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Q9D415 (DLGP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disks large-associated protein 1

Short name=DAP-1
Alternative name(s):
Guanylate kinase-associated protein
PSD-95/SAP90-binding protein 1
SAP90/PSD-95-associated protein 1
Short name=SAPAP1
Gene names
Name:Dlgap1
Synonyms:Gkap, Kiaa4162
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length992 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the postsynaptic scaffold in neuronal cells.

Subunit structure

Interacts with the guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1. Ref.7

Subcellular location

Cell membrane; Peripheral membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Cell junctionsynapse Ref.7.

Tissue specificity

Highest levels in the neocortex, part of the hippocampus, the granule cell layer of the cerebellum, the glomerular layer of the olfactory bulb, the inner plexiform layer of the retina, the ventral and dorsal horn of the spinal chord, the neuromuscular junction and the submandibular ganglion. Ref.1

Sequence similarities

Belongs to the SAPAP family.

Sequence caution

The sequence BAD90519.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9D415-1)

Also known as: SAPAP1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9D415-2)

The sequence of this isoform differs from the canonical sequence as follows:
     537-546: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9D415-3)

Also known as: GKAP1a;

The sequence of this isoform differs from the canonical sequence as follows:
     547-574: Missing.
Isoform 4 (identifier: Q9D415-4)

Also known as: GKAP1b;

The sequence of this isoform differs from the canonical sequence as follows:
     537-546: Missing.
     924-944: ERRAPPPVPKKPAKGPAPLIR → VEQCRFCMVHLKPCTNAGQSK
     945-992: Missing.
Isoform 5 (identifier: Q9D415-5)

The sequence of this isoform differs from the canonical sequence as follows:
     538-538: V → E
     539-992: Missing.
Note: No experimental confirmation available.
Isoform 6 (identifier: Q9D415-6)

The sequence of this isoform differs from the canonical sequence as follows:
     398-992: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 992992Disks large-associated protein 1
PRO_0000174289

Regions

Region665 – 67612Interaction with DYL2 By similarity
Region687 – 69812Interaction with DYL2 By similarity
Motif990 – 9923PDZ-binding By similarity

Amino acid modifications

Modified residue3621Phosphoserine Ref.5
Modified residue5091Phosphoserine Ref.5
Modified residue5161Phosphoserine Ref.5
Modified residue9471Phosphoserine Ref.5

Natural variations

Alternative sequence398 – 992595Missing in isoform 6.
VSP_015408
Alternative sequence537 – 54610Missing in isoform 2 and isoform 4.
VSP_015409
Alternative sequence5381V → E in isoform 5.
VSP_015410
Alternative sequence539 – 992454Missing in isoform 5.
VSP_015411
Alternative sequence547 – 57428Missing in isoform 3.
VSP_015412
Alternative sequence924 – 94421ERRAP…APLIR → VEQCRFCMVHLKPCTNAGQS K in isoform 4.
VSP_015413
Alternative sequence945 – 99248Missing in isoform 4.
VSP_015414

Experimental info

Sequence conflict3831L → P in BAD90519. Ref.3
Sequence conflict3971K → R in AAP70755. Ref.1
Sequence conflict4311P → H in BAC27391. Ref.2
Sequence conflict7521S → F in BAC28619. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SAPAP1) [UniParc].

Last modified July 19, 2005. Version 3.
Checksum: 9962C8B56A33EE13

FASTA992110,374
        10         20         30         40         50         60 
MKGLSGSRSH HHGITCEAAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR NSFQAECVGP 

        70         80         90        100        110        120 
FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP TNLLDQFERQ LPLSRDGYHT 

       130        140        150        160        170        180 
LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT KSHSLEGPSK GSVNGGKASP DESQTLRYGK 

       190        200        210        220        230        240 
RSKSKERRSE SKARSNASNA SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSASQ 

       250        260        270        280        290        300 
YFMEAYNTIS EQAVKASRSN NDIKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK 

       310        320        330        340        350        360 
ASVNMDQAMV KSEACQQERS CQYLQVPQDE WSGYTPRGKD DEIPCRRMRS GSYIKAMGDE 

       370        380        390        400        410        420 
DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN EHSPKLQIRS HSYLRAVSEV 

       430        440        450        460        470        480 
SINRSLDSLD PAGLLTSPKF RSRNESYMRA MSTISQVSEM EVNGQFESVC ESVFSELESQ 

       490        500        510        520        530        540 
AVEALDLPLP GCFRMRSHSY VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK 

       550        560        570        580        590        600 
LSSAVEVSSC ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS 

       610        620        630        640        650        660 
GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV TTEDRKKDFK 

