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Q9D312

- K1C20_MOUSE

UniProt

Q9D312 - K1C20_MOUSE

Protein

Keratin, type I cytoskeletal 20

Gene

Krt20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei235 – 2362Cleavage; by caspasesBy similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cellular response to stress Source: UniProtKB
    3. intermediate filament organization Source: UniProtKB
    4. regulation of protein secretion Source: UniProtKB

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Keratin, type I cytoskeletal 20
    Alternative name(s):
    Cytokeratin-20
    Short name:
    CK-20
    Keratin-20
    Short name:
    K20
    Gene namesi
    Name:Krt20Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1914059. Krt20.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. intermediate filament Source: UniProtKB-KW

    Keywords - Cellular componenti

    Intermediate filament, Keratin

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 431431Keratin, type I cytoskeletal 20PRO_0000308358Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC2 Publications

    Post-translational modificationi

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages By similarity. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.By similarity2 Publications
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-235 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9D312.
    PaxDbiQ9D312.
    PRIDEiQ9D312.

    PTM databases

    PhosphoSiteiQ9D312.

    Expressioni

    Tissue specificityi

    Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.2 Publications

    Gene expression databases

    BgeeiQ9D312.
    CleanExiMM_KRT20.
    GenevestigatoriQ9D312.

    Interactioni

    Subunit structurei

    Heterotetramer of two type I and two type II keratins. Associates with KRT8.1 PublicationCurated

    Protein-protein interaction databases

    BioGridi211731. 1 interaction.
    MINTiMINT-1866652.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D312.
    SMRiQ9D312. Positions 294-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7676HeadSequence AnalysisAdd
    BLAST
    Regioni77 – 384308RodSequence AnalysisAdd
    BLAST
    Regioni77 – 11236Coil 1ASequence AnalysisAdd
    BLAST
    Regioni113 – 13018Linker 1Sequence AnalysisAdd
    BLAST
    Regioni131 – 22292Coil 1BSequence AnalysisAdd
    BLAST
    Regioni223 – 24523Linker 12Sequence AnalysisAdd
    BLAST
    Regioni246 – 384139Coil 2Sequence AnalysisAdd
    BLAST
    Regioni385 – 43147TailSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 166Poly-SerSequence Analysis

    Sequence similaritiesi

    Belongs to the intermediate filament family.Sequence Analysis

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG149615.
    GeneTreeiENSGT00750000117520.
    HOGENOMiHOG000230975.
    HOVERGENiHBG013015.
    InParanoidiQ9D312.
    KOiK07604.
    OMAiQIKQWYE.
    OrthoDBiEOG7FV3Q8.
    PhylomeDBiQ9D312.
    TreeFamiTF332742.

    Family and domain databases

    InterProiIPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR002957. Keratin_I.
    [Graphical view]
    PANTHERiPTHR23239. PTHR23239. 1 hit.
    PfamiPF00038. Filament. 1 hit.
    [Graphical view]
    PRINTSiPR01248. TYPE1KERATIN.
    PROSITEiPS00226. IF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9D312-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFSRQSFHR SLSSSSQGPA LSMSGSLYRK GTVQRLGAAP SVYGGAGGHG    50
    TRISVSKAVM SYGGDLSNGS DLFGGNGKLA MQNLNDRLAN YLEKVRSLEQ 100
    SNSRLEAQIK QWYETNAPST IRDYSSYYAQ IKELQNQVKD AQVQNAQCVL 150
    RIDNAKLAAE DFRLKFETER GMRIAVEADL QGLSKVYDNL TLQKTDLEIQ 200
    IEELNKDLAL LKKEHQEEVE VLRRQLGNNV NVEVDAAPGL NLGEIMNEMR 250
    QRYEVLAQKN LQEAKEQFER QSQTLQQQVT VNTEELKGFE VQVTELRRTY 300
    QNLEIELQSH LSMKESLERN LEDVKARYAS QLAAIQEMLS SLEAQLMQIR 350
    SDTERQNQEH NILLDIKTRL EQEIATYRRL LEGEDIKTTE YQLSTLEMKD 400
    IKKTRKIKTV VEEVVDGKVV SSEVKEIEES V 431
    Length:431
    Mass (Da):49,034
    Last modified:June 1, 2001 - v1
    Checksum:i5375E42A8BA0ACB3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241S → N in AAH57172. (PubMed:15489334)Curated
    Sequence conflicti151 – 1511R → Q in AAH57172. (PubMed:15489334)Curated
    Sequence conflicti173 – 1731R → H in AAH57172. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF473907 mRNA. Translation: AAL82480.1.
    AK018567 mRNA. Translation: BAB31280.1.
    AL591165 Genomic DNA. Translation: CAM21365.1.
    BC057172 mRNA. Translation: AAH57172.1.
    CCDSiCCDS25381.1.
    RefSeqiNP_075745.1. NM_023256.1.
    UniGeneiMm.28042.

