Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9D415

- DLGP1_MOUSE

UniProt

Q9D415 - DLGP1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Disks large-associated protein 1

Gene

Dlgap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of the postsynaptic scaffold in neuronal cells.

GO - Biological processi

  1. synaptic transmission Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 1
Short name:
DAP-1
Alternative name(s):
Guanylate kinase-associated protein
PSD-95/SAP90-binding protein 1
SAP90/PSD-95-associated protein 1
Short name:
SAPAP1
Gene namesi
Name:Dlgap1
Synonyms:Gkap, Kiaa4162
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1346065. Dlgap1.

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Cell junctionsynapse 1 Publication

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. neuronal postsynaptic density Source: BHF-UCL
  3. postsynaptic membrane Source: UniProtKB-KW
  4. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 992992Disks large-associated protein 1PRO_0000174289Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei362 – 3621Phosphoserine1 Publication
Modified residuei509 – 5091Phosphoserine1 Publication
Modified residuei516 – 5161Phosphoserine1 Publication
Modified residuei947 – 9471Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9D415.
PaxDbiQ9D415.
PRIDEiQ9D415.

PTM databases

PhosphoSiteiQ9D415.

Expressioni

Tissue specificityi

Highest levels in the neocortex, part of the hippocampus, the granule cell layer of the cerebellum, the glomerular layer of the olfactory bulb, the inner plexiform layer of the retina, the ventral and dorsal horn of the spinal chord, the neuromuscular junction and the submandibular ganglion.1 Publication

Gene expression databases

BgeeiQ9D415.
ExpressionAtlasiQ9D415. baseline and differential.
GenevestigatoriQ9D415.

Interactioni

Subunit structurei

Interacts with the guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.1 Publication

Protein-protein interaction databases

BioGridi230347. 2 interactions.
IntActiQ9D415. 4 interactions.
MINTiMINT-136157.

Structurei

3D structure databases

ProteinModelPortaliQ9D415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni665 – 67612Interaction with DYL2By similarityAdd
BLAST
Regioni687 – 69812Interaction with DYL2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi990 – 9923PDZ-bindingBy similarity

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Phylogenomic databases

eggNOGiNOG277836.
GeneTreeiENSGT00550000074473.
HOVERGENiHBG018957.
InParanoidiQ9D415.
KOiK15008.
OMAiAVEVSSC.
OrthoDBiEOG75QR37.
TreeFamiTF321382.

Family and domain databases

InterProiIPR005026. GKAP.
[Graphical view]
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9D415-1) [UniParc]FASTAAdd to Basket

Also known as: SAPAP1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGLSGSRSH HHGITCEAAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR
60 70 80 90 100
NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP
110 120 130 140 150
TNLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT
160 170 180 190 200
KSHSLEGPSK GSVNGGKASP DESQTLRYGK RSKSKERRSE SKARSNASNA
210 220 230 240 250
SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSASQ YFMEAYNTIS
260 270 280 290 300
EQAVKASRSN NDIKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK
310 320 330 340 350
ASVNMDQAMV KSEACQQERS CQYLQVPQDE WSGYTPRGKD DEIPCRRMRS
360 370 380 390 400
GSYIKAMGDE DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN
410 420 430 440 450
EHSPKLQIRS HSYLRAVSEV SINRSLDSLD PAGLLTSPKF RSRNESYMRA
460 470 480 490 500
MSTISQVSEM EVNGQFESVC ESVFSELESQ AVEALDLPLP GCFRMRSHSY
510 520 530 540 550
VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK LSSAVEVSSC
560 570 580 590 600
ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS
610 620 630 640 650
GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV
660 670 680 690 700
TTEDRKKDFK KNRCLSIGIQ VDDAEEPEKM AESKTSNKFQ SVGVQVEEEK
710 720 730 740 750
CFRRFTRSNS VTTAVQADLD FHDNLENSLE SIEDNSCPGP MARQFSRDAS
760 770 780 790 800
TSTVSIQGSG NHYHACAADD DFDTDFDPSI LPPPDPWIDS ITEDPLEAVQ
810 820 830 840 850
RSVCHRDGHW FLKLLQAERD RMEGWCKLME REERENNLPE DILGKIRTAV
860 870 880 890 900
GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS
910 920 930 940 950
MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES
960 970 980 990
SQRQEARKRL MAAKRAASVR QNSATESAES IEIYIPEAQT RL
Length:992
Mass (Da):110,374
Last modified:July 19, 2005 - v3
Checksum:i9962C8B56A33EE13
GO
Isoform 2 (identifier: Q9D415-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     537-546: Missing.

