Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Keratin, type I cytoskeletal 20

Gene

Krt20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.2 Publications

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Curated

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cellular response to starvation Source: UniProtKB
  • intermediate filament organization Source: UniProtKB
  • regulation of protein secretion Source: UniProtKB

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-MMU-6805567 Keratinization
R-MMU-6809371 Formation of the cornified envelope

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 20
Alternative name(s):
Cytokeratin-20
Short name:
CK-20
Keratin-20
Short name:
K20
Gene namesi
Name:Krt20Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914059 Krt20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament, Keratin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003083581 – 431Keratin, type I cytoskeletal 20Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC2 Publications1
Modified residuei16PhosphoserineCombined sources1
Modified residuei26PhosphoserineBy similarity1

Post-translational modificationi

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages (By similarity). Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.By similarity2 Publications
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-235 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei235 – 236Cleavage; by caspasesBy similarity2

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D312
PeptideAtlasiQ9D312
PRIDEiQ9D312

PTM databases

iPTMnetiQ9D312
PhosphoSitePlusiQ9D312

Expressioni

Tissue specificityi

Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.2 Publications

Gene expression databases

BgeeiENSMUSG00000035775
CleanExiMM_KRT20
GenevisibleiQ9D312 MM

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Associates with KRT8.Curated1 Publication

Protein-protein interaction databases

BioGridi211731, 1 interactor
STRINGi10090.ENSMUSP00000017743

Structurei

3D structure databases

ProteinModelPortaliQ9D312
SMRiQ9D312
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 388IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 76HeadSequence analysisAdd BLAST76
Regioni77 – 112Coil 1ASequence analysisAdd BLAST36
Regioni113 – 130Linker 1Sequence analysisAdd BLAST18
Regioni131 – 222Coil 1BSequence analysisAdd BLAST92
Regioni223 – 245Linker 12Sequence analysisAdd BLAST23
Regioni246 – 384Coil 2Sequence analysisAdd BLAST139
Regioni385 – 431TailSequence analysisAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 16Poly-SerSequence analysis6

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IVBB Eukaryota
ENOG410YGAC LUCA
GeneTreeiENSGT00900000140814
HOGENOMiHOG000230975
HOVERGENiHBG013015
InParanoidiQ9D312
KOiK07604
OMAiKYETERG
OrthoDBiEOG091G087I
PhylomeDBiQ9D312
TreeFamiTF332742

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR002957 Keratin_I
PANTHERiPTHR23239 PTHR23239, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PRINTSiPR01248 TYPE1KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSRQSFHR SLSSSSQGPA LSMSGSLYRK GTVQRLGAAP SVYGGAGGHG
60 70 80 90 100
TRISVSKAVM SYGGDLSNGS DLFGGNGKLA MQNLNDRLAN YLEKVRSLEQ
110 120 130 140 150
SNSRLEAQIK QWYETNAPST IRDYSSYYAQ IKELQNQVKD AQVQNAQCVL
160 170 180 190 200
RIDNAKLAAE DFRLKFETER GMRIAVEADL QGLSKVYDNL TLQKTDLEIQ
210 220 230 240 250
IEELNKDLAL LKKEHQEEVE VLRRQLGNNV NVEVDAAPGL NLGEIMNEMR
260 270 280 290 300
QRYEVLAQKN LQEAKEQFER QSQTLQQQVT VNTEELKGFE VQVTELRRTY
310 320 330 340 350
QNLEIELQSH LSMKESLERN LEDVKARYAS QLAAIQEMLS SLEAQLMQIR
360 370 380 390 400
SDTERQNQEH NILLDIKTRL EQEIATYRRL LEGEDIKTTE YQLSTLEMKD
410 420 430
IKKTRKIKTV VEEVVDGKVV SSEVKEIEES V
Length:431
Mass (Da):49,034
Last modified:June 1, 2001 - v1
Checksum:i5375E42A8BA0ACB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24S → N in AAH57172 (PubMed:15489334).Curated1
Sequence conflicti151R → Q in AAH57172 (PubMed:15489334).Curated1
Sequence conflicti173R → H in AAH57172 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF473907 mRNA Translation: AAL82480.1
AK018567 mRNA Translation: BAB31280.1
AL591165 Genomic DNA Translation: CAM21365.1
BC057172 mRNA Translation: AAH57172.1
CCDSiCCDS25381.1
RefSeqiNP_075745.1, NM_023256.2
UniGeneiMm.28042

Genome annotation databases

EnsembliENSMUST00000017743; ENSMUSP00000017743; ENSMUSG00000035775
GeneIDi66809
KEGGimmu:66809
UCSCiuc007liu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiK1C20_MOUSE
AccessioniPrimary (citable) accession number: Q9D312
Secondary accession number(s): Q6PG82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health