    Genome annotation databases

    EnsembliENSMUST00000017743; ENSMUSP00000017743; ENSMUSG00000035775.
    GeneIDi66809.
    KEGGimmu:66809.
    UCSCiuc007liu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF473907 mRNA. Translation: AAL82480.1 .
    AK018567 mRNA. Translation: BAB31280.1 .
    AL591165 Genomic DNA. Translation: CAM21365.1 .
    BC057172 mRNA. Translation: AAH57172.1 .
    CCDSi CCDS25381.1.
    RefSeqi NP_075745.1. NM_023256.1.
    UniGenei Mm.28042.

    3D structure databases

    ProteinModelPortali Q9D312.
    SMRi Q9D312. Positions 294-383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211731. 1 interaction.
    MINTi MINT-1866652.

    PTM databases

    PhosphoSitei Q9D312.

    Proteomic databases

    MaxQBi Q9D312.
    PaxDbi Q9D312.
    PRIDEi Q9D312.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000017743 ; ENSMUSP00000017743 ; ENSMUSG00000035775 .
    GeneIDi 66809.
    KEGGi mmu:66809.
    UCSCi uc007liu.1. mouse.

    Organism-specific databases

    CTDi 54474.
    MGIi MGI:1914059. Krt20.

    Phylogenomic databases

    eggNOGi NOG149615.
    GeneTreei ENSGT00750000117520.
    HOGENOMi HOG000230975.
    HOVERGENi HBG013015.
    InParanoidi Q9D312.
    KOi K07604.
    OMAi QIKQWYE.
    OrthoDBi EOG7FV3Q8.
    PhylomeDBi Q9D312.
    TreeFami TF332742.

    Miscellaneous databases

    NextBioi 322699.
    PROi Q9D312.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D312.
    CleanExi MM_KRT20.
    Genevestigatori Q9D312.

    Family and domain databases

    InterProi IPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR002957. Keratin_I.
    [Graphical view ]
    PANTHERi PTHR23239. PTHR23239. 1 hit.
    Pfami PF00038. Filament. 1 hit.
    [Graphical view ]
    PRINTSi PR01248. TYPE1KERATIN.
    PROSITEi PS00226. IF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Keratin 20 helps maintain intermediate filament organization in intestinal epithelia."
      Zhou Q., Toivola D.M., Feng N., Greenberg H.B., Franke W.W., Omary M.B.
      Mol. Biol. Cell 14:2959-2971(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH KRT8, TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported.
      Tissue: ColonImported.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: ColonImported.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 79-87 AND 370-378, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. "Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker."
      Zhou Q., Cadrin M., Herrmann H., Chen C.-H., Chalkley R.J., Burlingame A.L., Omary M.B.
      J. Biol. Chem. 281:16453-16461(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-13.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver1 Publication.
    8. "p38 MAP kinase and MAPKAP kinases MK2/3 cooperatively phosphorylate epithelial keratins."
      Menon M.B., Schwermann J., Singh A.K., Franz-Wachtel M., Pabst O., Seidler U., Omary M.B., Kotlyarov A., Gaestel M.
      J. Biol. Chem. 285:33242-33251(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-13 BY MAPKAPK2 AND MAPKAPK3.

    Entry informationi

    Entry nameiK1C20_MOUSE
    AccessioniPrimary (citable) accession number: Q9D312
    Secondary accession number(s): Q6PG82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3