Q9D309 (FAM3B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein FAM3B

Alternative name(s):
Cytokine-like protein 2-21
Pancreatic-derived factor
Short name=PANDER

Gene names

Name:	Fam3b
Synonyms:	ORF9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Ref.7
Subcellular location	Secreted By similarity. Note: Present in insulin secretory granules and likely cosecreted with insulin By similarity.
Tissue specificity	Expressed at high levels in the pancreas and, to a lesser extent, in small intestine and prostate. Also detected in stomach, testis and fetal liver. In the pancreas, localized in the islets of Langerhans; in the testis, found primarily in the round spermatids; in the CNS, found in the Purkinje cell layer of the cerebellum and in nerve cell bodies of numerous brainstem nuclei.
Induction	By glucose. Ref.6
Post-translational modification	O-glycosylated. Ref.7
Miscellaneous	It is unclear whether the N-terminus residue of the mature protein is Glu-30 or Ser-46.
Sequence similarities	Belongs to the FAM3 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW insulin secretion Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	extracellular region Non-traceable author statement Ref.1. Source: UniProtKB extracellular space Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope lumen Inferred from sequence alignment Ref.3. Source: MGI
Molecular_function	cytokine activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Potential

Chain

30 – 235

206

Protein FAM3B

PRO_0000008751

Amino acid modifications

Glycosylation

208

1

N-linked (GlcNAc...) Potential

Disulfide bond

63 ↔ 91

Ref.7

Disulfide bond

69 ↔ 229

Ref.7

Experimental info

Sequence conflict

162

1

K → R in AAC21456. Ref.5

Secondary structure

1

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235

Helix Strand Turn

Details...

Helix

62 – 64

3

Beta strand

73 – 79

7

Turn

83 – 85

3

Beta strand

89 – 92

4

Beta strand

95 – 99

5

Turn

100 – 103

4

Beta strand

107 – 115

9

Turn

116 – 118

3

Beta strand

121 – 127

7

Beta strand

129 – 133

5

Helix

135 – 144

10

Beta strand

147 – 157

11

Helix

165 – 172

8

Turn

173 – 175

3

Helix

179 – 181

3

Beta strand

187 – 196

10

Beta strand

205 – 208

4

Turn

212 – 214

3

Beta strand

225 – 231

7

Turn

232 – 235

4

Sequences

	Sequence		Length	Mass (Da)
	Q9D309 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 2539E5ED009D9C3C	FASTA	235	26,152
	10 20 30 40 50 60 MRPVATGIFK ALVFIFSSLC AWYSGYLLAE LIPDVPLSST LYNIRSIGER PVLKAPAPKR 70 80 90 100 110 120 QKCDHWSPCP PDTYAYRLLS GGGRDKYAKI CFEDEVLIGE KTGNVARGIN IAVVNYETGK 130 140 150 160 170 180 VIATKYFDMY EGDNSGPMAK FIQSTPSKSL LFMVTHDDGS SKLKAQAKDA IEALGSKEIK 190 200 210 220 230 NMKFRSSWVF VAAKGFELPS EIEREKINHS DQSRNRYAGW PAEIQIEGCI PKGLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and initial characterization of a novel cytokine-like gene family." Zhu Y., Xu G., Patel A., McLaughlin M.M., Silverman C., Knecht K.A., Sweitzer S., Li X., McDonnell P., Mirabile R., Zimmerman D., Boyce R., Tierney L.A., Hu E., Livi G.P., Wolf B.A., Abdel-Meguid S.S., Rose G.D. , Aurora R., Hensley P., Briggs M., Young P.R. Genomics 80:144-150(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Colon.
[3]	"From PREDs and open reading frames to cDNA isolation: revisiting the human chromosome 21 transcription map." Reymond A., Friedli M., Neergaard Henrichsen C., Chapot F., Deutsch S., Ucla C., Rossier C., Lyle R., Guipponi M., Antonarakis S.E. Genomics 78:46-54(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[5]	"Physical and comparative mapping of distal mouse chromosome 16. 5 p5." Cabin D.E., McKee-Johnson J.W., Matesic L.E., Wiltshire T., Rue E.E., Mjaatvedt A.E., Huo Y.K., Korenberg J.R., Reeves R.H. Genome Res. 8:940-950(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-205.
[6]	"Mechanisms of glucose-induced expression of pancreatic-derived factor in pancreatic beta-cells." Wang O., Cai K., Pang S., Wang T., Qi D., Zhu Q., Ni Z., Le Y. Endocrinology 149:672-680(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"FAM3B PANDER and FAM3C ILEI Represent a Distinct Class of Signaling Molecules with a Non-Cytokine-like Fold." Johansson P., Bernstrom J., Gorman T., Oster L., Backstrom S., Schweikart F., Xu B., Xue Y., Schiavone L.H. Structure 21:306-313(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 46-235, PARTIAL PROTEIN SEQUENCE, FUNCTION, GLYCOSYLATION, DISULFIDE BONDS, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF494380 mRNA. Translation: AAM94281.1.
AK018571 mRNA. Translation: BAB31283.1.
AF360358 mRNA. Translation: AAL34461.1.
BC048949 mRNA. Translation: AAH48949.1.
AF045953 Genomic DNA. Translation: AAC21456.1.

IPI

IPI00135156.

RefSeq

NP_065647.1. NM_020622.2.

UniGene

Mm.327932.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YOP	X-ray	2.30	A/B/C	46-235	[»]
2YOQ	X-ray	2.35	A/B/C	30-235	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q9D309.

Proteomic databases

PRIDE

Q9D309.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000049721; ENSMUSP00000062006; ENSMUSG00000022938.

GeneID

52793.

KEGG

mmu:52793.

Organism-specific databases

CTD

54097.

MGI

MGI:1270150. Fam3b.

Phylogenomic databases

eggNOG

NOG75571.

GeneTree

ENSGT00530000063020.

HOGENOM

HOG000285992.

HOVERGEN

HBG099991.

InParanoid

Q9D309.

OMA

FIQSAPP.

OrthoDB

EOG48WC2S.

Gene expression databases

ArrayExpress

Q9D309.

Bgee

Q9D309.

Genevestigator

Q9D309.

GermOnline

ENSMUSG00000022938. Mus musculus.

Family and domain databases

InterPro

IPR026101. FAM3.
[Graphical view]

PANTHER

PTHR14592. PTHR14592. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

FAM3B. mouse.

NextBio

309551.

SOURCE

Search...

Entry information

Entry name

FAM3B_MOUSE

Accession

Primary (citable) accession number: Q9D309
Secondary accession number(s): O88417

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 19, 2001
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program