAAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR
60 70 80 90 100
NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP
110 120 130 140 150
TNLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT
160 170 180 190 200
KSHSLEGPSK GSVNGGKASP DESQTLRYGK RSKSKERRSE SKARSNASNA
210 220 230 240 250
SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSASQ YFMEAYNTIS
260 270 280 290 300
EQAVKASRSN NDIKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK
310 320 330 340 350
ASVNMDQAMV KSEACQQERS CQYLQVPQDE WSGYTPRGKD DEIPCRRMRS
360 370 380 390 400
GSYIKAMGDE DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN
410 420 430 440 450
EHSPKLQIRS HSYLRAVSEV SINRSLDSLD PAGLLTSPKF RSRNESYMRA
460 470 480 490 500
MSTISQVSEM EVNGQFESVC ESVFSELESQ AVEALDLPLP GCFRMRSHSY
510 520 530 540 550
VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK LSSAVEVSSC
560 570 580 590 600
ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS
610 620 630 640 650
GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV
660 670 680 690 700
TTEDRKKDFK KNRCLSIGIQ VDDAEEPEKM AESKTSNKFQ SVGVQVEEEK
710 720 730 740 750
CFRRFTRSNS VTTAVQADLD FHDNLENSLE SIEDNSCPGP MARQFSRDAS
760 770 780 790 800
TSTVSIQGSG NHYHACAADD DFDTDFDPSI LPPPDPWIDS ITEDPLEAVQ
810 820 830 840 850
RSVCHRDGHW FLKLLQAERD RMEGWCKLME REERENNLPE DILGKIRTAV
860 870 880 890 900
GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS
910 920 930 940 950
MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES
960 970 980 990
SQRQEARKRL MAAKRAASVR QNSATESAES IEIYIPEAQT RL
Length:992
Mass (Da):110,374
Last modified:July 19, 2005 - v3
Checksum:i9962C8B56A33EE13
GO
Isoform 2 (identifier: Q9D415-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     537-546: Missing.

Note: No experimental confirmation available.
Show »
Length:982
Mass (Da):109,390
Checksum:i9518AAB72BB13EE9
GO
Isoform 3 (identifier: Q9D415-3) [UniParc]FASTAAdd to basket
Also known as: GKAP1a

The sequence of this isoform differs from the canonical sequence as follows:
     547-574: Missing.

Show »
Length:964
Mass (Da):107,331
Checksum:iB45BBAFF2C4E8740
GO
Isoform 4 (identifier: Q9D415-4) [UniParc]FASTAAdd to basket
Also known as: GKAP1b

The sequence of this isoform differs from the canonical sequence as follows:
     537-546: Missing.
     924-944: ERRAPPPVPKKPAKGPAPLIR → VEQCRFCMVHLKPCTNAGQSK
     945-992: Missing.

Show »
Length:934
Mass (Da):104,064
Checksum:i224A365837BCE785
GO
Isoform 5 (identifier: Q9D415-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     538-538: V → E
     539-992: Missing.

Note: No experimental confirmation available.
Show »
Length:538
Mass (Da):59,879
Checksum:iA562146ADAE2C929
GO
Isoform 6 (identifier: Q9D415-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     398-992: Missing.

Note: No experimental confirmation available.
Show »
Length:397
Mass (Da):44,247
Checksum:i63EB6CC30E996F60
GO

Sequence cautioni

The sequence BAD90519 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti383L → P in BAD90519 (Ref. 3) Curated1
Sequence conflicti397K → R in AAP70755 (PubMed:15024750).Curated1
Sequence conflicti431P → H in BAC27391 (PubMed:16141072).Curated1
Sequence conflicti752S → F in BAC28619 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015408398 – 992Missing in isoform 6. 1 PublicationAdd BLAST595
Alternative sequenceiVSP_015409537 – 546Missing in isoform 2 and isoform 4. 3 Publications10
Alternative sequenceiVSP_015410538V → E in isoform 5. 1 Publication1
Alternative sequenceiVSP_015411539 – 992Missing in isoform 5. 1 PublicationAdd BLAST454
Alternative sequenceiVSP_015412547 – 574Missing in isoform 3. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_015413924 – 944ERRAP…APLIR → VEQCRFCMVHLKPCTNAGQS K in isoform 4. 2 PublicationsAdd BLAST21
Alternative sequenceiVSP_015414945 – 992Missing in isoform 4. 2 PublicationsAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY243846 mRNA. Translation: AAP70755.2.
AK031410 mRNA. Translation: BAC27391.1.
AK033901 mRNA. Translation: BAC28508.1.
AK034182 mRNA. Translation: BAC28619.1.
AK220515 mRNA. Translation: BAD90519.1. Different initiation.
BC062120 mRNA. Translation: AAH62120.1.
BC094369 mRNA. Translation: AAH94369.1.
CCDSiCCDS28953.1. [Q9D415-1]
RefSeqiNP_081988.3. NM_027712.3. [Q9D415-4]
NP_808307.2. NM_177639.6. [Q9D415-1]
XP_006524236.1. XM_006524173.2. [Q9D415-1]
XP_006524239.1. XM_006524176.2. [Q9D415-3]
XP_011244709.1. XM_011246407.2. [Q9D415-1]
UniGeneiMm.311840.
Mm.367369.

