Q9D3A8 (CAPON_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein

Alternative name(s):
C-terminal PDZ ligand of neuronal nitric oxide synthase protein
Nitric oxide synthase 1 adaptor protein

Gene names

Name:	Nos1ap
Synonyms:	Capon, Kiaa0464

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 By similarity.
Subunit structure	Interacts with the PDZ domain of NOS1 or the second PDZ domain of DLG4 through its C-terminus. Interacts with RASD1 and SYN1, SYN2 and SYN3 via its PID domain. Forms a ternary complex with NOS1 and SYN1 By similarity. Forms a ternary complex with NOS1 and RASD1. Ref.4
Sequence similarities	Contains 1 PID domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D3A8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q9D3A8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     313-325: LKDQLAAEAAARL → SLLPSSQAPLSWA
     326-503: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein		PRO_0000089317
Regions
	Domain	26 – 191	166	PID
	Region	491 – 503	13	Interaction with NOS1 By similarity
	Coiled coil	318 – 359	42	Potential
	Motif	501 – 503	3	PDZ-binding By similarity
	Compositional bias	269 – 272	4	Poly-Ser
	Compositional bias	297 – 304	8	Poly-Gln
Amino acid modifications
	Modified residue	183	1	Phosphoserine Ref.5
	Modified residue	187	1	Phosphoserine Ref.5
	Modified residue	262	1	Phosphoserine By similarity
	Modified residue	370	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	313 – 325	13	LKDQL…AAARL → SLLPSSQAPLSWA in isoform 2.		VSP_012462
	Alternative sequence	326 – 503	178	Missing in isoform 2.		VSP_012463
Experimental info
	Sequence conflict	25	1	A → S in BAB31095. Ref.1
	Sequence conflict	110	1	R → K in BAB31095. Ref.1
	Sequence conflict	157	1	G → A in BAB31095. Ref.1
	Sequence conflict	311	1	H → L in BAB31095. Ref.1
	Sequence conflict	311	1	H → L in BAC65572. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 1BD365C67437C8A5	FASTA	503	55,873
	10 20 30 40 50 60 MPSKTKYNLV DDGHDLRIPL HNEDAFQHGI SFEAKYVGSL DVPRPNSRVE IVAAMRRIRY 70 80 90 100 110 120 EFKAKNIKKK KVSIMVSVDG VKVILKKKKK KKEWTWDESK MLVMQDPIYR IFYVSHDSQD 130 140 150 160 170 180 LKIFSYIARD GASNIFRCNV FKSKKKSQAM RIVRTVGQAF EVCHKLSLQH TQQNADGQED 190 200 210 220 230 240 GESERNSDGS GDPGRQLTGA ERVSTAAAEE TDIDAVEVPL PGNDILEFSR GVTDLDAVGK 250 260 270 280 290 300 DGGSHIDSTV SPHPQEPMLT ASPRMLLPSS SSKPPGLGTG TPLSTHHQMQ LLQQLLQQQQ 310 320 330 340 350 360 QQTQVAVAQV HLLKDQLAAE AAARLEAQAR VHQLLLQNKD MLQHISLLVK QVQELELKLS 370 380 390 400 410 420 GQNTMGSQDS LLEITFRSGA LPVLCESTTP KPEDLHSPLL GAGLADFAHP AGSPLGRHDC 430 440 450 460 470 480 LVKLECFRFL PPEDTQPMMA QGEPLLGGLE LIKFRESGIA SEYESNTDES EERDSWSQEE 490 500 LPRLLNVLQR QELGDSLDDE IAV
	Isoform 2 [UniParc]. Checksum: 1A7EE296CA7A9D87	FASTA	325	36,122
	10 20 30 40 50 60 MPSKTKYNLV DDGHDLRIPL HNEDAFQHGI SFEAKYVGSL DVPRPNSRVE IVAAMRRIRY 70 80 90 100 110 120 EFKAKNIKKK KVSIMVSVDG VKVILKKKKK KKEWTWDESK MLVMQDPIYR IFYVSHDSQD 130 140 150 160 170 180 LKIFSYIARD GASNIFRCNV FKSKKKSQAM RIVRTVGQAF EVCHKLSLQH TQQNADGQED 190 200 210 220 230 240 GESERNSDGS GDPGRQLTGA ERVSTAAAEE TDIDAVEVPL PGNDILEFSR GVTDLDAVGK 250 260 270 280 290 300 DGGSHIDSTV SPHPQEPMLT ASPRMLLPSS SSKPPGLGTG TPLSTHHQMQ LLQQLLQQQQ 310 320 QQTQVAVAQV HLSLLPSSQA PLSWA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Medulla oblongata.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-503 (ISOFORM 1). Tissue: Brain.
[4]	"Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON." Fang M., Jaffrey S.R., Sawa A., Ye K., Luo X., Snyder S.H. Neuron 28:183-193(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RASD1 AND NOS1.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-187 AND SER-370, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK018149 mRNA. Translation: BAB31095.1. AC119431 Genomic DNA. No translation available. AC123650 Genomic DNA. No translation available. AC125017 Genomic DNA. No translation available. AC125021 Genomic DNA. No translation available. AK122290 Transcribed RNA. Translation: BAC65572.3.
IPI	IPI00135786. IPI00515529.
RefSeq	NP_001103455.1. NM_001109985.1.
UniGene	Mm.487078.
3D structure databases
ProteinModelPortal	Q9D3A8.
SMR	Q9D3A8. Positions 29-162.
ModBase	Search...
PTM databases
PhosphoSite	Q9D3A8.
Proteomic databases
PRIDE	Q9D3A8.
Protocols and materials databases
DNASU	70729.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000160456; ENSMUSP00000125251; ENSMUSG00000038473.
GeneID	70729.
KEGG	mmu:70729.
UCSC	uc011wvt.1. mouse.
Organism-specific databases
CTD	9722.
MGI	MGI:1917979. Nos1ap.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG244289.
GeneTree	ENSGT00510000046975.
HOGENOM	HOG000111298.
HOVERGEN	HBG050788.
InParanoid	Q9D3A8.
KO	K16513.
OMA	QNTMGSQ.
OrthoDB	EOG49KFQP.
Gene expression databases
ArrayExpress	Q9D3A8.
Bgee	Q9D3A8.
Genevestigator	Q9D3A8.
GermOnline	ENSMUSG00000038473. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR006020. PTyr_interaction_dom. [Graphical view]
Pfam	PF00640. PID. 1 hit. [Graphical view]
SMART	SM00462. PTB. 1 hit. [Graphical view]
PROSITE	PS01179. PID. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	332169.
SOURCE	Search...

Entry information

Entry name

CAPON_MOUSE

Accession

Primary (citable) accession number: Q9D3A8
Secondary accession number(s): E9Q7B9, Q80TZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program