Note: No experimental confirmation available.

Show »
Length:982
Mass (Da):109,390
Checksum:i9518AAB72BB13EE9
GO
Isoform 3 (identifier: Q9D415-3) [UniParc]FASTAAdd to Basket

Also known as: GKAP1a

The sequence of this isoform differs from the canonical sequence as follows:
     547-574: Missing.

Show »
Length:964
Mass (Da):107,331
Checksum:iB45BBAFF2C4E8740
GO
Isoform 4 (identifier: Q9D415-4) [UniParc]FASTAAdd to Basket

Also known as: GKAP1b

The sequence of this isoform differs from the canonical sequence as follows:
     537-546: Missing.
     924-944: ERRAPPPVPKKPAKGPAPLIR → VEQCRFCMVHLKPCTNAGQSK
     945-992: Missing.

Show »
Length:934
Mass (Da):104,064
Checksum:i224A365837BCE785
GO
Isoform 5 (identifier: Q9D415-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     538-538: V → E
     539-992: Missing.

Note: No experimental confirmation available.

Show »
Length:538
Mass (Da):59,879
Checksum:iA562146ADAE2C929
GO
Isoform 6 (identifier: Q9D415-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     398-992: Missing.

Note: No experimental confirmation available.

Show »
Length:397
Mass (Da):44,247
Checksum:i63EB6CC30E996F60
GO

Sequence cautioni

The sequence BAD90519.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti383 – 3831L → P in BAD90519. 1 PublicationCurated
Sequence conflicti397 – 3971K → R in AAP70755. (PubMed:15024750)Curated
Sequence conflicti431 – 4311P → H in BAC27391. (PubMed:16141072)Curated
Sequence conflicti752 – 7521S → F in BAC28619. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei398 – 992595Missing in isoform 6. 1 PublicationVSP_015408Add
BLAST
Alternative sequencei537 – 54610Missing in isoform 2 and isoform 4. 3 PublicationsVSP_015409
Alternative sequencei538 – 5381V → E in isoform 5. 1 PublicationVSP_015410
Alternative sequencei539 – 992454Missing in isoform 5. 1 PublicationVSP_015411Add
BLAST
Alternative sequencei547 – 57428Missing in isoform 3. 1 PublicationVSP_015412Add
BLAST
Alternative sequencei924 – 94421ERRAP…APLIR → VEQCRFCMVHLKPCTNAGQS K in isoform 4. 2 PublicationsVSP_015413Add
BLAST
Alternative sequencei945 – 99248Missing in isoform 4. 2 PublicationsVSP_015414Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY243846 mRNA. Translation: AAP70755.2.
AK031410 mRNA. Translation: BAC27391.1.
AK033901 mRNA. Translation: BAC28508.1.
AK034182 mRNA. Translation: BAC28619.1.
AK220515 mRNA. Translation: BAD90519.1. Different initiation.
BC062120 mRNA. Translation: AAH62120.1.
BC094369 mRNA. Translation: AAH94369.1.
CCDSiCCDS28953.1. [Q9D415-1]
RefSeqiNP_081988.3. NM_027712.3. [Q9D415-4]
NP_808307.2. NM_177639.6. [Q9D415-1]
XP_006524236.1. XM_006524173.1. [Q9D415-1]
XP_006524239.1. XM_006524176.1. [Q9D415-3]
UniGeneiMm.311840.
Mm.367369.

Genome annotation databases

EnsembliENSMUST00000060072; ENSMUSP00000052858; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000133983; ENSMUSP00000116716; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000135938; ENSMUSP00000118497; ENSMUSG00000003279. [Q9D415-3]
ENSMUST00000155016; ENSMUSP00000122896; ENSMUSG00000003279. [Q9D415-1]
GeneIDi224997.
KEGGimmu:224997.
UCSCiuc008dky.1. mouse. [Q9D415-6]
uc008dla.1. mouse. [Q9D415-5]
uc008dle.2. mouse. [Q9D415-1]
uc008dlf.2. mouse. [Q9D415-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY243846 mRNA. Translation: AAP70755.2 .
AK031410 mRNA. Translation: BAC27391.1 .
AK033901 mRNA. Translation: BAC28508.1 .
AK034182 mRNA. Translation: BAC28619.1 .
AK220515 mRNA. Translation: BAD90519.1 . Different initiation.
BC062120 mRNA. Translation: AAH62120.1 .
BC094369 mRNA. Translation: AAH94369.1 .
CCDSi CCDS28953.1. [Q9D415-1 ]
RefSeqi NP_081988.3. NM_027712.3. [Q9D415-4 ]
NP_808307.2. NM_177639.6. [Q9D415-1 ]
XP_006524236.1. XM_006524173.1. [Q9D415-1 ]
XP_006524239.1. XM_006524176.1. [Q9D415-3 ]
UniGenei Mm.311840.
Mm.367369.

3D structure databases

ProteinModelPortali Q9D415.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230347. 2 interactions.
IntActi Q9D415. 4 interactions.
MINTi MINT-136157.

PTM databases

PhosphoSitei Q9D415.

Proteomic databases

MaxQBi Q9D415.
PaxDbi Q9D415.
PRIDEi Q9D415.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000060072 ; ENSMUSP00000052858 ; ENSMUSG00000003279 . [Q9D415-2 ]
ENSMUST00000133983 ; ENSMUSP00000116716 ; ENSMUSG00000003279 . [Q9D415-2 ]
ENSMUST00000135938 ; ENSMUSP00000118497 ; ENSMUSG00000003279 . [Q9D415-3 ]
ENSMUST00000155016 ; ENSMUSP00000122896 ; ENSMUSG00000003279 . [Q9D415-1 ]
GeneIDi 224997.
KEGGi mmu:224997.
UCSCi uc008dky.1. mouse. [Q9D415-6 ]
uc008dla.1. mouse. [Q9D415-5 ]
uc008dle.2. mouse. [Q9D415-1 ]
uc008dlf.2. mouse. [Q9D415-4 ]

Organism-specific databases

CTDi 9229.
MGIi MGI:1346065. Dlgap1.
Rougei Search...

Phylogenomic databases

eggNOGi NOG277836.
GeneTreei ENSGT00550000074473.
HOVERGENi HBG018957.
InParanoidi Q9D415.
KOi K15008.
OMAi AVEVSSC.
OrthoDBi EOG75QR37.
TreeFami TF321382.

Miscellaneous databases

NextBioi 377488.
PROi Q9D415.
SOURCEi Search...

Gene expression databases

Bgeei Q9D415.
ExpressionAtlasi Q9D415. baseline and differential.
Genevestigatori Q9D415.

Family and domain databases

InterProi IPR005026. GKAP.
[Graphical view ]
Pfami PF03359. GKAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse."
    Welch J.M., Wang D., Feng G.
    J. Comp. Neurol. 472:24-39(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
    Strain: ICR.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 6), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 660-992 (ISOFORM 4).
    Strain: C57BL/6J.
    Tissue: Diencephalon and Testis.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Fetal brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 210-992 (ISOFORM 4).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362; SER-509; SER-516 AND SER-947, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  7. "SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
    Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
    Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHANK3, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDLGP1_MOUSE
AccessioniPrimary (citable) accession number: Q9D415
Secondary accession number(s): Q52KF6
, Q5DTK5, Q6P6N4, Q6XBF4, Q8BZL7, Q8BZQ1, Q8C0G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 19, 2005
Last modified: October 29, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3