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Protein

Testis-specific serine/threonine-protein kinase 4

Gene

Tssk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May be involved in a signaling pathway during male germ cell development or mature sperm function. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-197, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei148Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 4 (EC:2.7.11.1)
Short name:
TSK-4
Short name:
TSSK-4
Short name:
Testis-specific kinase 4
Gene namesi
Name:Tssk4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1918349 Tssk4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867731 – 328Testis-specific serine/threonine-protein kinase 4Add BLAST328

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D411
PRIDEiQ9D411

PTM databases

iPTMnetiQ9D411
PhosphoSitePlusiQ9D411

Expressioni

Gene expression databases

CleanExiMM_TSSK4
ExpressionAtlasiQ9D411 baseline and differential

Interactioni

Protein-protein interaction databases

BioGridi214476, 1 interactor
STRINGi10090.ENSMUSP00000007735

Structurei

3D structure databases

ProteinModelPortaliQ9D411
SMRiQ9D411
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOVERGENiHBG101110
InParanoidiQ9D411
KOiK08811
PhylomeDBiQ9D411

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D411-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKGDTSETA SATPAYRSVM EEYGYEVGKI IGHGSYGTVY EAYYTKQKVM
60 70 80 90 100
VAVKIISKKK ASEDYLNKFL PREIQVMKVL RHKYLINFYQ AIETTSRVYI
110 120 130 140 150
ILELAQGGDV LEWIQRYGAC AETLAGKWFS QMALGIAYLH SKGIVHRDLK
160 170 180 190 200
LENLLLDKRE NVKISDFGFA KMVPSSQPVH SSPSYRQMNS LSHLSQTYCG
210 220 230 240 250
SFAYACPEIL LGLPYNPFLS DTWSMGVILY TLVVARLPFD DTNLKKLLRE
260 270 280 290 300
TQKEVTFPAN LTISQECKNL ILQLLRQSTK RATILDVLRD PWMLKFQPEQ
310 320
PSNEIRLLEA MYQPTSSAKR HQSLEITT
Length:328
Mass (Da):37,377
Last modified:June 1, 2001 - v1
Checksum:i64C66FAC6A1762B1
GO
Isoform 2 (identifier: Q9D411-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLTPSLSAAGR

Show »
Length:338
Mass (Da):38,331
Checksum:i705BD6C206D72F7B
GO

Sequence cautioni

The sequence BAB24429 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8E → G in BAB24429 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023561147R → RLTPSLSAAGR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461664 mRNA Translation: AAS17972.1
AK006144 mRNA Translation: BAB24429.1 Sequence problems.
AK016890 mRNA Translation: BAB30483.1
RefSeqiNP_001240818.1, NM_001253889.1
NP_081949.1, NM_027673.3 [Q9D411-1]
UniGeneiMm.171300

Genome annotation databases

EnsembliENSMUST00000226591; ENSMUSP00000153752; ENSMUSG00000007591 [Q9D411-1]
ENSMUST00000228395; ENSMUSP00000154783; ENSMUSG00000007591 [Q9D411-2]
GeneIDi71099
KEGGimmu:71099
UCSCiuc007tzy.2 mouse [Q9D411-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTSSK4_MOUSE
AccessioniPrimary (citable) accession number: Q9D411
Secondary accession number(s): Q9DA58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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