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Q9D415

- DLGP1_MOUSE

UniProt

Q9D415 - DLGP1_MOUSE

Protein

Disks large-associated protein 1

Gene

Dlgap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Part of the postsynaptic scaffold in neuronal cells.

    GO - Biological processi

    1. synaptic transmission Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disks large-associated protein 1
    Short name:
    DAP-1
    Alternative name(s):
    Guanylate kinase-associated protein
    PSD-95/SAP90-binding protein 1
    SAP90/PSD-95-associated protein 1
    Short name:
    SAPAP1
    Gene namesi
    Name:Dlgap1
    Synonyms:Gkap, Kiaa4162
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1346065. Dlgap1.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Cell junctionsynapse 1 Publication

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. neuronal postsynaptic density Source: BHF-UCL
    3. postsynaptic membrane Source: UniProtKB-KW
    4. synapse Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 992992Disks large-associated protein 1PRO_0000174289Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei362 – 3621Phosphoserine1 Publication
    Modified residuei509 – 5091Phosphoserine1 Publication
    Modified residuei516 – 5161Phosphoserine1 Publication
    Modified residuei947 – 9471Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9D415.
    PaxDbiQ9D415.
    PRIDEiQ9D415.

    PTM databases

    PhosphoSiteiQ9D415.

    Expressioni

    Tissue specificityi

    Highest levels in the neocortex, part of the hippocampus, the granule cell layer of the cerebellum, the glomerular layer of the olfactory bulb, the inner plexiform layer of the retina, the ventral and dorsal horn of the spinal chord, the neuromuscular junction and the submandibular ganglion.1 Publication

    Gene expression databases

    BgeeiQ9D415.
    GenevestigatoriQ9D415.

    Interactioni

    Subunit structurei

    Interacts with the guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.1 Publication

    Protein-protein interaction databases

    BioGridi230347. 2 interactions.
    IntActiQ9D415. 4 interactions.
    MINTiMINT-136157.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D415.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni665 – 67612Interaction with DYL2By similarityAdd
    BLAST
    Regioni687 – 69812Interaction with DYL2By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi990 – 9923PDZ-bindingBy similarity

    Sequence similaritiesi

    Belongs to the SAPAP family.Curated

    Phylogenomic databases

    eggNOGiNOG277836.
    GeneTreeiENSGT00550000074473.
    HOVERGENiHBG018957.
    InParanoidiQ9D415.
    KOiK15008.
    OMAiAVEVSSC.
    OrthoDBiEOG75QR37.
    TreeFamiTF321382.

    Family and domain databases

    InterProiIPR005026. GKAP.
    [Graphical view]
    PfamiPF03359. GKAP. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9D415-1) [UniParc]FASTAAdd to Basket

    Also known as: SAPAP1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKGLSGSRSH HHGITCEAAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR    50
    NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP 100
    TNLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT 150
    KSHSLEGPSK GSVNGGKASP DESQTLRYGK RSKSKERRSE SKARSNASNA 200
    SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSASQ YFMEAYNTIS 250
    EQAVKASRSN NDIKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK 300
    ASVNMDQAMV KSEACQQERS CQYLQVPQDE WSGYTPRGKD DEIPCRRMRS 350
    GSYIKAMGDE DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN 400
    EHSPKLQIRS HSYLRAVSEV SINRSLDSLD PAGLLTSPKF RSRNESYMRA 450
    MSTISQVSEM EVNGQFESVC ESVFSELESQ AVEALDLPLP GCFRMRSHSY 500
    VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK LSSAVEVSSC 550
    ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS 600
    GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV 650
    TTEDRKKDFK KNRCLSIGIQ VDDAEEPEKM AESKTSNKFQ SVGVQVEEEK 700
    CFRRFTRSNS VTTAVQADLD FHDNLENSLE SIEDNSCPGP MARQFSRDAS 750
    TSTVSIQGSG NHYHACAADD DFDTDFDPSI LPPPDPWIDS ITEDPLEAVQ 800
    RSVCHRDGHW FLKLLQAERD RMEGWCKLME REERENNLPE DILGKIRTAV 850
    GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS 900
    MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES 950
    SQRQEARKRL MAAKRAASVR QNSATESAES IEIYIPEAQT RL 992
    Length:992
    Mass (Da):110,374
    Last modified:July 19, 2005 - v3
    Checksum:i9962C8B56A33EE13
    GO
    Isoform 2 (identifier: Q9D415-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         537-546: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:982
    Mass (Da):109,390
    Checksum:i9518AAB72BB13EE9
    GO
    Isoform 3 (identifier: Q9D415-3) [UniParc]FASTAAdd to Basket

