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Q9D3A8 (CAPON_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
Alternative name(s):
C-terminal PDZ ligand of neuronal nitric oxide synthase protein
Nitric oxide synthase 1 adaptor protein
Gene names
Name:Nos1ap
Synonyms:Capon, Kiaa0464
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 By similarity.

Subunit structure

Interacts with the PDZ domain of NOS1 or the second PDZ domain of DLG4 through its C-terminus. Interacts with RASD1 and SYN1, SYN2 and SYN3 via its PID domain. Forms a ternary complex with NOS1 and SYN1 By similarity. Forms a ternary complex with NOS1 and RASD1. Ref.4

Sequence similarities

Contains 1 PID domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9D3A8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q9D3A8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     313-325: LKDQLAAEAAARL → SLLPSSQAPLSWA
     326-503: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
PRO_0000089317

Regions

Domain26 – 191166PID
Region491 – 50313Interaction with NOS1 By similarity
Coiled coil318 – 35942 Potential
Motif501 – 5033PDZ-binding By similarity
Compositional bias269 – 2724Poly-Ser
Compositional bias297 – 3048Poly-Gln

Amino acid modifications

Modified residue1831Phosphoserine Ref.5
Modified residue1871Phosphoserine Ref.5
Modified residue1901Phosphoserine Ref.5
Modified residue2621Phosphoserine By similarity
Modified residue3701Phosphoserine Ref.5

Natural variations

Alternative sequence313 – 32513LKDQL…AAARL → SLLPSSQAPLSWA in isoform 2.
VSP_012462
Alternative sequence326 – 503178Missing in isoform 2.
VSP_012463

Experimental info

Sequence conflict251A → S in BAB31095. Ref.1
Sequence conflict1101R → K in BAB31095. Ref.1
Sequence conflict1571G → A in BAB31095. Ref.1
Sequence conflict3111H → L in BAB31095. Ref.1
Sequence conflict3111H → L in BAC65572. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 1BD365C67437C8A5

FASTA50355,873
        10         20         30         40         50         60 
MPSKTKYNLV DDGHDLRIPL HNEDAFQHGI SFEAKYVGSL DVPRPNSRVE IVAAMRRIRY 

        70         80         90        100        110        120 
EFKAKNIKKK KVSIMVSVDG VKVILKKKKK KKEWTWDESK MLVMQDPIYR IFYVSHDSQD 

       130        140        150        160        170        180 
LKIFSYIARD GASNIFRCNV FKSKKKSQAM RIVRTVGQAF EVCHKLSLQH TQQNADGQED 

       190        200        210        220        230        240 
GESERNSDGS GDPGRQLTGA ERVSTAAAEE TDIDAVEVPL PGNDILEFSR GVTDLDAVGK 

       250        260        270        280        290        300 
DGGSHIDSTV SPHPQEPMLT ASPRMLLPSS SSKPPGLGTG TPLSTHHQMQ LLQQLLQQQQ 

       310        320        330        340        350        360 
QQTQVAVAQV HLLKDQLAAE AAARLEAQAR VHQLLLQNKD MLQHISLLVK QVQELELKLS 

       370        380        390        400        410        420 
GQNTMGSQDS LLEITFRSGA LPVLCESTTP KPEDLHSPLL GAGLADFAHP AGSPLGRHDC 

       430        440        450        460        470        480 
LVKLECFRFL PPEDTQPMMA QGEPLLGGLE LIKFRESGIA SEYESNTDES EERDSWSQEE 

       490        500 
LPRLLNVLQR QELGDSLDDE IAV 

« Hide

Isoform 2 [UniParc].

Checksum: 1A7EE296CA7A9D87
Show »

FASTA32536,122

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Medulla oblongata.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-503 (ISOFORM 1).
Tissue: Brain.
[4]"Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON."
Fang M., Jaffrey S.R., Sawa A., Ye K., Luo X., Snyder S.H.
Neuron 28:183-193(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RASD1 AND NOS1.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-187; SER-190 AND SER-370, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK018149 mRNA. Translation: BAB31095.1.
AC119431 Genomic DNA. No translation available.
AC123650 Genomic DNA. No translation available.
AC125017 Genomic DNA. No translation available.
AC125021 Genomic DNA. No translation available.
AK122290 Transcribed RNA. Translation: BAC65572.3.
RefSeqNP_001103455.1. NM_001109985.1.
UniGeneMm.487078.

3D structure databases

ProteinModelPortalQ9D3A8.
SMRQ9D3A8. Positions 29-162.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9D3A8.

Proteomic databases

PRIDEQ9D3A8.

Protocols and materials databases

DNASU70729.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000160456; ENSMUSP00000125251; ENSMUSG00000038473. [Q9D3A8-1]
GeneID70729.
KEGGmmu:70729.
UCSCuc011wvt.1. mouse. [Q9D3A8-1]

Organism-specific databases

CTD9722.
MGIMGI:1917979. Nos1ap.
RougeSearch...

Phylogenomic databases

eggNOGNOG244289.
GeneTreeENSGT00510000046975.
HOGENOMHOG000111298.
HOVERGENHBG050788.
InParanoidQ9D3A8.
KOK16513.
OMAQNTMGSQ.
OrthoDBEOG7JDQXM.
TreeFamTF317226.

Gene expression databases

ArrayExpressQ9D3A8.
BgeeQ9D3A8.
GenevestigatorQ9D3A8.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamPF00640. PID. 1 hit.
[Graphical view]
SMARTSM00462. PTB. 1 hit.
[Graphical view]
PROSITEPS01179. PID. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio332169.
PROQ9D3A8.
SOURCESearch...

Entry information

Entry nameCAPON_MOUSE
AccessionPrimary (citable) accession number: Q9D3A8
Secondary accession number(s): E9Q7B9, Q80TZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot