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Q9D3G9

- RHOH_MOUSE

UniProt

Q9D3G9 - RHOH_MOUSE

Protein

Rho-related GTP-binding protein RhoH

Gene

Rhoh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils By similarity. Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca2+ mobilization in mast cells.By similarity4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188GTPBy similarity
    Nucleotide bindingi58 – 625GTPBy similarity
    Nucleotide bindingi116 – 1194GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. kinase inhibitor activity Source: Ensembl
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. mast cell activation Source: UniProtKB
    2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    3. small GTPase mediated signal transduction Source: InterPro
    4. T cell differentiation Source: UniProtKB

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho-related GTP-binding protein RhoH
    Gene namesi
    Name:Rhoh
    Synonyms:Arhh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1921984. Rhoh.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
    Note: Colocalizes together with ZAP70 in the immunological synapse.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. immunological synapse Source: UniProtKB
    3. mitochondrion Source: Ensembl
    4. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice show impaired passive systemic anaphylaxis and histamine release upon challenge with the specific antigen and impaired T-cell differentiation.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi73 – 731Y → F: Abolishes interaction with ZAP70; when associated with F-83. 1 Publication
    Mutagenesisi83 – 831Y → F: Abolishes interaction with ZAP70; when associated with F-73. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Rho-related GTP-binding protein RhoHPRO_0000198868Add
    BLAST
    Propeptidei189 – 1913Removed in mature formBy similarityPRO_0000281219

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881Cysteine methyl esterBy similarity
    Lipidationi188 – 1881S-geranylgeranyl cysteineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development.1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PRIDEiQ9D3G9.

    Expressioni

    Tissue specificityi

    Expression is widespread in hematopoietic cells, including in bone marrow progenitor cells and in differentiated myeloid as well as lymphoid cells. Expressed at high levels in the thymus and mast cells, found in spleen and low-density bone marrow (LDBM) cells and is detected at a low level in neutrophils. In the thymus it is detected in thymocytes of the thymic cortex but not in non-lymphoid cells of fibrovascular and fibroadipose tissues. Expressed in T-cells, B-cells and mast cells.2 Publications

    Developmental stagei

    Expressed at all stages of thymocyte development, with relative peaks at the DN3 and DP stages.1 Publication

    Gene expression databases

    ArrayExpressiQ9D3G9.
    BgeeiQ9D3G9.
    CleanExiMM_RHOH.
    GenevestigatoriQ9D3G9.

    Interactioni

    Subunit structurei

    Interacts with GDI1 and GDI2 By similarity. Interacts with ZAP70 (via SH2 domains) and the interaction is enhanced by its phosphorylation by LCK. Interacts with SYK and the interaction is enhanced by its phosphorylation by FYN.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi216981. 1 interaction.
    STRINGi10090.ENSMUSP00000031106.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D3G9.
    SMRiQ9D3G9. Positions 4-168.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni73 – 8614Interaction with ZAP70Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi33 – 419Effector regionBy similarity

    Domaini

    The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM).

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00720000108472.
    HOGENOMiHOG000233974.
    HOVERGENiHBG009351.
    InParanoidiQ9D3G9.
    KOiK07873.
    OMAiASCINAI.
    OrthoDBiEOG7TJ3K0.
    PhylomeDBiQ9D3G9.
    TreeFamiTF331219.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D3G9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSSIKCVLV GDSAVGKTSL LVRFTSETFP EAYKPTVYEN TGVDVFMDGI    50
    QISLGLWDTA GNDAFRSIRP LSYQQADVVL MCYSVANHNS FLNLKNKWIS 100
    EIRSNLPCTP VLVVATQTDQ REVGPHRASC INAIEGKRLA QDVRAKGYLE 150
    CSALSNRGVQ QVFECAVRTA VNQARRRNRR KLFSINECKI F 191
    Length:191
    Mass (Da):21,324
    Last modified:June 1, 2001 - v1
    Checksum:i18845201F647D539
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK017885 mRNA. Translation: BAB30987.1.
    CCDSiCCDS39100.1.
    RefSeqiNP_001074574.1. NM_001081105.1.
    UniGeneiMm.20323.

    Genome annotation databases

    EnsembliENSMUST00000031106; ENSMUSP00000031106; ENSMUSG00000029204.
    GeneIDi74734.
    KEGGimmu:74734.
    UCSCiuc008xoc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK017885 mRNA. Translation: BAB30987.1 .
    CCDSi CCDS39100.1.
    RefSeqi NP_001074574.1. NM_001081105.1.
    UniGenei Mm.20323.

    3D structure databases

    ProteinModelPortali Q9D3G9.
    SMRi Q9D3G9. Positions 4-168.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216981. 1 interaction.
    STRINGi 10090.ENSMUSP00000031106.

    Proteomic databases

    PRIDEi Q9D3G9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031106 ; ENSMUSP00000031106 ; ENSMUSG00000029204 .
    GeneIDi 74734.
    KEGGi mmu:74734.
    UCSCi uc008xoc.1. mouse.

    Organism-specific databases

    CTDi 399.
    MGIi MGI:1921984. Rhoh.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00720000108472.
    HOGENOMi HOG000233974.
    HOVERGENi HBG009351.
    InParanoidi Q9D3G9.
    KOi K07873.
    OMAi ASCINAI.
    OrthoDBi EOG7TJ3K0.
    PhylomeDBi Q9D3G9.
    TreeFami TF331219.

    Enzyme and pathway databases

    Reactomei REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    NextBioi 341522.
    PROi Q9D3G9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D3G9.
    Bgeei Q9D3G9.
    CleanExi MM_RHOH.
    Genevestigatori Q9D3G9.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RhoH, a hematopoietic-specific Rho GTPase, regulates proliferation, survival, migration, and engraftment of hematopoietic progenitor cells."
      Gu Y., Jasti A.C., Jansen M., Siefring J.E.
      Blood 105:1467-1475(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    3. "RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development."
      Gu Y., Chae H.-D., Siefring J.E., Jasti A.C., Hildeman D.A., Williams D.A.
      Nat. Immunol. 7:1182-1190(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ZAP70, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF TYR-73 AND TYR-83.
    4. Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    5. "RhoH plays critical roles in Fc epsilon RI-dependent signal transduction in mast cells."
      Oda H., Fujimoto M., Patrick M.S., Chida D., Sato Y., Azuma Y., Aoki H., Abe T., Suzuki H., Shirai M.
      J. Immunol. 182:957-962(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH SYK, TISSUE SPECIFICITY.
    6. Cited for: INDUCTION.

    Entry informationi

    Entry nameiRHOH_MOUSE
    AccessioniPrimary (citable) accession number: Q9D3G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3