    Also known as: GKAP1a

    The sequence of this isoform differs from the canonical sequence as follows:
         547-574: Missing.

    Show »
    Length:964
    Mass (Da):107,331
    Checksum:iB45BBAFF2C4E8740
    GO
    Isoform 4 (identifier: Q9D415-4) [UniParc]FASTAAdd to Basket

    Also known as: GKAP1b

    The sequence of this isoform differs from the canonical sequence as follows:
         537-546: Missing.
         924-944: ERRAPPPVPKKPAKGPAPLIR → VEQCRFCMVHLKPCTNAGQSK
         945-992: Missing.

    Show »
    Length:934
    Mass (Da):104,064
    Checksum:i224A365837BCE785
    GO
    Isoform 5 (identifier: Q9D415-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         538-538: V → E
         539-992: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:538
    Mass (Da):59,879
    Checksum:iA562146ADAE2C929
    GO
    Isoform 6 (identifier: Q9D415-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         398-992: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:397
    Mass (Da):44,247
    Checksum:i63EB6CC30E996F60
    GO

    Sequence cautioni

    The sequence BAD90519.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti383 – 3831L → P in BAD90519. 1 PublicationCurated
    Sequence conflicti397 – 3971K → R in AAP70755. (PubMed:15024750)Curated
    Sequence conflicti431 – 4311P → H in BAC27391. (PubMed:16141072)Curated
    Sequence conflicti752 – 7521S → F in BAC28619. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei398 – 992595Missing in isoform 6. 1 PublicationVSP_015408Add
    BLAST
    Alternative sequencei537 – 54610Missing in isoform 2 and isoform 4. 3 PublicationsVSP_015409
    Alternative sequencei538 – 5381V → E in isoform 5. 1 PublicationVSP_015410
    Alternative sequencei539 – 992454Missing in isoform 5. 1 PublicationVSP_015411Add
    BLAST
    Alternative sequencei547 – 57428Missing in isoform 3. 1 PublicationVSP_015412Add
    BLAST
    Alternative sequencei924 – 94421ERRAP…APLIR → VEQCRFCMVHLKPCTNAGQS K in isoform 4. 2 PublicationsVSP_015413Add
    BLAST
    Alternative sequencei945 – 99248Missing in isoform 4. 2 PublicationsVSP_015414Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY243846 mRNA. Translation: AAP70755.2.
    AK031410 mRNA. Translation: BAC27391.1.
    AK033901 mRNA. Translation: BAC28508.1.
    AK034182 mRNA. Translation: BAC28619.1.
    AK220515 mRNA. Translation: BAD90519.1. Different initiation.
    BC062120 mRNA. Translation: AAH62120.1.
    BC094369 mRNA. Translation: AAH94369.1.
    CCDSiCCDS28953.1. [Q9D415-1]
    RefSeqiNP_081988.3. NM_027712.3. [Q9D415-4]
    NP_808307.2. NM_177639.6. [Q9D415-1]
    XP_006524236.1. XM_006524173.1. [Q9D415-1]
    XP_006524239.1. XM_006524176.1. [Q9D415-3]
    UniGeneiMm.311840.
    Mm.367369.

