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Protein

Centrosomal protein of 72 kDa

Gene

Cep72

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs) (By similarity). Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 72 kDa
Short name:
Cep72
Gene namesi
Name:Cep72
Synonyms:Kiaa1519
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1921720. Cep72.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriolar satellite By similarity

  • Note: Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule-dependent manner (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000895001 – 646Centrosomal protein of 72 kDaAdd BLAST646

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphoserineCombined sources1
Modified residuei236PhosphoserineBy similarity1
Modified residuei380PhosphoserineBy similarity1
Modified residuei402PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D3R3.
PeptideAtlasiQ9D3R3.
PRIDEiQ9D3R3.

PTM databases

iPTMnetiQ9D3R3.
PhosphoSitePlusiQ9D3R3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021572.
CleanExiMM_CEP72.
GenevisibleiQ9D3R3. MM.

Interactioni

Subunit structurei

Interacts with KIZ, PCM1 and CDK5RAP2.By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI

Protein-protein interaction databases

BioGridi216776. 53 interactors.
IntActiQ9D3R3. 53 interactors.
MINTiMINT-218886.
STRINGi10090.ENSMUSP00000037788.

Structurei

3D structure databases

ProteinModelPortaliQ9D3R3.
SMRiQ9D3R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 49LRR 1Add BLAST22
Repeati54 – 75LRR 2Add BLAST22
Repeati76 – 97LRR 3Add BLAST22
Domaini110 – 149LRRCTAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili476 – 622Sequence analysisAdd BLAST147

Sequence similaritiesi

Belongs to the CEP72 family.Curated
Contains 3 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IH75. Eukaryota.
ENOG4111P3A. LUCA.
GeneTreeiENSGT00530000063884.
HOVERGENiHBG050900.
InParanoidiQ9D3R3.
KOiK16532.
OMAiQHLDKSL.
OrthoDBiEOG091G04WS.
TreeFamiTF338646.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00369. LRR_TYP. 2 hits.
SM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D3R3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPGQRLVLC EETVRERSGL GPHRDLAELR SLSIPGTYQE KITHLGNSLM
60 70 80 90 100
HLTALKSLDL SRNSLVSLEG IQYLVSLESL NLYYNCISSL AEVFRLHTLL
110 120 130 140 150
ELQDVDFRLN PVVKNESDYR LFVVHMLPKL RQLDDRPVRE SERKASQLHF
160 170 180 190 200
APEDSLNSKE NFSTTLTVGR PHHLRNRCTE TSAKKCLVMD ADDEAVLNLI
210 220 230 240 250
AECEWDLSNP PGNMSSSQKE HEADLHYAQE SRHLLSPLSI QHQCGDSARR
260 270 280 290 300
GHEKKKVTSR GCPGHSPQDQ LCGELPLQHG LPEACHMHVQ HARITSQPDS
310 320 330 340 350
VDVEDCASSA QKSSLSSQKT VNPLPVPEKY RKRRMPGGRF QVPSDRECLS
360 370 380 390 400
FLERADGPSS LEDSLSRQDG LEGQSQVALS HSEALEAEER TSHGTSDPRV
410 420 430 440 450
LSPKLCSAAV PERRSTLEVA LLEALLDLID RCSSGSGSLH GNEAFLAQAK
460 470 480 490 500
HVLSSLQEFT ATRDSSALEK EGIGYLSLEN KTLQSRLAEQ QQQYTATVTK
510 520 530 540 550
MTAELNNTKR ELDTLRQHLD KSLEENSHLK SLLYNVKKEV KTADTSTALT
560 570 580 590 600
LQITGLQASM KQLSGEVVEL KQHVEHYDKI QELTQMLQES HSSLVSTNEH
610 620 630 640
LLQELSRTRA QHRAEVEQMR WSFQEFKKTT ALIPHRSSRR GGRQSC
Length:646
Mass (Da):72,433
Last modified:July 27, 2011 - v3
Checksum:i9E2A803B0E0883AC
GO
Isoform 2 (identifier: Q9D3R3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-286: Missing.
     321-400: Missing.

