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Protein

Epoxide hydrolase 1

Gene

Ephx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids.By similarity

Catalytic activityi

Cis-stilbene oxide + H2O = (1R,2R)-1,2-diphenylethane-1,2-diol.By similarity
1-(4-methoxyphenyl)-N-methyl-N-((3-methyloxetan-3-yl)methyl)methanamine + H2O = 2-((((4-methoxyphenyl)methyl)(methyl)amino)methyl)-2-methylpropane-1,3-diol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226NucleophileBy similarity1
Active sitei374Proton donorBy similarity1
Active sitei431Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAromatic hydrocarbons catabolism, Detoxification

Enzyme and pathway databases

ReactomeiR-MMU-211945 Phase I - Functionalization of compounds
SABIO-RKiQ9D379

Protein family/group databases

ESTHERimouse-EPHX1 Epoxide_hydrolase
MEROPSiS33.971

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxide hydrolase 1 (EC:3.3.2.9By similarity)
Alternative name(s):
Epoxide hydratase
Microsomal epoxide hydrolase
Short name:
mEHCurated
Gene namesi
Name:Ephx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:95405 Ephx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini22 – 455CytoplasmicBy similarityAdd BLAST434

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075293

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000808561 – 455Epoxide hydrolase 1Add BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295Dimethylated arginine; alternateBy similarity1
Modified residuei295Omega-N-methylated arginine; alternateBy similarity1
Modified residuei439N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

MaxQBiQ9D379
PaxDbiQ9D379
PeptideAtlasiQ9D379
PRIDEiQ9D379

PTM databases

iPTMnetiQ9D379
PhosphoSitePlusiQ9D379
SwissPalmiQ9D379

Expressioni

Gene expression databases

BgeeiENSMUSG00000038776
CleanExiMM_EPHX1
ExpressionAtlasiQ9D379 baseline and differential
GenevisibleiQ9D379 MM

Interactioni

Protein-protein interaction databases

IntActiQ9D379, 6 interactors
MINTiQ9D379
STRINGi10090.ENSMUSP00000047551

Chemistry databases

BindingDBiQ9D379

Structurei

3D structure databases

ProteinModelPortaliQ9D379
SMRiQ9D379
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2565 Eukaryota
COG0596 LUCA
GeneTreeiENSGT00390000002210
HOGENOMiHOG000132894
HOVERGENiHBG002366
InParanoidiQ9D379
KOiK01253
OMAiFSEAPHQ
OrthoDBiEOG091G0B5A
TreeFamiTF313813

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000639 Epox_hydrolase-like
IPR010497 Epoxide_hydro_N
IPR016292 Epoxide_hydrolase
PfamiView protein in Pfam
PF06441 EHN, 1 hit
PIRSFiPIRSF001112 Epoxide_hydrolase, 1 hit
PRINTSiPR00412 EPOXHYDRLASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLELILASV LGFVIYWFVS RDKEETLPLE DGWWGPGSKP SAKEDESIRP
60 70 80 90 100
FKVETSDEEI KDLHQRIDRF RASPPLEGSR FHYGFNSSYL KKVVSFWRNE
110 120 130 140 150
FDWRKQVEIL NQYPHFKTKI EGLDIHFIHV KPPQLPSGRT PKPLLMVHGW
160 170 180 190 200
PGSFYEFYKI IPLLTDPKTH GLSDEHVFEV ICPSIPGYGF SEASSKKGLN
210 220 230 240 250
SVATARIFYK LMSRLGFQKF YIQGGDWGSL ICTNIAQMVP NHVKGLHLNM
260 270 280 290 300
SFISRNIYSL TPLLGQRFGR FLGYTEKDLE LLYPFKEKVF YNIMRESGYL
310 320 330 340 350
HIQATKPDTV GCALNDSPVG LAAYILEKFS TWTKSEYREL EDGGLERKFS
360 370 380 390 400
LEDLLTNIMI YWTTGTIVSS QRFYKENLGQ GVMVHRHEGM KVFVPTGYSA
410 420 430 440 450
FPSEILHAPE KWVKVKYPKL ISYSYMERGG HFAAFEEPKL LAQDIRKFVS

LAELQ
Length:455
Mass (Da):52,577
Last modified:July 27, 2011 - v2
Checksum:i1567F619D9114C58
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti410E → K in BAB31132 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89491 mRNA Translation: AAB49762.1
AK018249 mRNA Translation: BAB31132.1
CH466555 Genomic DNA Translation: EDL13136.1
BC045194 mRNA Translation: AAH45194.1
BC061493 mRNA Translation: AAH61493.1
CCDSiCCDS15579.1
RefSeqiNP_001299847.1, NM_001312918.1
NP_034275.1, NM_010145.3
UniGeneiMm.9075

Genome annotation databases

EnsembliENSMUST00000036928; ENSMUSP00000047551; ENSMUSG00000038776
GeneIDi13849
KEGGimmu:13849
UCSCiuc007dwz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiHYEP_MOUSE
AccessioniPrimary (citable) accession number: Q9D379
Secondary accession number(s): P97869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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