    Genome annotation databases

    EnsembliENSMUST00000060072; ENSMUSP00000052858; ENSMUSG00000003279. [Q9D415-2]
    ENSMUST00000133983; ENSMUSP00000116716; ENSMUSG00000003279. [Q9D415-2]
    ENSMUST00000135938; ENSMUSP00000118497; ENSMUSG00000003279. [Q9D415-3]
    ENSMUST00000155016; ENSMUSP00000122896; ENSMUSG00000003279. [Q9D415-1]
    GeneIDi224997.
    KEGGimmu:224997.
    UCSCiuc008dky.1. mouse. [Q9D415-6]
    uc008dla.1. mouse. [Q9D415-5]
    uc008dle.2. mouse. [Q9D415-1]
    uc008dlf.2. mouse. [Q9D415-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY243846 mRNA. Translation: AAP70755.2 .
    AK031410 mRNA. Translation: BAC27391.1 .
    AK033901 mRNA. Translation: BAC28508.1 .
    AK034182 mRNA. Translation: BAC28619.1 .
    AK220515 mRNA. Translation: BAD90519.1 . Different initiation.
    BC062120 mRNA. Translation: AAH62120.1 .
    BC094369 mRNA. Translation: AAH94369.1 .
    CCDSi CCDS28953.1. [Q9D415-1 ]
    RefSeqi NP_081988.3. NM_027712.3. [Q9D415-4 ]
    NP_808307.2. NM_177639.6. [Q9D415-1 ]
    XP_006524236.1. XM_006524173.1. [Q9D415-1 ]
    XP_006524239.1. XM_006524176.1. [Q9D415-3 ]
    UniGenei Mm.311840.
    Mm.367369.

    3D structure databases

    ProteinModelPortali Q9D415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230347. 2 interactions.
    IntActi Q9D415. 4 interactions.
    MINTi MINT-136157.

    PTM databases

    PhosphoSitei Q9D415.

    Proteomic databases

    MaxQBi Q9D415.
    PaxDbi Q9D415.
    PRIDEi Q9D415.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000060072 ; ENSMUSP00000052858 ; ENSMUSG00000003279 . [Q9D415-2 ]
    ENSMUST00000133983 ; ENSMUSP00000116716 ; ENSMUSG00000003279 . [Q9D415-2 ]
    ENSMUST00000135938 ; ENSMUSP00000118497 ; ENSMUSG00000003279 . [Q9D415-3 ]
    ENSMUST00000155016 ; ENSMUSP00000122896 ; ENSMUSG00000003279 . [Q9D415-1 ]
    GeneIDi 224997.
    KEGGi mmu:224997.
    UCSCi uc008dky.1. mouse. [Q9D415-6 ]
    uc008dla.1. mouse. [Q9D415-5 ]
    uc008dle.2. mouse. [Q9D415-1 ]
    uc008dlf.2. mouse. [Q9D415-4 ]

    Organism-specific databases

    CTDi 9229.
    MGIi MGI:1346065. Dlgap1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG277836.
    GeneTreei ENSGT00550000074473.
    HOVERGENi HBG018957.
    InParanoidi Q9D415.
    KOi K15008.
    OMAi AVEVSSC.
    OrthoDBi EOG75QR37.
    TreeFami TF321382.

    Miscellaneous databases

    NextBioi 377488.
    PROi Q9D415.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D415.
    Genevestigatori Q9D415.

    Family and domain databases

    InterProi IPR005026. GKAP.
    [Graphical view ]
    Pfami PF03359. GKAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse."
      Welch J.M., Wang D., Feng G.
      J. Comp. Neurol. 472:24-39(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
      Strain: ICR.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 6), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 660-992 (ISOFORM 4).
      Strain: C57BL/6J.
      Tissue: Diencephalon and Testis.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Fetal brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 210-992 (ISOFORM 4).
      Strain: C57BL/6.
      Tissue: Brain.
    5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362; SER-509; SER-516 AND SER-947, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    7. "SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
      Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
      Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHANK3, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDLGP1_MOUSE
    AccessioniPrimary (citable) accession number: Q9D415
    Secondary accession number(s): Q52KF6
    , Q5DTK5, Q6P6N4, Q6XBF4, Q8BZL7, Q8BZQ1, Q8C0G0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3