Genome annotation databases

EnsembliENSMUST00000060072; ENSMUSP00000052858; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000133983; ENSMUSP00000116716; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000135938; ENSMUSP00000118497; ENSMUSG00000003279. [Q9D415-3]
ENSMUST00000155016; ENSMUSP00000122896; ENSMUSG00000003279. [Q9D415-1]
GeneIDi224997.
KEGGimmu:224997.
UCSCiuc008dky.1. mouse. [Q9D415-6]
uc008dla.1. mouse. [Q9D415-5]
uc008dle.2. mouse. [Q9D415-1]
uc008dlf.2. mouse. [Q9D415-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY243846 mRNA. Translation: AAP70755.2.
AK031410 mRNA. Translation: BAC27391.1.
AK033901 mRNA. Translation: BAC28508.1.
AK034182 mRNA. Translation: BAC28619.1.
AK220515 mRNA. Translation: BAD90519.1. Different initiation.
BC062120 mRNA. Translation: AAH62120.1.
BC094369 mRNA. Translation: AAH94369.1.
CCDSiCCDS28953.1. [Q9D415-1]
RefSeqiNP_081988.3. NM_027712.3. [Q9D415-4]
NP_808307.2. NM_177639.6. [Q9D415-1]
XP_006524236.1. XM_006524173.2. [Q9D415-1]
XP_006524239.1. XM_006524176.2. [Q9D415-3]
XP_011244709.1. XM_011246407.2. [Q9D415-1]
UniGeneiMm.311840.
Mm.367369.

3D structure databases

ProteinModelPortaliQ9D415.
SMRiQ9D415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230347. 2 interactors.
IntActiQ9D415. 4 interactors.
MINTiMINT-136157.
STRINGi10090.ENSMUSP00000122896.

PTM databases

iPTMnetiQ9D415.
PhosphoSitePlusiQ9D415.

Proteomic databases

PaxDbiQ9D415.
PeptideAtlasiQ9D415.
PRIDEiQ9D415.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060072; ENSMUSP00000052858; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000133983; ENSMUSP00000116716; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000135938; ENSMUSP00000118497; ENSMUSG00000003279. [Q9D415-3]
ENSMUST00000155016; ENSMUSP00000122896; ENSMUSG00000003279. [Q9D415-1]
GeneIDi224997.
KEGGimmu:224997.
UCSCiuc008dky.1. mouse. [Q9D415-6]
uc008dla.1. mouse. [Q9D415-5]
uc008dle.2. mouse. [Q9D415-1]
uc008dlf.2. mouse. [Q9D415-4]

Organism-specific databases

CTDi9229.
MGIiMGI:1346065. Dlgap1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
GeneTreeiENSGT00550000074473.
HOVERGENiHBG018957.
InParanoidiQ9D415.
KOiK15008.
OMAiFMESYNT.
OrthoDBiEOG091G0J9O.
TreeFamiTF321382.

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

ChiTaRSiDlgap1. mouse.
PROiQ9D415.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003279.
ExpressionAtlasiQ9D415. baseline and differential.
GenevisibleiQ9D415. MM.

Family and domain databases

InterProiIPR030524. DLGAP1.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF7. PTHR12353:SF7. 2 hits.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLGP1_MOUSE
AccessioniPrimary (citable) accession number: Q9D415
Secondary accession number(s): Q52KF6
, Q5DTK5, Q6P6N4, Q6XBF4, Q8BZL7, Q8BZQ1, Q8C0G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.