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Protein

Testis-specific serine/threonine-protein kinase 4

Gene

Tssk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in a signaling pathway during male germ cell development or mature sperm function. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-197, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei148Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 4 (EC:2.7.11.1)
Short name:
TSK-4
Short name:
TSSK-4
Short name:
Testis-specific kinase 4
Gene namesi
Name:Tssk4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1918349. Tssk4.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: UniProtKB
  • motile cilium Source: UniProtKB
  • nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867731 – 328Testis-specific serine/threonine-protein kinase 4Add BLAST328

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D411.
PRIDEiQ9D411.

PTM databases

iPTMnetiQ9D411.
PhosphoSitePlusiQ9D411.

Expressioni

Gene expression databases

CleanExiMM_TSSK4.

Interactioni

Protein-protein interaction databases

BioGridi214476. 1 interactor.
STRINGi10090.ENSMUSP00000007735.

Structurei

3D structure databases

ProteinModelPortaliQ9D411.
SMRiQ9D411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOVERGENiHBG101110.
InParanoidiQ9D411.
KOiK08811.
PhylomeDBiQ9D411.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D411-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKGDTSETA SATPAYRSVM EEYGYEVGKI IGHGSYGTVY EAYYTKQKVM
60 70 80 90 100
VAVKIISKKK ASEDYLNKFL PREIQVMKVL RHKYLINFYQ AIETTSRVYI
110 120 130 140 150
ILELAQGGDV LEWIQRYGAC AETLAGKWFS QMALGIAYLH SKGIVHRDLK
160 170 180 190 200
LENLLLDKRE NVKISDFGFA KMVPSSQPVH SSPSYRQMNS LSHLSQTYCG
210 220 230 240 250
SFAYACPEIL LGLPYNPFLS DTWSMGVILY TLVVARLPFD DTNLKKLLRE
260 270 280 290 300
TQKEVTFPAN LTISQECKNL ILQLLRQSTK RATILDVLRD PWMLKFQPEQ
310 320
PSNEIRLLEA MYQPTSSAKR HQSLEITT
Length:328
Mass (Da):37,377
Last modified:June 1, 2001 - v1
Checksum:i64C66FAC6A1762B1
GO
Isoform 2 (identifier: Q9D411-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLTPSLSAAGR

Show »
Length:338
Mass (Da):38,331
Checksum:i705BD6C206D72F7B
GO

Sequence cautioni

The sequence BAB24429 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8E → G in BAB24429 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023561147R → RLTPSLSAAGR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461664 mRNA. Translation: AAS17972.1.
AK006144 mRNA. Translation: BAB24429.1. Sequence problems.
AK016890 mRNA. Translation: BAB30483.1.
RefSeqiNP_001240818.1. NM_001253889.1.
NP_081949.1. NM_027673.3. [Q9D411-1]
UniGeneiMm.171300.

Genome annotation databases

GeneIDi71099.
KEGGimmu:71099.
UCSCiuc007tzy.2. mouse. [Q9D411-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461664 mRNA. Translation: AAS17972.1.
AK006144 mRNA. Translation: BAB24429.1. Sequence problems.
AK016890 mRNA. Translation: BAB30483.1.
RefSeqiNP_001240818.1. NM_001253889.1.
NP_081949.1. NM_027673.3. [Q9D411-1]
UniGeneiMm.171300.

3D structure databases

ProteinModelPortaliQ9D411.
SMRiQ9D411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214476. 1 interactor.
STRINGi10090.ENSMUSP00000007735.

PTM databases

iPTMnetiQ9D411.
PhosphoSitePlusiQ9D411.

Proteomic databases

PaxDbiQ9D411.
PRIDEiQ9D411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi71099.
KEGGimmu:71099.
UCSCiuc007tzy.2. mouse. [Q9D411-1]

Organism-specific databases

CTDi283629.
MGIiMGI:1918349. Tssk4.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOVERGENiHBG101110.
InParanoidiQ9D411.
KOiK08811.
PhylomeDBiQ9D411.

Miscellaneous databases

PROiQ9D411.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TSSK4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK4_MOUSE
AccessioniPrimary (citable) accession number: Q9D411
Secondary accession number(s): Q9DA58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.