       670        680        690        700        710        720 
KNRCLSIGIQ VDDAEEPEKM AESKTSNKFQ SVGVQVEEEK CFRRFTRSNS VTTAVQADLD 

       730        740        750        760        770        780 
FHDNLENSLE SIEDNSCPGP MARQFSRDAS TSTVSIQGSG NHYHACAADD DFDTDFDPSI 

       790        800        810        820        830        840 
LPPPDPWIDS ITEDPLEAVQ RSVCHRDGHW FLKLLQAERD RMEGWCKLME REERENNLPE 

       850        860        870        880        890        900 
DILGKIRTAV GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS 

       910        920        930        940        950        960 
MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES SQRQEARKRL 

       970        980        990 
MAAKRAASVR QNSATESAES IEIYIPEAQT RL 

« Hide

Isoform 2 [UniParc].

Checksum: 9518AAB72BB13EE9
Show »

FASTA982109,390
Isoform 3 (GKAP1a) [UniParc].

Checksum: B45BBAFF2C4E8740
Show »

FASTA964107,331
Isoform 4 (GKAP1b) [UniParc].

Checksum: 224A365837BCE785
Show »

FASTA934104,064
Isoform 5 [UniParc].

Checksum: A562146ADAE2C929
Show »

FASTA53859,879
Isoform 6 [UniParc].

Checksum: 63EB6CC30E996F60
Show »

FASTA39744,247

References

« Hide 'large scale' references
[1]"Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse."
Welch J.M., Wang D., Feng G.
J. Comp. Neurol. 472:24-39(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
Strain: ICR.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 6), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 660-992 (ISOFORM 4).
Strain: C57BL/6J.
Tissue: Diencephalon and Testis.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Fetal brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 210-992 (ISOFORM 4).
Strain: C57BL/6.
Tissue: Brain.
[5]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362; SER-509; SER-516 AND SER-947, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[6]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
[7]"SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SHANK3, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY243846 mRNA. Translation: AAP70755.2.
AK031410 mRNA. Translation: BAC27391.1.
AK033901 mRNA. Translation: BAC28508.1.
AK034182 mRNA. Translation: BAC28619.1.
AK220515 mRNA. Translation: BAD90519.1. Different initiation.
BC062120 mRNA. Translation: AAH62120.1.
BC094369 mRNA. Translation: AAH94369.1.
CCDSCCDS28953.1. [Q9D415-1]
RefSeqNP_081988.3. NM_027712.3. [Q9D415-4]
NP_808307.2. NM_177639.6. [Q9D415-1]
XP_006524236.1. XM_006524173.1. [Q9D415-1]
XP_006524239.1. XM_006524176.1. [Q9D415-3]
UniGeneMm.311840.
Mm.367369.

3D structure databases

ProteinModelPortalQ9D415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230347. 2 interactions.
IntActQ9D415. 4 interactions.
MINTMINT-136157.

PTM databases

PhosphoSiteQ9D415.

Proteomic databases

MaxQBQ9D415.
PaxDbQ9D415.
PRIDEQ9D415.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000060072; ENSMUSP00000052858; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000133983; ENSMUSP00000116716; ENSMUSG00000003279. [Q9D415-2]
ENSMUST00000135938; ENSMUSP00000118497; ENSMUSG00000003279. [Q9D415-3]
ENSMUST00000155016; ENSMUSP00000122896; ENSMUSG00000003279. [Q9D415-1]
GeneID224997.
KEGGmmu:224997.
UCSCuc008dky.1. mouse. [Q9D415-6]
uc008dla.1. mouse. [Q9D415-5]
uc008dle.2. mouse. [Q9D415-1]
uc008dlf.2. mouse. [Q9D415-4]

Organism-specific databases

CTD9229.
MGIMGI:1346065. Dlgap1.
RougeSearch...

Phylogenomic databases

eggNOGNOG277836.
GeneTreeENSGT00550000074473.
HOVERGENHBG018957.
InParanoidQ9D415.
KOK15008.
OMAAVEVSSC.
OrthoDBEOG75QR37.
TreeFamTF321382.

Gene expression databases

BgeeQ9D415.
GenevestigatorQ9D415.

Family and domain databases

InterProIPR005026. GKAP.
[Graphical view]
PfamPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio377488.
PROQ9D415.
SOURCESearch...

Entry information

Entry nameDLGP1_MOUSE
AccessionPrimary (citable) accession number: Q9D415
Secondary accession number(s): Q52KF6 expand/collapse secondary AC list , Q5DTK5, Q6P6N4, Q6XBF4, Q8BZL7, Q8BZQ1, Q8C0G0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot