Q9D3G9 (RHOH_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 98. History...
Names and origin
|Protein names||Recommended name:|
Rho-related GTP-binding protein RhoH
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||191 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils By similarity. Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca2+ mobilization in mast cells. Ref.1 Ref.3 Ref.4 Ref.5
Interacts with GDI1 and GDI2 By similarity. Interacts with ZAP70 (via SH2 domains) and the interaction is enhanced by its phosphorylation by LCK. Interacts with SYK and the interaction is enhanced by its phosphorylation by FYN. Ref.3 Ref.5
Expression is widespread in hematopoietic cells, including in bone marrow progenitor cells and in differentiated myeloid as well as lymphoid cells. Expressed at high levels in the thymus and mast cells, found in spleen and low-density bone marrow (LDBM) cells and is detected at a low level in neutrophils. In the thymus it is detected in thymocytes of the thymic cortex but not in non-lymphoid cells of fibrovascular and fibroadipose tissues. Expressed in T-cells, B-cells and mast cells. Ref.1 Ref.5
Expressed at all stages of thymocyte development, with relative peaks at the DN3 and DP stages. Ref.4
The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM).
Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development. Ref.3
|Cellular component||Cell membrane|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||T cell differentiationmast cell activationnegative regulation of I-kappaB kinase/NF-kappaB signaling|
Inferred from electronic annotation. Source: Ensemblsmall GTPase mediated signal transduction
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: Ensemblplasma membrane
Inferred from electronic annotation. Source: UniProtKB-KWkinase inhibitor activity
Inferred from electronic annotation. Source: Ensemblprotein binding
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 188||188||Rho-related GTP-binding protein RhoH||PRO_0000198868|
|Propeptide||189 – 191||3||Removed in mature form By similarity||PRO_0000281219|
|Nucleotide binding||11 – 18||8||GTP By similarity|
|Nucleotide binding||58 – 62||5||GTP By similarity|
|Nucleotide binding||116 – 119||4||GTP By similarity|
|Region||73 – 86||14||Interaction with ZAP70|
|Motif||33 – 41||9||Effector region By similarity|
Amino acid modifications
|Modified residue||188||1||Cysteine methyl ester By similarity|
|Lipidation||188||1||S-geranylgeranyl cysteine By similarity|
|Mutagenesis||73||1||Y → F: Abolishes interaction with ZAP70; when associated with F-83. Ref.3|
|Mutagenesis||83||1||Y → F: Abolishes interaction with ZAP70; when associated with F-73. Ref.3|
|||"RhoH, a hematopoietic-specific Rho GTPase, regulates proliferation, survival, migration, and engraftment of hematopoietic progenitor cells."|
Gu Y., Jasti A.C., Jansen M., Siefring J.E.
Blood 105:1467-1475(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
|||"The transcriptional landscape of the mammalian genome."|
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|||"RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development."|
Gu Y., Chae H.-D., Siefring J.E., Jasti A.C., Hildeman D.A., Williams D.A.
Nat. Immunol. 7:1182-1190(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ZAP70, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF TYR-73 AND TYR-83.
|||"RhoH is important for positive thymocyte selection and T-cell receptor signaling."|
Dorn T., Kuhn U., Bungartz G., Stiller S., Bauer M., Ellwart J., Peters T., Scharffetter-Kochanek K., Semmrich M., Laschinger M., Holzmann B., Klinkert W.E.F., Straten P.T., Kollgaard T., Sixt M., Brakebusch C.
Blood 109:2346-2355(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
|||"RhoH plays critical roles in Fc epsilon RI-dependent signal transduction in mast cells."|
Oda H., Fujimoto M., Patrick M.S., Chida D., Sato Y., Azuma Y., Aoki H., Abe T., Suzuki H., Shirai M.
J. Immunol. 182:957-962(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH SYK, TISSUE SPECIFICITY.
|||"RhoH/TTF negatively regulates leukotriene production in neutrophils."|
Daryadel A., Yousefi S., Troi D., Schmid I., Schmidt-Mende J., Mordasini C., Dahinden C.A., Ziemiecki A., Simon H.-U.
J. Immunol. 182:6527-6532(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
|+||Additional computationally mapped references.|
|AK017885 mRNA. Translation: BAB30987.1.|
|RefSeq||NP_001074574.1. NM_001081105.1. |
3D structure databases
|SMR||Q9D3G9. Positions 4-168. |
Protein-protein interaction databases
|BioGrid||216981. 1 interaction.|
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSMUST00000031106; ENSMUSP00000031106; ENSMUSG00000029204. |
|UCSC||uc008xoc.1. mouse. |
|MGI||MGI:1921984. Rhoh. |
Gene expression databases
Family and domain databases
|Gene3D||188.8.131.520. 1 hit. |
|InterPro||IPR027417. P-loop_NTPase. |
|Pfam||PF00071. Ras. 1 hit. |
|PRINTS||PR00449. RASTRNSFRMNG. |
|SMART||SM00174. RHO. 1 hit. |
|SUPFAM||SSF52540. SSF52540. 1 hit. |
|TIGRFAMs||TIGR00231. small_GTP. 1 hit. |
|PROSITE||PS51420. RHO. 1 hit. |
|Accession||Primary (citable) accession number: Q9D3G9|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|