Show »
Length:280
Mass (Da):31,339
Checksum:iA0285EE855EB5826
GO

Sequence cautioni

The sequence BAC28870 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD32467 differs from that shown. The sequence differs from that shown because it seems to be derived from a pre-mRNA.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18 – 19SG → RA in BAB30613 (PubMed:16141072).Curated2
Sequence conflicti301V → L in BAB27729 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0127481 – 286Missing in isoform 2. 1 PublicationAdd BLAST286
Alternative sequenceiVSP_012749321 – 400Missing in isoform 2. 1 PublicationAdd BLAST80

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011607 mRNA. Translation: BAB27729.1.
AK017134 mRNA. Translation: BAB30613.2.
AK034892 mRNA. Translation: BAC28870.1. Different initiation.
AK161033 mRNA. Translation: BAE36159.1.
CT010471 Genomic DNA. No translation available.
BC100549 mRNA. Translation: AAI00550.1.
AK173189 Transcribed RNA. Translation: BAD32467.1. Sequence problems.
CCDSiCCDS26639.1. [Q9D3R3-1]
RefSeqiNP_083235.3. NM_028959.3. [Q9D3R3-1]
UniGeneiMm.73234.

Genome annotation databases

EnsembliENSMUST00000036456; ENSMUSP00000037788; ENSMUSG00000021572. [Q9D3R3-1]
GeneIDi74470.
KEGGimmu:74470.
UCSCiuc007rep.2. mouse. [Q9D3R3-2]
uc007req.2. mouse. [Q9D3R3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011607 mRNA. Translation: BAB27729.1.
AK017134 mRNA. Translation: BAB30613.2.
AK034892 mRNA. Translation: BAC28870.1. Different initiation.
AK161033 mRNA. Translation: BAE36159.1.
CT010471 Genomic DNA. No translation available.
BC100549 mRNA. Translation: AAI00550.1.
AK173189 Transcribed RNA. Translation: BAD32467.1. Sequence problems.
CCDSiCCDS26639.1. [Q9D3R3-1]
RefSeqiNP_083235.3. NM_028959.3. [Q9D3R3-1]
UniGeneiMm.73234.

3D structure databases

ProteinModelPortaliQ9D3R3.
SMRiQ9D3R3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216776. 53 interactors.
IntActiQ9D3R3. 53 interactors.
MINTiMINT-218886.
STRINGi10090.ENSMUSP00000037788.

PTM databases

iPTMnetiQ9D3R3.
PhosphoSitePlusiQ9D3R3.

Proteomic databases

PaxDbiQ9D3R3.
PeptideAtlasiQ9D3R3.
PRIDEiQ9D3R3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036456; ENSMUSP00000037788; ENSMUSG00000021572. [Q9D3R3-1]
GeneIDi74470.
KEGGimmu:74470.
UCSCiuc007rep.2. mouse. [Q9D3R3-2]
uc007req.2. mouse. [Q9D3R3-1]

Organism-specific databases

CTDi55722.
MGIiMGI:1921720. Cep72.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IH75. Eukaryota.
ENOG4111P3A. LUCA.
GeneTreeiENSGT00530000063884.
HOVERGENiHBG050900.
InParanoidiQ9D3R3.
KOiK16532.
OMAiQHLDKSL.
OrthoDBiEOG091G04WS.
TreeFamiTF338646.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ9D3R3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021572.
CleanExiMM_CEP72.
GenevisibleiQ9D3R3. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00369. LRR_TYP. 2 hits.
SM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEP72_MOUSE
AccessioniPrimary (citable) accession number: Q9D3R3
Secondary accession number(s): E9QQ95
, Q3TU12, Q497H8, Q69ZH7, Q8BM53, Q9D0B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.